##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921403_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3953771
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.368337468204405	33.0	31.0	34.0	29.0	34.0
2	31.5027514238938	34.0	31.0	34.0	28.0	34.0
3	31.578483933439745	34.0	31.0	34.0	28.0	34.0
4	35.05834328796483	37.0	35.0	37.0	32.0	37.0
5	34.79213287770081	37.0	35.0	37.0	32.0	37.0
6	34.74352586429512	37.0	35.0	37.0	32.0	37.0
7	34.610829256423806	37.0	35.0	37.0	32.0	37.0
8	34.57291709610901	37.0	35.0	37.0	32.0	37.0
9	36.190677204117286	39.0	37.0	39.0	32.0	39.0
10-11	36.03881939545816	39.0	37.0	39.0	31.5	39.0
12-13	35.97000597657274	39.0	37.0	39.0	31.0	39.0
14-15	37.221368789441776	40.0	38.0	41.0	31.0	41.0
16-17	37.198377321296554	40.0	38.0	41.0	31.0	41.0
18-19	37.15380152264762	40.0	38.0	41.0	31.0	41.0
20-21	37.11143905400692	40.0	38.0	41.0	31.0	41.0
22-23	37.004664913572384	40.0	38.0	41.0	31.0	41.0
24-25	36.937080700930835	40.0	37.0	41.0	30.5	41.0
26-27	36.80188268364556	40.0	37.0	41.0	30.0	41.0
28-29	36.652580283481264	40.0	37.0	41.0	30.0	41.0
30-31	36.52198584591773	40.0	37.0	41.0	30.0	41.0
32-33	36.480418946873755	40.0	37.0	41.0	30.0	41.0
34-35	36.616968838104185	40.0	37.0	41.0	30.0	41.0
36-37	36.59845625859464	40.0	37.0	41.0	30.0	41.0
38-39	36.51381529178093	40.0	37.0	41.0	30.0	41.0
40-41	36.39671670412879	40.0	37.0	41.0	29.5	41.0
42-43	36.3015613448528	40.0	36.5	41.0	29.0	41.0
44-45	36.17233610646646	40.0	36.0	41.0	28.5	41.0
46-47	36.0265849994853	40.0	36.0	41.0	28.0	41.0
48-49	35.87627697709351	40.0	35.5	41.0	28.0	41.0
50-51	35.71774680425346	40.0	35.0	41.0	27.5	41.0
52-53	35.542345649254855	39.0	35.0	41.0	27.0	41.0
54-55	35.32847944405481	39.0	35.0	41.0	26.5	41.0
56-57	35.05638831889859	39.0	35.0	41.0	26.0	41.0
58-59	34.82630809928041	38.5	35.0	40.5	26.0	41.0
60-61	34.54207438923498	38.0	34.5	40.0	26.0	41.0
62-63	34.23643908562231	37.0	34.0	40.0	25.0	41.0
64-65	33.91374905122224	37.0	34.0	40.0	25.0	41.0
66-67	33.57085804918899	36.0	34.0	39.0	23.5	41.0
68-69	33.20695963423273	36.0	34.0	39.0	23.0	41.0
70-71	32.82781729139093	35.0	33.0	38.5	22.5	40.0
72-73	32.43539623311517	35.0	33.0	37.0	22.0	39.5
74-75	32.03456775822373	35.0	33.0	37.0	20.5	39.0
76-77	29.311268533255973	33.0	29.0	34.5	14.0	36.5
78-79	30.962377057244844	34.5	31.5	36.0	18.0	37.0
80-81	31.167693703049572	35.0	32.0	35.5	19.0	37.0
82-83	31.019658068208805	35.0	32.0	35.0	18.0	37.0
84-85	30.80305207357735	35.0	32.0	35.0	17.5	36.0
86-87	30.57035283530584	35.0	32.0	35.0	12.5	36.0
88-89	30.307828905619473	35.0	32.0	35.0	7.0	36.0
90-91	30.137610650692718	35.0	32.0	35.0	4.5	35.0
92-93	30.006358992465675	34.0	31.0	35.0	2.0	35.0
94-95	29.922219698611784	34.0	31.0	35.0	2.0	35.0
96-97	29.73997318003496	34.0	31.0	35.0	2.0	35.0
98-99	29.489234707827034	34.0	31.0	35.0	2.0	35.0
100-101	28.414473422967593	33.5	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	80507.0
3	26629.0
4	13972.0
5	9785.0
6	8517.0
7	8625.0
8	8877.0
9	9517.0
10	10312.0
11	11167.0
12	11476.0
13	12598.0
14	13759.0
15	14947.0
16	15345.0
17	16419.0
18	15634.0
19	14242.0
20	13547.0
21	13446.0
22	14349.0
23	16295.0
24	19209.0
25	23105.0
26	28284.0
27	33988.0
28	41071.0
29	49341.0
30	60326.0
31	75777.0
32	96243.0
33	128209.0
34	185456.0
35	279244.0
36	495230.0
37	971338.0
38	988769.0
39	118215.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.97818278352908	19.274221902937096	13.799120907625387	25.94847440590844
2	15.03085041941142	20.40384394076011	37.70855806695154	26.85674757287692
3	17.914149720224994	24.280985062984158	28.906138153864365	28.89872706292649
4	13.270744184940664	16.658631823946394	34.95556217678307	35.11506181432988
5	14.569262825118551	37.12147507837657	33.89247686145613	14.416785235048746
6	34.07970045493761	35.01380595382233	16.67886190479924	14.227631686440825
7	29.466242935531067	31.55817303253987	20.431512747975663	18.544071283953397
8	27.197734171333813	34.939804441260925	19.199018032995447	18.663443354409807
9	26.80428380905217	15.641522991594606	18.88255541355329	38.67163778579993
10-11	26.069492534532134	25.211662847943415	27.235377290237018	21.483467327287435
12-13	26.455804831757533	22.362458703845807	27.305161317409915	23.876575146986738
14-15	23.63966675624832	23.96666297801034	24.341615947642158	28.052054318099184
16-17	24.105908725434478	27.13584501650706	25.05566216285599	23.702584095202468
18-19	24.188471346560835	26.586013431526844	26.213557609438613	23.011957612473704
20-21	24.61189600076181	25.619195291142656	26.21918408656309	23.549724621532445
22-23	24.52084254923727	25.293612692515964	25.967047089064266	24.218497669182497
24-25	24.31043932627381	25.56327431960792	25.85894575554675	24.267340598571522
26-27	24.09717526327817	25.834249045915804	25.917033088703494	24.151542602102534
28-29	24.247838287966815	26.037065942350385	25.71401217876266	24.001083590920143
30-31	24.61597459186135	25.71477464644974	25.5485304833033	24.120720278385615
32-33	24.323951380782784	25.74661784390181	25.531564787658027	24.397865987657376
34-35	24.545536524975052	25.85624648475438	25.621671227606296	23.976545762664276
36-37	24.58424507520282	25.693245246306567	25.739581103114606	23.982928575376008
38-39	24.49323929131338	25.765874522875603	25.621075812684342	24.119810373126676
40-41	24.586744537068007	25.59963729052991	25.64307895823466	24.170539214167423
42-43	24.41080675275972	25.612103700692728	25.522371618305144	24.454717928242413
44-45	24.333211836598103	25.65679693291989	25.62324410447842	24.38674712600358
46-47	24.507836397480556	25.590319347014518	25.49770312269582	24.40414113280911
48-49	24.29837157906266	25.681822378500808	25.57978205258896	24.44002398984757
50-51	24.377636863990933	25.679375044263903	25.660543914851424	24.282444176893737
52-53	24.58134506039717	25.554786796733747	25.561400848596765	24.30246729427232
54-55	24.118355919357782	25.494446960349272	25.791368694238653	24.595828426054297
56-57	24.237762136940834	25.63803902806586	25.573147808438492	24.551051026554813
58-59	24.31249532839945	25.57618936262484	25.677039021634364	24.434276287341344
60-61	24.37577495369881	25.581793880907416	25.78484376478135	24.257587400612426
62-63	24.311217891354406	25.60681442899307	25.73085686929124	24.35111081036128
64-65	24.135913956295283	25.442304045597975	25.719106585992783	24.702675412113962
66-67	24.148473803339463	25.62123422530358	25.60324503703344	24.627046934323523
68-69	24.099986389112505	25.547699076938958	25.65973590830411	24.69257862564443
70-71	24.095463820070968	25.411941795525255	25.629760716736467	24.862833667667307
72-73	24.086779925153518	25.645147722823875	25.461491123601686	24.806581228420917
74-75	24.021397395655125	25.73836257302544	25.47811782355244	24.762122207766993
76-77	24.093788169106215	25.363067232137254	25.593709255915158	24.94943534284137
78-79	23.93711206298715	25.427597577136886	25.66985098561691	24.965439374259056
80-81	24.0948918870701	25.58331888790899	25.47895755069711	24.842831674323797
82-83	24.060779385704727	25.568361630514662	25.55180452240522	24.81905446137539
84-85	24.023638398038692	25.601311079724248	25.461183425290656	24.9138670969464
86-87	23.8507631793643	25.79257428085922	25.426849626871245	24.929812912905234
88-89	23.698892429720175	25.623532105793494	25.52512621528612	25.152449249200203
90-91	23.7184240603671	25.505286713648683	25.603062656656228	25.173226569327987
92-93	23.796698036037093	25.421539226938627	25.620631840368592	25.16113089665569
94-95	23.72881398802579	25.387461975932457	25.620630306976093	25.263093729065663
96-97	23.5542699028387	25.29827366468823	25.67987171465795	25.46758471781512
98-99	23.34909907631294	25.355020082352826	25.611841506227044	25.68403933510719
100-101	23.366227749856748	25.414126805693034	25.583777567932703	25.635867876517516
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	71.0
1	52.5
2	58.0
3	84.0
4	68.0
5	72.0
6	139.0
7	287.0
8	464.5
9	515.5
10	422.0
11	355.0
12	356.0
13	361.0
14	396.0
15	479.5
16	550.5
17	579.5
18	623.5
19	701.0
20	816.5
21	963.5
22	1141.0
23	1329.0
24	1656.0
25	2148.0
26	2845.0
27	4076.0
28	6020.0
29	8853.0
30	13131.0
31	19124.5
32	27061.5
33	34007.0
34	40078.5
35	47942.0
36	58494.0
37	69766.0
38	80462.5
39	90603.0
40	100140.5
41	109313.0
42	118133.5
43	128467.5
44	139242.0
45	149788.5
46	158228.5
47	166361.0
48	174705.5
49	178885.5
50	179545.0
51	179958.5
52	179705.0
53	179006.5
54	176535.0
55	168786.5
56	157656.0
57	143410.0
58	128184.0
59	111332.5
60	92339.0
61	76317.0
62	62401.0
63	47510.0
64	34182.0
65	25002.5
66	18692.0
67	14283.5
68	10831.5
69	8077.0
70	5892.5
71	4200.0
72	2943.5
73	2114.0
74	1587.0
75	1113.5
76	754.5
77	449.0
78	231.0
79	114.5
80	75.5
81	53.5
82	30.5
83	20.5
84	16.5
85	12.5
86	6.0
87	3.0
88	3.0
89	2.5
90	2.0
91	2.0
92	2.5
93	1.0
94	1.0
95	0.5
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1533472727682003
2	4.046769527117276E-4
3	0.0059942773620424656
4	0.003970892598483827
5	0.026784555807607472
6	0.001062277000868285
7	1.5175385726689787E-4
8	0.00187163090629174
9	0.0
10-11	0.10502631538346556
12-13	3.288000240782787E-4
14-15	0.0052734465400247005
16-17	2.023384763558638E-4
18-19	3.414461788505202E-4
20-21	7.714154411067307E-4
22-23	2.023384763558638E-4
24-25	0.0014669539535800125
26-27	0.002794800204665369
28-29	0.006310431231348502
30-31	0.013898124094693395
32-33	0.014176339499682706
34-35	0.011520646997511996
36-37	7.461231315622478E-4
38-39	1.6440001203913934E-4
40-41	0.004894061896857456
42-43	0.008485569852174038
44-45	0.007688862101522824
46-47	0.009105231436013873
48-49	0.010926277723216646
50-51	0.0053366773138859075
52-53	0.001707230894252601
54-55	8.978769888291456E-4
56-57	0.005855169659547809
58-59	0.009143169900330596
60-61	0.00781532364924524
62-63	0.01741375512137653
64-65	0.03324674089622287
66-67	0.03533335643364272
68-69	0.026632801950340575
70-71	0.04135292610522966
72-73	0.0256337557233335
74-75	0.024116217150664518
76-77	0.24188300232866294
78-79	0.0693768050805168
80-81	0.053518526996125984
82-83	0.01987975530196362
84-85	0.03296852549123356
86-87	0.08240234449592554
88-89	0.26328029620329557
90-91	0.31912571567751397
92-93	0.05292415772183063
94-95	0.004792892658679524
96-97	0.0100284007343875
98-99	0.0012899077867686319
100-101	0.00153018472744122
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3953771.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.34615193942424
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.90327798812845	39.42455496376208
2	13.430211676250634	14.329002253197917
3	4.929163288195985	7.888556811190051
4	2.399321663847936	5.119783121247367
5	1.3399257713830288	3.573994189387464
6	0.8590381850406411	2.7495828924567167
7	0.5966930857886993	2.22818960109815
8	0.43121771497861083	1.840304459377625
9	0.3192588362938105	1.532810735004005
>10	1.6890652021800876	15.93696255489909
>50	0.07861247809820007	2.815808033721334
>100	0.022788205953993463	1.900345450439717
>500	0.0010931929776703611	0.4246284682736893
>1k	3.3271088241023684E-4	0.23547646594484256
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	2.5292309544482976E-5
5	0.0	2.5292309544482976E-5	0.0	0.0	2.5292309544482976E-5
6	0.0	2.5292309544482976E-5	0.0	0.0	7.587692863344892E-5
7	0.0	2.5292309544482976E-5	0.0	0.0	7.587692863344892E-5
8	0.0	2.5292309544482976E-5	0.0	0.0	7.587692863344892E-5
9	0.0	2.5292309544482976E-5	0.0	0.0	7.587692863344892E-5
10-11	0.0	2.5292309544482976E-5	0.0	0.0	7.587692863344892E-5
12-13	0.0	3.793846431672446E-5	0.0	0.0	2.529230954448298E-4
14-15	0.0	6.323077386120743E-5	0.0	0.0	3.4144617885052014E-4
16-17	0.0	7.587692863344892E-5	0.0	2.5292309544482976E-5	3.7938464316724464E-4
18-19	0.0	7.587692863344892E-5	0.0	5.058461908896595E-5	4.046769527117276E-4
20-21	0.0	1.011692381779319E-4	0.0	8.852308340569041E-5	4.299692622562106E-4
22-23	0.0	1.264615477224149E-4	0.0	1.6440001203913936E-4	4.805538813451766E-4
24-25	0.0	1.264615477224149E-4	0.0	3.667384883950032E-4	5.058461908896596E-4
26-27	0.0	1.264615477224149E-4	0.0	7.081846672455233E-4	5.058461908896596E-4
28-29	0.0	1.264615477224149E-4	0.0	0.0020360309183308795	5.058461908896596E-4
30-31	0.0	1.264615477224149E-4	0.0	0.007423292851305753	5.058461908896596E-4
32-33	0.0	1.264615477224149E-4	0.0	0.020891447683742938	5.564308099786255E-4
34-35	0.0	1.264615477224149E-4	0.0	0.03878575668646464	5.564308099786255E-4
36-37	0.0	1.5175385726689785E-4	0.0	0.06904800505643852	5.564308099786255E-4
38-39	0.0	1.8969232158362232E-4	0.0	0.11700222395277826	5.817231195231085E-4
40-41	0.0	2.023384763558638E-4	0.0	0.1728602895817689	6.070154290675914E-4
42-43	0.0	2.023384763558638E-4	0.0	0.2324489708685708	6.070154290675914E-4
44-45	0.0	2.023384763558638E-4	0.0	0.3070106994057066	6.070154290675914E-4
46-47	0.0	2.023384763558638E-4	0.0	0.39792390606335065	6.449538933843159E-4
48-49	0.0	2.276307859003468E-4	0.0	0.49237803605722236	7.208308220177648E-4
50-51	0.0	2.276307859003468E-4	0.0	0.592851735722681	7.334769767900064E-4
52-53	0.0	2.402769406725883E-4	0.0	0.6990162050356483	7.714154411067307E-4
54-55	0.0	3.035077145337957E-4	0.0	0.8243775372928781	7.840615958789722E-4
56-57	0.0	3.5409233362276166E-4	0.0	0.9660144707419829	7.967077506512137E-4
58-59	0.0	3.5409233362276166E-4	0.0	1.1202722666537843	8.346462149679382E-4
60-61	0.0	3.5409233362276166E-4	0.0	1.285519571062664	8.346462149679382E-4
62-63	0.0	3.5409233362276166E-4	0.0	1.4569761374647139	8.599385245124212E-4
64-65	0.0	3.5409233362276166E-4	0.0	1.644973874308856	8.599385245124212E-4
66-67	0.0	4.046769527117276E-4	0.0	1.867482967526445	8.599385245124212E-4
68-69	0.0	4.299692622562106E-4	0.0	2.1138680009540263	8.599385245124212E-4
70-71	0.0	4.4261541702845207E-4	0.0	2.373974112309489	8.978769888291457E-4
72-73	0.0	4.552615718006936E-4	0.0	2.650684624880905	0.0010116923817793191
74-75	0.0	4.552615718006936E-4	0.0	2.9447077233355197	0.0010116923817793191
76-77	0.0	4.552615718006936E-4	0.0	3.2784271016202	0.0010243385365515606
78-79	0.0	4.805538813451765E-4	0.0	3.652690052104687	0.001036984691323802
80-81	0.0	4.805538813451765E-4	0.0	4.0466810040338705	0.0010749231556405265
82-83	0.0	5.311385004341425E-4	0.0	4.45543001858226	0.001163446239046217
84-85	0.0	6.070154290675914E-4	0.0	4.890634788914179	0.001163446239046217
86-87	2.5292309544482976E-5	6.070154290675914E-4	0.0	5.358555161641886	0.0012013847033629415
88-89	2.5292309544482976E-5	6.070154290675914E-4	0.0	5.870420922203133	0.0012393231676796658
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3070	0.0	49.232193	1
GTATCAA	5335	0.0	41.60475	1
TCAACGC	6960	0.0	31.163494	4
CAACGCA	7160	0.0	30.083805	5
ATCAACG	7245	0.0	29.9376	3
TATCAAC	7255	0.0	29.896338	2
AACGCAG	7685	0.0	28.148457	6
GTACATG	11390	0.0	26.247465	1
TACATGG	11925	0.0	24.31767	2
ACATGGG	12310	0.0	23.248688	3
ACGCAGA	9635	0.0	22.056538	7
CATGGGG	10830	0.0	21.2984	4
CGCAGAG	9890	0.0	21.199411	8
AGAGTAC	9340	0.0	17.713888	10-11
TATATCG	245	3.7290429E-6	17.46723	5
GCAGAGT	11405	0.0	17.466272	9
ATGGGGG	6965	0.0	17.203903	5
TAAGTAC	2615	0.0	15.994528	1
GTATAGC	940	0.0	15.674522	1
CTTACAC	1910	0.0	15.654758	3
>>END_MODULE