##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921400_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2175728
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.40765711522764	33.0	31.0	34.0	29.0	34.0
2	31.544530842090555	34.0	31.0	34.0	29.0	34.0
3	31.622483141275012	34.0	31.0	34.0	28.0	34.0
4	35.092562581352084	37.0	35.0	37.0	33.0	37.0
5	34.82373761793754	37.0	35.0	37.0	32.0	37.0
6	34.77933225109021	37.0	35.0	37.0	32.0	37.0
7	34.64477407102358	37.0	35.0	37.0	32.0	37.0
8	34.60369770486017	37.0	35.0	37.0	32.0	37.0
9	36.23161534897744	39.0	37.0	39.0	32.0	39.0
10-11	36.08273621518866	39.0	37.0	39.0	32.0	39.0
12-13	36.02155784178905	39.0	37.0	39.0	31.5	39.0
14-15	37.27801522065258	40.0	38.0	41.0	31.0	41.0
16-17	37.25980752189612	40.0	38.0	41.0	31.0	41.0
18-19	37.21640388872139	40.0	38.0	41.0	31.0	41.0
20-21	37.17948176426465	40.0	38.0	41.0	31.0	41.0
22-23	37.06682751704257	40.0	38.0	41.0	31.0	41.0
24-25	37.00407426847474	40.0	38.0	41.0	31.0	41.0
26-27	36.8740030922983	40.0	37.0	41.0	30.0	41.0
28-29	36.721499884176694	40.0	37.0	41.0	30.0	41.0
30-31	36.58544289543546	40.0	37.0	41.0	30.0	41.0
32-33	36.54103178338468	40.0	37.0	41.0	30.0	41.0
34-35	36.67193969099078	40.0	37.0	41.0	30.0	41.0
36-37	36.64914663046116	40.0	37.0	41.0	30.0	41.0
38-39	36.559536853871435	40.0	37.0	41.0	30.0	41.0
40-41	36.438737057205685	40.0	37.0	41.0	30.0	41.0
42-43	36.33363453519925	40.0	37.0	41.0	29.0	41.0
44-45	36.19852527521823	40.0	36.0	41.0	29.0	41.0
46-47	36.04786650721046	40.0	36.0	41.0	28.0	41.0
48-49	35.88823442084672	40.0	36.0	41.0	28.0	41.0
50-51	35.73170635300001	40.0	35.0	41.0	27.0	41.0
52-53	35.55854523175691	39.0	35.0	41.0	27.0	41.0
54-55	35.34829767323856	39.0	35.0	41.0	26.5	41.0
56-57	35.08151777244214	39.0	35.0	41.0	26.0	41.0
58-59	34.85544677459683	38.5	35.0	41.0	26.0	41.0
60-61	34.58072424494238	38.0	34.5	40.0	25.5	41.0
62-63	34.28755455645191	37.5	34.0	40.0	25.0	41.0
64-65	33.96680651257878	37.0	34.0	40.0	24.5	41.0
66-67	33.621012828809484	36.5	34.0	39.5	23.0	41.0
68-69	33.25206390688542	36.0	34.0	39.0	22.5	41.0
70-71	32.87024733790253	35.5	33.0	39.0	22.0	40.0
72-73	32.46879274431363	35.0	33.0	37.5	21.0	40.0
74-75	32.05877710816793	35.0	33.0	37.0	20.0	39.0
76-77	29.310670957031395	33.0	29.0	34.5	13.0	36.5
78-79	30.96352094563291	34.5	31.5	36.0	17.0	37.0
80-81	31.155382014663594	35.0	32.0	36.0	18.0	37.0
82-83	30.99747808549598	35.0	32.0	35.0	17.5	37.0
84-85	30.770750985417294	35.0	32.0	35.0	14.0	36.0
86-87	30.53155702367208	35.0	32.0	35.0	8.5	36.0
88-89	30.260473046263137	35.0	32.0	35.0	2.0	36.0
90-91	30.084375896251736	35.0	32.0	35.0	2.0	35.0
92-93	29.949757276644874	34.0	31.0	35.0	2.0	35.0
94-95	29.859473013170764	34.0	31.0	35.0	2.0	35.0
96-97	29.679014104704265	34.0	31.0	35.0	2.0	35.0
98-99	29.4209246284462	34.0	31.0	35.0	2.0	35.0
100-101	28.339535088944942	33.5	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	42795.0
3	14596.0
4	7621.0
5	5479.0
6	4604.0
7	4690.0
8	4840.0
9	5208.0
10	5785.0
11	6161.0
12	6571.0
13	7167.0
14	7805.0
15	8532.0
16	9268.0
17	9975.0
18	9323.0
19	8462.0
20	7902.0
21	7785.0
22	7946.0
23	9110.0
24	10434.0
25	12802.0
26	15372.0
27	18493.0
28	22116.0
29	26959.0
30	32834.0
31	40728.0
32	52415.0
33	69042.0
34	97744.0
35	148711.0
36	265742.0
37	524328.0
38	565631.0
39	70750.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.47493879820763	20.091174328667666	13.895223636274917	25.538663236849786
2	15.718881235231908	21.074209422991274	36.88685268010007	26.320056661676748
3	18.31079786009146	24.02676586350384	28.917860624644064	28.744575651760634
4	13.677254124732954	16.856479149277277	34.64293068389488	34.82333604209489
5	14.846393865960875	36.578364345116015	33.93499547845404	14.640246310469069
6	34.22058841779125	34.89421100988694	16.535053791907796	14.350146780414018
7	29.377104183662915	31.29393650392398	20.63284652242356	18.696112789989545
8	26.86672329253697	34.53369147357797	19.61815226843604	18.981432965449027
9	26.454088011001375	15.824956060684055	19.280626990138472	38.440328938176094
10-11	25.7656327102449	25.34671299858865	27.49838677952884	21.389267511637616
12-13	26.274427523369255	22.71737841507325	27.330398828526807	23.67779523303069
14-15	23.50573189736938	24.433641747968423	24.480226844368776	27.580399510293425
16-17	23.79377972001938	27.436938175005814	25.415172526637136	23.354109578337674
18-19	23.77568171654362	26.729966755847833	26.70716971523463	22.787181812373923
20-21	24.25143975989447	25.842253793014695	26.61731113061943	23.288995316471407
22-23	24.153786262117247	25.656483528908346	26.38780431866034	23.80192589031406
24-25	23.916234370759245	25.978844867339756	26.341925169009638	23.762995592891357
26-27	23.767105421911715	26.195882863265783	26.419676765123267	23.617334949699227
28-29	23.933615741302532	26.236132408153058	26.28039654785675	23.549855302687657
30-31	24.1170389159774	25.972370791480476	26.140433460100514	23.77015683244161
32-33	23.889573950448025	25.9828743548578	26.059894938499607	24.067656756194573
34-35	24.097373293019697	26.040127468319664	26.125144654478344	23.7373545841823
36-37	24.1449410250665	26.004354895235473	26.200681385982755	23.65002269371528
38-39	24.016637228176567	26.06460013521942	26.200187248100974	23.71857538850304
40-41	24.08252257941895	25.932749080310547	26.254430677351543	23.73029766291896
42-43	24.140781696117998	25.84429096520241	26.04355197575221	23.971375362927386
44-45	24.037233063339002	25.95052859302256	26.03025594341082	23.981982400227622
46-47	24.14675499257988	25.9620359149043	25.98033053038067	23.910878562135156
48-49	23.901976886336794	26.048298146627403	25.996539133100878	24.05318583393493
50-51	23.849578551880544	26.089725380746593	26.06554807560602	23.995147991766846
52-53	24.223654870187247	25.89592780042575	25.939706985112547	23.94071034427445
54-55	23.82175189788708	25.852308373959936	26.10158343785865	24.224356290294335
56-57	23.85125982554529	26.023049496249044	25.977130685670343	24.148559992535322
58-59	23.88127724797754	25.94822214882932	26.060954568949608	24.109546034243536
60-61	23.791800799938773	25.89991962920496	26.21692116652337	24.0913584043329
62-63	23.792033212607716	25.94500529462697	26.129392086722014	24.1335694060433
64-65	23.698221213253255	25.81494870542257	26.036840139084454	24.449989942239718
66-67	23.665434652317458	25.948902785354775	25.939591984619014	24.44607057770875
68-69	23.643943120641065	25.920082937571546	26.008606382147274	24.427367559640114
70-71	23.64000825818887	25.734558083438053	26.040193837533472	24.585239820839604
72-73	23.672222839038344	25.74396644642883	25.981605393838624	24.602205320694196
74-75	23.55884948398535	25.875300923302685	25.953087571353272	24.612762021358687
76-77	23.61844909129863	25.659704064448803	26.016826352685328	24.705020491567243
78-79	23.531213685529128	25.658458326596982	26.0274300238185	24.782897964055387
80-81	23.49391495655117	25.77601319284511	25.991762749527254	24.73830910107647
82-83	23.452559089958328	25.722077796702504	26.059964556368477	24.7653985569707
84-85	23.451599836873786	25.69251628618443	26.00042390746128	24.8554599694805
86-87	23.353961238612715	25.748119982556823	25.968298683113787	24.929620095716672
88-89	23.31493780073424	25.591480005889395	25.956456712126695	25.13712548124967
90-91	23.1401087025435	25.589985072132148	26.048380124610233	25.221526100714115
92-93	23.164800255318543	25.496053530438758	26.1740364679678	25.1651097462749
94-95	23.03911237992191	25.36302945074624	26.2259576657732	25.37190050355865
96-97	23.011084833566613	25.26542235215434	26.235063744113408	25.48842907016564
98-99	22.863180982295805	25.330296148502157	26.1299472031747	25.67657566602733
100-101	22.802397119632374	25.21732715691472	26.196347878803756	25.783927844649146
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	28.0
1	23.0
2	36.5
3	55.5
4	47.0
5	55.0
6	114.5
7	227.5
8	328.5
9	367.0
10	339.0
11	283.5
12	270.0
13	294.0
14	335.5
15	376.0
16	409.0
17	452.0
18	533.5
19	604.5
20	661.0
21	787.5
22	919.5
23	1075.5
24	1294.5
25	1643.0
26	2183.0
27	2913.0
28	3942.0
29	5377.5
30	7485.0
31	10512.0
32	15059.5
33	19508.5
34	23483.5
35	28633.0
36	35471.0
37	42381.5
38	48780.5
39	54969.0
40	60503.5
41	65937.0
42	71510.5
43	77446.0
44	82954.5
45	88450.5
46	92274.0
47	94779.5
48	97782.5
49	99219.0
50	98353.0
51	96721.0
52	95515.0
53	93846.0
54	91282.5
55	86688.5
56	79535.5
57	71024.0
58	63442.5
59	55654.5
60	45996.0
61	37478.0
62	30366.5
63	22942.5
64	16379.0
65	11901.0
66	8877.5
67	6862.5
68	5301.0
69	4024.0
70	3004.5
71	2184.5
72	1530.5
73	1109.0
74	851.5
75	626.0
76	426.0
77	268.0
78	168.0
79	97.0
80	58.0
81	35.0
82	21.0
83	13.0
84	8.0
85	8.5
86	10.5
87	6.5
88	2.5
89	2.0
90	1.0
91	1.0
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15654530345704978
2	3.217313928947001E-4
3	0.005561356934322673
4	0.0033092371840597722
5	0.02697947537559842
6	0.001332887199135186
7	1.3788488266915718E-4
8	0.002022311612480972
9	0.0
10-11	0.10481549164233764
12-13	2.7576976533831435E-4
14-15	0.006411647044115809
16-17	2.7576976533831435E-4
18-19	3.217313928947001E-4
20-21	8.273092960149431E-4
22-23	3.217313928947001E-4
24-25	0.0017695226609208505
26-27	0.003010486604943265
28-29	0.00698616738857063
30-31	0.016040608017178618
32-33	0.01542012604516741
34-35	0.013122044667348126
36-37	7.124052271239787E-4
38-39	2.2980813778192865E-4
40-41	0.005952030768551951
42-43	0.008709728421935094
44-45	0.008663766794378708
46-47	0.010318385386408595
48-49	0.012018965605994867
50-51	0.006158858092555687
52-53	0.0016775994058080789
54-55	8.732709235713288E-4
56-57	0.006848282505901473
58-59	0.010226462131295824
60-61	0.008571843539265938
62-63	0.018821286484339957
64-65	0.03633266658332292
66-67	0.038354978195803885
68-69	0.027485053278718665
70-71	0.042146812469205715
72-73	0.026634763168925525
74-75	0.025163991087121185
76-77	0.2446537434826412
78-79	0.07250446747019848
80-81	0.05588933910856504
82-83	0.020361001007478875
84-85	0.03332217997837965
86-87	0.08328246913217094
88-89	0.26319926020164286
90-91	0.32014571674400477
92-93	0.05467135597832082
94-95	0.005170683100093394
96-97	0.010709059220637874
98-99	0.001332887199135186
100-101	0.0015397145231389217
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2175728.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.0205172225917
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.95016133913597	53.44371520862005
2	11.008461716404023	14.535686726841865
3	3.421493560047085	6.776663235242254
4	1.519919733982912	4.013835478975034
5	0.8414691592471786	2.777711456017906
6	0.5319818229397494	2.1073029062099677
7	0.3645802204455326	1.6848842306058396
8	0.2552030170705538	1.3478908147011093
9	0.1989818533972511	1.1823196391276791
>10	0.8730265203137688	9.599316560546987
>50	0.0254760811259577	1.1217784924855847
>100	0.008405577682384608	0.9992675348841397
>500	6.994985063246372E-4	0.30622788986293
>1k	1.3989970126480393E-4	0.10339982587884639
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.2980813778192863E-4
2	0.0	0.0	0.0	0.0	2.2980813778192863E-4
3	0.0	0.0	0.0	0.0	2.2980813778192863E-4
4	0.0	0.0	0.0	0.0	2.2980813778192863E-4
5	0.0	0.0	0.0	0.0	2.2980813778192863E-4
6	0.0	0.0	0.0	0.0	2.2980813778192863E-4
7	0.0	0.0	0.0	0.0	2.2980813778192863E-4
8	0.0	0.0	0.0	0.0	2.2980813778192863E-4
9	0.0	0.0	0.0	0.0	2.7576976533831435E-4
10-11	0.0	0.0	0.0	0.0	3.217313928947001E-4
12-13	0.0	0.0	0.0	0.0	5.05577903120243E-4
14-15	0.0	0.0	0.0	0.0	5.745203444548216E-4
16-17	0.0	0.0	0.0	2.2980813778192863E-5	7.353860409021716E-4
18-19	0.0	0.0	0.0	4.5961627556385725E-5	7.813476684585573E-4
20-21	0.0	0.0	0.0	1.3788488266915718E-4	7.813476684585573E-4
22-23	0.0	0.0	0.0	2.987505791165072E-4	9.192325511277145E-4
24-25	0.0	0.0	0.0	4.825970893420501E-4	0.0010571174337968717
26-27	0.0	0.0	0.0	8.962517373495216E-4	0.0011260598751314504
28-29	0.0	0.0	0.0	0.003745872645845437	0.0011490406889096431
30-31	0.0	0.0	0.0	0.01500647139715994	0.0011720215026878359
32-33	0.0	0.0	0.0	0.04131950317319077	0.0011950023164660289
34-35	0.0	0.0	0.0	0.07843351742497223	0.0012869255715788003
36-37	0.0	0.0	0.0	0.135839590242898	0.001332887199135186
38-39	0.0	0.0	0.0	0.21783513380349012	0.001332887199135186
40-41	0.0	0.0	0.0	0.3047485715126156	0.001332887199135186
42-43	0.0	0.0	0.0	0.4050368428406492	0.0013558680129133788
44-45	0.0	0.0	0.0	0.5323275703580594	0.0014018296404697647
46-47	0.0	0.0	0.0	0.6797724715589448	0.0014937528955825362
48-49	0.0	0.0	0.0	0.8202541861850379	0.0015626953369171147
50-51	0.0	0.0	0.0	0.9618619606862623	0.0016086569644735004
52-53	0.0	0.0	0.0	1.1115589816374105	0.0018844267298118145
54-55	0.0	0.0	0.0	1.2983470360265623	0.001976349984924586
56-57	0.0	0.0	0.0	1.5131257215975524	0.001976349984924586
58-59	0.0	0.0	0.0	1.7283870042578853	0.001976349984924586
60-61	0.0	0.0	0.0	1.9409825125199474	0.001976349984924586
62-63	0.0	0.0	0.0	2.1632069817550725	0.001976349984924586
64-65	0.0	0.0	0.0	2.4199026716574865	0.001976349984924586
66-67	0.0	0.0	0.0	2.732005103579124	0.001976349984924586
68-69	0.0	0.0	0.0	3.0579190045814553	0.001976349984924586
70-71	0.0	0.0	0.0	3.3839937712802337	0.002160196495150129
72-73	0.0	0.0	0.0	3.716572108278241	0.002298081377819286
74-75	0.0	0.0	0.0	4.091366200186788	0.0023900046329320577
76-77	0.0	0.0	0.0	4.525887427104859	0.0025049087018230217
78-79	0.0	0.0	0.0	4.99867170896362	0.0025738511431576006
80-81	0.0	0.0	0.0	5.471731760587721	0.0025968319569357936
82-83	0.0	0.0	0.0	5.94899730113323	0.002619812770713986
84-85	0.0	0.0	0.0	6.47711938256988	0.002619812770713986
86-87	0.0	0.0	0.0	7.076321121022481	0.0027117360258267576
88-89	0.0	0.0	0.0	7.725230359677312	0.002803659280939529
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1705	0.0	46.551636	1
GTATCAA	2880	0.0	39.441036	1
TCAACGC	3540	0.0	31.230305	4
ATCAACG	3625	0.0	30.367117	3
TATCAAC	3710	0.0	30.055056	2
CAACGCA	3670	0.0	29.793026	5
AACGCAG	3920	0.0	27.89039	6
GTACATG	4695	0.0	24.902468	1
TACATGG	5050	0.0	22.83171	2
ACGCAGA	4865	0.0	21.886587	7
CGCAGAG	4980	0.0	21.094818	8
ACATGGG	5270	0.0	21.068266	3
TATACCG	225	1.6558661E-6	19.015724	5
CATGGGG	4730	0.0	17.855928	4
GTATAGG	495	0.0	17.282642	1
GCAGAGT	5930	0.0	16.913778	9
AGAGTAC	4805	0.0	16.570448	10-11
GTATTAA	1095	0.0	16.49348	1
TCTATAC	700	0.0	16.268097	3
GAGTACT	2685	0.0	16.168154	12-13
>>END_MODULE