##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921405_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2475324
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3902305314375	33.0	31.0	34.0	29.0	34.0
2	31.52189006368459	34.0	31.0	34.0	28.0	34.0
3	31.59116665131514	34.0	31.0	34.0	28.0	34.0
4	35.08829308809675	37.0	35.0	37.0	32.0	37.0
5	34.827154748226896	37.0	35.0	37.0	32.0	37.0
6	34.78829801674448	37.0	35.0	37.0	32.0	37.0
7	34.65516877790544	37.0	35.0	37.0	32.0	37.0
8	34.618174428882845	37.0	35.0	37.0	32.0	37.0
9	36.23872066848622	39.0	37.0	39.0	32.0	39.0
10-11	36.09045139141381	39.0	37.0	39.0	32.0	39.0
12-13	36.02890651890419	39.0	37.0	39.0	31.0	39.0
14-15	37.26764738676634	40.0	38.0	41.0	31.0	41.0
16-17	37.24732216873427	40.0	38.0	41.0	31.0	41.0
18-19	37.21923978436762	40.0	38.0	41.0	31.0	41.0
20-21	37.18656810179193	40.0	38.0	41.0	31.0	41.0
22-23	37.077267056757016	40.0	38.0	41.0	31.0	41.0
24-25	37.01381233325415	40.0	37.0	41.0	31.0	41.0
26-27	36.872780694567666	40.0	37.0	41.0	30.0	41.0
28-29	36.716117566831656	40.0	37.0	41.0	30.0	41.0
30-31	36.566040243620634	40.0	37.0	41.0	30.0	41.0
32-33	36.49673759879515	40.0	36.5	41.0	30.0	41.0
34-35	36.609076023987164	40.0	37.0	41.0	30.0	41.0
36-37	36.56943495073776	40.0	37.0	41.0	30.0	41.0
38-39	36.46673061789083	40.0	37.0	41.0	30.0	41.0
40-41	36.33015193162592	40.0	36.5	41.0	29.5	41.0
42-43	36.204415260386114	40.0	36.0	41.0	29.0	41.0
44-45	36.04391122131891	40.0	36.0	41.0	28.0	41.0
46-47	35.861966958668845	40.0	36.0	41.0	27.5	41.0
48-49	35.67765674311727	40.0	35.0	41.0	27.0	41.0
50-51	35.51584944031569	40.0	35.0	41.0	26.0	41.0
52-53	35.34039422717996	39.0	35.0	41.0	26.0	41.0
54-55	35.13070369777856	39.0	35.0	41.0	26.0	41.0
56-57	34.862577989790424	39.0	35.0	41.0	25.0	41.0
58-59	34.63980291064927	38.5	35.0	40.5	24.0	41.0
60-61	34.37209048189247	38.0	34.0	40.0	23.5	41.0
62-63	34.08388154439581	37.5	34.0	40.0	23.0	41.0
64-65	33.77326160130957	37.0	34.0	40.0	22.5	41.0
66-67	33.441293341800915	36.5	34.0	39.5	22.0	41.0
68-69	33.0813600562997	36.0	33.5	39.0	21.5	41.0
70-71	32.70665739111324	35.5	33.0	39.0	20.0	40.0
72-73	32.3159636879859	35.0	33.0	37.5	19.0	40.0
74-75	31.913088145228667	35.0	33.0	37.0	17.5	39.0
76-77	29.15461410304267	33.0	28.5	34.5	9.0	36.5
78-79	30.802936706467516	34.5	31.5	36.0	11.0	38.0
80-81	30.994390229319475	35.0	32.0	36.0	11.0	37.0
82-83	30.828118662445803	35.0	32.0	35.0	8.5	37.0
84-85	30.58745340003975	35.0	32.0	35.0	6.5	36.5
86-87	30.332951363134683	35.0	32.0	35.0	2.0	36.0
88-89	30.04353531093303	35.0	31.0	35.0	2.0	36.0
90-91	29.849963883515855	34.0	31.0	35.0	2.0	35.5
92-93	29.69706410150752	34.0	31.0	35.0	2.0	35.0
94-95	29.589507272583305	34.0	31.0	35.0	2.0	35.0
96-97	29.390947003301385	34.0	31.0	35.0	2.0	35.0
98-99	29.112907239617925	34.0	31.0	35.0	2.0	35.0
100-101	28.032518369312463	33.0	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	46515.0
3	15168.0
4	8193.0
5	5880.0
6	5191.0
7	5319.0
8	5696.0
9	6043.0
10	6904.0
11	7577.0
12	8097.0
13	9056.0
14	10062.0
15	11447.0
16	12935.0
17	14256.0
18	12744.0
19	10752.0
20	9650.0
21	9402.0
22	10103.0
23	11095.0
24	12756.0
25	15427.0
26	18508.0
27	22408.0
28	26733.0
29	32220.0
30	39195.0
31	48718.0
32	61103.0
33	79993.0
34	113743.0
35	171188.0
36	299732.0
37	575985.0
38	626323.0
39	89204.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.334876403967016	20.049452170137798	13.818446363493278	25.797225062401907
2	15.331382066524867	21.02079937300909	37.57723764450181	26.070580915964232
3	18.082158795519316	24.25820897090206	29.41779878294638	28.241833450632242
4	13.341475367250036	17.062516841930478	34.96128224510054	34.63472554571895
5	14.261318083070398	36.894776741840374	34.26278093156779	14.58112424352144
6	33.58631368998635	35.26090467747024	16.700016280914042	14.452765351629365
7	28.600140587883587	31.94871774154453	20.849667921723253	18.601473748848633
8	26.215133293795784	35.155885142876535	19.625667196168198	19.003314367159486
9	26.09682611246043	15.972292920037942	19.791994906525368	38.13888606097626
10-11	25.448995789037344	25.610599695678328	27.698226138299542	21.24217837698479
12-13	25.93466778813874	23.217057363942732	27.43098058512078	23.41729426279775
14-15	23.142789038417252	24.934429276136377	24.894270742852065	27.02851094259431
16-17	23.09704478732253	27.914278592554258	25.965258647262313	23.023417972860894
18-19	23.09847274260959	26.908122365617853	27.544403863824446	22.44900102794811
20-21	23.572510399720116	26.079451962928236	27.58920408660421	22.758833550747443
22-23	23.43045828684938	25.940076753821177	27.25847381536866	23.370991143960783
24-25	23.21904346368196	26.27467140147498	27.25635633876503	23.249928796078027
26-27	23.005536780910326	26.47851846165966	27.328669859590793	23.18727489783922
28-29	23.124778298414576	26.636895022329742	27.229944238263652	23.00838244099203
30-31	23.39748109365675	26.23659424403516	27.113496242166047	23.25242842014204
32-33	23.147973248025473	26.299199077155873	26.953210307651748	23.59961736716691
34-35	23.551845328149806	26.41970036207781	26.95450401319908	23.0739502965733
36-37	23.4792397552143	26.28472901150079	26.95648379918782	23.27954743409709
38-39	23.26476510819508	26.406540082385295	27.13478921214445	23.19390559727518
40-41	23.5046497140036	26.209980098626133	27.08673540707319	23.198634780297077
42-43	23.441637107511692	26.202067252052068	26.822642848037965	23.53365279239827
44-45	23.40335401106853	26.313407883073424	26.7682938161532	23.514944289704847
46-47	23.533057492292222	26.428156149031736	26.533020726056396	23.505765632619653
48-49	23.315241962821446	26.552517162425165	26.63350611245363	23.498734762299755
50-51	23.3311166666936	26.611715958545375	26.647147610605742	23.410019764155287
52-53	23.694235427982292	26.368413961471482	26.505125574504902	23.432225036041327
54-55	23.231622367968953	26.373647461104188	26.672621504032744	23.722108666894115
56-57	23.306774920379304	26.52315615918564	26.48952214653279	23.680546773902268
58-59	23.21399964970978	26.444831007189435	26.65027804033832	23.690891302762466
60-61	23.149848583618994	26.456343579737375	26.813577557690436	23.58023027895319
62-63	23.272070856246994	26.3573036159475	26.762961286855468	23.60766424095003
64-65	23.11208532922093	26.24553286915181	26.679081481517404	23.96330032010986
66-67	23.001007513309236	26.507279111901067	26.51810998100961	23.973603393780092
68-69	23.118947633849125	26.307840455950977	26.637523174975712	23.935688735224183
70-71	22.964063307359083	26.199450796272654	26.70567283518897	24.13081306117929
72-73	23.04824489180707	26.26486734730782	26.54472210812201	24.142165652763097
74-75	22.970221332683554	26.402084138989473	26.42436974959702	24.20332477872995
76-77	23.012487828148952	26.206316747758347	26.53454416379864	24.246651260294062
78-79	22.923275573158193	26.253254846441592	26.52936850824642	24.294101072153794
80-81	22.948828482307896	26.352751582146094	26.397052076929263	24.30136785861675
82-83	22.898927153864584	26.33034981548146	26.487250178699938	24.28347285195402
84-85	22.892683185069668	26.26148044903826	26.470206701628147	24.375629664263922
86-87	22.820631833110085	26.413218870168837	26.294998307958096	24.471150988762982
88-89	22.684746762278714	26.3326968737874	26.319289908808337	24.66326645512555
90-91	22.585612274672545	26.278368564388536	26.41634110361821	24.71967805732071
92-93	22.62389961155093	26.118789306444302	26.606427184038285	24.650883897966487
94-95	22.543451618266115	25.97732179134941	26.624523194228495	24.85470339615598
96-97	22.534000893707766	25.890596755986266	26.543504417227386	25.03189793307858
98-99	22.368588496653132	25.980094509870938	26.428000357128862	25.22331663634707
100-101	22.196627022161643	26.011169194080452	26.49543558624735	25.296768197510556
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	88.0
1	67.0
2	69.5
3	93.0
4	85.0
5	103.5
6	170.5
7	391.0
8	589.5
9	619.5
10	555.0
11	475.5
12	472.0
13	498.0
14	567.0
15	660.5
16	751.5
17	843.5
18	967.5
19	1149.0
20	1310.5
21	1462.0
22	1802.0
23	2279.5
24	2800.0
25	3681.5
26	5158.5
27	7178.5
28	9935.5
29	13680.0
30	18309.0
31	23656.0
32	30605.5
33	35367.0
34	38214.5
35	43317.5
36	49772.5
37	56100.5
38	61780.5
39	66657.5
40	70324.0
41	74167.5
42	78530.5
43	83349.5
44	88471.5
45	94213.0
46	98052.5
47	100312.5
48	103506.5
49	105450.0
50	104724.0
51	102288.5
52	100550.5
53	99254.0
54	96437.0
55	91523.0
56	84158.5
57	75063.5
58	67303.5
59	59202.5
60	48802.5
61	39853.5
62	32791.0
63	25208.5
64	17897.0
65	12784.5
66	9571.0
67	7356.5
68	5703.0
69	4320.0
70	3281.5
71	2497.5
72	1759.5
73	1286.0
74	995.5
75	742.5
76	528.5
77	323.5
78	201.0
79	113.0
80	65.5
81	38.0
82	22.0
83	14.0
84	10.5
85	9.5
86	6.0
87	3.5
88	4.5
89	4.0
90	2.0
91	0.5
92	1.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15533320082542731
2	3.635887665614683E-4
3	0.006706192805467082
4	0.004161071439536804
5	0.028279126288114204
6	0.0013331588107253838
7	1.615950073606526E-4
8	0.0016159500736065257
9	0.0
10-11	0.10550134043058605
12-13	2.4239251104097887E-4
14-15	0.005413432746581862
16-17	1.2119625552048943E-4
18-19	1.8179438328073415E-4
20-21	6.867787812827735E-4
22-23	3.029906388012236E-4
24-25	0.0015755513217663626
26-27	0.0023835263585696258
28-29	0.006059812776024472
30-31	0.014583949414298896
32-33	0.014806142549419792
34-35	0.012261021183489516
36-37	6.463800294426104E-4
38-39	1.4139563144057102E-4
40-41	0.0047670527171392515
42-43	0.008180747247633038
44-45	0.007998952864352303
46-47	0.009170516667717035
48-49	0.011917631792848129
50-51	0.005494230250262188
52-53	0.0016361494495266075
54-55	0.0010301681719241604
56-57	0.006100211527864635
58-59	0.009433108554678096
60-61	0.007897955984751895
62-63	0.01834103333543407
64-65	0.034783325334380465
66-67	0.036964857933749276
68-69	0.027147961236589635
70-71	0.04098453374184551
72-73	0.026158191816505638
74-75	0.025572409914823273
76-77	0.24237231166505882
78-79	0.06887987188747817
80-81	0.052962763662453886
82-83	0.02040136967928239
84-85	0.03195541270556905
86-87	0.08002992739536319
88-89	0.26089513938377357
90-91	0.3152718593606332
92-93	0.05068023418348466
94-95	0.005191239611460964
96-97	0.009776497945319481
98-99	0.0010907662996844049
100-101	0.0013533581866454654
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2475324.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.59395497335994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.52706882805998	51.21034789188303
2	11.231643912664508	14.285293145175979
3	3.3546450806120234	6.400054446241333
4	1.5576029482640292	3.9621652703310155
5	0.8670207187012353	2.7568638273028268
6	0.5533448649343078	2.1113633055223384
7	0.35997854206306934	1.602472143673421
8	0.2714095349293602	1.3808004594910634
9	0.20827382850485562	1.192046082486037
>10	1.0154778688975898	11.128424209725626
>50	0.03922215527641951	1.676550346757091
>100	0.012846441377047766	1.4318816720232392
>500	0.0011467375165891544	0.5375830229750087
>1k	3.1853819905127356E-4	0.3241541764119652
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.039875184016315E-5	0.0
3	0.0	0.0	0.0	4.039875184016315E-5	0.0
4	0.0	0.0	0.0	8.07975036803263E-5	0.0
5	0.0	0.0	0.0	1.2119625552048943E-4	0.0
6	0.0	0.0	0.0	1.2119625552048943E-4	8.07975036803263E-5
7	0.0	0.0	0.0	1.2119625552048943E-4	1.2119625552048943E-4
8	0.0	0.0	0.0	1.2119625552048943E-4	1.2119625552048943E-4
9	0.0	0.0	0.0	1.615950073606526E-4	1.2119625552048943E-4
10-11	0.0	0.0	0.0	1.615950073606526E-4	1.2119625552048943E-4
12-13	0.0	0.0	0.0	2.0199375920081574E-4	2.0199375920081574E-4
14-15	0.0	0.0	0.0	2.0199375920081574E-4	2.4239251104097887E-4
16-17	0.0	0.0	0.0	2.0199375920081574E-4	2.4239251104097887E-4
18-19	0.0	0.0	0.0	2.4239251104097887E-4	2.8279126288114205E-4
20-21	0.0	0.0	0.0	2.4239251104097887E-4	2.8279126288114205E-4
22-23	0.0	0.0	0.0	4.2418689432171305E-4	2.8279126288114205E-4
24-25	0.0	0.0	0.0	6.867787812827735E-4	2.8279126288114205E-4
26-27	0.0	0.0	0.0	0.0011109656756044866	3.029906388012236E-4
28-29	0.0	0.0	0.0	0.0036156882896946014	3.231900147213052E-4
30-31	0.0	0.0	0.0	0.014947538180860365	3.231900147213052E-4
32-33	0.0	0.0	0.0	0.04324686384489465	3.231900147213052E-4
34-35	0.0	0.0	0.0	0.08134288683016849	3.231900147213052E-4
36-37	0.0	0.0	0.0	0.13200292163773308	3.231900147213052E-4
38-39	0.0	0.0	0.0	0.20138777792321327	3.635887665614683E-4
40-41	0.0	0.0	0.0	0.27790301390848227	3.635887665614683E-4
42-43	0.0	0.0	0.0	0.366234884806999	3.635887665614683E-4
44-45	0.0	0.0	0.0	0.4888854953937343	3.635887665614683E-4
46-47	0.0	0.0	0.0	0.6299781361955041	4.039875184016315E-4
48-49	0.0	0.0	0.0	0.7617790640740363	4.039875184016315E-4
50-51	0.0	0.0	0.0	0.896912888979382	4.039875184016315E-4
52-53	0.0	0.0	0.0	1.042994775633412	4.8478502208195774E-4
54-55	0.0	0.0	0.0	1.2298996010219267	5.251837739221209E-4
56-57	0.0	0.0	0.0	1.4606774709088588	5.251837739221209E-4
58-59	0.0	0.0	0.0	1.6862439018084099	5.251837739221209E-4
60-61	0.0	0.0	0.0	1.9005794796963953	5.251837739221209E-4
62-63	0.0	0.0	0.0	2.134023667204778	5.655825257622841E-4
64-65	0.0	0.0	0.0	2.4124114661353424	5.655825257622841E-4
66-67	0.0	0.0	0.0	2.7585883706537	5.655825257622841E-4
68-69	0.0	0.0	0.0	3.117389885122109	5.655825257622841E-4
70-71	0.0	0.0	0.0	3.4670208829228013	5.655825257622841E-4
72-73	0.0	0.0	0.0	3.827256553081536	6.665794053626919E-4
74-75	0.0	0.0	0.0	4.243202102027856	6.867787812827735E-4
76-77	0.0	0.0	0.0	4.742712469155553	7.06978157202855E-4
78-79	0.0	0.0	0.0	5.287388640840552	7.271775331229366E-4
80-81	0.0	0.0	0.0	5.810431280915145	7.271775331229366E-4
82-83	0.0	0.0	0.0	6.335675652965026	8.483737886434261E-4
84-85	0.0	0.0	0.0	6.903944695724681	8.483737886434261E-4
86-87	0.0	0.0	0.0	7.5734530105957845	8.685731645635077E-4
88-89	0.0	0.0	0.0	8.308912287845954	9.695700441639155E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2510	0.0	56.798714	1
GTATCAA	4490	0.0	41.70046	1
TCAACGC	5785	0.0	32.078285	4
CAACGCA	5875	0.0	31.40226	5
ATCAACG	5980	0.0	30.952887	3
AACGCAG	6190	0.0	29.879246	6
TATCAAC	6220	0.0	29.529652	2
ACGCAGA	7080	0.0	25.51833	7
CGCAGAG	7555	0.0	23.78807	8
GTACATG	5960	0.0	21.289034	1
GCAGAGT	8410	0.0	20.35206	9
GTGGTAT	1195	0.0	19.48584	1
TACATGG	6380	0.0	19.34151	2
AGAGTAC	7255	0.0	18.775396	10-11
TGGTATC	1215	0.0	18.750061	2
ACATGGG	6335	0.0	18.579876	3
CAGAGTA	7930	0.0	18.34637	10-11
GAGTACT	4705	0.0	18.001234	12-13
GTATAGA	740	0.0	17.98114	1
GTCCTAA	725	0.0	17.697695	1
>>END_MODULE