FastQCFastQC Report
Mon 15 Aug 2016
SRR2921401_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921401_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1538153
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13150.057.812021
GTATCAA26150.046.1515121
TCAACGC39050.030.5107444
TATCAAC39350.030.3987662
ATCAACG40100.029.948583
CAACGCA40800.029.1217085
AACGCAG44200.026.7723066
ACGCAGA53750.021.661897
GTACATG44300.021.451131
GTATAGG3351.8189894E-1221.2750381
CGCAGAG54900.020.9484428
AGAGTAC48950.020.24240310-11
TACATGG46600.019.863182
ATAATAC5500.018.9871653
TAAGTAC11150.018.7317313
CAGAGTA53500.018.6540979
GTAAGGT6950.018.4407714
GGACCGT1559.594564E-418.3962066
TAAGGTG8100.018.1892035
GCAGAGT59200.018.142439
Produced by FastQC (version 0.11.5)