##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921403_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3953771
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.187819931908045	31.0	31.0	34.0	26.0	34.0
2	30.241496535838824	31.0	31.0	34.0	26.0	34.0
3	30.291989343844143	33.0	31.0	34.0	26.0	34.0
4	33.739343022142656	37.0	35.0	37.0	28.0	37.0
5	33.723231315116635	37.0	35.0	37.0	28.0	37.0
6	33.766492545976995	37.0	35.0	37.0	29.0	37.0
7	33.679634455308616	37.0	35.0	37.0	28.0	37.0
8	33.64223598180067	37.0	35.0	37.0	28.0	37.0
9	35.18100365448581	39.0	35.0	39.0	28.0	39.0
10-11	35.070069940823586	39.0	35.0	39.0	28.0	39.0
12-13	34.95223699096381	39.0	35.0	39.0	27.0	39.0
14-15	36.06729335108179	40.0	36.0	41.0	27.0	41.0
16-17	36.00870788925307	40.0	36.0	41.0	27.0	41.0
18-19	35.952382674666794	40.0	36.0	41.0	27.0	41.0
20-21	35.88559795192994	40.0	36.0	41.0	27.0	41.0
22-23	35.76818649840874	40.0	36.0	41.0	27.0	41.0
24-25	35.66544382565404	40.0	36.0	41.0	26.0	41.0
26-27	35.51663386675657	40.0	36.0	41.0	25.0	41.0
28-29	35.37636752356168	40.0	36.0	41.0	25.0	41.0
30-31	35.23348228817501	39.5	35.0	41.0	24.5	41.0
32-33	35.05661101768413	39.0	35.0	41.0	24.0	41.0
34-35	34.89565227728161	39.0	35.0	41.0	23.5	41.0
36-37	34.73203809730002	39.0	35.0	41.0	23.0	41.0
38-39	34.97366425622526	39.0	35.0	41.0	24.0	41.0
40-41	35.095931706717465	40.0	35.0	41.0	23.5	41.0
42-43	34.941653676958026	40.0	35.0	41.0	22.5	41.0
44-45	34.824789422553806	40.0	35.0	41.0	22.0	41.0
46-47	34.67997703964139	39.5	35.0	41.0	20.5	41.0
48-49	34.5395890404376	39.0	35.0	41.0	19.0	41.0
50-51	32.83282618037312	37.0	32.5	39.5	15.5	40.0
52-53	32.98257827780112	37.5	32.5	39.5	14.0	40.0
54-55	33.83222586740608	38.5	34.0	40.0	14.0	41.0
56-57	33.83766004657326	38.5	34.0	41.0	13.0	41.0
58-59	33.65238679731325	38.0	34.0	40.5	12.5	41.0
60-61	33.4005946474897	37.5	33.5	40.0	9.0	41.0
62-63	33.1062904250145	37.0	33.0	40.0	7.5	41.0
64-65	32.81188515976267	36.5	33.0	40.0	7.0	41.0
66-67	32.470125862119986	36.0	33.0	39.0	2.0	41.0
68-69	32.1031004324732	35.5	32.5	39.0	2.0	41.0
70-71	31.7291490832423	35.0	32.0	38.5	2.0	40.5
72-73	31.377709912890758	35.0	32.0	37.0	2.0	39.5
74-75	30.989341188450215	35.0	32.0	37.0	2.0	39.0
76-77	30.628788566662053	35.0	31.0	36.0	2.0	39.0
78-79	30.30195843411265	35.0	31.0	36.0	2.0	38.0
80-81	29.97427999750112	35.0	31.0	35.5	2.0	37.0
82-83	29.696548307931845	35.0	31.0	35.0	2.0	37.0
84-85	29.48080187244026	35.0	31.0	35.0	2.0	36.0
86-87	29.272857608596958	35.0	30.0	35.0	2.0	36.0
88-89	29.090560126016406	35.0	30.0	35.0	2.0	36.0
90-91	28.858335245010394	34.0	30.0	35.0	2.0	35.0
92-93	28.683886471927686	34.0	30.0	35.0	2.0	35.0
94-95	28.49574621291926	34.0	30.0	35.0	2.0	35.0
96-97	28.25614950891187	34.0	29.0	35.0	2.0	35.0
98-99	28.041539203965026	34.0	29.0	35.0	2.0	35.0
100-101	27.015157301725367	33.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	156906.0
3	25618.0
4	17576.0
5	13265.0
6	11847.0
7	12019.0
8	12645.0
9	13428.0
10	14566.0
11	15297.0
12	15317.0
13	15199.0
14	16073.0
15	16482.0
16	16676.0
17	17585.0
18	16638.0
19	15862.0
20	16245.0
21	16782.0
22	18547.0
23	21289.0
24	24668.0
25	29725.0
26	35405.0
27	42013.0
28	49843.0
29	59503.0
30	71530.0
31	86155.0
32	108101.0
33	140605.0
34	196101.0
35	286296.0
36	479042.0
37	854275.0
38	855531.0
39	139100.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.36689521491085	17.632115351280632	13.092841028859329	24.908148404949195
2	16.158203271307126	19.086243827579157	38.99770413276348	25.757848768350232
3	19.42629658702191	23.097435648279593	28.95358011720235	28.522687647496152
4	13.75320474779701	15.45491285844397	35.750522768355474	35.041359625403544
5	14.863129787952264	36.786023544439736	33.79134713245405	14.55949953515395
6	34.31497198365819	34.26652837682296	16.89267780574493	14.525821833773922
7	29.74735225282381	31.272785499846705	20.387602664392038	18.592259582937448
8	27.363851508179344	34.273712384057966	19.422051154265997	18.940384953496697
9	27.13385696100316	15.508143820188335	18.894538135704703	38.4634610831038
10-11	26.14847228959553	24.862021924451525	27.256268669082196	21.733237116870747
12-13	26.63253366391115	22.147836591675258	27.18976389407174	24.029865850341857
14-15	23.844221442482894	23.757848175862904	24.282729926597273	28.115200455056925
16-17	24.249071589885165	26.865419020766257	25.05722512868459	23.828284260663988
18-19	24.332004377093348	26.381499532679133	26.16081074636879	23.12568534385873
20-21	24.76673639929193	25.48148526696953	26.180156585963527	23.571621747775012
22-23	24.70697773753247	25.10658546808977	25.944061243832202	24.24237555054556
24-25	24.502105845127712	25.365698446014072	25.800531110714697	24.331664598143515
26-27	24.246500967845666	25.599549460316307	25.85468577209948	24.29926379973855
28-29	24.369816775389534	25.86830514515875	25.65174903118302	24.110129048268703
30-31	24.765774469875694	25.521422702002088	25.539230634095183	24.17357219402703
32-33	24.45773368192709	25.542746916078485	25.523055045166092	24.476464356828338
34-35	24.733945478584484	25.663735617196153	25.565314478429933	24.037004425789423
36-37	24.723026458393456	25.52170527126752	25.661548360694507	24.09371990964452
38-39	24.612556751720525	25.605458130986996	25.585222970599958	24.196762146692517
40-41	24.701749149833002	25.408377354004607	25.653759135580295	24.236114360582096
42-43	24.52156446881948	25.465450804489052	25.519113403621958	24.493871323069506
44-45	24.40924937639998	25.49808808275843	25.66972855564783	24.422933985193758
46-47	24.61489743713536	25.40673949601342	25.513004398728317	24.4653586681229
48-49	24.41919195759022	25.5147138206363	25.571152406483517	24.494941815289966
50-51	24.4604068591077	25.54642295262772	25.69789525222913	24.295274936035455
52-53	24.656268793988865	25.44102593605311	25.605646389032433	24.297058880925597
54-55	24.157950125481445	25.31018721793602	25.87422964023161	24.657633016350918
56-57	24.323715678547718	25.461965141434945	25.703562920925265	24.510756259092076
58-59	24.443430639590474	25.402528049128982	25.737311757031527	24.416729554249013
60-61	24.453852396814284	25.37802614817094	25.901666310974758	24.266455144040012
62-63	24.43430450191651	25.38927722621458	25.863810822230043	24.31260744963886
64-65	24.226413611835586	25.25144535774664	25.872334547408492	24.649806483009286
66-67	24.297692724885827	25.360790515436744	25.76016655768948	24.58135020198795
68-69	24.248149502601606	25.34108784689461	25.843871671138473	24.56689097936531
70-71	24.22965372616352	25.17819713404783	25.838064985637804	24.754084154150846
72-73	24.19370758618907	25.41656588315059	25.726867189647063	24.66285934101328
74-75	24.16272183238814	25.499832813613217	25.78299168984195	24.554453664156696
76-77	24.272499945517954	25.140855099545036	25.805814830976416	24.780830123960595
78-79	24.112005955509332	25.188362373073776	25.9320786529741	24.76755301844279
80-81	24.266331756375322	25.326779616505917	25.77141282611104	24.635475801007722
82-83	24.238612344357755	25.30613238150872	25.889303240608424	24.5659520335251
84-85	24.2209273058126	25.347722570200382	25.838487102333463	24.59286302165356
86-87	24.117482272829832	25.494991773376164	25.79595437709627	24.591571576697742
88-89	23.963974264361962	25.285766328336507	25.958222558662303	24.792036848639228
90-91	24.067317302567243	25.17612357645669	26.015270233185728	24.74128888779034
92-93	24.17925980667737	25.06837151257751	26.06119876256277	24.691169918182354
94-95	24.106597959003086	25.025551127228766	26.06666289727851	24.801188016489636
96-97	24.03228483023474	24.899978308930777	26.167137358220465	24.90059950261402
98-99	23.842882440704436	24.875945477615932	26.151413320285418	25.129758761394218
100-101	24.024522474392157	24.83323320374168	26.241694621183704	24.900549700682458
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	58.0
1	51.0
2	67.0
3	85.5
4	87.5
5	115.0
6	188.5
7	394.5
8	596.0
9	660.5
10	616.5
11	535.0
12	551.0
13	630.0
14	731.0
15	844.5
16	972.0
17	1068.5
18	1171.5
19	1340.0
20	1466.0
21	1557.0
22	1688.5
23	1817.5
24	2034.0
25	2396.5
26	2947.5
27	3903.5
28	5462.0
29	8006.0
30	12027.0
31	17646.5
32	25229.5
33	32353.5
34	38746.5
35	46638.0
36	57405.5
37	68941.0
38	79621.0
39	90078.5
40	99598.5
41	108338.0
42	117564.5
43	128088.5
44	138378.0
45	148664.0
46	157592.0
47	165498.0
48	173028.5
49	177181.0
50	177922.0
51	178658.0
52	178496.0
53	178016.0
54	176280.5
55	168665.5
56	157390.0
57	144000.0
58	129580.5
59	112661.0
60	93715.5
61	77473.0
62	63666.5
63	49400.0
64	36065.0
65	26610.0
66	20113.0
67	15308.0
68	11585.0
69	8897.5
70	6696.5
71	4801.5
72	3406.0
73	2510.0
74	1924.0
75	1439.5
76	1100.5
77	724.5
78	438.0
79	320.5
80	238.0
81	165.5
82	132.5
83	116.5
84	97.0
85	87.5
86	76.0
87	60.5
88	44.0
89	42.5
90	44.0
91	34.0
92	26.5
93	22.5
94	19.0
95	18.5
96	18.5
97	12.0
98	7.0
99	6.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05098929604167768
2	0.05875403507183396
3	0.04990172673126492
4	0.05594658871239635
5	0.048687695873129724
6	0.018235755181572225
7	0.015150093417145303
8	0.020891447683742938
9	0.021119078469643286
10-11	0.022219293934828292
12-13	0.027720371260753343
14-15	0.04395803398831141
16-17	0.02985757141726215
18-19	0.023256278626152097
20-21	0.026658094259885058
22-23	0.04794157274156748
24-25	0.042389910796553465
26-27	0.029794340643400946
28-29	0.019070401396540163
30-31	0.012051785497946138
32-33	0.00918110836464732
34-35	0.008902892959658008
36-37	0.0013657847154020808
38-39	0.006487477398159884
40-41	0.0034397540980496844
42-43	0.22134564697854275
44-45	0.19556013739794237
46-47	0.20161764553384603
48-49	0.1995310299964262
50-51	0.1981652452810241
52-53	0.20304666102310934
54-55	0.20154176860521258
56-57	0.21170927704209477
58-59	0.20837069218222298
60-61	0.21268303095955732
62-63	0.2103814307910094
64-65	0.2119748462923118
66-67	0.20211084556996345
68-69	0.20082093778319485
70-71	0.18460856736518125
72-73	0.19238595255010976
74-75	0.19162718326377526
76-77	0.19012229084587853
78-79	0.21540195423558925
80-81	0.22343226251596263
82-83	0.22223087781259965
84-85	0.2175138620825536
86-87	0.23423207869145685
88-89	0.23273983242833232
90-91	0.23189254005859217
92-93	0.23593930958570944
94-95	0.24579066415328557
96-97	0.24663795652302575
98-99	0.23612900190729305
100-101	0.24572743337942438
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3953771.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.495773361050105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.00830497234416	40.876355879693946
2	12.963101629854886	14.128684969536623
3	4.703024010960299	7.68884791838608
4	2.202345981843872	4.8007418995673214
5	1.2871566819606581	3.5072299410144634
6	0.8444081958378908	2.761000659875695
7	0.5560913197479033	2.1213238570320287
8	0.4090367589817721	1.78326196110473
9	0.2970378172861289	1.4568575013437302
>10	1.6313027321112117	15.617186586057677
>50	0.07469729410838183	2.722932451564003
>100	0.022141537320192774	1.8768897493195096
>500	8.851822650630297E-4	0.3441703699057084
>1k	4.6588537763972155E-4	0.3145162555983819
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.058461908896595E-5
2	0.0	0.0	0.0	0.0	5.058461908896595E-5
3	0.0	0.0	0.0	0.0	5.058461908896595E-5
4	0.0	0.0	0.0	0.0	1.011692381779319E-4
5	0.0	2.5292309544482976E-5	0.0	0.0	1.011692381779319E-4
6	0.0	2.5292309544482976E-5	0.0	0.0	1.264615477224149E-4
7	0.0	2.5292309544482976E-5	0.0	0.0	1.264615477224149E-4
8	0.0	2.5292309544482976E-5	0.0	0.0	1.264615477224149E-4
9	0.0	2.5292309544482976E-5	0.0	0.0	1.5175385726689785E-4
10-11	0.0	2.5292309544482976E-5	0.0	0.0	1.5175385726689785E-4
12-13	0.0	3.793846431672446E-5	0.0	0.0	2.529230954448298E-4
14-15	0.0	7.587692863344892E-5	0.0	0.0	2.908615597615542E-4
16-17	0.0	1.011692381779319E-4	0.0	0.0	3.4144617885052014E-4
18-19	0.0	1.011692381779319E-4	0.0	0.0	3.5409233362276166E-4
20-21	0.0	1.011692381779319E-4	0.0	3.793846431672446E-5	3.920307979394861E-4
22-23	0.0	1.011692381779319E-4	0.0	1.1381539295017338E-4	4.6790772657293505E-4
24-25	0.0	1.011692381779319E-4	0.0	2.7821540498931276E-4	4.805538813451765E-4
26-27	0.0	1.011692381779319E-4	0.0	5.9436927429535E-4	4.805538813451765E-4
28-29	0.0	1.138153929501734E-4	0.0	0.0018716309062917402	4.805538813451765E-4
30-31	0.0	1.5175385726689785E-4	0.0	0.007246246684494372	4.805538813451765E-4
32-33	0.0	1.5175385726689785E-4	0.0	0.020550001504892418	5.311385004341425E-4
34-35	0.0	1.5175385726689785E-4	0.0	0.038026987400130156	5.817231195231085E-4
36-37	0.0	1.6440001203913934E-4	0.0	0.06789720497216455	5.943692742953499E-4
38-39	0.0	1.8969232158362232E-4	0.0	0.11550997768965375	6.070154290675914E-4
40-41	0.0	2.023384763558638E-4	0.0	0.17133010485432768	6.070154290675914E-4
42-43	0.0	2.023384763558638E-4	0.0	0.2305267553431901	6.070154290675914E-4
44-45	0.0	2.023384763558638E-4	0.0	0.30422854535581345	6.196615838398329E-4
46-47	0.0	2.023384763558638E-4	0.0	0.3939783057744113	6.702462029287989E-4
48-49	0.0	2.023384763558638E-4	0.0	0.4878507126487599	6.828923577010404E-4
50-51	1.2646154772241488E-5	2.023384763558638E-4	0.0	0.5881473661474071	7.081846672455233E-4
52-53	2.5292309544482976E-5	2.023384763558638E-4	0.0	0.6941727277578797	7.587692863344893E-4
54-55	2.5292309544482976E-5	2.276307859003468E-4	0.0	0.8195340600151095	8.346462149679382E-4
56-57	2.5292309544482976E-5	2.276307859003468E-4	0.0	0.9610824703808086	8.599385245124211E-4
58-59	2.5292309544482976E-5	2.7821540498931276E-4	0.0	1.1158208201739555	8.852308340569041E-4
60-61	2.5292309544482976E-5	3.035077145337957E-4	0.0	1.280751970713529	9.105231436013872E-4
62-63	2.5292309544482976E-5	3.161538693060372E-4	0.0	1.451993552484451	9.358154531458701E-4
64-65	2.5292309544482976E-5	3.288000240782787E-4	0.0	1.6397510123879204	9.737539174625946E-4
66-67	2.5292309544482976E-5	3.288000240782787E-4	0.0	1.8621209979030147	9.86400072234836E-4
68-69	2.5292309544482976E-5	3.288000240782787E-4	0.0	2.1077219697347163	9.86400072234836E-4
70-71	2.5292309544482976E-5	3.5409233362276166E-4	0.0	2.3663611271365994	0.0010116923817793191
72-73	2.5292309544482976E-5	3.7938464316724464E-4	0.0	2.641528808825802	0.0011002154651850093
74-75	2.5292309544482976E-5	3.7938464316724464E-4	0.0	2.9322006762657726	0.001163446239046217
76-77	2.5292309544482976E-5	3.7938464316724464E-4	0.0	3.2633149466673714	0.0011887385485907
78-79	2.5292309544482976E-5	4.046769527117276E-4	0.0	3.634036973815631	0.0012266770129074243
80-81	2.5292309544482976E-5	4.046769527117276E-4	0.0	4.023108571538413	0.0012646154772241488
82-83	2.5292309544482976E-5	4.046769527117276E-4	0.0	4.4278487550240015	0.0013278462510853562
84-85	2.5292309544482976E-5	4.1732310748396914E-4	0.0	4.858526201947457	0.0013404924058575977
86-87	2.5292309544482976E-5	4.552615718006936E-4	0.0	5.3210593127421895	0.0013784308701743223
88-89	2.5292309544482976E-5	4.552615718006936E-4	0.0	5.8272469498107	0.0014290154892632881
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3130	0.0	48.821545	1
GTATCAA	5655	0.0	42.0441	1
TCAACGC	6625	0.0	34.60013	4
CAACGCA	6740	0.0	33.938927	5
ATCAACG	7030	0.0	32.67349	3
TATCAAC	7220	0.0	32.143536	2
AACGCAG	7245	0.0	31.693453	6
GTACATG	11735	0.0	28.672337	1
TACATGG	12225	0.0	26.74806	2
ACGCAGA	8805	0.0	25.327454	7
ACATGGG	12665	0.0	25.14333	3
CGCAGAG	9025	0.0	23.975222	8
CATGGGG	11405	0.0	23.55253	4
GCAGAGT	10805	0.0	19.103107	9
ATGGGGG	7210	0.0	19.088583	5
GAGTACA	8640	0.0	18.345634	1
GTATATA	1075	0.0	17.658405	1
AGAGTAC	8845	0.0	17.274403	10-11
GTATAGA	1105	0.0	17.178993	1
TAGACAG	2055	0.0	16.85862	5
>>END_MODULE