##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921399_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3391515
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.26137434155532	31.0	31.0	34.0	26.0	34.0
2	30.317228436259313	31.0	31.0	34.0	26.0	34.0
3	30.371980958362265	33.0	31.0	34.0	26.0	34.0
4	33.81695201112187	37.0	35.0	37.0	29.0	37.0
5	33.806554592858944	37.0	35.0	37.0	29.0	37.0
6	33.853302432688636	37.0	35.0	37.0	29.0	37.0
7	33.76170678885395	37.0	35.0	37.0	29.0	37.0
8	33.72673569186632	37.0	35.0	37.0	29.0	37.0
9	35.271254291960965	39.0	37.0	39.0	29.0	39.0
10-11	35.1713075130141	39.0	35.0	39.0	28.0	39.0
12-13	35.05647092818401	39.0	35.0	39.0	27.0	39.0
14-15	36.18920084387066	40.0	36.5	41.0	27.0	41.0
16-17	36.13770704242794	40.0	36.0	41.0	27.0	41.0
18-19	36.08508763782557	40.0	36.5	41.0	27.0	41.0
20-21	36.020650063467215	40.0	36.0	41.0	27.0	41.0
22-23	35.90526151292269	40.0	36.0	41.0	27.0	41.0
24-25	35.80936086085422	40.0	36.0	41.0	27.0	41.0
26-27	35.6658991630584	40.0	36.0	41.0	26.0	41.0
28-29	35.52859223680272	40.0	36.0	41.0	25.5	41.0
30-31	35.39113080732357	40.0	36.0	41.0	25.0	41.0
32-33	35.22422678360556	39.0	35.0	41.0	24.5	41.0
34-35	35.06602668718847	39.0	35.0	41.0	24.0	41.0
36-37	34.90522126542268	39.0	35.0	41.0	24.0	41.0
38-39	35.14299804069863	39.5	35.0	41.0	24.0	41.0
40-41	35.270373712043146	40.0	35.0	41.0	24.0	41.0
42-43	35.12015176108612	40.0	35.0	41.0	23.5	41.0
44-45	35.00926695002086	40.0	35.0	41.0	23.0	41.0
46-47	34.867409402582624	40.0	35.0	41.0	22.0	41.0
48-49	34.730451582847195	39.5	35.0	41.0	21.0	41.0
50-51	33.0209096819563	37.0	32.5	39.5	17.0	40.0
52-53	33.17347601293228	37.5	33.0	39.5	16.5	40.0
54-55	34.041650118015106	39.0	34.0	40.5	17.5	41.0
56-57	34.05632320659056	39.0	34.0	41.0	17.0	41.0
58-59	33.87935480161521	38.0	34.0	41.0	15.0	41.0
60-61	33.63922303159502	38.0	34.0	40.0	13.0	41.0
62-63	33.35283140425444	37.0	33.5	40.0	11.5	41.0
64-65	33.05699812620614	37.0	33.0	40.0	8.0	41.0
66-67	32.72046386349463	36.0	33.0	39.5	7.0	41.0
68-69	32.35528900800969	36.0	33.0	39.0	4.0	41.0
70-71	31.977478796349125	35.0	32.0	39.0	2.0	40.5
72-73	31.627252127736426	35.0	32.0	37.5	2.0	40.0
74-75	31.23650639905765	35.0	32.0	37.0	2.0	39.0
76-77	30.866712074102576	35.0	32.0	36.5	2.0	39.0
78-79	30.528559213212972	35.0	31.0	36.0	2.0	38.5
80-81	30.18691130659897	35.0	31.0	36.0	2.0	37.0
82-83	29.893502166435944	35.0	31.0	35.0	2.0	37.0
84-85	29.668861408544558	35.0	31.0	35.0	2.0	36.5
86-87	29.454631189895963	35.0	31.0	35.0	2.0	36.0
88-89	29.26373036828674	35.0	31.0	35.0	2.0	36.0
90-91	29.027600054842747	34.5	30.0	35.0	2.0	35.5
92-93	28.846773639509188	34.0	30.0	35.0	2.0	35.0
94-95	28.659594900803917	34.0	30.0	35.0	2.0	35.0
96-97	28.428402203734912	34.0	30.0	35.0	2.0	35.0
98-99	28.21174136042447	34.0	30.0	35.0	2.0	35.0
100-101	27.180433965351767	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	129043.0
3	21460.0
4	14288.0
5	10894.0
6	9585.0
7	9841.0
8	10306.0
9	11032.0
10	11932.0
11	12841.0
12	12786.0
13	12690.0
14	13530.0
15	14423.0
16	14252.0
17	14912.0
18	14116.0
19	13449.0
20	13619.0
21	14369.0
22	15688.0
23	17961.0
24	21074.0
25	25073.0
26	29763.0
27	35248.0
28	41532.0
29	49477.0
30	59570.0
31	72720.0
32	90062.0
33	116992.0
34	162712.0
35	237753.0
36	398375.0
37	726800.0
38	771443.0
39	139884.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.53945051126277	17.80708363398648	12.727849852589	24.925616002161753
2	16.096186037400308	19.04455926632008	39.188747456188025	25.6705072400916
3	19.66228213403991	22.951315846532214	28.959951573378458	28.426450446049422
4	13.433760495909022	15.161488458704046	36.088491627759055	35.316259417627876
5	14.583225784374834	36.788381113586745	34.22888797965975	14.39950512237867
6	34.5314352834733	34.681836988733735	16.623812010844258	14.16291571694871
7	29.74874499302127	31.167169023334296	20.462622275264323	18.621463708380112
8	27.219481984472804	34.89121379015431	19.192733331858765	18.696570893514114
9	26.59761580779242	15.689797849142717	19.0798193010923	38.63276704197256
10-11	26.00534449615988	25.08458235838501	27.481672347762075	21.428400797693037
12-13	26.358459787478584	22.36046295916709	27.43374890101318	23.847328352341147
14-15	23.44967265737785	24.02204289878603	24.418028062321646	28.110256381514475
16-17	23.94166719509695	27.210964608934553	25.23309674403378	23.61427145193472
18-19	23.954811640702083	26.63076103877604	26.370640867385752	23.04378645313612
20-21	24.325110274556806	25.83002322873521	26.45810746563763	23.38675903107035
22-23	24.216171617161717	25.597395724556364	26.18369988589849	24.00273277238342
24-25	24.035399331675674	25.793183457638158	26.181267008060626	23.99015020262554
26-27	23.898744451729566	25.967218123971563	26.1596987557186	23.974338668580263
28-29	23.98335039650304	26.097610571330364	26.103626794784702	23.815412237381892
30-31	24.220800293946652	25.841605866429735	26.024172453028765	23.913421386594848
32-33	23.933596112511637	25.925509268583536	25.983601022296916	24.15729359660791
34-35	24.25869226938051	25.93094544915182	25.994787100624762	23.815575180842906
36-37	24.31629543367423	25.75223682602389	26.05933093584682	23.87213680445506
38-39	24.132313701368417	25.950501417012823	26.00251703703223	23.914667844586525
40-41	24.234290019298843	25.795727127037694	26.056047732570224	23.91393512109324
42-43	24.11583942511686	25.7530271781204	26.003297541027205	24.127835855735537
44-45	24.04177870402714	25.87852922301152	26.06173753975926	24.017954533202076
46-47	24.29312278555036	25.78071005076349	25.886544255462645	24.0396229082235
48-49	24.105580450086755	25.919817929291327	25.85729316051702	24.1173084601049
50-51	24.07885452788299	25.99726538457505	25.96967403957231	23.95420604796965
52-53	24.2593685934558	25.829561385614895	25.91359471356659	23.997475307362716
54-55	23.90275814690176	25.782511869489454	26.22060064498414	24.094129338624647
56-57	23.978208880215167	25.916411928271536	26.020661028223778	24.084718163289516
58-59	24.103141878432375	25.76933854825484	26.08924169099005	24.03827788232274
60-61	23.9982745427095	25.85609568015222	26.174758687116096	23.97087109002219
62-63	24.042264847974888	25.88362231970654	26.194653949060243	23.879458883258327
64-65	23.849761415966437	25.73543282752622	26.197946527217752	24.21685922928959
66-67	23.862350777128064	25.82891173710571	26.10744977356691	24.20128771219932
68-69	23.97575994624613	25.702391849357998	26.166785596172122	24.15506260822376
70-71	23.863155379422768	25.602297629030442	26.163664273475394	24.370882718071396
72-73	23.907981024560872	25.774050840340664	26.07626715733371	24.241700977764754
74-75	23.86119738142599	25.813666753905206	26.161267034407626	24.16386883026118
76-77	23.915464039029956	25.64617558429958	26.164563443694018	24.27379693297645
78-79	23.842887269584615	25.600319100609028	26.207437555630243	24.349356074176118
80-81	23.915807582476813	25.763084831264127	26.075682342539974	24.245425243719094
82-83	23.926957464197933	25.726796840234705	26.19079207470255	24.155453620864805
84-85	23.940598684491416	25.72056692472279	26.12866621233661	24.210168178449184
86-87	23.805751730406993	25.81277856287121	26.18131934704656	24.200150359675238
88-89	23.726460724618644	25.65525951880726	26.22589518655073	24.392384570023363
90-91	23.76540418950219	25.677840484967117	26.246667387710694	24.31008793782
92-93	23.90856599433868	25.546187991481272	26.376369210182695	24.168876803997353
94-95	23.834016265045285	25.448240436098992	26.370878026347057	24.34686527250867
96-97	23.815643212674832	25.260554207658963	26.473409919605988	24.450392660060217
98-99	23.70037465358765	25.30466924316429	26.37670601846892	24.618250084779135
100-101	23.899586135800536	25.235002336353997	26.475663939787385	24.389747588058082
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	42.0
1	38.0
2	58.0
3	91.5
4	83.0
5	88.0
6	165.0
7	315.5
8	480.5
9	559.0
10	517.0
11	472.0
12	520.0
13	583.0
14	680.5
15	791.0
16	873.5
17	972.5
18	1048.0
19	1145.5
20	1270.0
21	1399.5
22	1594.5
23	1814.0
24	2078.0
25	2489.0
26	3180.0
27	4319.0
28	6157.0
29	8993.5
30	13158.0
31	18911.0
32	26724.5
33	33770.5
34	40246.0
35	47807.5
36	57384.5
37	67781.0
38	76944.5
39	86053.0
40	94241.0
41	102556.5
42	110037.0
43	116898.5
44	123687.5
45	130940.0
46	136791.5
47	140618.0
48	144390.0
49	146586.0
50	146555.0
51	146314.0
52	145552.0
53	142892.0
54	139870.0
55	134620.0
56	126301.0
57	115341.5
58	104115.5
59	90959.0
60	75776.0
61	62876.0
62	51325.5
63	39223.5
64	28419.0
65	20874.0
66	15620.5
67	11894.5
68	9022.5
69	6969.5
70	5191.0
71	3649.5
72	2646.0
73	1920.5
74	1385.5
75	1011.0
76	731.5
77	485.0
78	308.5
79	240.0
80	197.0
81	153.5
82	129.5
83	107.0
84	84.0
85	69.5
86	58.5
87	47.0
88	39.5
89	36.5
90	39.5
91	31.5
92	20.5
93	17.5
94	17.0
95	17.0
96	12.5
97	9.5
98	6.0
99	3.5
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0493289871930391
2	0.0565234121034405
3	0.0484739121012291
4	0.05487223261580739
5	0.04676376191760909
6	0.01757326740409522
7	0.01468370330073728
8	0.020344890115479363
9	0.02007952198353833
10-11	0.021730701471171437
12-13	0.026300930410155934
14-15	0.0439036831622446
16-17	0.028600787553644907
18-19	0.022836402020925753
20-21	0.026153503670188694
22-23	0.0457317747378384
24-25	0.04111731777686373
26-27	0.030782703305160083
28-29	0.02015323535352195
30-31	0.01248704487522538
32-33	0.00967119414185106
34-35	0.009199428573955887
36-37	0.0013710686816953484
38-39	0.006663688646519329
40-41	0.0035382417592138026
42-43	0.21155737185299195
44-45	0.185683978988741
46-47	0.1917579606753914
48-49	0.1903721493196993
50-51	0.18857354309209895
52-53	0.19308480133509656
54-55	0.19078494419160758
56-57	0.20148812551322934
58-59	0.19874598814983865
60-61	0.2035963278947609
62-63	0.20082470518337675
64-65	0.20222525921306553
66-67	0.19222972624328655
68-69	0.1903721493196993
70-71	0.17508399638509634
72-73	0.1825585321014355
74-75	0.18165922898763534
76-77	0.18031764565393343
78-79	0.2064711493241221
80-81	0.21497767222023195
82-83	0.212677815076743
84-85	0.20770953393984695
86-87	0.22494371984201753
88-89	0.22318934163640733
90-91	0.22211312643464648
92-93	0.22631478852371284
94-95	0.2377403608711741
96-97	0.23747499273923306
98-99	0.2277005998794049
100-101	0.2370032271713379
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3391515.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.35241637080916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.77354106807698	44.79921634665844
2	12.565521886015516	14.66457130018585
3	4.339497735417066	7.596605360917201
4	2.052546099666489	4.790840985120773
5	1.1329889597051601	3.3056321760122716
6	0.7078689987767555	2.478351993156542
7	0.4843101521145489	1.978246735415866
8	0.3332977051553561	1.5558981173328421
9	0.2661858441033977	1.397932848642308
>10	1.2688418227527822	12.96824146982574
>50	0.057075276544939525	2.2452192210230346
>100	0.017058709268866608	1.5830209216529063
>500	8.100751403709916E-4	0.3344341304089015
>1k	4.556672617452497E-4	0.301788393647325
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.897069598689671E-5
2	0.0	0.0	0.0	0.0	5.897069598689671E-5
3	0.0	0.0	0.0	0.0	5.897069598689671E-5
4	0.0	0.0	0.0	0.0	5.897069598689671E-5
5	0.0	0.0	0.0	0.0	5.897069598689671E-5
6	0.0	0.0	0.0	0.0	8.845604398034506E-5
7	0.0	0.0	0.0	0.0	1.1794139197379342E-4
8	0.0	0.0	0.0	0.0	1.1794139197379342E-4
9	0.0	0.0	0.0	0.0	1.1794139197379342E-4
10-11	0.0	0.0	0.0	0.0	1.1794139197379342E-4
12-13	0.0	0.0	0.0	0.0	1.1794139197379342E-4
14-15	0.0	0.0	0.0	0.0	1.1794139197379342E-4
16-17	0.0	0.0	0.0	0.0	1.1794139197379342E-4
18-19	0.0	0.0	0.0	0.0	1.1794139197379342E-4
20-21	0.0	0.0	0.0	2.9485347993448354E-5	1.1794139197379342E-4
22-23	0.0	0.0	0.0	1.4742673996724179E-4	1.3268406597051761E-4
24-25	0.0	0.0	0.0	3.243388279279319E-4	1.7691208796069013E-4
26-27	0.0	0.0	0.0	5.602216118755187E-4	1.7691208796069013E-4
28-29	0.0	0.0	0.0	0.00197551831556104	1.7691208796069013E-4
30-31	0.0	0.0	0.0	0.007857845240253987	1.916547619574143E-4
32-33	0.0	0.0	0.0	0.021376877295250056	2.063974359541385E-4
34-35	0.0	1.4742673996724177E-5	0.0	0.03878797528538132	2.063974359541385E-4
36-37	0.0	2.9485347993448354E-5	0.0	0.06623883426728173	2.063974359541385E-4
38-39	0.0	4.422802199017253E-5	0.0	0.11253083061699565	2.063974359541385E-4
40-41	0.0	5.897069598689671E-5	0.0	0.16706398173087839	2.3588278394758683E-4
42-43	0.0	5.897069598689671E-5	0.0	0.2232777976803877	2.3588278394758683E-4
44-45	0.0	5.897069598689671E-5	0.0	0.29208185722309943	2.3588278394758683E-4
46-47	0.0	5.897069598689671E-5	0.0	0.3741838087108563	2.5062545794431103E-4
48-49	0.0	5.897069598689671E-5	0.0	0.4626840807131916	2.653681319410352E-4
50-51	0.0	5.897069598689671E-5	0.0	0.5563148032663869	2.653681319410352E-4
52-53	0.0	5.897069598689671E-5	0.0	0.6552234031104094	2.801108059377594E-4
54-55	0.0	8.845604398034506E-5	0.0	0.76647162108969	2.9485347993448357E-4
56-57	0.0	8.845604398034506E-5	0.0	0.89635457900083	2.9485347993448357E-4
58-59	0.0	8.845604398034506E-5	0.0	1.0384886990032478	2.9485347993448357E-4
60-61	0.0	8.845604398034506E-5	0.0	1.1925348995950187	2.9485347993448357E-4
62-63	0.0	8.845604398034506E-5	0.0	1.3534217009212697	3.243388279279319E-4
64-65	0.0	8.845604398034506E-5	0.0	1.5274294821046053	3.243388279279319E-4
66-67	0.0	8.845604398034506E-5	0.0	1.731571288937245	3.5382417592138025E-4
68-69	0.0	8.845604398034506E-5	0.0	1.9510602194004742	3.5382417592138025E-4
70-71	0.0	8.845604398034506E-5	0.0	2.1894492579275044	3.5382417592138025E-4
72-73	0.0	8.845604398034506E-5	0.0	2.4384825070801694	3.8330952391482865E-4
74-75	0.0	8.845604398034506E-5	0.0	2.7018308926836534	3.8330952391482865E-4
76-77	0.0	8.845604398034506E-5	0.0	2.993883264558759	3.8330952391482865E-4
78-79	0.0	1.1794139197379342E-4	0.0	3.3297361208781324	3.8330952391482865E-4
80-81	0.0	1.1794139197379342E-4	1.4742673996724177E-5	3.685521072441077	3.8330952391482865E-4
82-83	0.0	1.1794139197379342E-4	2.9485347993448354E-5	4.060043962653858	4.4228021990172533E-4
84-85	0.0	1.1794139197379342E-4	2.9485347993448354E-5	4.446228307998048	4.4228021990172533E-4
86-87	0.0	1.1794139197379342E-4	2.9485347993448354E-5	4.85616310115096	4.4228021990172533E-4
88-89	0.0	1.1794139197379342E-4	2.9485347993448354E-5	5.298885601272588	4.4228021990172533E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2630	0.0	43.846756	1
GTATCAA	4555	0.0	40.631527	1
TCAACGC	5035	0.0	35.158337	4
CAACGCA	5060	0.0	34.981533	5
ATCAACG	5270	0.0	33.49902	3
TATCAAC	5460	0.0	32.16231	2
AACGCAG	5525	0.0	32.116646	6
GTACATG	8400	0.0	26.157042	1
ACGCAGA	6545	0.0	25.808527	7
TACATGG	9025	0.0	24.085545	2
CGCAGAG	6890	0.0	23.967775	8
ACATGGG	9285	0.0	22.591242	3
CATGGGG	7880	0.0	19.814753	4
GTATAGC	970	0.0	19.569294	1
GAGTACA	6470	0.0	19.14358	1
GCAGAGT	8450	0.0	18.812904	9
GTGTAGC	1720	0.0	17.657877	1
AGAGTAC	6575	0.0	17.213404	10-11
GTCTTAG	1080	0.0	17.136723	1
GTATATA	1330	0.0	17.126812	1
>>END_MODULE