##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921404_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3829041
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.367618419337898	31.0	31.0	34.0	26.0	34.0
2	30.428036941887015	31.0	31.0	34.0	26.0	34.0
3	30.476549350085307	33.0	31.0	34.0	26.0	34.0
4	33.93187745965635	37.0	35.0	37.0	30.0	37.0
5	33.925952477395775	37.0	35.0	37.0	30.0	37.0
6	33.971240057236265	37.0	35.0	37.0	30.0	37.0
7	33.8897201675302	37.0	35.0	37.0	30.0	37.0
8	33.853114918330725	37.0	35.0	37.0	29.0	37.0
9	35.41827209476211	39.0	37.0	39.0	30.0	39.0
10-11	35.31280704489714	39.0	36.0	39.0	29.0	39.0
12-13	35.20756502737892	39.0	35.0	39.0	29.0	39.0
14-15	36.34525106939309	40.0	37.0	41.0	28.0	41.0
16-17	36.29637551543585	40.0	37.0	41.0	28.0	41.0
18-19	36.252863314861344	40.0	37.0	41.0	27.0	41.0
20-21	36.19571336530478	40.0	37.0	41.0	27.0	41.0
22-23	36.081172152505026	40.0	36.5	41.0	27.0	41.0
24-25	35.99032708189857	40.0	36.0	41.0	27.0	41.0
26-27	35.85022868650401	40.0	36.0	41.0	27.0	41.0
28-29	35.71545734297439	40.0	36.0	41.0	26.5	41.0
30-31	35.57781948534894	40.0	36.0	41.0	25.5	41.0
32-33	35.408863342022194	40.0	35.5	41.0	25.0	41.0
34-35	35.2508506699197	39.0	35.0	41.0	25.0	41.0
36-37	35.08929154845822	39.0	35.0	41.0	24.0	41.0
38-39	35.314229333141114	39.5	35.0	41.0	25.0	41.0
40-41	35.43776183122615	40.0	35.0	41.0	25.0	41.0
42-43	35.28549276437625	40.0	35.0	41.0	24.0	41.0
44-45	35.175559624459495	40.0	35.0	41.0	24.0	41.0
46-47	35.04135408839968	40.0	35.0	41.0	23.0	41.0
48-49	34.90878407935564	40.0	35.0	41.0	22.5	41.0
50-51	33.222057167839154	37.0	32.5	39.5	18.5	40.0
52-53	33.381368729141315	37.5	33.5	39.5	19.0	40.0
54-55	34.25027611352294	39.0	34.0	40.5	19.5	41.0
56-57	34.28223411553964	39.0	34.0	41.0	20.0	41.0
58-59	34.11864759348359	39.0	34.0	41.0	18.5	41.0
60-61	33.89451340427016	38.0	34.0	41.0	18.0	41.0
62-63	33.621620139350824	38.0	34.0	40.0	15.0	41.0
64-65	33.34428346418855	37.0	34.0	40.0	13.0	41.0
66-67	33.01628645397111	37.0	33.0	40.0	11.0	41.0
68-69	32.6596054991315	36.0	33.0	39.0	8.0	41.0
70-71	32.28122642196832	35.5	33.0	39.0	7.0	41.0
72-73	31.921195542173614	35.0	32.0	38.5	2.0	40.0
74-75	31.524208933777416	35.0	32.0	37.0	2.0	39.5
76-77	31.139342853732828	35.0	32.0	37.0	2.0	39.0
78-79	30.7862106987102	35.0	32.0	36.0	2.0	39.0
80-81	30.429323556472756	35.0	31.0	36.0	2.0	37.5
82-83	30.12521477309854	35.0	31.0	35.5	2.0	37.0
84-85	29.88384258617236	35.0	31.0	35.0	2.0	37.0
86-87	29.654487376865383	35.0	31.0	35.0	2.0	36.0
88-89	29.45630315789254	35.0	31.0	35.0	2.0	36.0
90-91	29.208815732189862	35.0	31.0	35.0	2.0	36.0
92-93	29.027859586773815	34.5	30.5	35.0	2.0	35.0
94-95	28.831441214654006	34.0	30.0	35.0	2.0	35.0
96-97	28.595744730860808	34.0	30.0	35.0	2.0	35.0
98-99	28.378219507182084	34.0	30.0	35.0	2.0	35.0
100-101	27.347826257279564	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	135280.0
3	22594.0
4	15310.0
5	11579.0
6	10310.0
7	10556.0
8	11099.0
9	12017.0
10	13124.0
11	13980.0
12	14064.0
13	14307.0
14	15301.0
15	16526.0
16	16425.0
17	17226.0
18	16218.0
19	15316.0
20	15150.0
21	15991.0
22	17554.0
23	20030.0
24	23429.0
25	27774.0
26	33212.0
27	39157.0
28	46349.0
29	55237.0
30	65801.0
31	80441.0
32	100336.0
33	129767.0
34	178902.0
35	261481.0
36	434237.0
37	799752.0
38	924569.0
39	178626.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.575341187881776	18.165600861458735	13.298259705978344	24.960798244681143
2	15.872058929596191	19.478956627541244	39.53057856077402	25.118405882088542
3	19.102922147827776	23.32176131577132	29.566966497415244	28.008350038985665
4	13.409293073730181	15.980133606357747	36.68206348758377	33.92850983232831
5	14.362960653886365	36.90956071811276	34.59824665600756	14.12923197199332
6	33.459070540741386	35.222334396888485	17.163324717796545	14.155270344573584
7	28.876851001497204	31.743637402741776	20.954517577265893	18.424994018495127
8	26.383939894765685	34.755326171256144	19.989478135916467	18.87125579806171
9	26.463576906258666	15.767040779383207	19.58624523216565	38.183137082192474
10-11	25.45484941969965	25.265895893359524	28.064267969980587	21.214986716960237
12-13	25.967942392179634	22.858852088681875	27.629561756578745	23.54364376255975
14-15	23.20456409727826	24.473914787788576	24.98486877541004	27.336652339523127
16-17	23.389589103507586	27.487176955145337	25.916827460882985	23.20640648046409
18-19	23.409928572613403	26.87912070401566	27.118661777799662	22.59228894557128
20-21	23.962652769844684	26.102713895415686	27.04611246305696	22.88852087168267
22-23	23.750360906833127	26.027921450995713	26.587160469956224	23.634557172214944
24-25	23.525083842729956	26.206261476447224	26.66896413339172	23.599690547431106
26-27	23.45767930450793	26.381051166714148	26.679386406441015	23.48188312233691
28-29	23.44529859150846	26.606140481368374	26.56635797128006	23.382202955843113
30-31	23.795992780632034	26.2014083513774	26.548298459746277	23.45430040824429
32-33	23.493978814422007	26.241746686431476	26.461678336673682	23.802596162472824
34-35	23.879333581284946	26.31839651224017	26.466031325482458	23.33623858099242
36-37	23.85412784543572	26.33060789248738	26.407247041838527	23.408017220238378
38-39	23.672168449062035	26.364821048532438	26.523369853579048	23.439640648826476
40-41	23.86809839224038	26.163784136930207	26.554914799931417	23.413202670897995
42-43	23.71815777172061	26.145647554829903	26.43350553860956	23.702689134839925
44-45	23.691975495927743	26.26753807026571	26.44702858097568	23.593457852830866
46-47	23.825175678853025	26.26929909413864	26.179554274811746	23.72597095219659
48-49	23.538435048564878	26.366078535941256	26.387719332217884	23.707767083275982
50-51	23.698404989399567	26.337182739323552	26.406984405771283	23.557427865505602
52-53	23.860339561599464	26.221221533594573	26.42039101960463	23.498047885201338
54-55	23.526965036648583	26.050365962480015	26.6283040641854	23.794364936686005
56-57	23.561720018686184	26.292951723434015	26.40327610144452	23.742052156435285
58-59	23.569481326166553	26.197499757271963	26.573329121685653	23.659689794875835
60-61	23.65003582212597	26.124988141077022	26.6655980005038	23.55937803629321
62-63	23.57628745445469	26.125804178555285	26.724970770526223	23.572937596463806
64-65	23.4215538420772	26.016023239913505	26.678413116942174	23.884009801067123
66-67	23.417975419906657	26.208900699615455	26.509092907414463	23.864030973063432
68-69	23.557550899501027	26.143740651459186	26.5466634148486	23.75204503419119
70-71	23.416520050878262	25.91659559905386	26.643284015745937	24.023600334321934
72-73	23.438443811085037	25.952028803389005	26.622856279383456	23.986671106142506
74-75	23.3971805291826	26.115311159901626	26.56345300790391	23.92405530301187
76-77	23.421480709508494	25.877995958509175	26.658467336689988	24.04205599529234
78-79	23.3645406486798	25.9077248574223	26.68951730993151	24.03821718396639
80-81	23.39599603464127	26.069197472901745	26.583411582642725	23.951394909814265
82-83	23.43772533908666	26.096710203105193	26.612109360992985	23.85345509681516
84-85	23.453193670030004	26.024534601058207	26.64313412241599	23.8791376064958
86-87	23.33822500027486	26.07580897054581	26.616776201263736	23.969189827915592
88-89	23.356912173238705	25.927849927849927	26.637479426595075	24.077758472316297
90-91	23.294645152272054	25.920435620943604	26.729790728343684	24.05512849844066
92-93	23.32762184212803	25.81711387193424	26.892013811765285	23.96325047417244
94-95	23.268390207492757	25.730942327430697	26.912377766121754	24.088289698954792
96-97	23.305223912150215	25.672609565807814	26.8991334692007	24.12303305284127
98-99	23.20774994787374	25.578699658892035	26.85785780636727	24.355692586866954
100-101	23.34616200728932	25.440790680206828	27.025902698864847	24.18714461363901
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	31.0
1	23.5
2	33.5
3	67.5
4	85.5
5	100.5
6	158.5
7	302.5
8	480.5
9	585.0
10	567.5
11	510.0
12	528.5
13	614.5
14	715.5
15	826.5
16	938.0
17	1020.0
18	1144.0
19	1326.0
20	1455.0
21	1560.5
22	1733.0
23	1996.0
24	2365.5
25	2909.0
26	3859.0
27	5492.5
28	8008.5
29	11982.0
30	17640.0
31	25260.0
32	35244.0
33	44125.0
34	52212.5
35	61669.5
36	73530.5
37	85908.5
38	96931.5
39	107413.0
40	116193.0
41	124497.0
42	132969.0
43	141576.0
44	150513.0
45	160172.5
46	165835.0
47	168166.0
48	171337.5
49	172827.5
50	169820.5
51	164009.5
52	159122.5
53	153906.0
54	147766.0
55	138724.5
56	125629.5
57	111724.0
58	99740.5
59	87311.0
60	71722.5
61	57932.5
62	47233.5
63	36011.5
64	25858.0
65	18961.5
66	14269.0
67	10986.5
68	8372.0
69	6262.5
70	4818.5
71	3584.0
72	2604.0
73	1914.0
74	1422.5
75	1043.0
76	730.5
77	517.5
78	367.0
79	250.0
80	180.5
81	138.0
82	98.0
83	91.0
84	84.5
85	58.0
86	45.5
87	47.5
88	43.5
89	34.0
90	28.0
91	26.5
92	27.0
93	24.5
94	20.5
95	14.0
96	9.0
97	8.0
98	7.0
99	7.5
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.053381512498821504
2	0.061973742250344147
3	0.052049586306336236
4	0.05946658706448951
5	0.051031054512082785
6	0.017915713098919546
7	0.014964582515569825
8	0.021467516278880272
9	0.02044898448462683
10-11	0.02222488607460719
12-13	0.028427483539612136
14-15	0.044149435850908886
16-17	0.03047760522804535
18-19	0.023178127369229005
20-21	0.026625465749779125
22-23	0.048993990923575904
24-25	0.04275221915879198
26-27	0.02957659633312884
28-29	0.018947302993099317
30-31	0.011778406133546233
32-33	0.009179844248207319
34-35	0.008801159350343857
36-37	0.0015147395914538392
38-39	0.006568224262942079
40-41	0.0036954422791503146
42-43	0.21955889216124874
44-45	0.19301177501102756
46-47	0.1998019869727172
48-49	0.1973078898867889
50-51	0.19584538269504034
52-53	0.20037654336947552
54-55	0.19877039707853744
56-57	0.2098697820159147
58-59	0.20610904923713275
60-61	0.2109797205096524
62-63	0.20849868152365042
64-65	0.20967391051701978
66-67	0.19994562607190675
68-69	0.19819584068177906
70-71	0.1821996682720295
72-73	0.18982559862900397
74-75	0.18960361093025643
76-77	0.18761877974145486
78-79	0.21263809920029583
80-81	0.22077329545439708
82-83	0.21963724076080668
84-85	0.21501467338688723
86-87	0.2334527104828598
88-89	0.23187268039177433
90-91	0.23084109049759458
92-93	0.23411867357910243
94-95	0.24459126972001607
96-97	0.24508747751721643
98-99	0.23477157857541875
100-101	0.24399059712340507
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3829041.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.79667837334631
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.64411840939009	44.47622900952234
2	13.54152282398613	15.923931243344816
3	4.69732858473801	8.285619540323003
4	2.0781002130330797	4.8874155941315385
5	1.133078498824205	3.331062603356042
6	0.7007913099318555	2.472252075413962
7	0.44498866380569835	1.8314698741898146
8	0.32090819954474303	1.5094668956801516
9	0.2314375881397326	1.2246985290019363
>10	1.1386739643754897	11.51735173315377
>50	0.049900300914521564	1.9924985522416405
>100	0.017638401552791384	1.785418324703611
>500	0.0010235282756929425	0.41432664296261607
>1k	4.895134878639372E-4	0.3482593819747444
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.61161998526524E-5
2	0.0	2.61161998526524E-5	0.0	0.0	2.61161998526524E-5
3	0.0	2.61161998526524E-5	0.0	2.61161998526524E-5	2.61161998526524E-5
4	0.0	2.61161998526524E-5	0.0	5.22323997053048E-5	2.61161998526524E-5
5	0.0	2.61161998526524E-5	0.0	5.22323997053048E-5	2.61161998526524E-5
6	0.0	2.61161998526524E-5	0.0	5.22323997053048E-5	2.61161998526524E-5
7	0.0	2.61161998526524E-5	0.0	5.22323997053048E-5	5.22323997053048E-5
8	0.0	2.61161998526524E-5	0.0	5.22323997053048E-5	5.22323997053048E-5
9	0.0	2.61161998526524E-5	0.0	5.22323997053048E-5	5.22323997053048E-5
10-11	0.0	2.61161998526524E-5	0.0	5.22323997053048E-5	5.22323997053048E-5
12-13	0.0	2.61161998526524E-5	0.0	1.044647994106096E-4	1.044647994106096E-4
14-15	0.0	2.61161998526524E-5	0.0	1.436390991895882E-4	1.566971991159144E-4
16-17	0.0	2.61161998526524E-5	0.0	1.566971991159144E-4	1.828133989685668E-4
18-19	0.0	2.61161998526524E-5	0.0	1.828133989685668E-4	1.828133989685668E-4
20-21	0.0	2.61161998526524E-5	0.0	2.089295988212192E-4	2.350457986738716E-4
22-23	0.0	5.22323997053048E-5	0.0	2.742200984528502E-4	2.481038986001978E-4
24-25	0.0	5.22323997053048E-5	0.0	3.656267979371336E-4	2.61161998526524E-4
26-27	0.0	5.22323997053048E-5	0.0	5.87614496684679E-4	3.26452498158155E-4
28-29	0.0	5.22323997053048E-5	0.0	0.0015408557913064915	3.395105980844812E-4
30-31	0.0	5.22323997053048E-5	0.0	0.006711863362131667	3.395105980844812E-4
32-33	0.0	5.22323997053048E-5	0.0	0.021454458178953945	3.656267979371336E-4
34-35	0.0	5.22323997053048E-5	0.0	0.042582463859749745	3.91742997789786E-4
36-37	0.0	5.22323997053048E-5	0.0	0.07364768358447976	3.91742997789786E-4
38-39	0.0	5.22323997053048E-5	0.0	0.12136198071527571	3.91742997789786E-4
40-41	0.0	5.22323997053048E-5	0.0	0.17589260600761392	3.91742997789786E-4
42-43	0.0	5.22323997053048E-5	0.0	0.23720038516171543	3.91742997789786E-4
44-45	0.0	5.22323997053048E-5	0.0	0.3187221030017699	3.91742997789786E-4
46-47	0.0	5.22323997053048E-5	0.0	0.41532592625673115	3.91742997789786E-4
48-49	0.0	5.22323997053048E-5	0.0	0.510832869117881	4.439753974950908E-4
50-51	1.30580999263262E-5	5.22323997053048E-5	0.0	0.6093170587622332	4.700915973477432E-4
52-53	2.61161998526524E-5	5.22323997053048E-5	0.0	0.715831979861276	4.831496972740694E-4
54-55	2.61161998526524E-5	9.14066994842834E-5	0.0	0.8491421220091402	4.962077972003956E-4
56-57	2.61161998526524E-5	1.044647994106096E-4	0.0	1.0064138775218128	4.962077972003956E-4
58-59	2.61161998526524E-5	1.30580999263262E-4	0.0	1.173191929780851	4.962077972003956E-4
60-61	2.61161998526524E-5	1.30580999263262E-4	0.0	1.3431039260222075	4.962077972003956E-4
62-63	2.61161998526524E-5	1.30580999263262E-4	0.0	1.5205635040209806	5.22323997053048E-4
64-65	2.61161998526524E-5	1.30580999263262E-4	0.0	1.7296759162411686	5.22323997053048E-4
66-67	2.61161998526524E-5	1.30580999263262E-4	0.0	1.9823762660154332	5.22323997053048E-4
68-69	2.61161998526524E-5	1.30580999263262E-4	0.0	2.252822573589575	5.22323997053048E-4
70-71	2.61161998526524E-5	1.30580999263262E-4	0.0	2.5296542920276903	5.614982968320265E-4
72-73	2.61161998526524E-5	1.30580999263262E-4	0.0	2.817559279203331	6.920792960952887E-4
74-75	2.61161998526524E-5	1.30580999263262E-4	0.0	3.1335652974204242	7.051373960216148E-4
76-77	5.22323997053048E-5	1.30580999263262E-4	0.0	3.5086853340039976	7.051373960216148E-4
78-79	5.22323997053048E-5	1.30580999263262E-4	0.0	3.9204333408809147	7.051373960216148E-4
80-81	5.22323997053048E-5	1.30580999263262E-4	0.0	4.3439597538913794	7.18195495947941E-4
82-83	5.22323997053048E-5	1.30580999263262E-4	0.0	4.768191304297865	7.83485995579572E-4
84-85	5.22323997053048E-5	1.30580999263262E-4	0.0	5.227810305504694	7.83485995579572E-4
86-87	5.22323997053048E-5	1.30580999263262E-4	0.0	5.745825129582054	7.965440955058983E-4
88-89	5.22323997053048E-5	1.30580999263262E-4	0.0	6.312298562486012	8.096021954322244E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3285	0.0	50.27108	1
GTATCAA	5305	0.0	36.13852	1
TCAACGC	5955	0.0	31.480024	4
CAACGCA	6055	0.0	30.880129	5
ATCAACG	6085	0.0	30.649897	3
TATCAAC	6445	0.0	29.01189	2
AACGCAG	6495	0.0	28.778395	6
GTACATG	8980	0.0	25.999388	1
TACATGG	9395	0.0	23.8928	2
ACGCAGA	7675	0.0	23.553972	7
ACATGGG	9395	0.0	22.579159	3
CGCAGAG	7995	0.0	21.66337	8
CATGGGG	8370	0.0	19.448603	4
GTATTAG	1090	0.0	18.28509	1
GAGTACA	7045	0.0	17.378529	1
GCAGAGT	9605	0.0	17.143103	9
CAGAGTA	8565	0.0	16.870415	10-11
ATGGGGG	4660	0.0	16.395964	5
GAGTACT	3950	0.0	15.978237	12-13
AGAGTAC	7575	0.0	15.974356	10-11
>>END_MODULE