FastQCFastQC Report
Mon 15 Aug 2016
SRR2921404_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921404_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3829041
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32850.050.271081
GTATCAA53050.036.138521
TCAACGC59550.031.4800244
CAACGCA60550.030.8801295
ATCAACG60850.030.6498973
TATCAAC64450.029.011892
AACGCAG64950.028.7783956
GTACATG89800.025.9993881
TACATGG93950.023.89282
ACGCAGA76750.023.5539727
ACATGGG93950.022.5791593
CGCAGAG79950.021.663378
CATGGGG83700.019.4486034
GTATTAG10900.018.285091
GAGTACA70450.017.3785291
GCAGAGT96050.017.1431039
CAGAGTA85650.016.87041510-11
ATGGGGG46600.016.3959645
GAGTACT39500.015.97823712-13
AGAGTAC75750.015.97435610-11
Produced by FastQC (version 0.11.5)