##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921402_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2215162
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.361772637847707	33.0	31.0	34.0	28.0	34.0
2	31.493394162593976	34.0	31.0	34.0	28.0	34.0
3	31.568015341541614	34.0	31.0	34.0	28.0	34.0
4	35.057607073433005	37.0	35.0	37.0	32.0	37.0
5	34.79581944796814	37.0	35.0	37.0	32.0	37.0
6	34.75633745974335	37.0	35.0	37.0	32.0	37.0
7	34.62252512457328	37.0	35.0	37.0	32.0	37.0
8	34.583260727657844	37.0	35.0	37.0	32.0	37.0
9	36.19314208170779	39.0	37.0	39.0	32.0	39.0
10-11	36.04226914329516	39.0	37.0	39.0	31.5	39.0
12-13	35.97366919439752	39.0	37.0	39.0	31.0	39.0
14-15	37.205291757442566	40.0	38.0	41.0	31.0	41.0
16-17	37.189495170104934	40.0	38.0	41.0	31.0	41.0
18-19	37.16034876907423	40.0	38.0	41.0	31.0	41.0
20-21	37.124608719362286	40.0	38.0	41.0	31.0	41.0
22-23	37.017658979343274	40.0	38.0	41.0	31.0	41.0
24-25	36.952179343993805	40.0	37.0	41.0	30.5	41.0
26-27	36.81407522339224	40.0	37.0	41.0	30.0	41.0
28-29	36.661507826515624	40.0	37.0	41.0	30.0	41.0
30-31	36.525599933548875	40.0	37.0	41.0	30.0	41.0
32-33	36.4680716353928	40.0	36.5	41.0	30.0	41.0
34-35	36.58931694386234	40.0	37.0	41.0	30.0	41.0
36-37	36.562945734894335	40.0	37.0	41.0	30.0	41.0
38-39	36.476733078664225	40.0	37.0	41.0	30.0	41.0
40-41	36.352928815138576	40.0	37.0	41.0	29.5	41.0
42-43	36.24330951867177	40.0	36.0	41.0	29.0	41.0
44-45	36.10232569897822	40.0	36.0	41.0	28.0	41.0
46-47	35.94066145049437	40.0	36.0	41.0	28.0	41.0
48-49	35.78313865983617	40.0	35.5	41.0	27.0	41.0
50-51	35.63155877538527	40.0	35.0	41.0	27.0	41.0
52-53	35.46402859023404	39.0	35.0	41.0	26.5	41.0
54-55	35.260293829525786	39.0	35.0	41.0	26.0	41.0
56-57	35.00318238575779	39.0	35.0	41.0	26.0	41.0
58-59	34.7799364109713	38.5	35.0	40.5	25.5	41.0
60-61	34.5102197943085	38.0	34.5	40.0	24.5	41.0
62-63	34.21765812161819	37.5	34.0	40.0	24.0	41.0
64-65	33.91109747278077	37.0	34.0	40.0	23.0	41.0
66-67	33.575435114903556	36.5	34.0	39.5	22.5	41.0
68-69	33.21357241592263	36.0	34.0	39.0	22.0	41.0
70-71	32.831287959977644	35.5	33.0	39.0	21.5	40.0
72-73	32.43430480479532	35.0	33.0	37.5	20.0	40.0
74-75	32.02303533556462	35.0	33.0	37.0	18.5	39.0
76-77	29.27142552102284	33.0	29.0	34.5	11.0	36.5
78-79	30.91957766520011	34.5	31.5	36.0	14.5	38.0
80-81	31.110437295330996	35.0	32.0	36.0	16.5	37.0
82-83	30.938251694458465	35.0	32.0	35.0	13.5	37.0
84-85	30.702727385175443	35.0	32.0	35.0	9.5	36.5
86-87	30.456565027749665	35.0	32.0	35.0	7.0	36.0
88-89	30.174553147805895	35.0	32.0	35.0	2.0	36.0
90-91	29.986616103020907	34.5	31.0	35.0	2.0	35.5
92-93	29.84130032024746	34.0	31.0	35.0	2.0	35.0
94-95	29.740767943834356	34.0	31.0	35.0	2.0	35.0
96-97	29.55476619768667	34.0	31.0	35.0	2.0	35.0
98-99	29.29237658464708	34.0	31.0	35.0	2.0	35.0
100-101	28.208962820777895	33.0	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	43153.0
3	14573.0
4	7853.0
5	5493.0
6	4771.0
7	4700.0
8	4863.0
9	5258.0
10	5824.0
11	6564.0
12	6921.0
13	7593.0
14	8238.0
15	9385.0
16	10450.0
17	11387.0
18	10436.0
19	8837.0
20	8453.0
21	8141.0
22	8682.0
23	9627.0
24	11108.0
25	13374.0
26	16114.0
27	19385.0
28	23037.0
29	28353.0
30	34045.0
31	42666.0
32	54028.0
33	71406.0
34	103568.0
35	152328.0
36	262997.0
37	520487.0
38	571939.0
39	79124.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.26145179666166	20.349657305097928	14.22952420565311	25.159366692587305
2	14.850112068907142	21.10082590157348	38.394198148662284	25.6548638808571
3	17.697128611582887	25.274014842263703	29.450399227819446	27.57845731833396
4	12.898020950288775	17.150334551814336	35.93575413319681	34.01589036470008
5	13.875295825056705	37.958672771089994	34.32383457006758	13.842196833785717
6	33.03061219883258	36.23408106973406	16.940269871294237	13.795036860139135
7	28.5009532038529	32.41123331441823	20.993507921094675	18.094305560634194
8	26.690081394771415	35.65966782987906	19.51289540568725	18.137355369662277
9	26.706489186795366	15.921408908242377	19.30481833834275	38.06728356661951
10-11	25.547604737653135	25.83238812242703	27.74303970789594	20.876967432023896
12-13	26.022128454100546	23.0940515728682	27.838749127037044	23.04507084599421
14-15	23.15787644091284	24.71030504790985	25.084654017265105	27.047164493912202
16-17	23.330862476502784	27.942998145503072	25.780509363674614	22.945630014319534
18-19	23.29882633468681	27.24053784961726	27.21430943078688	22.246326384909043
20-21	23.707656796874964	26.277242597881944	27.357421168746214	22.657679436496878
22-23	23.605274117077453	25.945581747573254	27.125317274279592	23.323826861069698
24-25	23.464352334225985	26.28536822317848	26.972553229525754	23.277726213069787
26-27	23.23768313204468	26.52872722594101	26.977826082540197	23.25576355947411
28-29	23.338186562649547	26.79228629989797	26.770886944587406	23.098640192865076
30-31	23.756309401609453	26.358382364129028	26.695967378370405	23.18934085589111
32-33	23.502704152387007	26.388179342519724	26.604380598189163	23.504735906904106
34-35	23.704781757345827	26.532136708840188	26.717450066526794	23.04563146728719
36-37	23.710937403011496	26.418974997816168	26.818880827972325	23.051206771200007
38-39	23.615768734971425	26.530868826138843	26.612939813931796	23.240422624957933
40-41	23.796558351237426	26.32890196714014	26.646819045950803	23.227720635671634
42-43	23.57504631541071	26.345889604647997	26.448418955424113	23.630645124517176
44-45	23.492509302000627	26.377591134549494	26.652830577966714	23.477068985483168
46-47	23.618841058339417	26.411893124053453	26.367987149206503	23.60127866840063
48-49	23.445495499156177	26.574008608896584	26.407027257893972	23.573468634053267
50-51	23.564188769758697	26.58251306863046	26.45341815960636	23.39988000200448
52-53	23.903222660923127	26.355024499735002	26.28193610656911	23.45981673277276
54-55	23.199237067676385	26.295884566985116	26.719018571081932	23.785859794256563
56-57	23.388106377417078	26.431353410512504	26.451082360918797	23.729457851151615
58-59	23.526483798607504	26.41246512052086	26.460344337577148	23.600706743294484
60-61	23.613172505098774	26.43661321652331	26.640157923785296	23.310056354592625
62-63	23.60450492001796	26.32475437159969	26.612322631811715	23.458418076570638
64-65	23.22209294265263	26.19257016038369	26.671004920727135	23.914331976236543
66-67	23.303912406036346	26.367786353895166	26.500985502088987	23.827315737979504
68-69	23.352111938306955	26.278697918383507	26.52427529049566	23.84491485281388
70-71	23.317803606461425	26.125799820074352	26.51387739765374	24.04251917581048
72-73	23.319061354757256	26.45376402738919	26.261652238150884	23.965522379702662
74-75	23.21198726732145	26.44022113986778	26.31988427793254	24.02790731487822
76-77	23.18783753239146	26.0612055719755	26.503446679582897	24.247510216050145
78-79	23.188169455256567	26.14397611954862	26.489123268419952	24.178731156774866
80-81	23.464274833743872	26.284175749674173	26.183836021894525	24.067713394687438
82-83	23.30801589077205	26.303304963619954	26.390786837939995	23.997892307667996
84-85	23.28821096073331	26.336358827063673	26.233895901008804	24.14153431119422
86-87	23.105783104278586	26.475809925788475	26.205674648059457	24.21273232187348
88-89	23.01306630105587	26.212379558687005	26.322913757524542	24.451640382732585
90-91	23.092110343197206	26.090401388177796	26.378163417946272	24.439324850678727
92-93	23.22752350346765	25.977186449175377	26.420517290828737	24.37477275652824
94-95	23.06779841217637	25.952593728929603	26.40922112399999	24.57038673489404
96-97	22.905709316198124	25.783110771998697	26.547377854629882	24.7638020571733
98-99	22.708715504696432	25.754213562718537	26.41740008581856	25.119670846766468
100-101	22.728629103432894	25.825675006444328	26.437174933987983	25.008520956134795
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	57.0
1	49.5
2	61.5
3	83.0
4	78.5
5	81.0
6	130.5
7	309.0
8	493.0
9	525.5
10	446.5
11	379.0
12	379.0
13	385.5
14	422.5
15	477.5
16	543.0
17	625.5
18	718.5
19	814.0
20	909.0
21	1086.5
22	1325.0
23	1614.0
24	1975.5
25	2566.5
26	3472.5
27	4789.5
28	6740.0
29	9219.5
30	12661.0
31	17419.5
32	23677.5
33	28706.5
34	32695.0
35	38014.5
36	44698.5
37	51253.0
38	56690.0
39	61875.5
40	66087.5
41	69507.5
42	73773.0
43	78051.5
44	81978.5
45	86811.5
46	90029.5
47	92231.5
48	94525.0
49	95405.5
50	94751.5
51	93374.0
52	91885.0
53	89871.5
54	87780.0
55	83598.5
56	76756.0
57	68784.5
58	61045.5
59	52147.0
60	42085.5
61	34293.5
62	27843.5
63	20895.5
64	14670.0
65	10452.5
66	7635.0
67	5735.0
68	4208.5
69	3101.0
70	2312.5
71	1648.0
72	1120.5
73	790.5
74	558.5
75	374.5
76	256.0
77	154.5
78	83.0
79	45.5
80	27.5
81	17.5
82	11.5
83	5.0
84	3.5
85	2.5
86	2.5
87	4.0
88	2.5
89	2.0
90	2.5
91	1.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14816072142804906
2	3.1600397623288953E-4
3	0.0061395058239532814
4	0.004920633344197851
5	0.026770051129443355
6	0.0014445896056360663
7	4.514342517612707E-5
8	0.0014445896056360663
9	0.0
10-11	0.10166299349663817
12-13	2.2571712588063538E-4
14-15	0.005259209033018804
16-17	2.7086055105676244E-4
18-19	3.3857568882095305E-4
20-21	8.577250783464145E-4
22-23	2.0314541329257186E-4
24-25	0.001715450156692829
26-27	0.002686033797979561
28-29	0.0064103663750100445
30-31	0.01410732036753971
32-33	0.014784471745181616
34-35	0.011963007671673674
36-37	6.997230902299696E-4
38-39	2.93432263644826E-4
40-41	0.005372067595959122
42-43	0.0084192487953477
44-45	0.008193531669467064
46-47	0.009096400172989606
48-49	0.011014995742975006
50-51	0.005642928147015884
52-53	0.0016251633063405749
54-55	0.0011060139168151133
56-57	0.006455509800186172
58-59	0.00963812127510313
60-61	0.007651810567353539
62-63	0.017312503555044732
64-65	0.03401557087021175
66-67	0.03649845925489874
68-69	0.02640890372803434
70-71	0.04126109061098014
72-73	0.025551178649687922
74-75	0.024625738433577318
76-77	0.23842500006771516
78-79	0.06629311987114261
80-81	0.05342724369594638
82-83	0.018847380011033053
84-85	0.03171325618622926
86-87	0.0824544660841961
88-89	0.2659399177125646
90-91	0.31995402593580063
92-93	0.050876640173495205
94-95	0.005304352458194931
96-97	0.010360416077921163
98-99	0.0010608704916389862
100-101	0.0015800198811644476
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2215162.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.05858029406023
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.36658835656449	49.253777961914835
2	11.595412754036042	14.391897068782313
3	3.7672902341370906	7.0137805045867685
4	1.6614497519576943	4.124288513456522
5	0.9547038225715782	2.9623781915052256
6	0.567989025419291	2.1149155524076853
7	0.4071011462048394	1.7684883418689883
8	0.30453530125573647	1.5119222756283957
9	0.2226845451504972	1.243753805292157
>10	1.0957280723007035	11.629044966224573
>50	0.04199351721181154	1.7728801601529367
>100	0.012774278282319071	1.3526389086967623
>500	0.001311896180965563	0.5248063618093342
>1k	4.372987269881519E-4	0.335427387673477
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.514342517612707E-5	0.0
3	0.0	0.0	0.0	4.514342517612707E-5	0.0
4	0.0	0.0	0.0	4.514342517612707E-5	0.0
5	0.0	0.0	0.0	4.514342517612707E-5	0.0
6	0.0	0.0	0.0	4.514342517612707E-5	4.514342517612707E-5
7	0.0	0.0	0.0	4.514342517612707E-5	4.514342517612707E-5
8	4.514342517612707E-5	0.0	0.0	4.514342517612707E-5	4.514342517612707E-5
9	4.514342517612707E-5	0.0	0.0	4.514342517612707E-5	4.514342517612707E-5
10-11	4.514342517612707E-5	0.0	0.0	4.514342517612707E-5	4.514342517612707E-5
12-13	4.514342517612707E-5	0.0	0.0	4.514342517612707E-5	4.514342517612707E-5
14-15	4.514342517612707E-5	0.0	0.0	4.514342517612707E-5	9.028685035225414E-5
16-17	4.514342517612707E-5	0.0	0.0	4.514342517612707E-5	1.3543027552838122E-4
18-19	4.514342517612707E-5	0.0	0.0	4.514342517612707E-5	1.8057370070450829E-4
20-21	4.514342517612707E-5	0.0	0.0	6.771513776419061E-5	1.8057370070450829E-4
22-23	4.514342517612707E-5	0.0	0.0	9.028685035225414E-5	2.2571712588063535E-4
24-25	4.514342517612707E-5	0.0	0.0	2.2571712588063538E-4	2.7086055105676244E-4
26-27	4.514342517612707E-5	0.0	0.0	6.545796650538426E-4	2.7086055105676244E-4
28-29	4.514342517612707E-5	0.0	0.0	0.0023926015343347347	2.7086055105676244E-4
30-31	4.514342517612707E-5	0.0	0.0	0.008328961944995446	2.7086055105676244E-4
32-33	4.514342517612707E-5	0.0	0.0	0.023068290265000936	2.7086055105676244E-4
34-35	4.514342517612707E-5	0.0	2.2571712588063536E-5	0.041983385413798174	4.965776769373978E-4
36-37	4.514342517612707E-5	0.0	4.514342517612707E-5	0.07721782876376536	4.965776769373978E-4
38-39	4.514342517612707E-5	0.0	4.514342517612707E-5	0.12967448881842503	4.965776769373978E-4
40-41	4.514342517612707E-5	0.0	4.514342517612707E-5	0.1892186666257366	4.965776769373978E-4
42-43	4.514342517612707E-5	0.0	4.514342517612707E-5	0.25029772088903657	4.965776769373978E-4
44-45	4.514342517612707E-5	0.0	4.514342517612707E-5	0.32674811142480775	4.965776769373978E-4
46-47	4.514342517612707E-5	0.0	4.514342517612707E-5	0.41732839404070676	4.965776769373978E-4
48-49	4.514342517612707E-5	0.0	4.514342517612707E-5	0.5117007243714005	4.965776769373978E-4
50-51	4.514342517612707E-5	0.0	4.514342517612707E-5	0.6146277337729701	4.965776769373978E-4
52-53	4.514342517612707E-5	0.0	4.514342517612707E-5	0.7201730618347553	5.417211021135249E-4
54-55	4.514342517612707E-5	0.0	4.514342517612707E-5	0.8452203495726271	5.868645272896519E-4
56-57	4.514342517612707E-5	0.0	4.514342517612707E-5	0.9871061349011945	5.868645272896519E-4
58-59	4.514342517612707E-5	0.0	4.514342517612707E-5	1.1374337407376978	6.320079524657791E-4
60-61	4.514342517612707E-5	0.0	4.514342517612707E-5	1.3028843940082036	6.771513776419061E-4
62-63	4.514342517612707E-5	0.0	4.514342517612707E-5	1.4770703000502898	8.125816531702873E-4
64-65	4.514342517612707E-5	0.0	4.514342517612707E-5	1.6603074628401897	8.125816531702873E-4
66-67	4.514342517612707E-5	0.0	4.514342517612707E-5	1.876070463469489	8.125816531702873E-4
68-69	4.514342517612707E-5	0.0	4.514342517612707E-5	2.1090105373783046	8.125816531702873E-4
70-71	4.514342517612707E-5	0.0	4.514342517612707E-5	2.3637323139346016	8.125816531702873E-4
72-73	4.514342517612707E-5	0.0	4.514342517612707E-5	2.6304848133003365	9.028685035225415E-4
74-75	4.514342517612707E-5	0.0	4.514342517612707E-5	2.913511517442065	9.028685035225415E-4
76-77	4.514342517612707E-5	0.0	4.514342517612707E-5	3.231795236646349	9.028685035225415E-4
78-79	4.514342517612707E-5	0.0	4.514342517612707E-5	3.59032431939515	9.028685035225415E-4
80-81	4.514342517612707E-5	0.0	4.514342517612707E-5	3.9679716427060416	9.480119286986687E-4
82-83	4.514342517612707E-5	0.0	4.514342517612707E-5	4.358304268491424	9.931553538747957E-4
84-85	4.514342517612707E-5	0.0	4.514342517612707E-5	4.774752365741197	9.931553538747957E-4
86-87	4.514342517612707E-5	0.0	4.514342517612707E-5	5.222665430338729	9.931553538747957E-4
88-89	4.514342517612707E-5	0.0	4.514342517612707E-5	5.7068963804904556	9.931553538747957E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2155	0.0	56.670612	1
GTATCAA	3995	0.0	42.940044	1
TCAACGC	5235	0.0	32.185753	4
ATCAACG	5390	0.0	31.4363	3
TATCAAC	5420	0.0	31.087162	2
CAACGCA	5420	0.0	30.964462	5
AACGCAG	5760	0.0	29.132746	6
GTACATG	6620	0.0	25.697868	1
TACATGG	6695	0.0	24.81244	2
ACGCAGA	6805	0.0	24.309189	7
CGCAGAG	7010	0.0	23.191427	8
ACATGGG	7175	0.0	22.491013	3
GTATCGC	110	0.0028653222	21.6076	6
CATGGGG	5830	0.0	20.352766	4
GCAGAGT	7850	0.0	19.135365	9
AGAGTAC	6660	0.0	19.021395	10-11
ATGGGGG	3770	0.0	18.159723	5
CAGAGTA	7170	0.0	17.27062	10-11
GTGGTAT	1160	0.0	16.795692	1
GTATTAG	485	1.8189894E-12	16.656328	1
>>END_MODULE