FastQCFastQC Report
Mon 15 Aug 2016
SRR2921403_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921403_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3953771
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30700.049.2321931
GTATCAA53350.041.604751
TCAACGC69600.031.1634944
CAACGCA71600.030.0838055
ATCAACG72450.029.93763
TATCAAC72550.029.8963382
AACGCAG76850.028.1484576
GTACATG113900.026.2474651
TACATGG119250.024.317672
ACATGGG123100.023.2486883
ACGCAGA96350.022.0565387
CATGGGG108300.021.29844
CGCAGAG98900.021.1994118
AGAGTAC93400.017.71388810-11
TATATCG2453.7290429E-617.467235
GCAGAGT114050.017.4662729
ATGGGGG69650.017.2039035
TAAGTAC26150.015.9945281
GTATAGC9400.015.6745221
CTTACAC19100.015.6547583
Produced by FastQC (version 0.11.5)