FastQCFastQC Report
Mon 15 Aug 2016
SRR2921400_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921400_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175728
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17050.046.5516361
GTATCAA28800.039.4410361
TCAACGC35400.031.2303054
ATCAACG36250.030.3671173
TATCAAC37100.030.0550562
CAACGCA36700.029.7930265
AACGCAG39200.027.890396
GTACATG46950.024.9024681
TACATGG50500.022.831712
ACGCAGA48650.021.8865877
CGCAGAG49800.021.0948188
ACATGGG52700.021.0682663
TATACCG2251.6558661E-619.0157245
CATGGGG47300.017.8559284
GTATAGG4950.017.2826421
GCAGAGT59300.016.9137789
AGAGTAC48050.016.57044810-11
GTATTAA10950.016.493481
TCTATAC7000.016.2680973
GAGTACT26850.016.16815412-13
Produced by FastQC (version 0.11.5)