##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921407_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3054828
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.563934205133645	33.0	31.0	34.0	30.0	34.0
2	31.700112084870245	34.0	31.0	34.0	30.0	34.0
3	31.780709748633967	34.0	31.0	34.0	30.0	34.0
4	35.26808907080857	37.0	35.0	37.0	33.0	37.0
5	35.03445824118412	37.0	35.0	37.0	32.0	37.0
6	34.99426547092013	37.0	35.0	37.0	32.0	37.0
7	34.870069607846986	37.0	35.0	37.0	32.0	37.0
8	34.83295720741069	37.0	35.0	37.0	32.0	37.0
9	36.47275329412982	39.0	37.0	39.0	32.0	39.0
10-11	36.331642730785504	39.0	37.0	39.0	32.0	39.0
12-13	36.27257868528113	39.0	37.0	39.0	32.0	39.0
14-15	37.53717590646674	40.0	38.0	41.0	32.0	41.0
16-17	37.516763627935845	40.0	38.0	41.0	32.0	41.0
18-19	37.48158652467504	40.0	38.0	41.0	32.0	41.0
20-21	37.44708867405956	40.0	38.0	41.0	32.0	41.0
22-23	37.342024330011384	40.0	38.0	41.0	31.5	41.0
24-25	37.28087915260696	40.0	38.0	41.0	31.0	41.0
26-27	37.14968224070226	40.0	37.5	41.0	31.0	41.0
28-29	37.00270375287904	40.0	37.0	41.0	30.5	41.0
30-31	36.86923633671029	40.0	37.0	41.0	30.0	41.0
32-33	36.82559918267084	40.0	37.0	41.0	30.0	41.0
34-35	36.954956874822415	40.0	37.0	41.0	30.0	41.0
36-37	36.93516067025705	40.0	37.0	41.0	30.0	41.0
38-39	36.851163469759996	40.0	37.0	41.0	30.0	41.0
40-41	36.7353937766709	40.0	37.0	41.0	30.0	41.0
42-43	36.64307352165163	40.0	37.0	41.0	30.0	41.0
44-45	36.516090758628636	40.0	37.0	41.0	30.0	41.0
46-47	36.369819348257906	40.0	36.0	41.0	29.5	41.0
48-49	36.219821377832076	40.0	36.0	41.0	29.0	41.0
50-51	36.07555040742065	40.0	36.0	41.0	28.5	41.0
52-53	35.9120808110964	39.5	35.0	41.0	28.0	41.0
54-55	35.7136426338897	39.0	35.0	41.0	28.0	41.0
56-57	35.459976961059674	39.0	35.0	41.0	28.0	41.0
58-59	35.242416103296165	39.0	35.0	41.0	27.0	41.0
60-61	34.97703684135408	38.0	35.0	40.0	26.5	41.0
62-63	34.68911981296492	38.0	34.5	40.0	26.0	41.0
64-65	34.382151302790206	37.0	34.0	40.0	26.0	41.0
66-67	34.04867426251167	37.0	34.0	39.5	26.0	41.0
68-69	33.68376271921038	36.0	34.0	39.0	26.0	41.0
70-71	33.30222323482697	36.0	34.0	39.0	25.0	40.5
72-73	32.89910970437616	35.0	33.0	38.0	24.0	40.0
74-75	32.48404918378384	35.0	33.0	37.0	23.5	39.0
76-77	29.696168000293305	33.0	29.0	34.5	19.0	36.5
78-79	31.35631220481153	34.5	31.5	36.0	20.5	38.0
80-81	31.545682604716205	35.0	32.5	36.0	22.0	37.0
82-83	31.376569319123696	35.0	33.0	35.0	20.5	37.0
84-85	31.135584556642797	35.0	32.0	35.0	20.0	36.5
86-87	30.88619391991955	35.0	32.0	35.0	19.5	36.0
88-89	30.605126868026613	35.0	32.0	35.0	17.5	36.0
90-91	30.42362303213143	35.0	32.0	35.0	13.5	35.5
92-93	30.27710561773036	34.5	32.0	35.0	10.0	35.0
94-95	30.177357939628678	34.0	32.0	35.0	4.5	35.0
96-97	29.98857382477835	34.0	32.0	35.0	2.0	35.0
98-99	29.727917742013624	34.0	31.0	35.0	2.0	35.0
100-101	28.645277410053858	33.5	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	46590.0
3	16060.0
4	8717.0
5	6334.0
6	5607.0
7	5583.0
8	5994.0
9	6553.0
10	7380.0
11	8031.0
12	8632.0
13	9433.0
14	10419.0
15	11394.0
16	12281.0
17	13376.0
18	12279.0
19	11205.0
20	10874.0
21	10775.0
22	11396.0
23	12787.0
24	14899.0
25	18127.0
26	21480.0
27	26609.0
28	31860.0
29	38461.0
30	47064.0
31	58304.0
32	74588.0
33	98659.0
34	141768.0
35	211700.0
36	365059.0
37	720354.0
38	820685.0
39	113509.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.06497724453835	19.623991446661137	13.456131843646487	25.85489946515403
2	15.199046749224992	20.95413462703081	37.87895155508853	25.967867068655657
3	18.06438729277427	24.560504674855302	29.297953277636644	28.07715475473378
4	13.310306582494954	16.627579460950116	35.46568117149004	34.59643278506489
5	14.217116085835832	37.44273114482701	34.27997757706574	14.060175192271423
6	33.71799735955403	35.38174674699087	16.885923633955493	14.014332259499598
7	28.924762228372064	31.997412615203114	20.827923852846418	18.249901303578408
8	26.694797765443596	35.40514838390766	19.659349634748562	18.24070421590018
9	26.83011940443128	15.65780462926227	19.363545181594514	38.14853078471194
10-11	25.685123867275415	25.499198424901653	27.760944482240163	21.054733225582765
12-13	26.049317488204704	22.809403765002116	27.6585126647438	23.482766082049377
14-15	23.288059370944165	24.50107222636621	24.840786538317055	27.37008186437257
16-17	23.579909539767115	27.632582967982106	25.656543767020217	23.13096372523056
18-19	23.59300266693204	27.09227480104399	26.89573300694214	22.418989525081827
20-21	23.99151695275482	26.158926685344387	26.92149744082957	22.928058921071226
22-23	23.85229697733319	25.967446843822827	26.587025286931894	23.59323089191209
24-25	23.67735197215242	26.13948375516794	26.576881445960854	23.60628282671879
26-27	23.475943303886222	26.462136573235973	26.60707516379452	23.45484495908329
28-29	23.581299170162584	26.716142307696717	26.386937618886975	23.315620903253727
30-31	24.027341243337393	26.268661847326445	26.314727144148037	23.389269765188125
32-33	23.676448994284744	26.252202378331233	26.319253881776216	23.752094745607806
34-35	23.935952530462394	26.352465376428015	26.43889744115253	23.272684651957064
36-37	23.869929185158753	26.36351509388102	26.499219670043594	23.267336050916633
38-39	23.86654923474202	26.435931812010498	26.399104758890008	23.298414194357473
40-41	23.990338123132787	26.237966848311085	26.32499707007529	23.44669795848084
42-43	23.73810951814739	26.1784737754637	26.199573487892135	23.88384321849677
44-45	23.69963390949143	26.23015168243674	26.344062876228996	23.726151531842834
46-47	23.735931031423448	26.143732113775826	26.246856943435702	23.873479911365028
48-49	23.54842717528647	26.3366026750469	26.358112004677707	23.756858144988925
50-51	23.68091917402797	26.242402784863955	26.384546473407553	23.692131567700514
52-53	23.861491621888415	26.120921160982206	26.30911829690922	23.70846892022016
54-55	23.368368951641944	26.06146204198251	26.545045888497953	24.02512311787759
56-57	23.50848706071072	26.161196863797816	26.265496865434667	24.064819210056797
58-59	23.51934667526592	26.192376568571923	26.408989271671913	23.87928748449025
60-61	23.69992987547167	26.07190350445857	26.52427546171862	23.703891158351137
62-63	23.59988114989928	26.06670830239984	26.494650517677314	23.838760030023565
64-65	23.306300951782156	25.907696942606538	26.47550794188775	24.310494163723558
66-67	23.3984966247083	26.116479165015644	26.25269266867126	24.2323315416048
68-69	23.35552952756357	26.106516191837727	26.354045888361306	24.1839083922374
70-71	23.307747552905354	25.865616031863564	26.391484709936307	24.43515170529478
72-73	23.33301135228765	26.143873137285077	26.143201888664414	24.379913621762864
74-75	23.23185374418067	26.179795589195447	26.151014097364612	24.437336569259273
76-77	23.191861347554095	25.879963080709672	26.28057840221516	24.647597169521074
78-79	23.14719571672297	25.930349575577143	26.346910322163026	24.575544385536865
80-81	23.278416133835925	26.07161579261026	26.06072526339113	24.58924281016268
82-83	23.238447446872254	26.142682441690855	26.133514723192196	24.48535538824469
84-85	23.205727564748564	26.092301263985267	26.079628501403256	24.62234266986291
86-87	22.95457155311698	26.273904783684245	26.0476283070185	24.72389535618027
88-89	22.914541723771727	26.05431768428968	26.14750198619763	24.88363860574096
90-91	23.022126747833763	25.846723977573077	26.169918867934445	24.961230406658714
92-93	23.01837656331474	25.837272462864313	26.193169759014364	24.951181214806585
94-95	22.87996461806299	25.845264261297306	26.16687061695125	25.10790050368846
96-97	22.732391894643726	25.777269670653606	26.198749768372558	25.29158866633011
98-99	22.544028223369097	25.72739026314062	26.156210926931244	25.572370586559035
100-101	22.534725768562964	25.831300461700827	26.09950202828867	25.534471741447533
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	73.0
1	61.5
2	70.5
3	113.5
4	116.5
5	106.5
6	168.5
7	352.5
8	542.0
9	586.0
10	498.0
11	408.5
12	392.0
13	419.0
14	460.5
15	518.5
16	563.0
17	645.5
18	756.0
19	813.0
20	885.0
21	1030.5
22	1240.0
23	1521.5
24	1873.5
25	2408.0
26	3279.5
27	4619.5
28	6734.5
29	9933.5
30	14245.0
31	19852.5
32	27650.5
33	34815.5
34	40772.5
35	48143.0
36	57294.0
37	66385.0
38	74479.0
39	82147.5
40	89019.5
41	95503.5
42	102329.0
43	109448.5
44	115845.0
45	123146.0
46	128058.5
47	130826.5
48	134585.5
49	136681.0
50	136578.0
51	134014.5
52	130632.5
53	126720.5
54	120958.5
55	113601.0
56	104627.5
57	93835.0
58	82998.0
59	71441.5
60	58897.5
61	48315.0
62	39090.5
63	29818.5
64	21767.0
65	16116.0
66	12387.5
67	9882.0
68	7763.0
69	5970.0
70	4585.5
71	3434.5
72	2473.5
73	1859.5
74	1469.0
75	1115.0
76	787.0
77	506.0
78	319.5
79	177.0
80	103.5
81	68.5
82	39.5
83	19.5
84	18.5
85	14.5
86	9.0
87	8.0
88	5.5
89	4.0
90	4.5
91	3.0
92	2.0
93	1.5
94	1.5
95	1.5
96	0.0
97	0.5
98	0.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15683370716780126
2	5.892312103987524E-4
3	0.0061541926419425245
4	0.003928208069325016
5	0.02658087460243261
6	0.0013421377570193805
7	1.964104034662508E-4
8	0.002717010581283136
9	0.0
10-11	0.10920418432723544
12-13	2.946156051993762E-4
14-15	0.006628851116985964
16-17	1.6367533622187565E-4
18-19	2.291454707106259E-4
20-21	9.656844837090665E-4
22-23	2.946156051993762E-4
24-25	0.0018495312993071949
26-27	0.0028152157830162615
28-29	0.006334235511786588
30-31	0.015320011470367562
32-33	0.015041763398790372
34-35	0.013290437301216306
36-37	9.984195509534417E-4
38-39	2.7824807157718866E-4
40-41	0.004648379548701269
42-43	0.009296759097402537
44-45	0.008363809680937848
46-47	0.009083981160314098
48-49	0.011113555329465358
50-51	0.005646799099654711
52-53	0.0017185910303296943
54-55	0.0010475221518200042
56-57	0.005826841969498774
58-59	0.00975505003882379
60-61	0.008576587618026285
62-63	0.018348005190472264
64-65	0.03545207782565827
66-67	0.0381690884069414
68-69	0.027857542224963238
70-71	0.04285020302288705
72-73	0.02677728500589886
74-75	0.025598822585101353
76-77	0.2501122812806482
78-79	0.0724099687445578
80-81	0.056189742924969914
82-83	0.02072129756568946
84-85	0.03386442706430607
86-87	0.08473472156206503
88-89	0.26769101239087767
90-91	0.32460092679522384
92-93	0.05564961431543773
94-95	0.0053030808935887715
96-97	0.011588213804508798
98-99	0.001784061164818445
100-101	0.0011784624207975048
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3054828.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.43197557936392
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.4515116674972	48.97882860589369
2	12.346388726408893	15.416188789209878
3	3.9753178101403552	7.445608333286794
4	1.769675140003767	4.419372604964904
5	0.9462768490319563	2.953896656504026
6	0.5927602502710169	2.220431608160266
7	0.4010239605413767	1.7525702677881319
8	0.272665689983205	1.3618446158689492
9	0.20690974819690083	1.1626005911905122
>10	0.979633145339658	10.568711875394854
>50	0.04429506000465878	1.8904747108816677
>100	0.012649431985084617	1.3805173268582471
>500	7.350169614701642E-4	0.3187943886282472
>1k	1.5750363445439419E-4	0.1301596253697786
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.273506724437513E-5	0.0
5	0.0	0.0	0.0	3.273506724437513E-5	0.0
6	0.0	0.0	0.0	3.273506724437513E-5	0.0
7	0.0	0.0	0.0	3.273506724437513E-5	0.0
8	0.0	0.0	0.0	3.273506724437513E-5	0.0
9	0.0	0.0	0.0	3.273506724437513E-5	0.0
10-11	0.0	0.0	0.0	3.273506724437513E-5	0.0
12-13	0.0	1.6367533622187566E-5	0.0	3.273506724437513E-5	0.0
14-15	0.0	3.273506724437513E-5	0.0	3.273506724437513E-5	0.0
16-17	0.0	3.273506724437513E-5	0.0	3.273506724437513E-5	3.273506724437513E-5
18-19	0.0	3.273506724437513E-5	0.0	3.273506724437513E-5	3.273506724437513E-5
20-21	0.0	3.273506724437513E-5	0.0	6.547013448875026E-5	6.547013448875026E-5
22-23	0.0	3.273506724437513E-5	0.0	1.964104034662508E-4	1.1457273535531296E-4
24-25	0.0	3.273506724437513E-5	0.0	5.237610759100022E-4	1.3094026897750053E-4
26-27	0.0	3.273506724437513E-5	0.0	8.511117483537534E-4	1.3094026897750053E-4
28-29	0.0	3.273506724437513E-5	0.0	0.0037972678003475157	1.3094026897750053E-4
30-31	0.0	3.273506724437513E-5	0.0	0.01715317523605257	1.6367533622187568E-4
32-33	0.0	3.273506724437513E-5	0.0	0.04437238364975049	1.6367533622187568E-4
34-35	0.0	3.273506724437513E-5	0.0	0.08314707080071285	1.6367533622187568E-4
36-37	0.0	3.273506724437513E-5	0.0	0.1427085256518534	1.6367533622187568E-4
38-39	3.273506724437513E-5	3.273506724437513E-5	0.0	0.23495594514650253	1.6367533622187568E-4
40-41	3.273506724437513E-5	3.273506724437513E-5	0.0	0.33481426777546885	1.964104034662508E-4
42-43	3.273506724437513E-5	3.273506724437513E-5	0.0	0.4443294352415259	1.964104034662508E-4
44-45	3.273506724437513E-5	3.273506724437513E-5	0.0	0.5818330852015237	1.964104034662508E-4
46-47	3.273506724437513E-5	3.273506724437513E-5	0.0	0.7368827312045064	2.1277793708843835E-4
48-49	3.273506724437513E-5	3.273506724437513E-5	0.0	0.8913758810643349	2.2914547071062593E-4
50-51	3.273506724437513E-5	3.273506724437513E-5	0.0	1.0540364302016347	2.2914547071062593E-4
52-53	3.273506724437513E-5	3.273506724437513E-5	0.0	1.227073995655402	2.6188053795500105E-4
54-55	3.273506724437513E-5	3.273506724437513E-5	0.0	1.4342869713122965	2.946156051993762E-4
56-57	3.273506724437513E-5	3.273506724437513E-5	0.0	1.6676552656974468	2.946156051993762E-4
58-59	3.273506724437513E-5	3.273506724437513E-5	0.0	1.9097638230368452	3.2735067244375136E-4
60-61	3.273506724437513E-5	3.273506724437513E-5	0.0	2.15887768476654	3.6008573968812646E-4
62-63	3.273506724437513E-5	3.273506724437513E-5	0.0	2.423098780029514	3.928208069325016E-4
64-65	3.273506724437513E-5	3.273506724437513E-5	0.0	2.7194984463937084	4.255558741768767E-4
66-67	3.273506724437513E-5	3.273506724437513E-5	0.0	3.0667847747892845	4.5829094142125186E-4
68-69	3.273506724437513E-5	3.273506724437513E-5	0.0	3.439391677698384	4.5829094142125186E-4
70-71	6.547013448875026E-5	3.273506724437513E-5	0.0	3.8183328161192707	4.5829094142125186E-4
72-73	6.547013448875026E-5	3.273506724437513E-5	0.0	4.207454560453158	4.91026008665627E-4
74-75	6.547013448875026E-5	3.273506724437513E-5	0.0	4.6287057732873995	4.91026008665627E-4
76-77	6.547013448875026E-5	3.273506724437513E-5	0.0	5.122465160067932	4.91026008665627E-4
78-79	6.547013448875026E-5	6.547013448875026E-5	0.0	5.655490260008092	5.564961431543773E-4
80-81	6.547013448875026E-5	6.547013448875026E-5	0.0	6.2007910101648935	5.564961431543773E-4
82-83	8.183766811093783E-5	6.547013448875026E-5	0.0	6.755912280495007	5.728636767765649E-4
84-85	1.3094026897750053E-4	6.547013448875026E-5	0.0	7.353409095372964	5.892312103987524E-4
86-87	1.3094026897750053E-4	6.547013448875026E-5	0.0	8.013888179629099	6.219662776431275E-4
88-89	1.3094026897750053E-4	6.547013448875026E-5	0.0	8.723993625827706	6.219662776431275E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2515	0.0	47.43452	1
GTATCAA	4380	0.0	40.47562	1
TCAACGC	5670	0.0	31.050343	4
ATCAACG	5725	0.0	31.000711	3
CAACGCA	5685	0.0	30.861609	5
TATCAAC	6070	0.0	29.239206	2
AACGCAG	6065	0.0	29.081915	6
GTACATG	8480	0.0	27.40765	1
TACATGG	8905	0.0	25.89912	2
ACATGGG	9135	0.0	23.895979	3
ACGCAGA	7520	0.0	23.012102	7
CATGGGG	7565	0.0	22.080519	4
CGCAGAG	7745	0.0	21.975279	8
ATGGGGG	4730	0.0	18.898153	5
AGAGTAC	7655	0.0	18.041544	10-11
GCAGAGT	9185	0.0	17.857174	9
GAGTACA	6595	0.0	17.296381	1
AGTACAC	2560	0.0	17.239256	3
GTACACT	2775	0.0	16.587627	4
TACACTG	3615	0.0	16.57238	5
>>END_MODULE