##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921401_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1538153
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.99386276917836	31.0	31.0	34.0	26.0	34.0
2	30.172250744886888	31.0	31.0	34.0	26.0	34.0
3	30.21766040179358	33.0	31.0	34.0	26.0	34.0
4	33.703461229149504	37.0	35.0	37.0	30.0	37.0
5	33.335879460625826	37.0	35.0	37.0	27.0	37.0
6	33.266574261468136	37.0	35.0	37.0	27.0	37.0
7	33.07698909016203	37.0	35.0	37.0	26.0	37.0
8	33.0091128775876	37.0	35.0	37.0	26.0	37.0
9	34.48285313619646	39.0	35.0	39.0	26.0	39.0
10-11	34.28523820452192	39.0	35.0	39.0	25.0	39.0
12-13	34.18395374192295	39.0	35.0	39.0	25.0	39.0
14-15	35.24082097164586	40.0	36.0	41.0	24.0	41.0
16-17	35.18582481716709	40.0	36.0	41.0	24.0	41.0
18-19	35.13049026982361	40.0	36.0	41.0	22.5	41.0
20-21	35.07566509963573	40.0	36.0	41.0	22.0	41.0
22-23	34.940439605162815	40.0	35.5	41.0	19.5	41.0
24-25	34.84895650822773	39.5	35.0	41.0	19.0	41.0
26-27	34.67260084009848	39.0	35.0	41.0	18.0	41.0
28-29	34.4955947165204	39.0	35.0	41.0	17.0	41.0
30-31	34.314371847273975	39.0	34.0	41.0	16.0	41.0
32-33	34.24783847900696	39.0	34.5	41.0	16.0	41.0
34-35	34.36752650744107	39.0	35.0	41.0	16.0	41.0
36-37	34.31385044270628	39.0	35.0	41.0	15.0	41.0
38-39	34.215381044668504	39.0	35.0	41.0	11.0	41.0
40-41	34.0662671398749	39.0	34.0	41.0	9.0	41.0
42-43	33.9266158828153	39.0	34.0	41.0	8.5	41.0
44-45	33.750694501782334	39.0	34.0	41.0	4.5	41.0
46-47	33.55957794835754	39.0	33.5	41.0	2.0	41.0
48-49	33.3764631996947	39.0	33.0	40.5	2.0	41.0
50-51	33.20684808338312	38.5	33.0	40.0	2.0	41.0
52-53	33.01696742781765	38.0	33.0	40.0	2.0	41.0
54-55	32.79469337575651	38.0	33.0	40.0	2.0	41.0
56-57	32.51924418442118	38.0	32.5	40.0	2.0	41.0
58-59	32.277894331708225	37.0	32.0	40.0	2.0	41.0
60-61	31.99929298320778	37.0	32.0	40.0	2.0	41.0
62-63	31.715440856663804	36.0	31.5	39.5	2.0	41.0
64-65	31.400441958634804	36.0	31.0	39.0	2.0	41.0
66-67	31.076948782078247	35.0	31.0	39.0	2.0	40.5
68-69	30.724233219972266	35.0	31.0	38.5	2.0	40.0
70-71	30.35673564333327	35.0	30.5	37.5	2.0	40.0
72-73	29.983583232617303	35.0	30.0	37.0	2.0	39.0
74-75	29.58908574114539	35.0	30.0	36.5	2.0	39.0
76-77	27.02840712204833	32.0	26.0	34.5	2.0	36.0
78-79	28.589205365135978	34.0	29.0	35.0	2.0	37.0
80-81	28.800581931706404	34.0	29.0	35.0	2.0	37.0
82-83	28.658290495158806	34.0	29.0	35.0	2.0	36.5
84-85	28.432800898220137	34.0	29.0	35.0	2.0	36.0
86-87	28.186744751659944	34.0	29.0	35.0	2.0	36.0
88-89	27.903507323393708	34.0	29.0	35.0	2.0	35.0
90-91	27.70006364776456	34.0	29.0	35.0	2.0	35.0
92-93	27.547955242423868	34.0	28.5	35.0	2.0	35.0
94-95	27.42544272253801	34.0	28.0	35.0	2.0	35.0
96-97	27.20656950251373	34.0	27.0	35.0	2.0	35.0
98-99	26.896321432263242	34.0	27.0	35.0	2.0	35.0
100-101	25.790854355841063	32.5	23.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	75875.0
3	19179.0
4	9620.0
5	6396.0
6	5549.0
7	5278.0
8	5250.0
9	5602.0
10	5952.0
11	6223.0
12	6691.0
13	7013.0
14	7465.0
15	8161.0
16	8756.0
17	9420.0
18	8531.0
19	7181.0
20	6858.0
21	6779.0
22	7136.0
23	8136.0
24	9287.0
25	11412.0
26	13388.0
27	16382.0
28	19272.0
29	23071.0
30	28117.0
31	34184.0
32	43557.0
33	57308.0
34	81635.0
35	123352.0
36	211196.0
37	346950.0
38	262196.0
39	19794.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.02047364002577	23.76380473900339	15.67951529665953	26.53620632431131
2	13.830259617241241	24.21624953109224	34.059834255328965	27.893656596337546
3	15.788098087952	26.89207191521427	28.951120513832425	28.368709483001307
4	13.03585163147256	19.797358930465013	32.955310128347584	34.211479309714846
5	14.386002330517291	37.84476569977943	33.64918431894794	14.120047650755337
6	33.68191384026063	36.33069226983447	16.08420023365838	13.903193656246525
7	28.491741057932547	32.41313759182291	20.80946539058909	18.285655959655458
8	27.109915729497015	35.088307841538686	19.24880797741691	18.552968451547386
9	26.510821745301023	15.383255111812675	19.151670867592497	38.95425227529381
10-11	25.838743004511205	26.00282226741667	27.237515467821076	20.92091926025105
12-13	26.212365504014564	23.355166921301564	27.309722718850566	23.122744855833307
14-15	23.694609528750675	24.92807698834213	24.500244782046458	26.877068700860736
16-17	23.68832859171265	27.91726695411117	25.37964388434953	23.01476056982665
18-19	23.788862013177514	27.129529912710105	26.813826085288795	22.26778198882359
20-21	24.359415699349835	26.043195495357562	26.806029204630377	22.791359600662226
22-23	24.18754725641371	25.8801964176469	26.484298355623082	23.447957970316306
24-25	24.066627571625844	26.244919161684432	26.320660385454563	23.36779288123516
26-27	23.781824375962827	26.46401312780397	26.431960793428555	23.322201702804644
28-29	23.912632172317004	26.601818277571134	26.343569028034867	23.141980522076995
30-31	24.230092753642037	26.235105936818286	26.257831066780675	23.276970242759006
32-33	24.064716333673072	26.324683790193713	25.985431835065352	23.625168041067855
34-35	24.321872949884366	26.40698481102038	26.103608333555478	23.167533905539774
36-37	24.260381765916854	26.348876224976703	26.18179146716996	23.208950541936485
38-39	24.236899103044994	26.261801119982884	26.14783293030549	23.353466846666628
40-41	24.272000572140033	26.231352623554617	26.16396363009847	23.332683174206885
42-43	24.256689783818384	26.21870561505245	25.90317323624845	23.621431364880717
44-45	24.22804935256696	26.187659517611916	25.87134692274658	23.712944207074543
46-47	24.37002600780234	26.312028608582576	25.702438231469444	23.615507152145643
48-49	24.066387748221757	26.37265034400989	25.87390671241111	23.68705519535724
50-51	24.2345237058064	26.362168755225735	25.759592266111508	23.64371527285636
52-53	24.503055506885172	26.1790901841821	25.736863303328178	23.580991005604552
54-55	23.999310855968897	26.18025667364479	25.955959795597277	23.86447267478903
56-57	24.238617830280653	26.202826976196537	25.737147322837746	23.821407870685068
58-59	24.21970825676084	26.130040984232757	25.85937014903151	23.7908806099749
60-61	24.13673046446422	26.222211386078264	26.03697834125726	23.60407980820025
62-63	24.17927217859724	26.205940041686215	25.977644459052936	23.63714332066361
64-65	23.98886933630914	25.967852697540668	25.98066375589016	24.062614210260033
66-67	24.023970904550474	26.270511592819783	25.727687012562612	23.977830490067127
68-69	24.07299208842702	26.134852116044204	25.738151742363574	24.054004053165198
70-71	23.989829849307736	25.981457262019582	25.88127106722848	24.147441821444204
72-73	23.957434813157118	26.16053990380598	25.673489811451788	24.20853547158511
74-75	23.961213117493728	26.16683990754562	25.61171045482965	24.260236520131002
76-77	23.87616845259421	25.835879230042668	25.978210598312867	24.30974171905026
78-79	23.762262570232117	25.94071036722991	25.923636108187708	24.37339095435026
80-81	23.94399588671824	25.98652256304804	25.76202869866081	24.307452851572904
82-83	23.826694965400378	26.025178850337934	25.855366940680604	24.29275924358109
84-85	23.744171974035595	26.00059633724074	25.85281265353489	24.402419035188775
86-87	23.64879041479068	26.136272332574208	25.68632725827244	24.528609994362675
88-89	23.498102897682884	25.923350941448835	25.833008081662385	24.745538079205893
90-91	23.450886267137275	25.790906598985103	25.938816065875447	24.819391068002176
92-93	23.486539192575933	25.73823965827119	25.94795048236091	24.82727066679197
94-95	23.32364169726727	25.749725634810833	26.003285880725258	24.92334678719664
96-97	23.0796612996085	25.673553392579134	26.092101357850872	25.15468394996149
98-99	23.04685938154705	25.611767945079283	25.931438821255092	25.409933852118577
100-101	22.96139878216389	25.532526990426085	26.030044118278074	25.476030109131955
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	78.0
1	64.0
2	53.5
3	62.5
4	51.5
5	59.0
6	115.5
7	279.5
8	434.5
9	449.0
10	378.0
11	293.5
12	283.0
13	319.0
14	343.5
15	367.0
16	426.0
17	473.0
18	513.5
19	582.0
20	650.5
21	743.5
22	852.0
23	1010.0
24	1245.0
25	1518.0
26	1956.5
27	2613.5
28	3574.5
29	5005.5
30	6995.0
31	9805.5
32	13767.0
33	17147.0
34	19904.5
35	23570.5
36	28104.0
37	32584.5
38	36626.5
39	40517.5
40	43758.5
41	46624.5
42	49964.0
43	53344.0
44	56585.0
45	60113.0
46	62371.0
47	64195.0
48	66460.0
49	67466.0
50	67045.5
51	65878.0
52	64375.0
53	62831.0
54	61192.5
55	58138.0
56	53782.0
57	48142.0
58	42591.0
59	37801.0
60	31907.5
61	26453.5
62	21765.5
63	16926.0
64	12596.0
65	9382.5
66	7350.5
67	5893.0
68	4647.0
69	3632.5
70	2859.0
71	2183.0
72	1570.0
73	1206.5
74	975.0
75	762.5
76	556.5
77	380.0
78	235.5
79	152.5
80	106.0
81	62.0
82	41.5
83	25.0
84	12.5
85	8.5
86	6.5
87	4.5
88	3.5
89	3.0
90	3.0
91	2.5
92	0.5
93	1.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10031511819695439
2	2.6005215345937627E-4
3	0.00416083445535002
4	0.00416083445535002
5	0.01930887239435869
6	6.501303836484407E-4
7	1.3002607672968813E-4
8	7.151434220132848E-4
9	0.0
10-11	0.07092922485604489
12-13	1.950391150945322E-4
14-15	0.0032506519182422034
16-17	6.501303836484407E-5
18-19	1.6253259591211017E-4
20-21	4.225847493714864E-4
22-23	1.3002607672968813E-4
24-25	0.0013652738056617254
26-27	0.002828067168870717
28-29	0.0067613559899437825
30-31	0.013522711979887565
32-33	0.013685244575799676
34-35	0.00845169498742973
36-37	7.476499411957067E-4
38-39	2.925586726417983E-4
40-41	0.004420886608809397
42-43	0.007639032007869179
44-45	0.007639032007869179
46-47	0.009946994869821143
48-49	0.012254957731773106
50-51	0.00591618649120081
52-53	0.001852871593398056
54-55	8.451694987429728E-4
56-57	0.005103523511640259
58-59	0.007964097199693399
60-61	0.005883679972018389
62-63	0.015245557496555933
64-65	0.027240463074869666
66-67	0.029613438975186472
68-69	0.022266965639959096
70-71	0.03354672779625954
72-73	0.021031717911027058
74-75	0.020381587527378616
76-77	0.19523415420962675
78-79	0.04882479181199789
80-81	0.04515155514438421
82-83	0.01787858555033212
84-85	0.028118139092795057
86-87	0.06998653579975464
88-89	0.22390490412852299
90-91	0.2669760420452322
92-93	0.036374794965130255
94-95	0.003705743186796112
96-97	0.0063387712405722964
98-99	5.526108261011746E-4
100-101	9.751955754726609E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1538153.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.08019778867617
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.25790729801514	59.89068716002859
2	9.322519789505732	13.252931010538305
3	2.77652455815379	5.920677442760645
4	1.2368671312216917	3.5166704130220134
5	0.6631804468781869	2.3569498666842095
6	0.44347454881086773	1.8913355186232241
7	0.2882486487671636	1.4342139676682049
8	0.19520864105654798	1.11003750530865
9	0.14455556245333542	0.9247534173572772
>10	0.6438868687207994	7.435803546859364
>50	0.01936796921848535	0.9316498426647131
>100	0.007616271104734883	0.9583753954821722
>500	4.5876149531269594E-4	0.220305443463371
>1k	1.8350459812507838E-4	0.15560946953925495
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.501303836484407E-5
2	0.0	0.0	0.0	0.0	6.501303836484407E-5
3	0.0	0.0	0.0	0.0	6.501303836484407E-5
4	0.0	0.0	0.0	0.0	6.501303836484407E-5
5	0.0	0.0	0.0	0.0	6.501303836484407E-5
6	0.0	0.0	0.0	0.0	6.501303836484407E-5
7	0.0	0.0	0.0	0.0	1.3002607672968813E-4
8	0.0	0.0	0.0	0.0	1.3002607672968813E-4
9	0.0	0.0	0.0	0.0	1.3002607672968813E-4
10-11	0.0	0.0	0.0	0.0	1.3002607672968813E-4
12-13	0.0	0.0	0.0	0.0	1.6253259591211017E-4
14-15	0.0	0.0	0.0	0.0	2.2754563427695423E-4
16-17	0.0	0.0	0.0	6.501303836484407E-5	3.2506519182422033E-4
18-19	0.0	0.0	0.0	1.3002607672968813E-4	3.900782301890644E-4
20-21	0.0	0.0	0.0	1.3002607672968813E-4	4.8759778773633047E-4
22-23	0.0	0.0	0.0	1.950391150945322E-4	5.526108261011746E-4
24-25	0.0	0.0	0.0	3.2506519182422033E-4	6.501303836484407E-4
26-27	0.0	0.0	0.0	5.526108261011746E-4	6.501303836484407E-4
28-29	0.0	0.0	0.0	0.0014302868440265696	6.501303836484407E-4
30-31	0.0	0.0	0.0	0.005331069145917213	6.826369028308627E-4
32-33	0.0	0.0	0.0	0.014822972747184448	7.151434220132848E-4
34-35	0.0	0.0	0.0	0.027923099977700528	7.151434220132848E-4
36-37	0.0	0.0	0.0	0.05145781986577408	8.451694987429729E-4
38-39	0.0	0.0	0.0	0.08838522565700552	8.451694987429729E-4
40-41	0.0	0.0	0.0	0.12963599849949908	9.101825371078169E-4
42-43	0.0	0.0	0.0	0.1760228013728153	9.101825371078169E-4
44-45	0.0	0.0	0.0	0.24032069631564612	9.101825371078169E-4
46-47	0.0	0.0	0.0	0.3188239401411953	9.42689056290239E-4
48-49	0.0	0.0	0.0	0.3982048599846699	0.001040208613837505
50-51	0.0	0.0	0.0	0.4804463535161977	0.001040208613837505
52-53	0.0	0.0	0.0	0.5700343203829528	0.0011052216522023492
54-55	0.0	6.501303836484407E-5	0.0	0.6781835097028709	0.0011702346905671933
56-57	0.0	1.3002607672968813E-4	0.0	0.8089897428929371	0.0011702346905671933
58-59	0.0	1.3002607672968813E-4	0.0	0.9439893170575359	0.0011702346905671933
60-61	0.0	1.3002607672968813E-4	0.0	1.0825646083322011	0.0011702346905671933
62-63	0.0	1.3002607672968813E-4	0.0	1.2326472073974437	0.0012352477289320374
64-65	0.0	1.3002607672968813E-4	0.0	1.4035989917778011	0.0013002607672968813
66-67	6.501303836484407E-5	1.3002607672968813E-4	0.0	1.6164191728651183	0.0013327672864793033
68-69	6.501303836484407E-5	1.3002607672968813E-4	0.0	1.8465653286766661	0.0013652738056617254
70-71	6.501303836484407E-5	1.3002607672968813E-4	0.0	2.079929629887274	0.0014302868440265696
72-73	6.501303836484407E-5	1.3002607672968813E-4	0.0	2.333870557740355	0.0015603129207562576
74-75	6.501303836484407E-5	1.3002607672968813E-4	0.0	2.61108615332805	0.0015603129207562576
76-77	6.501303836484407E-5	1.3002607672968813E-4	0.0	2.9428801946230316	0.0016253259591211017
78-79	6.501303836484407E-5	1.3002607672968813E-4	0.0	3.3248968080548553	0.0016253259591211017
80-81	6.501303836484407E-5	1.3002607672968813E-4	0.0	3.708441227888253	0.0016253259591211017
82-83	6.501303836484407E-5	1.3002607672968813E-4	0.0	4.099299614537696	0.0016903389974859458
84-85	6.501303836484407E-5	1.3002607672968813E-4	0.0	4.526240237479627	0.0016903389974859458
86-87	6.501303836484407E-5	1.3002607672968813E-4	0.0	4.994301607187321	0.0016903389974859458
88-89	6.501303836484407E-5	1.3002607672968813E-4	0.0	5.525458130628097	0.0016903389974859458
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1315	0.0	57.81202	1
GTATCAA	2615	0.0	46.151512	1
TCAACGC	3905	0.0	30.510744	4
TATCAAC	3935	0.0	30.398766	2
ATCAACG	4010	0.0	29.94858	3
CAACGCA	4080	0.0	29.121708	5
AACGCAG	4420	0.0	26.772306	6
ACGCAGA	5375	0.0	21.66189	7
GTACATG	4430	0.0	21.45113	1
GTATAGG	335	1.8189894E-12	21.275038	1
CGCAGAG	5490	0.0	20.948442	8
AGAGTAC	4895	0.0	20.242403	10-11
TACATGG	4660	0.0	19.86318	2
ATAATAC	550	0.0	18.987165	3
TAAGTAC	1115	0.0	18.731731	3
CAGAGTA	5350	0.0	18.654097	9
GTAAGGT	695	0.0	18.440771	4
GGACCGT	155	9.594564E-4	18.396206	6
TAAGGTG	810	0.0	18.189203	5
GCAGAGT	5920	0.0	18.14243	9
>>END_MODULE