FastQCFastQC Report
Mon 15 Aug 2016
SRR2921406_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921406_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2837944
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24000.052.872561
GTATCAA45550.040.170081
TCAACGC57050.031.6908024
CAACGCA58600.031.075865
TATCAAC58200.030.8200052
ATCAACG58750.030.7737923
AACGCAG61450.029.6309226
GTACATG97600.028.2428471
TACATGG102450.026.3087522
ACATGGG105300.024.5602073
ACGCAGA76200.023.396167
CATGGGG87100.023.0999814
CGCAGAG80550.021.7785598
TAAGGCG7650.020.5104185
TATACCG2351.12160706E-720.2327145
ATGGGGG58800.019.81125
GTATAGC8000.019.0103591
TATATAC9000.018.9812093
GCAGAGT91300.018.2249189
GAGTACA74650.017.8898541
Produced by FastQC (version 0.11.5)