##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921400_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2175728
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.484356040828633	31.0	31.0	34.0	26.0	34.0
2	30.556543373068692	33.0	31.0	34.0	26.0	34.0
3	30.598220457704272	33.0	31.0	34.0	26.0	34.0
4	34.052427968937295	37.0	35.0	37.0	30.0	37.0
5	34.04191378701749	37.0	35.0	37.0	30.0	37.0
6	34.0883189442798	37.0	35.0	37.0	30.0	37.0
7	34.00565144172433	37.0	35.0	37.0	30.0	37.0
8	33.97102624960473	37.0	35.0	37.0	30.0	37.0
9	35.54216427788768	39.0	37.0	39.0	30.0	39.0
10-11	35.442902789319255	39.0	37.0	39.0	30.0	39.0
12-13	35.339755245140935	39.0	37.0	39.0	29.0	39.0
14-15	36.50193567394454	40.0	37.0	41.0	29.0	41.0
16-17	36.452381685578345	40.0	37.0	41.0	29.0	41.0
18-19	36.40535236941382	40.0	37.0	41.0	28.5	41.0
20-21	36.34394143017877	40.0	37.0	41.0	28.0	41.0
22-23	36.227411238904864	40.0	37.0	41.0	27.0	41.0
24-25	36.1311099089592	40.0	36.5	41.0	27.0	41.0
26-27	35.9895168421788	40.0	36.0	41.0	27.0	41.0
28-29	35.85691226109146	40.0	36.0	41.0	27.0	41.0
30-31	35.71370754984079	40.0	36.0	41.0	26.5	41.0
32-33	35.54288794371355	40.0	35.5	41.0	25.5	41.0
34-35	35.3857605362435	39.5	35.0	41.0	25.0	41.0
36-37	35.21179554613444	39.0	35.0	41.0	25.0	41.0
38-39	35.44023862357795	39.5	35.5	41.0	25.5	41.0
40-41	35.56345600185317	40.0	36.0	41.0	25.0	41.0
42-43	35.398434684850315	40.0	35.0	41.0	24.5	41.0
44-45	35.276925700271356	40.0	35.0	41.0	24.0	41.0
46-47	35.12536976129369	40.0	35.0	41.0	23.5	41.0
48-49	34.98421401939948	40.0	35.0	41.0	23.0	41.0
50-51	33.26399301750954	37.0	32.5	39.5	18.5	40.0
52-53	33.4223558735283	37.5	33.5	39.5	19.5	40.0
54-55	34.28794040431524	39.0	34.0	40.5	20.5	41.0
56-57	34.2988588187494	39.0	34.0	41.0	20.0	41.0
58-59	34.12105074715222	38.5	34.0	41.0	20.0	41.0
60-61	33.877349328592544	38.0	34.0	40.5	18.5	41.0
62-63	33.598172427803476	37.5	34.0	40.0	17.5	41.0
64-65	33.30347704308627	37.0	34.0	40.0	15.0	41.0
66-67	32.961701554606094	36.5	33.0	39.5	13.0	41.0
68-69	32.59528442893597	36.0	33.0	39.0	9.5	41.0
70-71	32.21269409595317	35.0	33.0	39.0	7.5	40.5
72-73	31.854630266283287	35.0	32.5	37.5	4.0	40.0
74-75	31.450927000066187	35.0	32.0	37.0	2.0	39.0
76-77	31.074208954428126	35.0	32.0	36.5	2.0	39.0
78-79	30.731160788480913	35.0	32.0	36.0	2.0	38.5
80-81	30.383936319245787	35.0	31.5	36.0	2.0	37.0
82-83	30.09556617371289	35.0	31.0	35.0	2.0	37.0
84-85	29.865728620489328	35.0	31.0	35.0	2.0	36.5
86-87	29.647234167138542	35.0	31.0	35.0	2.0	36.0
88-89	29.45322462182773	35.0	31.0	35.0	2.0	36.0
90-91	29.20905761198091	35.0	31.0	35.0	2.0	35.5
92-93	29.028854250163622	34.5	30.5	35.0	2.0	35.0
94-95	28.837728796981978	34.0	30.0	35.0	2.0	35.0
96-97	28.600209676944914	34.0	30.0	35.0	2.0	35.0
98-99	28.381362008486356	34.0	30.0	35.0	2.0	35.0
100-101	27.343632338233455	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	73068.0
3	12464.0
4	8550.0
5	6536.0
6	5842.0
7	5992.0
8	6383.0
9	6856.0
10	7308.0
11	7955.0
12	7794.0
13	8049.0
14	8622.0
15	9421.0
16	9662.0
17	10153.0
18	9469.0
19	8659.0
20	8494.0
21	9059.0
22	10022.0
23	11273.0
24	13526.0
25	15881.0
26	18665.0
27	22002.0
28	25858.0
29	31057.0
30	37264.0
31	45078.0
32	56751.0
33	73473.0
34	101957.0
35	151341.0
36	256175.0
37	470761.0
38	513410.0
39	90888.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.43449278641213	18.926988576531535	13.656567551684272	24.981951085372057
2	16.28043550742133	19.750146587107235	38.61198680141184	25.357431104059604
3	19.509441684121462	23.058907923749175	29.156168881183998	28.275481510945365
4	13.898529563927756	15.752681620123935	35.626907025672274	34.721881790276036
5	14.98872018989443	36.17255304453709	34.003132412003225	14.835594353565257
6	34.08835880450597	34.41479128502047	16.85294448900974	14.64390542146382
7	29.338380061073742	31.104673478891037	20.76994469530473	18.787001764730487
8	26.738799338559087	34.14649619745032	19.917103819283383	19.197600644707208
9	26.467280972100653	15.809599641052422	19.45355196281068	38.26956742403625
10-11	25.757195097479666	25.061849255383926	27.657920462880448	21.52303518425596
12-13	26.227651120012833	22.542801303291007	27.353134946340617	23.87641263035554
14-15	23.524836509342258	24.26439550070767	24.56930523252873	27.641462757421344
16-17	23.76403750506881	27.136096100072365	25.568902276988375	23.530964117870447
18-19	23.701288252909855	26.530691055602396	26.78770261411166	22.980318077376086
20-21	24.236188453320555	25.66838035931227	26.767419381162718	23.32801180620446
22-23	24.11530051755098	25.522528757302847	26.42273637548772	23.939434349658452
24-25	23.917949566848755	25.808158141588038	26.399027938715076	23.874864352848128
26-27	23.73938114677408	25.939978565743903	26.488869313106118	23.831770974375903
28-29	23.852316467864036	26.082818110646343	26.429510226992196	23.635355194497425
30-31	24.051273258491836	25.80273342152997	26.257054888156063	23.888938431822133
32-33	23.82184934362427	25.848411427772927	26.164613582029382	24.16512564657342
34-35	24.113039641097302	25.86793042582923	26.15365521806281	23.865374715010663
36-37	24.10628906376576	25.800855315062194	26.223089278134587	23.869766343037462
38-39	23.937274470386775	25.866834653023673	26.29968603680822	23.896204839781326
40-41	24.055731659587906	25.732525999826716	26.365098629621315	23.846643710964063
42-43	24.03931304206232	25.668312732748376	26.147231133519288	24.14514309167001
44-45	23.99839925323045	25.77722679832456	26.163851352518197	24.060522595926802
46-47	24.099581576662242	25.811269452333107	26.025927003077097	24.063221967927557
48-49	23.848771581683135	25.923786186110952	26.05330909738995	24.174133134815968
50-51	23.885105979289527	25.92854491582169	26.18102458159526	24.005324523293524
52-53	24.07626333777139	25.798262940787183	26.132544829971476	23.99292889146995
54-55	23.82403728872024	25.692065485528676	26.218481744678098	24.265415481072985
56-57	23.85016250488505	25.891416384425785	26.11134434477703	24.147076765912136
58-59	23.816558440810766	25.740075560822405	26.249072530744076	24.194293467622746
60-61	23.77944726402463	25.68364264273808	26.4105231983205	24.126386894916788
62-63	23.828332889260523	25.735028777990692	26.35860372454651	24.078034608202277
64-65	23.728395522310723	25.633571293193125	26.247734798537834	24.39029838595832
66-67	23.698961564030053	25.72980309161342	26.178064240402637	24.393171103953897
68-69	23.775765658805987	25.632242453939813	26.252557108369817	24.339434778884385
70-71	23.651401746513763	25.532276891269962	26.322329176173188	24.493992186043084
72-73	23.76185670667618	25.5390877315133	26.23654309697167	24.462512464838852
74-75	23.757081496643593	25.692665635933075	26.198147212241707	24.352105655181624
76-77	23.753863722965452	25.50000748281543	26.280384547640782	24.46574424657834
78-79	23.657820288541348	25.520944166392606	26.326311871907613	24.494923673158432
80-81	23.77050822793571	25.583309706055164	26.249633498881668	24.396548567127457
82-83	23.711622996422268	25.60039964457612	26.325987630180443	24.36198972882116
84-85	23.724919035715487	25.57955503844048	26.325402955939126	24.370122969904916
86-87	23.697341404008096	25.568917613423032	26.299802317915727	24.433938664653144
88-89	23.58735817147448	25.43600653623978	26.364338703264938	24.612296589020794
90-91	23.582649179729735	25.41135594399377	26.43075671434896	24.575238161927537
92-93	23.644745907637915	25.338886947540047	26.59303413321257	24.423333011609472
94-95	23.62125126103136	25.19599980924705	26.56962839804612	24.613120531675474
96-97	23.66010912646303	25.052872241313057	26.649291396776338	24.637727235447578
98-99	23.500537644811658	25.11883931376804	26.56875265482282	24.811870386597487
100-101	23.714916292110242	24.931174536767195	26.72455300885343	24.629356162269133
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	47.0
1	37.0
2	47.0
3	77.0
4	81.0
5	95.5
6	141.0
7	273.0
8	413.5
9	479.5
10	465.5
11	410.5
12	436.5
13	481.5
14	539.5
15	608.5
16	703.0
17	821.5
18	906.5
19	988.5
20	1071.0
21	1178.5
22	1313.5
23	1493.5
24	1671.0
25	1934.5
26	2414.0
27	3099.0
28	4052.5
29	5406.5
30	7383.5
31	10482.0
32	14980.5
33	19398.0
34	23582.5
35	28774.5
36	35673.5
37	42661.5
38	49082.5
39	55252.0
40	60732.0
41	65753.5
42	71354.0
43	77125.0
44	82340.5
45	88107.5
46	91917.0
47	94225.0
48	97042.5
49	98334.5
50	97467.0
51	95855.0
52	94326.0
53	92397.5
54	89825.5
55	84843.5
56	77789.0
57	70140.0
58	63293.5
59	55962.5
60	46306.5
61	37791.5
62	30882.0
63	23595.5
64	17147.0
65	12636.5
66	9436.5
67	7378.0
68	5746.5
69	4399.5
70	3313.5
71	2398.5
72	1766.5
73	1328.0
74	961.0
75	734.0
76	565.0
77	387.0
78	261.0
79	193.0
80	150.0
81	112.5
82	78.5
83	61.0
84	49.0
85	39.0
86	38.5
87	33.5
88	26.5
89	24.5
90	25.0
91	22.5
92	16.0
93	10.0
94	10.5
95	13.0
96	9.0
97	4.5
98	2.5
99	3.5
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04973048101600936
2	0.057589919328151315
3	0.04885721009243802
4	0.055291837950332025
5	0.04807586242397947
6	0.01806291962965959
7	0.01493752895582536
8	0.02192369634439599
9	0.022061581227065148
10-11	0.022038600413286955
12-13	0.028450247457402766
14-15	0.045318164770596324
16-17	0.030426597442327348
18-19	0.024543509115109975
20-21	0.027599957347609626
22-23	0.04885721009243802
24-25	0.044490855474581384
26-27	0.03240294742725194
28-29	0.020912540538155503
30-31	0.013145025481126317
32-33	0.010157519689961245
34-35	0.009560018531728231
36-37	0.0017695226609208505
38-39	0.007101071457461595
40-41	0.004067604038740137
42-43	0.22036302331909136
44-45	0.19494624328041005
46-47	0.2005076002147327
48-49	0.19752009442356766
50-51	0.1960723031555415
52-53	0.20025481126317257
54-55	0.19981817580138694
56-57	0.20969992572600987
58-59	0.2066894391210666
60-61	0.21146944838693074
62-63	0.2085508850371002
64-65	0.20974588735356625
66-67	0.19935855952582307
68-69	0.19901384731915017
70-71	0.18260554628152048
72-73	0.18933892471853098
74-75	0.19007431075943315
76-77	0.18777622938161387
78-79	0.21464080068832134
80-81	0.2234424523653692
82-83	0.2205698506430951
84-85	0.21643330416302037
86-87	0.23307141333843204
88-89	0.23261179706286816
90-91	0.23169256451174045
92-93	0.23576016855048057
94-95	0.24734249869468977
96-97	0.24828471205959568
98-99	0.23670238191538648
100-101	0.24741144113602434
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2175728.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.60282441480516
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.40631340038456	54.218903976624134
2	10.807096774419321	14.395663378009147
3	3.2983253589488033	6.59033354234899
4	1.4850962754195478	3.9564642588339707
5	0.810216521658318	2.6981354364991565
6	0.5271912406141945	2.1067455378990303
7	0.34713011807939703	1.6183892412472867
8	0.24817177964945902	1.322315317176211
9	0.18964862274193175	1.1368020528891454
>10	0.8449812534528983	9.348169601369417
>50	0.026471281288769532	1.165704592005294
>100	0.00852679574497489	1.0191841386786533
>500	6.921479981904678E-4	0.314210863014729
>1k	1.3842959963806224E-4	0.10897806340473663
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.192325511277145E-5
2	0.0	0.0	0.0	4.5961627556385725E-5	9.192325511277145E-5
3	0.0	0.0	0.0	4.5961627556385725E-5	9.192325511277145E-5
4	0.0	0.0	0.0	4.5961627556385725E-5	9.192325511277145E-5
5	0.0	0.0	0.0	4.5961627556385725E-5	9.192325511277145E-5
6	0.0	0.0	0.0	4.5961627556385725E-5	1.838465102255429E-4
7	0.0	0.0	0.0	4.5961627556385725E-5	1.838465102255429E-4
8	0.0	0.0	0.0	4.5961627556385725E-5	1.838465102255429E-4
9	0.0	0.0	0.0	4.5961627556385725E-5	1.838465102255429E-4
10-11	0.0	0.0	0.0	4.5961627556385725E-5	1.838465102255429E-4
12-13	0.0	0.0	0.0	4.5961627556385725E-5	4.1365464800747153E-4
14-15	0.0	0.0	0.0	4.5961627556385725E-5	6.204819720112073E-4
16-17	0.0	0.0	0.0	4.5961627556385725E-5	7.583668546803645E-4
18-19	0.0	0.0	0.0	4.5961627556385725E-5	8.732709235713288E-4
20-21	0.0	0.0	0.0	9.192325511277145E-5	9.422133649059074E-4
22-23	0.0	0.0	0.0	2.987505791165072E-4	0.001034136620018679
24-25	0.0	0.0	0.0	4.825970893420501E-4	0.0011490406889096431
26-27	0.0	0.0	0.0	9.192325511277145E-4	0.0011950023164660289
28-29	0.0	2.2980813778192863E-5	0.0	0.003814815087180015	0.0011950023164660289
30-31	0.0	4.5961627556385725E-5	0.0	0.014960509769603555	0.0011950023164660289
32-33	0.0	4.5961627556385725E-5	0.0	0.04049219387717582	0.0011950023164660289
34-35	0.0	4.5961627556385725E-5	0.0	0.07687082208805512	0.0012179831302442218
36-37	0.0	4.5961627556385725E-5	0.0	0.1332887199135186	0.0012639447578006073
38-39	0.0	4.5961627556385725E-5	0.0	0.21466378150209953	0.0013788488266915718
40-41	0.0	4.5961627556385725E-5	0.0	0.30072692910143184	0.0014248104542479575
42-43	0.0	4.5961627556385725E-5	0.0	0.4009462579881309	0.0014248104542479575
44-45	0.0	4.5961627556385725E-5	0.0	0.5280071773677593	0.0015397145231389217
46-47	0.0	4.5961627556385725E-5	0.0	0.6749924622930807	0.001677599405808079
48-49	0.0	4.5961627556385725E-5	0.0	0.8144170594853768	0.0017465418471426576
50-51	0.0	4.5961627556385725E-5	0.0	0.9563006037519396	0.0017465418471426576
52-53	0.0	4.5961627556385725E-5	0.0	1.1059746438893097	0.0018844267298118147
54-55	0.0	6.894244133457859E-5	0.0	1.2924179860717884	0.0019303883573682005
56-57	0.0	9.192325511277145E-5	0.0	1.50731157571167	0.001976349984924586
58-59	0.0	9.192325511277145E-5	0.0	1.721952376399991	0.001976349984924586
60-61	0.0	9.192325511277145E-5	0.0	1.934157210827824	0.001976349984924586
62-63	0.0	9.192325511277145E-5	0.0	2.156289756807836	0.002160196495150129
64-65	0.0	9.192325511277145E-5	0.0	2.4134680437995923	0.002160196495150129
66-67	0.0	9.192325511277145E-5	0.0	2.7256623989763424	0.002160196495150129
68-69	0.0	9.192325511277145E-5	0.0	3.0513924534684485	0.002160196495150129
70-71	0.0	9.192325511277145E-5	0.0	3.376525006802321	0.0022521197502629008
72-73	0.0	9.192325511277145E-5	0.0	3.708528823455873	0.0025049087018230217
74-75	0.0	9.192325511277145E-5	0.0	4.080588198524816	0.002665774398270372
76-77	0.0	9.192325511277145E-5	0.0	4.512144900465499	0.0027117360258267576
78-79	0.0	9.192325511277145E-5	0.0	4.981022443981969	0.0027576976533831435
80-81	0.0	9.192325511277145E-5	0.0	5.450152776449998	0.0027576976533831435
82-83	0.0	9.192325511277145E-5	0.0	5.924407830390564	0.0029185633498304935
84-85	0.0	9.192325511277145E-5	0.0	6.446554900244884	0.0029415441636086864
86-87	0.0	9.192325511277145E-5	0.0	7.039276049212034	0.003033467418721458
88-89	0.0	9.192325511277145E-5	0.0	7.681681717567637	0.0031253906738342293
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1695	0.0	55.151222	1
GTATCAA	2985	0.0	38.94757	1
TCAACGC	3720	0.0	30.362852	4
CAACGCA	3795	0.0	29.633656	5
TATCAAC	3785	0.0	29.590664	2
ATCAACG	3825	0.0	29.279198	3
AACGCAG	4005	0.0	27.954279	6
GTACATG	5020	0.0	27.318243	1
TAGTACG	130	1.0095631E-5	25.554256	4
TACATGG	5365	0.0	24.768337	2
ACATGGG	5555	0.0	23.57782	3
ACGCAGA	4845	0.0	22.81817	7
CGCAGAG	4880	0.0	22.075706	8
CATGGGG	4825	0.0	21.737196	4
TAAGGCG	555	0.0	18.809546	5
GAGTACA	3890	0.0	18.29786	1
GCAGAGT	5745	0.0	17.760166	9
AGAGTAC	4670	0.0	17.631374	10-11
CGTATAC	135	0.009454895	17.57579	3
CAGAGTA	5340	0.0	17.018885	10-11
>>END_MODULE