FastQCFastQC Report
Mon 15 Aug 2016
SRR2921402_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921402_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2215162
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19850.053.556011
GTATCAA39950.043.954731
TCAACGC48500.035.5226364
ATCAACG50400.033.995923
CAACGCA50650.033.9179955
TATCAAC50750.033.6687052
AACGCAG53850.032.334326
GTACATG69100.026.7172831
ACGCAGA63800.026.625927
CGCAGAG64700.025.8900138
ACATGGG71700.024.8234563
TACATGG73300.024.7354222
CATGGGG57700.023.2782824
GCAGAGT72500.021.7301089
GAGTACA53150.020.2695121
GTATATA8300.020.0129281
AGAGTAC60750.019.25472810-11
ATGGGGG40250.019.1007425
GTATAAA9350.018.7806591
AGTACAT54100.018.6871172
Produced by FastQC (version 0.11.5)