FastQCFastQC Report
Mon 15 Aug 2016
SRR2921405_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921405_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2475324
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24350.057.682321
GTATCAA42700.040.2282031
TCAACGC50800.033.5377044
CAACGCA51700.032.6765175
ATCAACG52450.032.3915253
TATCAAC53750.031.7853262
AACGCAG55250.030.6541546
ACGCAGA66000.025.089757
GTACATG57150.024.2446861
CGCAGAG68000.024.004398
TACATGG60750.022.107662
ACATGGG60950.021.4117833
GTGGTAT12550.020.039281
GCAGAGT78150.019.9152629
GAGTACT40900.019.49068612-13
GTAGGAC5650.019.3184933
GTATTAG7050.018.8459851
CAGAGTA71700.018.66255810-11
CATGGGG50800.018.123444
AGAGTAC65600.017.32378810-11
Produced by FastQC (version 0.11.5)