FastQCFastQC Report
Mon 15 Aug 2016
SRR2921399_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921399_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3391515
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26300.043.8467561
GTATCAA45550.040.6315271
TCAACGC50350.035.1583374
CAACGCA50600.034.9815335
ATCAACG52700.033.499023
TATCAAC54600.032.162312
AACGCAG55250.032.1166466
GTACATG84000.026.1570421
ACGCAGA65450.025.8085277
TACATGG90250.024.0855452
CGCAGAG68900.023.9677758
ACATGGG92850.022.5912423
CATGGGG78800.019.8147534
GTATAGC9700.019.5692941
GAGTACA64700.019.143581
GCAGAGT84500.018.8129049
GTGTAGC17200.017.6578771
AGAGTAC65750.017.21340410-11
GTCTTAG10800.017.1367231
GTATATA13300.017.1268121
Produced by FastQC (version 0.11.5)