FastQCFastQC Report
Mon 15 Aug 2016
SRR2921403_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921403_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3953771
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31300.048.8215451
GTATCAA56550.042.04411
TCAACGC66250.034.600134
CAACGCA67400.033.9389275
ATCAACG70300.032.673493
TATCAAC72200.032.1435362
AACGCAG72450.031.6934536
GTACATG117350.028.6723371
TACATGG122250.026.748062
ACGCAGA88050.025.3274547
ACATGGG126650.025.143333
CGCAGAG90250.023.9752228
CATGGGG114050.023.552534
GCAGAGT108050.019.1031079
ATGGGGG72100.019.0885835
GAGTACA86400.018.3456341
GTATATA10750.017.6584051
AGAGTAC88450.017.27440310-11
GTATAGA11050.017.1789931
TAGACAG20550.016.858625
Produced by FastQC (version 0.11.5)