##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921406_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2837944
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.454007901494887	33.0	31.0	34.0	30.0	34.0
2	31.580783482690286	34.0	31.0	34.0	29.0	34.0
3	31.658424197235746	34.0	31.0	34.0	28.0	34.0
4	35.15411509177066	37.0	35.0	37.0	33.0	37.0
5	34.904883253510285	37.0	35.0	37.0	32.0	37.0
6	34.86610236142785	37.0	35.0	37.0	32.0	37.0
7	34.735487733373176	37.0	35.0	37.0	32.0	37.0
8	34.698284391799135	37.0	35.0	37.0	32.0	37.0
9	36.323049714864	39.0	37.0	39.0	32.0	39.0
10-11	36.17264223677422	39.0	37.0	39.0	32.0	39.0
12-13	36.10185384207722	39.0	37.0	39.0	32.0	39.0
14-15	37.33734650859918	40.0	38.0	41.0	32.0	41.0
16-17	37.31494684179815	40.0	38.0	41.0	32.0	41.0
18-19	37.27775283797003	40.0	38.0	41.0	31.0	41.0
20-21	37.23782446024305	40.0	38.0	41.0	31.0	41.0
22-23	37.13492338115199	40.0	38.0	41.0	31.0	41.0
24-25	37.06576997290996	40.0	37.0	41.0	31.0	41.0
26-27	36.92885976608418	40.0	37.0	41.0	30.5	41.0
28-29	36.774933719622375	40.0	37.0	41.0	30.0	41.0
30-31	36.638884523443735	40.0	37.0	41.0	30.0	41.0
32-33	36.59716611744277	40.0	37.0	41.0	30.0	41.0
34-35	36.733186771832	40.0	37.0	41.0	30.0	41.0
36-37	36.713615913492305	40.0	37.0	41.0	30.0	41.0
38-39	36.63057516286438	40.0	37.0	41.0	30.0	41.0
40-41	36.512589219519484	40.0	37.0	41.0	30.0	41.0
42-43	36.41182172727862	40.0	36.5	41.0	30.0	41.0
44-45	36.28807157576048	40.0	36.0	41.0	29.0	41.0
46-47	36.141121882602334	40.0	36.0	41.0	28.5	41.0
48-49	35.9879849637625	40.0	35.5	41.0	28.0	41.0
50-51	35.836863060018096	40.0	35.0	41.0	28.0	41.0
52-53	35.67252877435214	39.0	35.0	41.0	28.0	41.0
54-55	35.46551270920075	39.0	35.0	41.0	27.0	41.0
56-57	35.20539975418825	39.0	35.0	41.0	26.5	41.0
58-59	34.979884557271035	38.5	35.0	40.5	26.0	41.0
60-61	34.70288155791658	38.0	34.5	40.0	26.0	41.0
62-63	34.401096357081045	37.5	34.0	40.0	26.0	41.0
64-65	34.09035414370403	37.0	34.0	40.0	26.0	41.0
66-67	33.75743707416355	36.5	34.0	39.0	25.0	41.0
68-69	33.388752737897576	36.0	34.0	39.0	24.5	41.0
70-71	33.010211970356	35.5	33.5	39.0	23.5	40.0
72-73	32.6135565042862	35.0	33.0	37.5	22.5	40.0
74-75	32.20712512297635	35.0	33.0	37.0	21.5	39.0
76-77	29.44858337585238	33.0	29.0	34.5	16.5	36.5
78-79	31.114643206490335	34.5	31.5	36.0	19.5	37.0
80-81	31.314696484497226	35.0	32.0	36.0	20.0	37.0
82-83	31.151304430249503	35.0	32.0	35.0	20.0	37.0
84-85	30.92901057948994	35.0	32.0	35.0	18.5	36.5
86-87	30.692879246384003	35.0	32.0	35.0	17.5	36.0
88-89	30.42238148462408	35.0	32.0	35.0	11.0	36.0
90-91	30.237951488824304	35.0	32.0	35.0	7.0	35.0
92-93	30.094592599431138	34.0	31.5	35.0	4.5	35.0
94-95	30.001204921591125	34.0	31.0	35.0	2.0	35.0
96-97	29.818814078079058	34.0	31.0	35.0	2.0	35.0
98-99	29.5650738703794	34.0	31.0	35.0	2.0	35.0
100-101	28.470042220706258	33.5	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	48677.0
3	16823.0
4	9374.0
5	6697.0
6	5787.0
7	5917.0
8	6136.0
9	6558.0
10	7380.0
11	7750.0
12	8552.0
13	9227.0
14	9899.0
15	10636.0
16	11468.0
17	12501.0
18	11598.0
19	10426.0
20	10237.0
21	10033.0
22	10567.0
23	11865.0
24	14255.0
25	16950.0
26	20550.0
27	24838.0
28	29900.0
29	36206.0
30	44543.0
31	54996.0
32	70505.0
33	93919.0
34	138559.0
35	203168.0
36	349489.0
37	681190.0
38	719311.0
39	91456.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.893859665840125	18.93294991978102	13.024555548732414	26.148634865646443
2	15.371322941266428	20.688888466046073	37.65232031470781	26.287468277979688
3	18.511034846568606	24.56251940335114	28.700503174485153	28.225942575595102
4	13.338776470986222	16.176655070927584	35.05643760853386	35.42813084955233
5	14.227749733716527	37.71109705970883	33.7498704709781	14.311282735596548
6	34.10373705119197	34.77574662444775	16.81732939709772	14.303186927262566
7	29.46690834465612	31.826193402529164	20.35553303527634	18.35136521753838
8	27.498361464463162	34.87120758307199	19.325029070791782	18.305401881673067
9	27.5485351367046	15.472045960033038	18.648641410824172	38.33077749243819
10-11	26.276986560226945	25.207145279747323	27.200967650961328	21.314900509064408
12-13	26.62505186434138	22.35690947547202	27.30884191220193	23.709196747984667
14-15	23.78494151534175	24.025180813768998	24.446370430850283	27.743507240038966
16-17	24.079322409200767	27.10967509144847	25.257079350246325	23.55392314910444
18-19	24.044634606355157	26.685148431120194	26.52168436000504	22.748532602519617
20-21	24.467735359793313	25.691624255925376	26.631431953179813	23.209208431101498
22-23	24.285030942365715	25.434122492126765	26.33845882793402	23.9423877375735
24-25	24.081182550269116	25.613917753635125	26.238693005621585	24.066206690474175
26-27	23.825549531625807	25.878707055714916	26.35697430641558	23.93876910624369
28-29	23.86457861639653	26.44309634268175	25.889098171605173	23.80322686931655
30-31	24.515418280969907	25.849856098158803	25.892393175812227	23.742332445059063
32-33	24.13661918099328	25.894032558332135	25.835090294438196	24.13425796623639
34-35	24.358902522312604	26.093410919096684	26.022418676968208	23.525267881622504
36-37	24.297628439974194	26.04477009754676	26.126837216398908	23.530764246080142
38-39	24.361089249561875	26.044548933546942	25.937464047368238	23.65689776952295
40-41	24.452104164915745	25.78472638112389	25.945908231004534	23.817261222955832
42-43	24.093732363556146	25.847919254226092	25.768752930413807	24.289595451803955
44-45	23.918684907717584	25.882245563487636	26.101577966960587	24.09749156183419
46-47	24.037064459301018	25.800230542797546	25.810133099524922	24.35257189837651
48-49	23.883992249093293	26.01887473003187	25.98566008253091	24.111472938343923
50-51	24.068210698133676	25.8920044852183	26.061292580149313	23.97849223649871
52-53	24.262747739470107	25.708137273332554	25.978796537069588	24.050318450127744
54-55	23.501713316794202	25.639919285856365	26.42785649260646	24.43051090474297
56-57	23.76056891681718	25.765454136358695	25.98185847311429	24.492118473709834
58-59	23.948364561196207	25.817983703846494	26.090602356480957	24.14304937847634
60-61	24.191475217115745	25.837112930141494	26.16741395231973	23.803997900423035
62-63	24.020518640173115	25.723280303938083	26.209214709137175	24.046986346751627
64-65	23.68373744647867	25.519879955713048	26.24939503756319	24.546987560245093
66-67	23.8196380628181	25.7428644597653	25.963565171305664	24.473932306110935
68-69	23.70913021847678	25.682648515693383	26.113412801116887	24.494808464712943
70-71	23.637009384011222	25.494546556963275	26.08483681972969	24.78360723929581
72-73	23.6111953102861	25.937301299737626	25.820795097117287	24.630708292858987
74-75	23.553897056555893	25.85231232845329	25.936989765573028	24.656800849417788
76-77	23.45044949575231	25.38545364630248	26.095851216022893	25.06824564192232
78-79	23.334135541433167	25.465681801953334	26.27746350614306	24.92271915047044
80-81	23.652620193104344	25.676827690370235	25.819491227241347	24.85106088928407
82-83	23.624871602522116	25.721893838348436	25.976442003460587	24.676792555668865
84-85	23.50812905035977	25.75251148521695	25.87013505208729	24.86922441233599
86-87	23.14870521686692	26.02958446330772	25.818571665355954	25.00313865446941
88-89	23.13098162196997	25.62612707466534	25.974884254024964	25.268007049339726
90-91	23.27734759793851	25.42879278007259	26.09506433372049	25.19879528826841
92-93	23.418959024190062	25.29413485993427	26.065450030213782	25.221456085661885
94-95	23.220831245458655	25.376522210405618	26.044025436480332	25.3586211076554
96-97	22.91790337475281	25.213180722454247	26.15527256561667	25.71364333717627
98-99	22.60075823485407	25.210850813645408	26.147897024109668	26.040493927390855
100-101	22.69823480608132	25.356006643636753	26.112586280682198	25.833172269599725
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	68.0
1	54.0
2	75.5
3	111.0
4	93.0
5	90.0
6	165.5
7	364.0
8	563.0
9	635.5
10	513.5
11	383.5
12	386.0
13	400.0
14	439.0
15	473.0
16	549.5
17	626.5
18	685.5
19	768.0
20	851.5
21	971.0
22	1086.0
23	1254.5
24	1500.5
25	1860.5
26	2409.0
27	3148.5
28	4262.5
29	6182.5
30	9389.5
31	14164.5
32	21130.0
33	27292.0
34	32769.5
35	39488.5
36	48047.5
37	56915.0
38	63948.5
39	70144.5
40	75474.0
41	80488.5
42	86520.5
43	93553.5
44	99969.0
45	106679.5
46	112797.5
47	118258.5
48	124454.5
49	129114.0
50	130588.5
51	131402.5
52	130902.0
53	128103.5
54	124344.5
55	117875.0
56	108936.0
57	97869.5
58	86499.5
59	73801.0
60	59947.0
61	49013.5
62	40228.5
63	30945.0
64	22075.5
65	15945.5
66	11833.0
67	9079.0
68	7007.0
69	5320.0
70	3947.5
71	2906.0
72	2160.5
73	1654.0
74	1283.0
75	949.0
76	693.0
77	431.5
78	242.5
79	141.5
80	88.0
81	62.0
82	42.0
83	23.0
84	18.0
85	12.5
86	10.5
87	9.5
88	4.5
89	1.5
90	1.0
91	1.0
92	1.5
93	3.0
94	2.0
95	0.5
96	1.5
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15680365785935169
2	3.5236777047045325E-4
3	0.005673121104574298
4	0.004228413245645439
5	0.02614568856890763
6	0.0013389975277877224
7	2.818942163763626E-4
8	0.0015504181900699943
9	0.0
10-11	0.10703171028040018
12-13	4.052229360410213E-4
14-15	0.00523266139148623
16-17	2.466574393293173E-4
18-19	4.052229360410213E-4
20-21	8.104458720820426E-4
22-23	2.2903905080579463E-4
24-25	0.0015327998015464716
26-27	0.0033651122079928287
28-29	0.007364486402832473
30-31	0.014781827971235513
32-33	0.014852301525329605
34-35	0.010923400884584052
36-37	8.104458720820426E-4
38-39	2.9951260489988527E-4
40-41	0.005479318830815548
42-43	0.008721102319143718
44-45	0.00852730004538497
46-47	0.01032437567478428
48-49	0.011998122584518933
50-51	0.006272146314374069
52-53	0.0020613514572521514
54-55	9.690113687937464E-4
56-57	0.007064973797932588
58-59	0.009883915961696214
60-61	0.008086840332296903
62-63	0.01846407117265175
64-65	0.03491964605362192
66-67	0.03772096982886202
68-69	0.02739659415407774
70-71	0.04242507956464257
72-73	0.026639003447566263
74-75	0.02489478298373752
76-77	0.246446018667035
78-79	0.07123114480060212
80-81	0.05461700442292026
82-83	0.01985592386601004
84-85	0.03268211071113454
86-87	0.08224263762780379
88-89	0.2680813997739208
90-91	0.3247949924311403
92-93	0.052502797800097534
94-95	0.004580781016115892
96-97	0.009496311414178716
98-99	0.001303760750740677
100-101	0.0014270894704053357
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2837944.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.55874513376167
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.89656331395133	45.61524997897605
2	12.204693465647159	14.293830681810368
3	3.998350637420344	7.024151877963343
4	1.8314066979643477	4.289795122494331
5	1.013188038785623	2.9665510067911556
6	0.6658945797883018	2.3396370590265896
7	0.4440631298703141	1.820264575176336
8	0.31866567217418784	1.4928529503781691
9	0.2526316853133226	1.3314415025677986
>10	1.2891024729210407	13.437909601935683
>50	0.06313322872160991	2.5113987888273845
>100	0.020679090065657244	2.0510514906540482
>500	0.0011456207466556028	0.4665662639017107
>1k	4.823666300759914E-4	0.3592990994970068
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.047355409409065E-5
2	0.0	0.0	0.0	0.0	7.047355409409065E-5
3	0.0	0.0	0.0	0.0	7.047355409409065E-5
4	0.0	0.0	0.0	0.0	7.047355409409065E-5
5	0.0	0.0	0.0	0.0	7.047355409409065E-5
6	0.0	0.0	0.0	0.0	1.0571033114113597E-4
7	0.0	0.0	0.0	0.0	1.0571033114113597E-4
8	0.0	0.0	0.0	0.0	1.409471081881813E-4
9	0.0	0.0	0.0	0.0	1.7618388523522662E-4
10-11	0.0	0.0	0.0	0.0	1.7618388523522662E-4
12-13	0.0	0.0	0.0	3.5236777047045326E-5	1.9380227375874928E-4
14-15	0.0	0.0	0.0	3.5236777047045326E-5	2.818942163763626E-4
16-17	0.0	0.0	0.0	5.285516557056799E-5	3.5236777047045325E-4
18-19	0.0	0.0	0.0	7.047355409409065E-5	3.5236777047045325E-4
20-21	0.0	0.0	0.0	1.2332871966465865E-4	3.5236777047045325E-4
22-23	0.0	0.0	0.0	2.9951260489988527E-4	4.404597130880666E-4
24-25	0.0	0.0	0.0	5.461700442292026E-4	5.285516557056799E-4
26-27	0.0	0.0	0.0	0.001250905585170109	5.285516557056799E-4
28-29	0.0	0.0	0.0	0.003294638653898738	5.285516557056799E-4
30-31	0.0	0.0	0.0	0.01323140978116552	5.461700442292026E-4
32-33	0.0	0.0	0.0	0.03581818386832157	5.637884327527252E-4
34-35	0.0	0.0	0.0	0.0668617844467685	5.637884327527252E-4
36-37	0.0	1.7618388523522663E-5	0.0	0.1131981462636331	5.637884327527252E-4
38-39	0.0	5.285516557056799E-5	0.0	0.19041954316223295	5.637884327527252E-4
40-41	0.0	7.047355409409065E-5	0.0	0.2779124605700465	5.637884327527252E-4
42-43	0.0	7.047355409409065E-5	0.0	0.3690876211792763	5.814068212762479E-4
44-45	0.0	7.047355409409065E-5	0.0	0.4817219790101567	6.342619868468159E-4
46-47	0.0	7.047355409409065E-5	0.0	0.6101424129581133	6.694987638938612E-4
48-49	0.0	7.047355409409065E-5	0.0	0.74011326509614	6.694987638938612E-4
50-51	0.0	7.047355409409065E-5	0.0	0.8864516001725193	6.871171524173838E-4
52-53	0.0	7.047355409409065E-5	0.0	1.036313612953603	7.752090950349971E-4
54-55	0.0	7.047355409409065E-5	0.0	1.2070710345235847	8.104458720820425E-4
56-57	0.0	7.047355409409065E-5	0.0	1.4053483789673087	8.104458720820425E-4
58-59	0.0	7.047355409409065E-5	0.0	1.6218959923099256	8.104458720820425E-4
60-61	0.0	7.047355409409065E-5	0.0	1.849472716868268	8.104458720820425E-4
62-63	0.0	7.047355409409065E-5	0.0	2.0921836371683162	8.104458720820425E-4
64-65	0.0	7.047355409409065E-5	0.0	2.3548914284425626	8.104458720820425E-4
66-67	0.0	7.047355409409065E-5	0.0	2.657346304225876	8.280642606055651E-4
68-69	0.0	7.047355409409065E-5	0.0	2.9869511167239384	8.456826491290878E-4
70-71	0.0	7.047355409409065E-5	0.0	3.3400588595123795	8.809194261761331E-4
72-73	0.0	7.047355409409065E-5	0.0	3.7061689730311804	9.513929802702238E-4
74-75	0.0	7.047355409409065E-5	0.0	4.085316694057388	9.513929802702238E-4
76-77	0.0	8.809194261761331E-5	0.0	4.520578982531015	9.513929802702238E-4
78-79	0.0	1.0571033114113597E-4	0.0	5.005718928914735	9.513929802702238E-4
80-81	0.0	1.0571033114113597E-4	0.0	5.499298083401223	9.513929802702238E-4
82-83	0.0	1.0571033114113597E-4	0.0	6.011975571047209	0.0010923400884584051
84-85	0.0	1.0571033114113597E-4	0.0	6.559713651855005	0.0010923400884584051
86-87	0.0	1.0571033114113597E-4	0.0	7.144168454345822	0.0010923400884584051
88-89	0.0	1.0571033114113597E-4	0.0	7.777355719492704	0.0010923400884584051
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2400	0.0	52.87256	1
GTATCAA	4555	0.0	40.17008	1
TCAACGC	5705	0.0	31.690802	4
CAACGCA	5860	0.0	31.07586	5
TATCAAC	5820	0.0	30.820005	2
ATCAACG	5875	0.0	30.773792	3
AACGCAG	6145	0.0	29.630922	6
GTACATG	9760	0.0	28.242847	1
TACATGG	10245	0.0	26.308752	2
ACATGGG	10530	0.0	24.560207	3
ACGCAGA	7620	0.0	23.39616	7
CATGGGG	8710	0.0	23.099981	4
CGCAGAG	8055	0.0	21.778559	8
TAAGGCG	765	0.0	20.510418	5
TATACCG	235	1.12160706E-7	20.232714	5
ATGGGGG	5880	0.0	19.8112	5
GTATAGC	800	0.0	19.010359	1
TATATAC	900	0.0	18.981209	3
GCAGAGT	9130	0.0	18.224918	9
GAGTACA	7465	0.0	17.889854	1
>>END_MODULE