FastQCFastQC Report
Mon 15 Aug 2016
SRR2921401_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921401_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1538153
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15150.060.158291
GTATCAA27250.039.542691
TCAACGC34050.030.67194
CAACGCA34350.030.4020465
ATCAACG35400.029.7684753
AACGCAG36900.028.6804896
TATCAAC36650.028.494142
GTACATG47650.027.9930521
TACATGG47750.027.6361982
ACATGGG51650.024.6303943
CATGGGG42050.023.7076444
ACGCAGA43550.023.6487317
CGCAGAG45200.021.842568
ATGGGGG26100.018.9147115
GCAGAGT49550.018.2977059
AGAGTAC41600.018.14306610-11
GTATATG4201.8189894E-1218.0832961
CAGAGTA45900.017.99465210-11
GAGTACA34350.017.8266541
GTCTTAG4751.8189894E-1216.988781
Produced by FastQC (version 0.11.5)