FastQCFastQC Report
Mon 15 Aug 2016
SRR2921406_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921406_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2837944
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23600.055.4901581
GTATCAA45650.041.15981
TCAACGC53150.034.9115374
CAACGCA53650.034.4068345
TATCAAC55750.033.1959082
ATCAACG57650.032.2670483
AACGCAG58400.031.840316
GTACATG102050.030.8726541
TACATGG106400.028.677032
ACATGGG110300.027.0182113
ACGCAGA68500.026.5963027
CGCAGAG71150.025.2078488
CATGGGG94100.024.6612264
GAGTACA74250.023.5163691
ATGGGGG63400.022.454165
AGTACAT75650.020.7627032
GCAGAGT82050.020.470499
GTAAGTA18200.019.815592
TAAGTAC23850.018.3050923
TACACTG41350.018.1320295
Produced by FastQC (version 0.11.5)