FastQCFastQC Report
Mon 15 Aug 2016
SRR2921405_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921405_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2475324
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25100.056.7987141
GTATCAA44900.041.700461
TCAACGC57850.032.0782854
CAACGCA58750.031.402265
ATCAACG59800.030.9528873
AACGCAG61900.029.8792466
TATCAAC62200.029.5296522
ACGCAGA70800.025.518337
CGCAGAG75550.023.788078
GTACATG59600.021.2890341
GCAGAGT84100.020.352069
GTGGTAT11950.019.485841
TACATGG63800.019.341512
AGAGTAC72550.018.77539610-11
TGGTATC12150.018.7500612
ACATGGG63350.018.5798763
CAGAGTA79300.018.3463710-11
GAGTACT47050.018.00123412-13
GTATAGA7400.017.981141
GTCCTAA7250.017.6976951
Produced by FastQC (version 0.11.5)