##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921402_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2215162
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.265430248442325	31.0	31.0	34.0	26.0	34.0
2	30.32404447169101	31.0	31.0	34.0	26.0	34.0
3	30.371663110869545	33.0	31.0	34.0	26.0	34.0
4	33.829147033038666	37.0	35.0	37.0	29.0	37.0
5	33.82644610191038	37.0	35.0	37.0	30.0	37.0
6	33.86852203134579	37.0	35.0	37.0	30.0	37.0
7	33.78902626534764	37.0	35.0	37.0	29.0	37.0
8	33.75421255872031	37.0	35.0	37.0	29.0	37.0
9	35.294234010875954	39.0	35.0	39.0	29.0	39.0
10-11	35.18627982964677	39.0	35.0	39.0	28.5	39.0
12-13	35.068213069743884	39.0	35.0	39.0	27.0	39.0
14-15	36.18585480429874	40.0	36.0	41.0	27.0	41.0
16-17	36.13494678944475	40.0	36.0	41.0	27.0	41.0
18-19	36.094248637345714	40.0	36.0	41.0	27.0	41.0
20-21	36.03909465763678	40.0	36.0	41.0	27.0	41.0
22-23	35.923594527172284	40.0	36.0	41.0	27.0	41.0
24-25	35.828190218142055	40.0	36.0	41.0	27.0	41.0
26-27	35.680187724419255	40.0	36.0	41.0	26.5	41.0
28-29	35.53823151534741	40.0	36.0	41.0	25.5	41.0
30-31	35.38827792278849	40.0	35.5	41.0	25.0	41.0
32-33	35.20543147634349	39.0	35.0	41.0	24.5	41.0
34-35	35.043842843096805	39.0	35.0	41.0	24.0	41.0
36-37	34.87766470352959	39.0	35.0	41.0	24.0	41.0
38-39	35.09964914529954	39.0	35.0	41.0	24.0	41.0
40-41	35.21960380324329	40.0	35.0	41.0	24.0	41.0
42-43	35.05269321160259	40.0	35.0	41.0	23.0	41.0
44-45	34.929246935438584	40.0	35.0	41.0	22.5	41.0
46-47	34.77225525717758	40.0	35.0	41.0	21.5	41.0
48-49	34.61984134794656	39.0	35.0	41.0	19.5	41.0
50-51	32.9163332072327	37.0	32.5	39.5	15.5	40.0
52-53	33.071781657504054	37.5	32.5	39.5	14.5	40.0
54-55	33.936021609254766	39.0	34.0	40.5	14.5	41.0
56-57	33.96369610890761	39.0	34.0	41.0	13.5	41.0
58-59	33.79314628004634	38.0	34.0	41.0	13.0	41.0
60-61	33.55722290288475	38.0	34.0	40.0	9.5	41.0
62-63	33.27611366572738	37.5	33.5	40.0	8.0	41.0
64-65	32.99384898260263	37.0	33.0	40.0	7.0	41.0
66-67	32.663185581912295	36.5	33.0	39.5	2.0	41.0
68-69	32.30993241126383	36.0	33.0	39.0	2.0	41.0
70-71	31.93201851602727	35.0	32.0	39.0	2.0	41.0
72-73	31.58145092774253	35.0	32.0	38.0	2.0	40.0
74-75	31.189339876722336	35.0	32.0	37.0	2.0	39.5
76-77	30.81051363286297	35.0	31.5	37.0	2.0	39.0
78-79	30.468410436798756	35.0	31.0	36.0	2.0	39.0
80-81	30.118592455089065	35.0	31.0	36.0	2.0	37.0
82-83	29.81954750939209	35.0	31.0	35.0	2.0	37.0
84-85	29.581894913329137	35.0	31.0	35.0	2.0	36.5
86-87	29.355846660424838	35.0	31.0	35.0	2.0	36.0
88-89	29.158732408735794	35.0	30.5	35.0	2.0	36.0
90-91	28.912082502318114	34.5	30.0	35.0	2.0	36.0
92-93	28.725922979899437	34.0	30.0	35.0	2.0	35.0
94-95	28.527432756611027	34.0	30.0	35.0	2.0	35.0
96-97	28.28698442822692	34.0	30.0	35.0	2.0	35.0
98-99	28.07160943533701	34.0	29.5	35.0	2.0	35.0
100-101	27.045930952228325	33.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	82380.0
3	13818.0
4	9343.0
5	6946.0
6	6270.0
7	6562.0
8	6894.0
9	7247.0
10	7967.0
11	8420.0
12	8592.0
13	8819.0
14	9435.0
15	10311.0
16	10621.0
17	11493.0
18	10323.0
19	9316.0
20	9253.0
21	9664.0
22	10896.0
23	12281.0
24	14336.0
25	16585.0
26	19573.0
27	23313.0
28	27448.0
29	33047.0
30	39110.0
31	47531.0
32	59220.0
33	76485.0
34	108047.0
35	154392.0
36	252622.0
37	460909.0
38	507345.0
39	98328.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.08818348524404	18.959178327205716	13.663110541006857	24.28952764654339
2	15.652158594447316	19.677005828765118	40.09651972493143	24.574315851856134
3	19.130294179152408	24.174242872479912	29.562130006472277	27.133332941895404
4	13.219461627583964	15.851564776107269	37.045162485122376	33.88381111118639
5	14.045478955357963	37.61324686246516	34.39836609262813	13.942908089548748
6	33.11151827874648	35.47015068914979	17.34957047225927	14.068760559844462
7	28.62404151656089	32.22405181081863	21.088464245740187	18.063442426880293
8	26.655510874188487	35.04059703056043	19.87132308120219	18.432569014048894
9	26.965576282773153	15.84105975814266	19.441060480590096	37.75230347849409
10-11	25.611326831688956	25.45238670944504	27.880242230164715	21.05604422870129
12-13	26.083667016248203	22.891916086884372	27.831288714085577	23.193128182781848
14-15	23.278708514832704	24.49285180392874	25.103209066952097	27.125230614286455
16-17	23.39110844585655	27.66122557355907	25.9107448794449	23.036921101139487
18-19	23.41272887363692	26.990743458334276	27.280224866231684	22.316302801797125
20-21	23.807118724582917	26.15738880153747	27.352414978365847	22.68307749551377
22-23	23.726765834934987	25.906665664008948	27.051434308643792	23.315134192412273
24-25	23.516523219670923	26.113743701259917	26.9324698287104	23.437263250358757
26-27	23.30750113626896	26.3435801876238	26.968330552776504	23.380588123330735
28-29	23.390678766988138	26.641718295016	26.76193735785366	23.205665580142202
30-31	23.855983110696542	26.203432852061027	26.692440673206335	23.248143364036096
32-33	23.501616737864534	26.285444302680776	26.602087447414256	23.610851512040433
34-35	23.815202733055315	26.400647958402807	26.672459104172226	23.111690204369655
36-37	23.74489477606988	26.28785720767105	26.728146797506064	23.239101218753007
38-39	23.689820547976744	26.42509935571717	26.62162119456204	23.26345890174405
40-41	23.815375447498745	26.166318298579288	26.688035249132092	23.330271004789875
42-43	23.55812958743534	26.247419364397395	26.433761216872465	23.7606898312948
44-45	23.519470865896714	26.297479182011287	26.63535667094903	23.547693281142973
46-47	23.71322988965857	26.253231201362365	26.380421237841293	23.653117671137768
48-49	23.490980916115127	26.416930847433402	26.386197076967406	23.70589115948407
50-51	23.587056616335406	26.40319645112151	26.533977218863185	23.4757697136799
52-53	23.831516042165767	26.213500672027962	26.446103990532126	23.508879295274138
54-55	23.285421771159797	26.155253182105398	26.80892984193165	23.75039520480316
56-57	23.406059945341386	26.320884471647343	26.516643102499494	23.75641248051178
58-59	23.582206553147294	26.25033699367266	26.6064872865179	23.560969166662144
60-61	23.607940794622298	26.271187590755492	26.769715146498026	23.35115646812418
62-63	23.678094834424147	26.152940056231987	26.753528974445906	23.415436134897963
64-65	23.304852145792136	26.05066269255449	26.791194678646303	23.853290483007076
66-67	23.385088363644257	26.174292041482428	26.616856577338087	23.823763017535228
68-69	23.449835530965295	26.107144028287088	26.735733484402864	23.70728695634475
70-71	23.40536472903659	25.99307724381325	26.661520274884253	23.940037752265905
72-73	23.328359957252182	26.293503661288213	26.515157339739776	23.862979041719825
74-75	23.304215096558274	26.311654832436343	26.62889512007598	23.755234950929403
76-77	23.35831207927652	25.93104979701707	26.657867310013156	24.052770813693257
78-79	23.337559246664036	26.010289213064063	26.69280625902625	23.959345281245653
80-81	23.528616055574027	26.20823974104059	26.42600723403389	23.837136969351494
82-83	23.511334206926723	26.126233113713493	26.62059830063239	23.74183437872739
84-85	23.400150190905308	26.24316902810199	26.522424587879772	23.83425619311293
86-87	23.24362319115993	26.350629242420542	26.508041652074688	23.897705914344844
88-89	23.203634469912057	26.09845573034847	26.629002914254148	24.06890688548533
90-91	23.342196848769152	25.992044101305595	26.703772174624373	23.96198687530088
92-93	23.531703379502364	25.826663773891134	26.786075855723503	23.855556990883006
94-95	23.39426718172297	25.844269003109027	26.792159034832817	23.969304780335186
96-97	23.28232875553144	25.654326708349416	26.900894940736254	24.16244959538289
98-99	23.071368117991923	25.59122766176029	26.8555464080882	24.481857812159586
100-101	23.3058175851605	25.591523725658245	26.93801852222241	24.16464016695884
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	75.0
1	62.5
2	76.0
3	102.0
4	99.0
5	116.5
6	177.0
7	358.0
8	545.5
9	615.0
10	577.0
11	517.5
12	539.0
13	598.0
14	652.5
15	736.5
16	846.5
17	941.5
18	1035.5
19	1151.5
20	1317.5
21	1488.5
22	1634.0
23	1894.0
24	2267.5
25	2784.0
26	3581.5
27	4955.5
28	6845.5
29	9241.0
30	12705.0
31	17419.0
32	23214.0
33	28194.0
34	32595.0
35	37803.5
36	44414.0
37	50861.5
38	56313.0
39	61617.0
40	65884.5
41	69657.5
42	73297.5
43	77387.0
44	81836.0
45	86443.0
46	89499.0
47	91681.0
48	93852.5
49	94476.5
50	93723.0
51	92403.5
52	90662.0
53	88581.0
54	86623.5
55	82656.0
56	76429.0
57	68721.0
58	61139.0
59	52477.0
60	42633.0
61	35076.0
62	28557.5
63	21577.0
64	15413.5
65	11110.0
66	8244.5
67	6232.0
68	4669.0
69	3540.5
70	2646.0
71	1918.5
72	1373.5
73	995.0
74	759.5
75	552.0
76	379.5
77	250.0
78	182.5
79	135.0
80	85.5
81	64.0
82	62.5
83	53.5
84	37.0
85	37.0
86	37.0
87	30.0
88	22.0
89	18.5
90	19.0
91	17.0
92	11.5
93	11.0
94	8.5
95	10.0
96	10.5
97	5.5
98	6.0
99	5.5
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05155379155113712
2	0.05931846068143097
3	0.04979319796926816
4	0.057196719698153
5	0.04889032946574562
6	0.018237943771155337
7	0.01512304743400257
8	0.02112712298242747
9	0.02112712298242747
10-11	0.022187993474066456
12-13	0.02751491764484945
14-15	0.04365369214531488
16-17	0.02961408691553936
18-19	0.02311343369017706
20-21	0.027447202507085263
22-23	0.04724259444681698
24-25	0.04196081370121011
26-27	0.03058467055682609
28-29	0.02054025845513782
30-31	0.012143581372378183
32-33	0.009389832436634432
34-35	0.00925440216110605
36-37	0.0013091593301076852
38-39	0.0063652229498339175
40-41	0.0032503266126811497
42-43	0.2125578174417943
44-45	0.1875032164690438
46-47	0.19398129798181804
48-49	0.19136297932160268
50-51	0.19005381999149498
52-53	0.1948615947727525
54-55	0.19334929002935228
56-57	0.20456743118561985
58-59	0.20034652093165198
60-61	0.20549287140173042
62-63	0.20307769815480764
64-65	0.20411599693385857
66-67	0.19402644140699415
68-69	0.19228841953771328
70-71	0.1762399318876001
72-73	0.18305658908919528
74-75	0.18337259306542816
76-77	0.18127342379473826
78-79	0.2074114669717158
80-81	0.21565014206635902
82-83	0.2144989847243678
84-85	0.20955577966758188
86-87	0.22770343658838496
88-89	0.22531083505405022
90-91	0.22472397052676057
92-93	0.22835801625343882
94-95	0.23955358569711832
96-97	0.23903443630759286
98-99	0.22926088475696133
100-101	0.23817671122924644
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2215162.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.00174612517239
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.17268409229385	50.51019089356345
2	11.205737782767233	14.119620938703067
3	3.536739421899925	6.684622775082842
4	1.63086245749158	4.10988730047837
5	0.903692037418659	2.846708815839507
6	0.5796681853169086	2.1912064708905152
7	0.3951825196229849	1.7428032142075274
8	0.2696443185348038	1.3590450320339857
9	0.2137775751098137	1.2121524462891111
>10	1.034424317043235	11.209654299637185
>50	0.0438411839067483	1.8461101624814038
>100	0.0120929876926065	1.3346657767941474
>500	0.001437496436115656	0.64658728624849
>1k	2.1562446541723173E-4	0.18674458775038416
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.514342517612707E-5	4.514342517612707E-5
2	0.0	0.0	0.0	9.028685035225414E-5	4.514342517612707E-5
3	0.0	0.0	0.0	9.028685035225414E-5	4.514342517612707E-5
4	0.0	0.0	0.0	9.028685035225414E-5	4.514342517612707E-5
5	0.0	0.0	0.0	9.028685035225414E-5	4.514342517612707E-5
6	0.0	0.0	0.0	9.028685035225414E-5	9.028685035225414E-5
7	0.0	0.0	0.0	9.028685035225414E-5	9.028685035225414E-5
8	0.0	0.0	0.0	9.028685035225414E-5	9.028685035225414E-5
9	0.0	0.0	0.0	9.028685035225414E-5	9.028685035225414E-5
10-11	0.0	0.0	0.0	9.028685035225414E-5	9.028685035225414E-5
12-13	0.0	0.0	0.0	9.028685035225414E-5	1.3543027552838122E-4
14-15	0.0	0.0	0.0	9.028685035225414E-5	1.3543027552838122E-4
16-17	0.0	0.0	0.0	9.028685035225414E-5	1.3543027552838122E-4
18-19	0.0	0.0	0.0	9.028685035225414E-5	1.8057370070450829E-4
20-21	0.0	0.0	0.0	1.1285856294031768E-4	1.8057370070450829E-4
22-23	0.0	0.0	0.0	1.3543027552838122E-4	2.482888384686989E-4
24-25	0.0	0.0	0.0	2.7086055105676244E-4	2.7086055105676244E-4
26-27	0.0	0.0	0.0	6.997230902299696E-4	3.1600397623288953E-4
28-29	0.0	0.0	0.0	0.0024377449595108618	3.1600397623288953E-4
30-31	0.0	0.0	0.0	0.008441820507935762	3.3857568882095305E-4
32-33	0.0	0.0	0.0	0.023000575127236744	3.6114740140901657E-4
34-35	0.0	0.0	0.0	0.041622238012389165	4.5143425176127075E-4
36-37	0.0	0.0	0.0	0.07631496026024281	4.5143425176127075E-4
38-39	0.0	0.0	0.0	0.12856847490160989	4.5143425176127075E-4
40-41	0.0	0.0	0.0	0.18831579812221408	4.5143425176127075E-4
42-43	0.0	0.0	0.0	0.24971085636174692	4.5143425176127075E-4
44-45	0.0	0.0	0.0	0.32564209750799267	4.7400596434933433E-4
46-47	0.0	0.0	0.0	0.4152743681951929	5.417211021135249E-4
48-49	0.0	0.0	0.0	0.5099401307895315	6.320079524657791E-4
50-51	0.0	0.0	0.0	0.6135668632813311	6.545796650538426E-4
52-53	0.0	0.0	0.0	0.7191799064808804	7.674382279941603E-4
54-55	0.0	0.0	0.0	0.8446786284705137	9.028685035225415E-4
56-57	0.0	0.0	0.0	0.986948132913078	9.028685035225415E-4
58-59	0.0	0.0	0.0	1.1367340176474676	9.028685035225415E-4
60-61	0.0	0.0	0.0	1.3023426729060898	9.028685035225415E-4
62-63	0.0	0.0	0.0	1.4765060072355882	9.028685035225415E-4
64-65	0.0	0.0	0.0	1.6598786003010164	9.028685035225415E-4
66-67	0.0	0.0	0.0	1.8768830451226592	9.480119286986687E-4
68-69	0.0	0.0	0.0	2.109981121019591	9.931553538747957E-4
70-71	0.0	0.0	0.0	2.363619455371661	0.0010382987790509227
72-73	0.0	0.0	0.0	2.6285662177303513	0.0011511573419912403
74-75	0.0	0.0	0.0	2.9087488860859834	0.0011737290545793038
76-77	0.0	0.0	0.0	3.2234662747013534	0.0011737290545793038
78-79	0.0	0.0	0.0	3.579061034813707	0.0011737290545793038
80-81	0.0	0.0	0.0	3.9533903163741524	0.0011963007671673674
82-83	0.0	0.0	0.0	4.33947946019298	0.0012188724797554309
84-85	0.0	0.0	0.0	4.752022651165016	0.0012188724797554309
86-87	0.0	0.0	0.0	5.194428217891062	0.0012188724797554309
88-89	0.0	0.0	0.0	5.67281309448248	0.0012188724797554309
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1985	0.0	53.55601	1
GTATCAA	3995	0.0	43.95473	1
TCAACGC	4850	0.0	35.522636	4
ATCAACG	5040	0.0	33.99592	3
CAACGCA	5065	0.0	33.917995	5
TATCAAC	5075	0.0	33.668705	2
AACGCAG	5385	0.0	32.33432	6
GTACATG	6910	0.0	26.717283	1
ACGCAGA	6380	0.0	26.62592	7
CGCAGAG	6470	0.0	25.890013	8
ACATGGG	7170	0.0	24.823456	3
TACATGG	7330	0.0	24.735422	2
CATGGGG	5770	0.0	23.278282	4
GCAGAGT	7250	0.0	21.730108	9
GAGTACA	5315	0.0	20.269512	1
GTATATA	830	0.0	20.012928	1
AGAGTAC	6075	0.0	19.254728	10-11
ATGGGGG	4025	0.0	19.100742	5
GTATAAA	935	0.0	18.780659	1
AGTACAT	5410	0.0	18.687117	2
>>END_MODULE