##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921405_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2475324
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.519443515273153	31.0	31.0	34.0	27.0	34.0
2	30.576911951728338	33.0	31.0	34.0	27.0	34.0
3	30.619961265676736	33.0	31.0	34.0	26.0	34.0
4	34.09004437398902	37.0	35.0	37.0	30.0	37.0
5	34.085205411493604	37.0	35.0	37.0	30.0	37.0
6	34.13564446512861	37.0	35.0	37.0	30.0	37.0
7	34.058911479870915	37.0	35.0	37.0	30.0	37.0
8	34.0282524631119	37.0	35.0	37.0	30.0	37.0
9	35.602209650130646	39.0	37.0	39.0	30.0	39.0
10-11	35.49925848090997	39.0	37.0	39.0	30.0	39.0
12-13	35.39605986933428	39.0	36.0	39.0	30.0	39.0
14-15	36.5377572794511	40.0	37.0	41.0	29.5	41.0
16-17	36.49026066890637	40.0	37.0	41.0	29.0	41.0
18-19	36.45783259080427	40.0	37.0	41.0	29.0	41.0
20-21	36.39976726279065	40.0	37.0	41.0	29.0	41.0
22-23	36.28376770071311	40.0	37.0	41.0	28.0	41.0
24-25	36.19017975020644	40.0	36.5	41.0	27.0	41.0
26-27	36.042951548968944	40.0	36.0	41.0	27.0	41.0
28-29	35.89990825443457	40.0	36.0	41.0	27.0	41.0
30-31	35.749532586441205	40.0	36.0	41.0	27.0	41.0
32-33	35.55382689296432	39.5	35.5	41.0	26.0	41.0
34-35	35.38143168328671	39.0	35.0	41.0	25.0	41.0
36-37	35.20116093893163	39.0	35.0	41.0	25.0	41.0
38-39	35.40965283736594	39.5	35.0	41.0	25.0	41.0
40-41	35.51442094045063	40.0	35.0	41.0	25.0	41.0
42-43	35.33627113056715	40.0	35.0	41.0	24.0	41.0
44-45	35.1959957565151	40.0	35.0	41.0	24.0	41.0
46-47	35.01892519928704	40.0	35.0	41.0	23.0	41.0
48-49	34.85298086230328	39.5	35.0	41.0	22.5	41.0
50-51	33.13400912365412	37.0	32.5	39.5	18.5	40.0
52-53	33.286944254570315	37.5	33.5	39.5	18.5	40.0
54-55	34.14884920115508	39.0	34.0	40.5	19.5	41.0
56-57	34.162840702873645	39.0	34.0	41.0	19.0	41.0
58-59	33.98880368792126	38.5	34.0	41.0	18.0	41.0
60-61	33.75418329075305	38.0	34.0	40.5	16.0	41.0
62-63	33.469906363772985	37.5	34.0	40.0	13.5	41.0
64-65	33.18155340472601	37.0	33.5	40.0	12.0	41.0
66-67	32.8413688470681	36.5	33.0	39.5	8.0	41.0
68-69	32.4851011423151	36.0	33.0	39.0	7.0	41.0
70-71	32.104720836545035	35.0	32.5	39.0	2.0	41.0
72-73	31.748312746129393	35.0	32.0	38.0	2.0	40.0
74-75	31.35159074125246	35.0	32.0	37.0	2.0	39.5
76-77	30.97544018480005	35.0	32.0	37.0	2.0	39.0
78-79	30.62704639877446	35.0	31.5	36.0	2.0	39.0
80-81	30.2721108428634	35.0	31.0	36.0	2.0	37.5
82-83	29.966994825727866	35.0	31.0	35.0	2.0	37.0
84-85	29.72467967829666	35.0	31.0	35.0	2.0	36.5
86-87	29.496045770169886	35.0	31.0	35.0	2.0	36.0
88-89	29.283735987692925	35.0	31.0	35.0	2.0	36.0
90-91	29.021275598669106	35.0	30.0	35.0	2.0	36.0
92-93	28.82709677601801	34.0	30.0	35.0	2.0	35.0
94-95	28.619364374118298	34.0	30.0	35.0	2.0	35.0
96-97	28.367509667421317	34.0	30.0	35.0	2.0	35.0
98-99	28.13034778477484	34.0	29.5	35.0	2.0	35.0
100-101	27.077241403549596	33.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	78061.0
3	13276.0
4	9273.0
5	7016.0
6	6612.0
7	6776.0
8	7363.0
9	8092.0
10	8688.0
11	9448.0
12	9635.0
13	9977.0
14	10884.0
15	12132.0
16	12798.0
17	14175.0
18	12499.0
19	10739.0
20	10763.0
21	11062.0
22	12306.0
23	13862.0
24	15926.0
25	18574.0
26	22266.0
27	26699.0
28	31045.0
29	36594.0
30	43724.0
31	52998.0
32	65862.0
33	85318.0
34	116933.0
35	172707.0
36	287927.0
37	519353.0
38	570279.0
39	113658.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.78523606038412	18.397285721907757	13.095971427970904	24.721506789737223
2	16.42583510216225	19.68422614223365	38.92117537822487	24.968763377379226
3	19.561741329246132	23.141600217294297	29.55489429537797	27.7417641580816
4	13.768953380877717	15.879872217903804	35.926244361633195	34.42493003958529
5	14.60651177679821	36.49041147050526	34.28496480743316	14.618111945263374
6	33.66794942453682	34.63951325238113	16.992810952952794	14.699726370129252
7	28.9374150237075	31.686717536278437	20.83621722415155	18.539650215862512
8	26.381393897933613	34.45307319659237	19.982293525822882	19.18323937965113
9	26.372425263130673	15.890312653546768	19.887579521063383	37.849682562259176
10-11	25.567008726288392	25.243806125312577	27.820274543405866	21.368910604993165
12-13	26.129864252022276	22.987485970082304	27.40248260088155	23.48016717701387
14-15	23.362847739373258	24.752693743611466	24.903371206135095	26.98108731088018
16-17	23.312529032839464	27.646072436467207	25.98592441470099	23.055474115992343
18-19	23.287799654507065	26.737718332339956	27.473840527735405	22.50064148541757
20-21	23.747998207428342	25.984388947640014	27.50526638641234	22.762346458519303
22-23	23.67475362817923	25.880904051806997	27.119715161759437	23.324627158254334
24-25	23.41892556575707	26.10612262054254	27.193421362666925	23.281530451033465
26-27	23.206588385340044	26.349823492197427	27.230937995863062	23.212650126599467
28-29	23.31065866840742	26.46975691054003	27.18274713172459	23.03683728932796
30-31	23.64015134603547	26.070968757253816	27.02745727710874	23.261422619601973
32-33	23.300155954555006	26.156447468364657	26.92218029316224	23.621216283918095
34-35	23.70564946710878	26.29158294893545	26.86562430431626	23.13714327963951
36-37	23.711779808063284	26.14072262094013	26.882997784102503	23.26449978689409
38-39	23.396551988836304	26.29894318199264	27.06258893342911	23.24191589574194
40-41	23.654218261758896	26.11807686799299	27.057721795855638	23.169983074392476
42-43	23.563520890616036	26.101558764309118	26.764273489669982	23.57064685540486
44-45	23.55937517013501	26.214220038377402	26.795831310265743	23.43057348122185
46-47	23.722660830964198	26.337878105226427	26.480005958763897	23.45945510504548
48-49	23.44108973083603	26.433655828688114	26.564951870599216	23.560302569876637
50-51	23.504402898618775	26.478049463694	26.68534231858738	23.332205319099845
52-53	23.79495259072087	26.231825565913734	26.617305530468606	23.35591631289679
54-55	23.358583609679005	26.24134935490557	26.744438813181688	23.655628222233737
56-57	23.45009139435602	26.467744775947192	26.578592061342516	23.503571768354274
58-59	23.386608527418147	26.31372483135212	26.78922005706914	23.51044658416059
60-61	23.316198630303365	26.2638479763048	26.968554304471677	23.451399088920162
62-63	23.368133529762545	26.22137718255245	27.012518921291072	23.397970366393924
64-65	23.2444123716766	26.132459406638052	26.838085039841868	23.785043181843484
66-67	23.178828589500316	26.302218848829856	26.688364326375712	23.83058823529412
68-69	23.324940443541287	26.132523999684253	26.853698572466598	23.68883698430786
70-71	23.19000946867173	25.961867499710113	26.914218771849125	23.933904259769033
72-73	23.165576359672592	26.057149842262355	26.837317723364517	23.939956074700532
74-75	23.149824362358558	26.221929802015286	26.743489709558872	23.884756126067288
76-77	23.163645260919168	26.021691303180194	26.776723994347172	24.037939441553473
78-79	23.080067029886926	26.011248032849345	26.854202708321612	24.054482228942124
80-81	23.091255061531992	26.069048396556465	26.810060121920138	24.029636419991405
82-83	23.05406544570559	26.04140897067499	26.917754169057208	23.986771414562213
84-85	23.091534171641324	26.01320965668942	26.902909003089352	23.992347168579908
86-87	23.034717372778317	26.072236933936182	26.830022370938877	24.063023322346623
88-89	22.94675074720484	25.995480124411984	26.798241937658762	24.259527190724413
90-91	22.872504658187346	25.842021901325996	26.986165441726545	24.29930799876011
92-93	22.890195296325803	25.67026496763959	27.202568180598735	24.236971555435872
94-95	22.851461370155384	25.519521216115926	27.273691894754215	24.355325518974475
96-97	22.899324385073776	25.39705843044471	27.22577899024688	24.477838194234632
98-99	22.79192524575559	25.44487076951112	27.145934782388615	24.617269202344673
100-101	22.813646327449085	25.28276288012636	27.329185503181748	24.574405289242808
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	89.0
1	67.0
2	71.0
3	118.0
4	119.5
5	126.5
6	219.0
7	437.0
8	683.5
9	787.5
10	719.0
11	624.0
12	646.0
13	712.0
14	803.0
15	933.0
16	1045.0
17	1180.0
18	1317.5
19	1510.0
20	1697.0
21	1867.5
22	2152.5
23	2532.0
24	3039.0
25	3853.0
26	5137.5
27	7039.5
28	9631.0
29	12998.0
30	17275.5
31	22727.0
32	29692.0
33	34474.0
34	37749.5
35	43032.0
36	49569.5
37	56058.0
38	61292.5
39	66120.5
40	70508.0
41	74557.5
42	78799.0
43	83493.5
44	88358.5
45	93956.0
46	97860.0
47	100091.5
48	102843.5
49	104470.0
50	103919.5
51	101720.5
52	99964.0
53	97969.0
54	95194.0
55	91160.0
56	84197.5
57	75189.5
58	67401.0
59	59333.0
60	49377.0
61	40494.0
62	33133.5
63	25687.0
64	18675.5
65	13644.5
66	10209.5
67	7869.5
68	6118.0
69	4678.0
70	3632.5
71	2733.0
72	1967.0
73	1461.5
74	1137.0
75	904.0
76	709.5
77	470.0
78	298.0
79	215.5
80	153.5
81	113.5
82	85.0
83	73.0
84	58.0
85	42.5
86	40.5
87	39.5
88	37.5
89	31.5
90	24.5
91	24.5
92	21.5
93	16.5
94	13.0
95	12.5
96	12.5
97	7.0
98	5.0
99	6.0
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05235678238485144
2	0.05918417144583901
3	0.050902427318605566
4	0.05756822137223249
5	0.049367274748679364
6	0.01809864082439309
7	0.014907139429020201
8	0.021128547212405327
9	0.020522565934802877
10-11	0.022643500406411443
12-13	0.028339724415874446
14-15	0.04706454589379006
16-17	0.030521257015243254
18-19	0.024198852352257726
20-21	0.026945967477388817
22-23	0.04920567974131871
24-25	0.043347860724495056
26-27	0.03181401707412848
28-29	0.021633531610407365
30-31	0.013472983738694409
32-33	0.009857295448999806
34-35	0.009736099193479317
36-37	0.0011715638033647313
38-39	0.0063830027907457775
40-41	0.003252099523133133
42-43	0.22150635633961455
44-45	0.19631773456727283
46-47	0.20251894297473785
48-49	0.200074818488408
50-51	0.1981962765278404
52-53	0.20308452550050013
54-55	0.20150897417873376
56-57	0.212457035927418
58-59	0.20855855637484225
60-61	0.21362859973078274
62-63	0.2110026808611721
64-65	0.21195205152941596
66-67	0.2024583448469776
68-69	0.20114538541217228
70-71	0.1835921277376214
72-73	0.19254045127021754
74-75	0.19207586562405568
76-77	0.1899751305283672
78-79	0.2173856836519179
80-81	0.22593001966611242
82-83	0.22304150890954072
84-85	0.21768867429071914
86-87	0.2353429288448704
88-89	0.23378757689902413
90-91	0.2321918262013377
92-93	0.2372012714295179
94-95	0.24851292194476357
96-97	0.24909870384644595
98-99	0.23831223710512242
100-101	0.24891690946316522
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2475324.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.3750913535487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.10100495056204	52.208846025570374
2	10.949022719693883	14.096886756247477
3	3.2818253674851876	6.338034235147575
4	1.4677889600447918	3.7795619356245487
5	0.8201052044165572	2.63971737269183
6	0.53167995601355	2.0536167443533824
7	0.3531727429000854	1.5914869311443456
8	0.2656372113371957	1.3680335797387115
9	0.20586916013349568	1.1927561391424917
>10	0.9720786181884447	10.805869618975608
>50	0.03787308117736977	1.6424504543347496
>100	0.012495189471478723	1.4213538093439382
>500	0.001069402425769163	0.49753153504728004
>1k	3.77436150267466E-4	0.3638548626376578
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.039875184016315E-5	0.0
5	0.0	0.0	0.0	8.07975036803263E-5	0.0
6	0.0	0.0	0.0	8.07975036803263E-5	0.0
7	0.0	0.0	0.0	8.07975036803263E-5	0.0
8	0.0	0.0	0.0	8.07975036803263E-5	0.0
9	0.0	0.0	0.0	8.07975036803263E-5	4.039875184016315E-5
10-11	0.0	0.0	0.0	1.2119625552048943E-4	4.039875184016315E-5
12-13	0.0	0.0	0.0	1.615950073606526E-4	1.0099687960040787E-4
14-15	0.0	0.0	0.0	2.0199375920081574E-4	2.221931351208973E-4
16-17	0.0	0.0	0.0	2.0199375920081574E-4	3.231900147213052E-4
18-19	0.0	0.0	0.0	2.6259188696106043E-4	3.635887665614683E-4
20-21	0.0	0.0	0.0	2.8279126288114205E-4	3.837881424815499E-4
22-23	0.0	0.0	0.0	4.443862702417946E-4	4.2418689432171305E-4
24-25	0.0	0.0	0.0	7.271775331229366E-4	4.443862702417946E-4
26-27	0.0	0.0	0.0	0.0011513644274446498	4.443862702417946E-4
28-29	0.0	0.0	0.0	0.0037974826729753356	4.645856461618762E-4
30-31	0.0	0.0	0.0	0.01516973131598126	5.049843980020393E-4
32-33	0.0	0.0	0.0	0.042701480695052446	5.251837739221209E-4
34-35	0.0	0.0	0.0	0.07976733550840212	5.251837739221209E-4
36-37	0.0	0.0	0.0	0.12994258529388475	5.655825257622841E-4
38-39	0.0	0.0	0.0	0.1980750802723199	5.655825257622841E-4
40-41	0.0	0.0	0.0	0.2730753630635828	5.655825257622841E-4
42-43	0.0	0.0	0.0	0.36007407515137413	5.655825257622841E-4
44-45	0.0	0.0	0.0	0.48175511569394547	6.059812776024472E-4
46-47	0.0	0.0	0.0	0.6214943983090699	6.463800294426104E-4
48-49	0.0	0.0	0.0	0.7524671517748788	6.463800294426104E-4
50-51	0.0	0.0	0.0	0.8868737991471016	6.463800294426104E-4
52-53	0.0	0.0	0.0	1.0325920970345701	6.463800294426104E-4
54-55	0.0	0.0	0.0	1.219496922423085	6.463800294426104E-4
56-57	0.0	0.0	0.0	1.4498910041675352	6.463800294426104E-4
58-59	0.0	0.0	0.0	1.6744878650229222	6.665794053626919E-4
60-61	0.0	2.0199375920081574E-5	0.0	1.8872276922132212	7.473769090430182E-4
62-63	0.0	4.039875184016315E-5	0.0	2.118894334640637	8.483737886434261E-4
64-65	0.0	4.039875184016315E-5	0.0	2.395989373512316	8.483737886434261E-4
66-67	0.0	4.039875184016315E-5	0.0	2.7414996986253115	8.483737886434261E-4
68-69	0.0	4.039875184016315E-5	0.0	3.0988468580274744	8.483737886434261E-4
70-71	0.0	4.039875184016315E-5	0.0	3.4468417063786396	8.887725404835892E-4
72-73	0.0	4.039875184016315E-5	0.0	3.8056230214711286	0.0010099687960040786
74-75	0.0	4.039875184016315E-5	0.0	4.218417467773915	0.0010503675478442417
76-77	0.0	4.039875184016315E-5	0.0	4.713786962837997	0.0010503675478442417
78-79	0.0	4.039875184016315E-5	0.0	5.253655683054016	0.0010503675478442417
80-81	0.0	4.039875184016315E-5	0.0	5.772921039831553	0.0010503675478442417
82-83	0.0	4.039875184016315E-5	0.0	6.293115567901414	0.0010503675478442417
84-85	0.0	4.039875184016315E-5	0.0	6.855122804125844	0.0010503675478442417
86-87	0.0	4.039875184016315E-5	0.0	7.516329175493794	0.0010503675478442417
88-89	0.0	4.039875184016315E-5	0.0	8.24324411672977	0.0010705669237643233
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2435	0.0	57.68232	1
GTATCAA	4270	0.0	40.228203	1
TCAACGC	5080	0.0	33.537704	4
CAACGCA	5170	0.0	32.676517	5
ATCAACG	5245	0.0	32.391525	3
TATCAAC	5375	0.0	31.785326	2
AACGCAG	5525	0.0	30.654154	6
ACGCAGA	6600	0.0	25.08975	7
GTACATG	5715	0.0	24.244686	1
CGCAGAG	6800	0.0	24.00439	8
TACATGG	6075	0.0	22.10766	2
ACATGGG	6095	0.0	21.411783	3
GTGGTAT	1255	0.0	20.03928	1
GCAGAGT	7815	0.0	19.915262	9
GAGTACT	4090	0.0	19.490686	12-13
GTAGGAC	565	0.0	19.318493	3
GTATTAG	705	0.0	18.845985	1
CAGAGTA	7170	0.0	18.662558	10-11
CATGGGG	5080	0.0	18.12344	4
AGAGTAC	6560	0.0	17.323788	10-11
>>END_MODULE