FastQCFastQC Report
Mon 15 Aug 2016
SRR2921402_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921402_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2215162
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21550.056.6706121
GTATCAA39950.042.9400441
TCAACGC52350.032.1857534
ATCAACG53900.031.43633
TATCAAC54200.031.0871622
CAACGCA54200.030.9644625
AACGCAG57600.029.1327466
GTACATG66200.025.6978681
TACATGG66950.024.812442
ACGCAGA68050.024.3091897
CGCAGAG70100.023.1914278
ACATGGG71750.022.4910133
GTATCGC1100.002865322221.60766
CATGGGG58300.020.3527664
GCAGAGT78500.019.1353659
AGAGTAC66600.019.02139510-11
ATGGGGG37700.018.1597235
CAGAGTA71700.017.2706210-11
GTGGTAT11600.016.7956921
GTATTAG4851.8189894E-1216.6563281
Produced by FastQC (version 0.11.5)