FastQCFastQC Report
Mon 15 Aug 2016
SRR2921400_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921400_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175728
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16950.055.1512221
GTATCAA29850.038.947571
TCAACGC37200.030.3628524
CAACGCA37950.029.6336565
TATCAAC37850.029.5906642
ATCAACG38250.029.2791983
AACGCAG40050.027.9542796
GTACATG50200.027.3182431
TAGTACG1301.0095631E-525.5542564
TACATGG53650.024.7683372
ACATGGG55550.023.577823
ACGCAGA48450.022.818177
CGCAGAG48800.022.0757068
CATGGGG48250.021.7371964
TAAGGCG5550.018.8095465
GAGTACA38900.018.297861
GCAGAGT57450.017.7601669
AGAGTAC46700.017.63137410-11
CGTATAC1350.00945489517.575793
CAGAGTA53400.017.01888510-11
Produced by FastQC (version 0.11.5)