FastQCFastQC Report
Mon 15 Aug 2016
SRR2921404_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921404_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3829041
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33950.051.2357441
GTATCAA56900.037.9206121
TCAACGC67950.030.84
CAACGCA70750.029.5703775
ATCAACG70800.029.493143
TATCAAC73950.028.6222612
AACGCAG75100.027.8546646
GTACATG99200.024.2900281
TACATGG101050.023.1065982
ACGCAGA89850.022.8586627
ACATGGG103700.021.8293693
CGCAGAG92400.021.5589318
GTATATA12800.021.1639441
CATGGGG87150.018.895734
AGAGTAC90550.017.1952310-11
GCAGAGT109200.017.0231259
TATACTG15450.016.3109045
CAGAGTA100250.016.07683210-11
GTATAGC14250.016.0087761
GAGTACT46150.015.47222912-13
Produced by FastQC (version 0.11.5)