##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921406_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2837944
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.48073006373628	31.0	31.0	34.0	27.0	34.0
2	30.52512664097671	31.0	31.0	34.0	26.0	34.0
3	30.578975836027773	33.0	31.0	34.0	26.0	34.0
4	34.049213444662755	37.0	35.0	37.0	30.0	37.0
5	34.04554247722999	37.0	35.0	37.0	30.0	37.0
6	34.08971530093617	37.0	35.0	37.0	30.0	37.0
7	34.01062494538299	37.0	35.0	37.0	30.0	37.0
8	33.97675042213659	37.0	35.0	37.0	30.0	37.0
9	35.54052898859174	39.0	37.0	39.0	30.0	39.0
10-11	35.429322072599035	39.0	36.0	39.0	30.0	39.0
12-13	35.31091275937791	39.0	35.0	39.0	29.0	39.0
14-15	36.44025674925228	40.0	37.0	41.0	29.0	41.0
16-17	36.390059493774366	40.0	37.0	41.0	29.0	41.0
18-19	36.33762258874735	40.0	37.0	41.0	28.0	41.0
20-21	36.273300142638476	40.0	37.0	41.0	28.0	41.0
22-23	36.15928837919283	40.0	36.0	41.0	27.0	41.0
24-25	36.06255778831436	40.0	36.0	41.0	27.0	41.0
26-27	35.915207629185076	40.0	36.0	41.0	27.0	41.0
28-29	35.770476091142044	40.0	36.0	41.0	27.0	41.0
30-31	35.62567584138377	40.0	36.0	41.0	26.5	41.0
32-33	35.44791158669798	39.0	35.0	41.0	25.0	41.0
34-35	35.29063593221008	39.0	35.0	41.0	25.0	41.0
36-37	35.12194461906225	39.0	35.0	41.0	25.0	41.0
38-39	35.36536732225865	39.0	35.0	41.0	25.0	41.0
40-41	35.49154810665749	40.0	35.0	41.0	25.0	41.0
42-43	35.33723938879696	40.0	35.0	41.0	24.5	41.0
44-45	35.22794336322352	40.0	35.0	41.0	24.0	41.0
46-47	35.08182684365865	40.0	35.0	41.0	23.5	41.0
48-49	34.94235457077377	39.0	35.0	41.0	23.0	41.0
50-51	33.223220049444244	37.0	32.5	39.5	19.0	40.0
52-53	33.38422833572474	37.5	33.5	39.5	20.5	40.0
54-55	34.25517469689325	39.0	34.0	40.5	21.5	41.0
56-57	34.27494887143651	39.0	34.0	41.0	21.0	41.0
58-59	34.10051907296268	38.0	34.0	41.0	20.0	41.0
60-61	33.850427985894015	38.0	34.0	40.0	19.5	41.0
62-63	33.55899076937388	37.0	34.0	40.0	18.0	41.0
64-65	33.27155680309407	37.0	33.5	40.0	17.5	41.0
66-67	32.935547177816055	36.0	33.0	39.5	13.5	41.0
68-69	32.564780876578254	36.0	33.0	39.0	12.0	41.0
70-71	32.18176415743228	35.0	33.0	39.0	8.0	40.5
72-73	31.82722509676019	35.0	32.0	37.5	7.0	40.0
74-75	31.42839270260442	35.0	32.0	37.0	2.0	39.0
76-77	31.05401146040937	35.0	32.0	36.5	2.0	39.0
78-79	30.710600702480388	35.0	31.5	36.0	2.0	38.5
80-81	30.367137089385835	35.0	31.0	36.0	2.0	37.0
82-83	30.067527583349076	35.0	31.0	35.0	2.0	37.0
84-85	29.836148105811812	35.0	31.0	35.0	2.0	36.5
86-87	29.61383646047984	35.0	31.0	35.0	2.0	36.0
88-89	29.41984443667669	35.0	31.0	35.0	2.0	36.0
90-91	29.17641063389552	34.5	31.0	35.0	2.0	35.5
92-93	28.985102771583932	34.0	30.0	35.0	2.0	35.0
94-95	28.77501494039347	34.0	30.0	35.0	2.0	35.0
96-97	28.52280982288586	34.0	30.0	35.0	2.0	35.0
98-99	28.30506662569804	34.0	30.0	35.0	2.0	35.0
100-101	27.257442007312335	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	91469.0
3	15738.0
4	11271.0
5	8603.0
6	7654.0
7	7904.0
8	8341.0
9	9051.0
10	9892.0
11	10385.0
12	10492.0
13	10429.0
14	11476.0
15	12051.0
16	12042.0
17	12725.0
18	12196.0
19	11414.0
20	11954.0
21	12304.0
22	13482.0
23	15577.0
24	18156.0
25	21374.0
26	25190.0
27	30128.0
28	35751.0
29	42356.0
30	51210.0
31	61436.0
32	77050.0
33	101172.0
34	143935.0
35	205961.0
36	335243.0
37	608763.0
38	641614.0
39	112148.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.09964153864811	17.404447910291594	12.376399931184444	25.11951061987585
2	16.340252037763005	19.439090286325136	39.06906164711258	25.15159602879928
3	19.971479890502522	23.488090474671218	28.762759576182006	27.777670058644254
4	13.73153829556496	15.045381277473089	35.96928090841528	35.25379951854667
5	14.51347025685499	37.4371963816091	33.63185763338058	14.41747572815534
6	34.15910359644078	34.11963184130099	17.149490814358696	14.571773747899538
7	29.71892857709244	31.679750205724332	20.21701934954106	18.38430186764216
8	27.563288562858233	34.32566388274175	19.61488157481547	18.49616597958455
9	27.907839811881736	15.515985964437414	18.66059483379644	37.915579389884414
10-11	26.459829864426904	24.881618270445177	27.2103077352246	21.44824412990332
12-13	26.791014448516957	22.18927476149708	27.180345110650485	23.83936567933548
14-15	23.979819625965444	23.753730765834373	24.48069801828981	27.78575158991038
16-17	24.19046340076307	26.842060462059607	25.257185899059305	23.710290238118024
18-19	24.168120971364516	26.43736497787571	26.486161944956528	22.908352105803246
20-21	24.575575823603366	25.582555599769773	26.583279201268013	23.25858937535885
22-23	24.478283224703482	25.288472673732702	26.257160589511358	23.97608351205246
24-25	24.234602106253174	25.434922121087084	26.233020362961966	24.097455409697773
26-27	23.985766353827465	25.744669564363786	26.208103717888864	24.06146036391988
28-29	23.961938112603214	26.267869043931036	25.845912724384018	23.924280119081732
30-31	24.65627279679444	25.702308665513097	25.829953093668173	23.81146544402429
32-33	24.266522838632557	25.735115842415972	25.81292676188167	24.185434557069797
34-35	24.53032354167845	25.942379165453083	25.92181653161756	23.605480761250906
36-37	24.41498872050894	25.946159370293604	26.031698153630828	23.607153755566628
38-39	24.409603234132614	25.977581036119872	25.89682991502191	23.715985814725602
40-41	24.48311002953657	25.678632818363837	25.94300661278057	23.895250539319015
42-43	24.177858721856968	25.691869693452553	25.777334209874365	24.352937374816115
44-45	23.999651874756825	25.807431661884944	26.10173165828365	24.09118480507458
46-47	24.16945401415112	25.622417370874285	25.799474108475458	24.408654506499143
48-49	23.980162529976525	25.803999412474187	26.017666025472636	24.198172032076652
50-51	24.161757584648676	25.775703808685	26.08176783797902	23.980770768687304
52-53	24.339102132245248	25.588072872309624	26.000906737397834	24.071918258047294
54-55	23.61537343874933	25.51306968977042	26.465422226867307	24.40613464461294
56-57	23.85505941345763	25.67643060190335	26.026890548581317	24.441619436057703
58-59	24.07460210825904	25.688556413717368	26.180477637817656	24.056363840205936
60-61	24.308006971442758	25.653493304791215	26.29065887761608	23.74784084614995
62-63	24.141454928557643	25.570884690959083	26.3340476362412	23.95361274424207
64-65	23.81429683761876	25.363635882106795	26.359403718724455	24.46266356154999
66-67	23.89927458066384	25.559423830969703	26.140906835960603	24.400394752405855
68-69	23.87645274602122	25.526665947550285	26.28489825591916	24.311983050509333
70-71	23.75539557170378	25.33160679745835	26.303312767175015	24.609684863662856
72-73	23.688311037229138	25.7712460839935	26.0771301689653	24.46331270981207
74-75	23.666421414081217	25.691820590908094	26.254883574455224	24.38687442055546
76-77	23.664284781234883	25.194828578388627	26.279192512594925	24.861694127781565
78-79	23.51432015567893	25.27458964507683	26.544928171886717	24.666162027357526
80-81	23.80654877998605	25.477568550570908	26.109317502409056	24.60656516703399
82-83	23.814886003349752	25.518333140565115	26.30378564409454	24.362995211990597
84-85	23.713585422921422	25.56079371298684	26.14244519637381	24.583175667717928
86-87	23.367950360578092	25.808985003801364	26.20289681029451	24.620167825326032
88-89	23.303469678568508	25.39526178571246	26.392168989280908	24.909099546438128
90-91	23.600474979179044	25.210505347047736	26.486835292281025	24.702184381492202
92-93	23.762580996095235	25.001474663167507	26.519212123673906	24.716732217063353
94-95	23.588676132660506	25.065996428615584	26.514473644702	24.830853794021905
96-97	23.39697338259591	24.845618773661453	26.664157379300747	25.09325046444189
98-99	23.08701963229818	24.80340415852282	26.614556648936972	25.49501956024203
100-101	23.326072185103996	24.89927386751434	26.70034242734304	25.074311520038634
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	62.0
1	50.5
2	94.0
3	144.5
4	129.0
5	140.5
6	235.0
7	437.0
8	664.0
9	725.5
10	609.5
11	510.0
12	522.0
13	610.0
14	714.5
15	797.0
16	847.0
17	928.0
18	1088.0
19	1205.0
20	1279.5
21	1405.5
22	1515.0
23	1607.5
24	1822.0
25	2117.0
26	2487.5
27	3082.0
28	3946.0
29	5477.0
30	8343.5
31	12872.5
32	19377.0
33	25662.5
34	31680.5
35	38882.5
36	47859.0
37	56675.5
38	63700.0
39	70452.5
40	76101.0
41	81105.5
42	86849.5
43	93413.5
44	99757.5
45	106511.5
46	112425.0
47	117629.0
48	123817.5
49	128769.0
50	130227.5
51	130718.0
52	129853.5
53	126890.0
54	123346.0
55	116792.0
56	107973.0
57	97789.0
58	87145.5
59	74195.0
60	60598.0
61	50015.5
62	40894.5
63	31463.5
64	22803.5
65	16835.0
66	12546.0
67	9660.5
68	7536.0
69	5757.5
70	4384.0
71	3205.0
72	2355.0
73	1861.5
74	1496.5
75	1165.5
76	874.5
77	606.0
78	402.5
79	304.0
80	227.0
81	164.5
82	120.0
83	97.5
84	87.0
85	68.0
86	59.0
87	43.5
88	43.5
89	42.0
90	35.0
91	30.5
92	23.5
93	20.5
94	20.0
95	19.0
96	14.5
97	8.5
98	7.5
99	7.5
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04848580521673437
2	0.05718928914735456
3	0.047534412236464145
4	0.053982742436073435
5	0.04665349281028801
6	0.016631758766205394
7	0.014411841812241538
8	0.020543041018427424
9	0.020437330687286287
10-11	0.021793946603597535
12-13	0.027026607995083763
14-15	0.04325314382524814
16-17	0.029686984662135686
18-19	0.02387291644937321
20-21	0.026321872454142862
22-23	0.046635874421764485
24-25	0.04230175084497791
26-27	0.03042695698012364
28-29	0.01990877903158061
30-31	0.013178554615594952
32-33	0.010430086005925416
34-35	0.00960202174531985
36-37	0.0017618388523522664
38-39	0.0074878151224971315
40-41	0.0037350983669868047
42-43	0.22408828363068475
44-45	0.19838305477486518
46-47	0.20440854364990993
48-49	0.20183625892547563
50-51	0.20062059011735256
52-53	0.20479614819742745
54-55	0.20435568848433935
56-57	0.2147329193246942
58-59	0.2109449657921368
60-61	0.21513814226073522
62-63	0.21302393563791253
64-65	0.21349963212804762
66-67	0.20398570232534538
68-69	0.20312240128769277
70-71	0.1866492080181991
72-73	0.19374941859317873
74-75	0.19344990598827883
76-77	0.1921813820145852
78-79	0.21911989806705137
80-81	0.2269072257944484
82-83	0.2252158604961902
84-85	0.2207760265882625
86-87	0.2370201808069504
88-89	0.23564594650211562
90-91	0.23474740868741598
92-93	0.2392224793723907
94-95	0.25049824802744525
96-97	0.25113251001429204
98-99	0.24026196429527855
100-101	0.25104441807167444
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2837944.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.36936917144134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.48631295048823	46.596828884628124
2	11.991949703983803	14.239089781223422
3	3.875205219089893	6.902054678017322
4	1.7454207974274116	4.1449812672791815
5	0.9702816791274586	2.8802505604202038
6	0.6146979984723797	2.1896539440151677
7	0.44038727245902587	1.830186018991682
8	0.3054229425493031	1.4506213943709996
9	0.23042774087130277	1.2312314653616636
>10	1.2564053727846536	13.196935136115817
>50	0.061613439881939784	2.4595475855991893
>100	0.020384489133680263	2.059012137664902
>500	9.538519878215912E-4	0.4135729803037981
>1k	5.365417431144106E-4	0.40603416600857023
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.047355409409065E-5
2	0.0	0.0	0.0	0.0	7.047355409409065E-5
3	0.0	0.0	0.0	0.0	7.047355409409065E-5
4	0.0	0.0	0.0	0.0	7.047355409409065E-5
5	0.0	0.0	0.0	0.0	7.047355409409065E-5
6	0.0	0.0	0.0	0.0	7.047355409409065E-5
7	0.0	0.0	0.0	0.0	7.047355409409065E-5
8	0.0	0.0	0.0	0.0	7.047355409409065E-5
9	0.0	0.0	0.0	0.0	7.047355409409065E-5
10-11	0.0	0.0	0.0	0.0	7.047355409409065E-5
12-13	0.0	0.0	0.0	0.0	1.0571033114113597E-4
14-15	0.0	0.0	0.0	0.0	1.7618388523522662E-4
16-17	0.0	0.0	0.0	1.7618388523522663E-5	2.818942163763626E-4
18-19	0.0	0.0	0.0	3.5236777047045326E-5	3.1713099342340793E-4
20-21	0.0	0.0	0.0	8.809194261761331E-5	3.1713099342340793E-4
22-23	0.0	0.0	0.0	2.466574393293173E-4	3.876045475174986E-4
24-25	0.0	0.0	0.0	4.404597130880666E-4	4.933148786586346E-4
26-27	0.0	3.5236777047045326E-5	0.0	0.001145195254028973	5.461700442292026E-4
28-29	0.0	3.5236777047045326E-5	0.0	0.0031889283227576016	5.637884327527252E-4
30-31	0.0	3.5236777047045326E-5	0.0	0.012931897176265635	5.637884327527252E-4
32-33	0.0	3.5236777047045326E-5	0.0	0.03469060700281612	5.637884327527252E-4
34-35	0.0	3.5236777047045326E-5	0.0	0.0650647088173692	5.990252097997705E-4
36-37	0.0	3.5236777047045326E-5	0.0	0.11089013736705164	5.990252097997705E-4
38-39	0.0	3.5236777047045326E-5	0.0	0.1876534561640399	5.990252097997705E-4
40-41	0.0	3.5236777047045326E-5	0.0	0.27430069092272436	5.990252097997705E-4
42-43	0.0	3.5236777047045326E-5	0.0	0.36441874822054277	5.990252097997705E-4
44-45	0.0	3.5236777047045326E-5	0.0	0.47608409468262936	6.342619868468159E-4
46-47	0.0	3.5236777047045326E-5	0.0	0.6038702666437392	6.342619868468159E-4
48-49	0.0	3.5236777047045326E-5	0.0	0.7333125671260603	6.342619868468159E-4
50-51	0.0	3.5236777047045326E-5	0.0	0.8785057069484106	6.342619868468159E-4
52-53	0.0	3.5236777047045326E-5	0.0	1.0287377058884883	7.223539294644292E-4
54-55	0.0	3.5236777047045326E-5	0.0	1.1993013251847113	8.104458720820425E-4
56-57	0.0	3.5236777047045326E-5	0.0	1.3980191293415234	8.104458720820425E-4
58-59	0.0	3.5236777047045326E-5	0.0	1.6140734278054816	8.280642606055651E-4
60-61	0.0	3.5236777047045326E-5	0.0	1.8405578122753656	9.161562032231785E-4
62-63	0.0	3.5236777047045326E-5	0.0	2.082440668314808	9.513929802702238E-4
64-65	0.0	3.5236777047045326E-5	0.0	2.344461342436637	9.866297573172692E-4
66-67	0.0	3.5236777047045326E-5	0.0	2.6470395469396153	0.0010218665343643145
68-69	0.0	3.5236777047045326E-5	0.0	2.976926253654054	0.0010218665343643145
70-71	0.0	5.285516557056799E-5	0.0	3.3289416563540364	0.001145195254028973
72-73	0.0	7.047355409409065E-5	0.0	3.6929728000270616	0.001303760750740677
74-75	0.0	7.047355409409065E-5	0.0	4.068755408845277	0.0013213791392641997
76-77	0.0	7.047355409409065E-5	0.0	4.4999478495699705	0.001356615916311245
78-79	0.0	7.047355409409065E-5	0.0	4.979855134562204	0.0013742343048347677
80-81	0.0	8.809194261761331E-5	0.0	5.466527880747471	0.0014270894704053357
82-83	0.0	1.0571033114113597E-4	0.0	5.974712679319959	0.0014799446359759036
84-85	0.0	1.0571033114113597E-4	0.0	6.515614825380627	0.0014799446359759036
86-87	1.7618388523522663E-5	1.0571033114113597E-4	0.0	7.092458484029283	0.0014799446359759036
88-89	3.5236777047045326E-5	1.0571033114113597E-4	0.0	7.7177879478946725	0.0014799446359759036
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2360	0.0	55.490158	1
GTATCAA	4565	0.0	41.1598	1
TCAACGC	5315	0.0	34.911537	4
CAACGCA	5365	0.0	34.406834	5
TATCAAC	5575	0.0	33.195908	2
ATCAACG	5765	0.0	32.267048	3
AACGCAG	5840	0.0	31.84031	6
GTACATG	10205	0.0	30.872654	1
TACATGG	10640	0.0	28.67703	2
ACATGGG	11030	0.0	27.018211	3
ACGCAGA	6850	0.0	26.596302	7
CGCAGAG	7115	0.0	25.207848	8
CATGGGG	9410	0.0	24.661226	4
GAGTACA	7425	0.0	23.516369	1
ATGGGGG	6340	0.0	22.45416	5
AGTACAT	7565	0.0	20.762703	2
GCAGAGT	8205	0.0	20.47049	9
GTAAGTA	1820	0.0	19.81559	2
TAAGTAC	2385	0.0	18.305092	3
TACACTG	4135	0.0	18.132029	5
>>END_MODULE