FastQCFastQC Report
Mon 15 Aug 2016
SRR2921399_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921399_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3391515
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22800.044.4109231
GTATCAA40150.040.967141
TCAACGC48250.032.2633974
CAACGCA48400.032.2228055
ATCAACG51350.030.4080833
TATCAAC52550.030.3463572
AACGCAG52750.029.6535196
GTACATG82900.024.6580661
TACATGG88650.022.6465282
ACGCAGA66800.022.633597
CGCAGAG68500.021.9330658
ACATGGG90400.021.2102873
CATGGGG73800.019.6144924
GAGTACA64700.017.927951
GCAGAGT85050.016.938359
AGAGTAC68800.016.68904510-11
ATGGGGG45050.015.3041115
TATACAG20350.015.1874145
AGTACAT67950.015.0172012
CAGAGTA76300.014.79932510-11
Produced by FastQC (version 0.11.5)