##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921399_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3391515
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.417889055481105	33.0	31.0	34.0	29.0	34.0
2	31.551959522514274	34.0	31.0	34.0	29.0	34.0
3	31.634184722756643	34.0	31.0	34.0	28.0	34.0
4	35.11480267667989	37.0	35.0	37.0	33.0	37.0
5	34.8555085264255	37.0	35.0	37.0	32.0	37.0
6	34.81395128725658	37.0	35.0	37.0	32.0	37.0
7	34.68144531278794	37.0	35.0	37.0	32.0	37.0
8	34.64130749827142	37.0	35.0	37.0	32.0	37.0
9	36.26899512459771	39.0	37.0	39.0	32.0	39.0
10-11	36.12522574719557	39.0	37.0	39.0	32.0	39.0
12-13	36.05431628637939	39.0	37.0	39.0	32.0	39.0
14-15	37.320226506443284	40.0	38.0	41.0	32.0	41.0
16-17	37.29735516428499	40.0	38.0	41.0	31.5	41.0
18-19	37.25444263109554	40.0	38.0	41.0	31.0	41.0
20-21	37.2157695012406	40.0	38.0	41.0	31.0	41.0
22-23	37.10562565697041	40.0	38.0	41.0	31.0	41.0
24-25	37.04377438991129	40.0	38.0	41.0	31.0	41.0
26-27	36.91391266145071	40.0	37.0	41.0	30.0	41.0
28-29	36.76425270122644	40.0	37.0	41.0	30.0	41.0
30-31	36.63973017958051	40.0	37.0	41.0	30.0	41.0
32-33	36.60528112067911	40.0	37.0	41.0	30.0	41.0
34-35	36.74359850981051	40.0	37.0	41.0	30.0	41.0
36-37	36.72861154970566	40.0	37.0	41.0	30.0	41.0
38-39	36.647719676899555	40.0	37.0	41.0	30.0	41.0
40-41	36.53238169372685	40.0	37.0	41.0	30.0	41.0
42-43	36.438096396448195	40.0	37.0	41.0	30.0	41.0
44-45	36.31283585654199	40.0	36.5	41.0	29.0	41.0
46-47	36.173737990249194	40.0	36.0	41.0	28.5	41.0
48-49	36.026040427360634	40.0	36.0	41.0	28.0	41.0
50-51	35.87489057250226	40.0	35.5	41.0	28.0	41.0
52-53	35.70493112959842	39.5	35.0	41.0	28.0	41.0
54-55	35.49922718902909	39.0	35.0	41.0	27.0	41.0
56-57	35.23781790733639	39.0	35.0	41.0	26.0	41.0
58-59	35.013023235928486	39.0	35.0	41.0	26.0	41.0
60-61	34.73461550369083	38.0	35.0	40.0	26.0	41.0
62-63	34.435528075211224	37.5	34.0	40.0	26.0	41.0
64-65	34.11755130081984	37.0	34.0	40.0	26.0	41.0
66-67	33.774779117886844	36.5	34.0	39.5	25.0	41.0
68-69	33.41037692594607	36.0	34.0	39.0	24.0	41.0
70-71	33.02916838050252	35.5	33.5	39.0	23.5	40.0
72-73	32.63623159561435	35.0	33.0	37.5	22.0	40.0
74-75	32.23142857985296	35.0	33.0	37.0	21.5	39.0
76-77	29.489391319218697	33.0	29.0	34.5	16.5	36.5
78-79	31.13684695482697	34.5	31.5	36.0	19.0	37.5
80-81	31.334498889139514	35.0	32.0	36.0	20.0	37.0
82-83	31.175316635780764	35.0	32.5	35.0	19.5	37.0
84-85	30.947570923318928	35.0	32.0	35.0	18.0	36.5
86-87	30.705645559580304	35.0	32.0	35.0	16.5	36.0
88-89	30.433118090292982	35.0	32.0	35.0	10.0	36.0
90-91	30.258712404338475	35.0	32.0	35.0	6.0	35.0
92-93	30.11947241866835	34.0	32.0	35.0	4.5	35.0
94-95	30.02834559186676	34.0	32.0	35.0	2.0	35.0
96-97	29.849676029738923	34.0	31.5	35.0	2.0	35.0
98-99	29.591825629549035	34.0	31.0	35.0	2.0	35.0
100-101	28.51758992662571	33.5	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	64165.0
3	21527.0
4	11659.0
5	8143.0
6	7040.0
7	7013.0
8	7117.0
9	7898.0
10	8675.0
11	9331.0
12	10054.0
13	10594.0
14	11274.0
15	12400.0
16	13305.0
17	14229.0
18	13578.0
19	12192.0
20	11512.0
21	11608.0
22	12152.0
23	13897.0
24	16476.0
25	19787.0
26	23877.0
27	28564.0
28	34582.0
29	41753.0
30	51025.0
31	63734.0
32	81111.0
33	107774.0
34	153518.0
35	232196.0
36	415501.0
37	825354.0
38	880875.0
39	116022.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.01207076380448	19.440940194261454	13.361026753178335	26.185962288755725
2	15.039994055734565	20.30347008288948	37.63327420519038	27.023261656185575
3	18.07031102794413	24.036159552832643	28.882349940421133	29.011179478802102
4	13.02151966628167	16.29580790968115	35.05522705767563	35.62744536636155
5	14.22214383589521	37.09012585921016	34.365457111897726	14.322273192996903
6	34.47477106622678	35.304467101854854	16.288420139508293	13.932341692410075
7	29.48783194712434	31.34320348730239	20.532306610872343	18.636657954700922
8	27.034957950089726	35.44119528956086	18.98909786563018	18.534748894719243
9	26.285155748979438	15.635224965833853	19.010914001559776	39.06870528362693
10-11	25.88993673751648	25.39474857044024	27.423551683447922	21.291763008595364
12-13	26.17871464895416	22.488874028214347	27.5071233198629	23.8252880029686
14-15	23.226812722083807	24.2300222435807	24.39616721003988	28.146997824295617
16-17	23.79344375016217	27.451399257912108	25.21391638891444	23.541240603011282
18-19	23.81174504431216	26.81961145885885	26.36420918488448	23.004434311944514
20-21	24.189751407198607	25.943906660494353	26.44906810870738	23.417273823599658
22-23	24.025451233972642	25.725728588675445	26.254328645493867	23.99449153185805
24-25	23.864037606305853	25.99658995666311	26.176070577429584	23.963301859601454
26-27	23.75784881620907	26.172726294326736	26.200664320560467	23.868760568903724
28-29	23.86647751649879	26.215555704301792	26.105155855747736	23.812810923451682
30-31	24.077861872807617	25.997985847853368	26.0301886903735	23.893963588965512
32-33	23.80651865006524	26.089389437048467	26.00170127132358	24.102390641562714
34-35	24.08585354518393	26.059917716977125	26.068115645643648	23.78611309219529
36-37	24.131449792642456	25.88806968039074	26.14971023017941	23.830770296787396
38-39	24.008837362832097	26.104187530655597	26.036916600115674	23.850058506396635
40-41	24.08386064075389	25.900150219771422	26.100394103907142	23.91559503556754
42-43	24.005173947672535	25.930601396959972	25.94415108894407	24.12007356642342
44-45	23.946979764147766	25.974339425462738	26.04098184333223	24.037698967057267
46-47	24.0797306081972	25.9529643610828	25.922974928155845	24.044330102564153
48-49	23.933780136547504	26.037328292728603	25.965390567001684	24.063501003722212
50-51	23.963222509508483	26.1331044741219	25.917804414130398	23.985868602239222
52-53	24.167977064765264	25.943818346241727	25.8656957189272	24.02250887006581
54-55	23.74759341827264	25.97048693041284	26.138053867625068	24.143865783689453
56-57	23.863712594378853	26.095175077186056	25.925078992138307	24.116033336296784
58-59	23.92717853690643	25.96725405004367	26.000811512627692	24.104755900422212
60-61	23.88404244088772	26.062511315859776	26.04997909330402	24.003467149948484
62-63	23.899925830572254	26.05748941647977	26.00250377113939	24.040080981808583
64-65	23.681548911248075	25.90049446893258	26.049404419190726	24.368552200628617
66-67	23.704918308410914	26.00108164055996	25.98345741818268	24.310542632846445
68-69	23.786053987892906	25.918597877941057	26.003421248608273	24.29192688555776
70-71	23.71949073723104	25.791906791497652	25.994099839030206	24.494502632241097
72-73	23.74042263467814	25.957367867123676	25.851634727246104	24.450574770952084
74-75	23.651614187597463	26.028217407493425	25.903063951837968	24.41710445307114
76-77	23.701477953646293	25.713692070429637	26.086106479103353	24.498723496820713
78-79	23.637453745790612	25.758161852334112	26.019097408170953	24.585286993704322
80-81	23.722930990001466	25.8846165816996	25.874143513195254	24.518308915103685
82-83	23.66732703050822	25.864664980244385	26.006843502617	24.461164486630395
84-85	23.704906776294077	25.81983858508438	25.874050589415443	24.601204049206096
86-87	23.504730012458737	25.95484046499248	25.931380736346288	24.609048786202493
88-89	23.41618192683768	25.76645012581088	25.9632185532288	24.854149394122636
90-91	23.395631211547563	25.76416824420256	25.975597491717938	24.864603052531944
92-93	23.468964811258886	25.691769508597833	26.06682677684985	24.772438903293427
94-95	23.38118062302715	25.553593043468005	26.119430491442188	24.94579584206266
96-97	23.26879604242317	25.41665609999628	26.188794650571513	25.125753207009033
98-99	23.16470455896105	25.43272248101741	26.07277064901332	25.329802311008216
100-101	23.151539151165863	25.46909321005519	26.125800852640758	25.253566786138197
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	34.0
1	34.5
2	42.5
3	59.5
4	61.5
5	75.0
6	138.0
7	265.0
8	379.0
9	423.0
10	375.0
11	307.0
12	298.5
13	339.5
14	386.0
15	410.0
16	476.0
17	540.0
18	598.0
19	683.0
20	792.5
21	889.0
22	1111.5
23	1433.5
24	1739.0
25	2308.5
26	3290.0
27	4686.0
28	6657.5
29	9651.0
30	14000.0
31	19871.5
32	28038.5
33	35404.0
34	41564.0
35	48795.5
36	57977.0
37	67998.0
38	76934.0
39	85721.0
40	93957.0
41	102308.0
42	110419.5
43	117325.0
44	123436.5
45	130894.5
46	136765.0
47	140652.5
48	145438.5
49	147939.5
50	147690.5
51	146945.0
52	145851.5
53	143895.5
54	140895.5
55	135503.0
56	127499.0
57	116059.5
58	104009.0
59	90801.5
60	75482.5
61	62549.5
62	50790.5
63	38022.5
64	26844.5
65	19366.0
66	14554.5
67	11214.5
68	8416.5
69	6309.5
70	4678.0
71	3293.0
72	2258.5
73	1536.5
74	1102.0
75	767.0
76	503.0
77	304.5
78	178.5
79	101.5
80	60.0
81	38.0
82	21.5
83	15.0
84	15.0
85	10.0
86	6.5
87	5.0
88	2.5
89	3.0
90	3.0
91	1.5
92	1.0
93	0.0
94	1.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15187902751425247
2	3.243388279279319E-4
3	0.006250893774611052
4	0.00448177289500415
5	0.02656629854209697
6	9.435311357903473E-4
7	3.243388279279319E-4
8	0.0020344890115479367
9	0.0
10-11	0.10051555130966544
12-13	3.980521979115528E-4
14-15	0.005882326924692947
16-17	8.845604398034508E-5
18-19	2.3588278394758683E-4
20-21	7.371336998362089E-4
22-23	2.801108059377594E-4
24-25	0.0018428342495905223
26-27	0.0023588278394758686
28-29	0.006073981686650362
30-31	0.01503752747665866
32-33	0.015376608978583318
34-35	0.012221676743284343
36-37	8.845604398034507E-4
38-39	1.6216941396396596E-4
40-41	0.004437544873013978
42-43	0.008668692310073818
44-45	0.008300125460155712
46-47	0.009450054031900197
48-49	0.011425572347461239
50-51	0.005941297620679843
52-53	0.0015332380956593146
54-55	0.0010172445057739683
56-57	0.005793870880712601
58-59	0.009346855313923128
60-61	0.007577734434316228
62-63	0.018575769235872466
64-65	0.03570675642006596
66-67	0.0381687829775189
68-69	0.027716227113841453
70-71	0.042399930414578735
72-73	0.02664001191208059
74-75	0.025873392864250933
76-77	0.2374455073912396
78-79	0.06970336265651192
80-81	0.05509337272575825
82-83	0.02086088370536471
84-85	0.03308256044864906
86-87	0.08046551467412055
88-89	0.26119595519996225
90-91	0.3159355037497991
92-93	0.052071124556429794
94-95	0.00501250915888622
96-97	0.010290386449713477
98-99	0.0015184954216625904
100-101	0.0015184954216625904
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3391515.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.967151737840894
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.51977644296515	43.021465638342185
2	13.180403444777733	15.017000860092278
3	4.655508354529596	7.956331525478204
4	2.1186874824489292	4.827823651909291
5	1.2146975030726321	3.4598928486557545
6	0.7323829329520737	2.5033061803011436
7	0.5003847848222363	1.9953847174992636
8	0.36508898026798026	1.6638463469391704
9	0.26173594998232663	1.341931642010202
>10	1.3689887749002894	13.544195200792238
>50	0.06295785442546238	2.41196930399807
>100	0.018036564641416883	1.6075283046907025
>500	9.352593805235533E-4	0.37996851746648524
>1k	4.1567083371325824E-4	0.26935526182494485
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.9485347993448354E-5	0.0
4	0.0	0.0	0.0	2.9485347993448354E-5	0.0
5	0.0	0.0	0.0	2.9485347993448354E-5	0.0
6	0.0	0.0	0.0	2.9485347993448354E-5	0.0
7	0.0	0.0	0.0	2.9485347993448354E-5	0.0
8	0.0	0.0	0.0	2.9485347993448354E-5	0.0
9	0.0	0.0	0.0	2.9485347993448354E-5	0.0
10-11	0.0	0.0	0.0	7.371336998362088E-5	0.0
12-13	0.0	0.0	0.0	8.845604398034506E-5	0.0
14-15	0.0	1.4742673996724177E-5	0.0	8.845604398034506E-5	2.9485347993448354E-5
16-17	0.0	2.9485347993448354E-5	0.0	8.845604398034506E-5	2.9485347993448354E-5
18-19	0.0	2.9485347993448354E-5	0.0	8.845604398034506E-5	2.9485347993448354E-5
20-21	0.0	4.422802199017253E-5	0.0	1.1794139197379342E-4	2.9485347993448354E-5
22-23	0.0	5.897069598689671E-5	0.0	2.3588278394758683E-4	5.897069598689671E-5
24-25	0.0	5.897069598689671E-5	0.0	4.12794871908277E-4	8.845604398034506E-5
26-27	0.0	5.897069598689671E-5	0.0	6.339349818591396E-4	8.845604398034506E-5
28-29	0.0	5.897069598689671E-5	0.0	0.002078717033538109	8.845604398034506E-5
30-31	0.0	8.845604398034506E-5	0.0	0.008167441394185194	8.845604398034506E-5
32-33	0.0	8.845604398034506E-5	0.0	0.021804414841155056	8.845604398034506E-5
34-35	0.0	8.845604398034506E-5	0.0	0.03967253572518476	1.1794139197379342E-4
36-37	0.0	8.845604398034506E-5	0.0	0.06735927749103277	1.1794139197379342E-4
38-39	0.0	8.845604398034506E-5	0.0	0.11385767127670082	1.4742673996724179E-4
40-41	0.0	8.845604398034506E-5	0.0	0.1684645357605672	1.4742673996724179E-4
42-43	0.0	8.845604398034506E-5	0.0	0.22544497075790612	1.4742673996724179E-4
44-45	0.0	8.845604398034506E-5	0.0	0.2947797665644999	1.4742673996724179E-4
46-47	0.0	8.845604398034506E-5	0.0	0.37707337281421427	1.7691208796069013E-4
48-49	0.0	8.845604398034506E-5	0.0	0.4656621008605299	1.7691208796069013E-4
50-51	0.0	8.845604398034506E-5	0.0	0.5593370514357152	1.7691208796069013E-4
52-53	0.0	8.845604398034506E-5	0.0	0.6588501009136035	1.7691208796069013E-4
54-55	0.0	8.845604398034506E-5	0.0	0.7705258564387891	1.7691208796069013E-4
56-57	0.0	8.845604398034506E-5	0.0	0.9004530423719193	1.7691208796069013E-4
58-59	0.0	8.845604398034506E-5	0.0	1.0416436312385469	1.7691208796069013E-4
60-61	0.0	8.845604398034506E-5	0.0	1.196146854724216	1.7691208796069013E-4
62-63	0.0	8.845604398034506E-5	0.0	1.3570189133764705	1.7691208796069013E-4
64-65	0.0	1.1794139197379342E-4	0.0	1.5312036066477666	1.7691208796069013E-4
66-67	2.9485347993448354E-5	1.1794139197379342E-4	0.0	1.7336205206227895	1.7691208796069013E-4
68-69	2.9485347993448354E-5	1.1794139197379342E-4	0.0	1.952844082954078	1.7691208796069013E-4
70-71	2.9485347993448354E-5	1.1794139197379342E-4	0.0	2.19180808576698	1.916547619574143E-4
72-73	2.9485347993448354E-5	1.1794139197379342E-4	0.0	2.4418733220994158	2.9485347993448357E-4
74-75	2.9485347993448354E-5	1.1794139197379342E-4	0.0	2.7067549457985587	2.9485347993448357E-4
76-77	2.9485347993448354E-5	1.1794139197379342E-4	0.0	3.0003258130953276	2.9485347993448357E-4
78-79	2.9485347993448354E-5	1.1794139197379342E-4	0.0	3.3394220576939806	2.9485347993448357E-4
80-81	2.9485347993448354E-5	1.3268406597051761E-4	0.0	3.6989811338000864	3.095961539312077E-4
82-83	2.9485347993448354E-5	1.4742673996724179E-4	0.0	4.076998037750091	3.243388279279319E-4
84-85	2.9485347993448354E-5	1.4742673996724179E-4	0.0	4.467339817161357	3.243388279279319E-4
86-87	2.9485347993448354E-5	1.4742673996724179E-4	0.0	4.880665425333516	3.390815019246561E-4
88-89	2.9485347993448354E-5	1.7691208796069013E-4	0.0	5.326483886994455	3.5382417592138025E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2280	0.0	44.410923	1
GTATCAA	4015	0.0	40.96714	1
TCAACGC	4825	0.0	32.263397	4
CAACGCA	4840	0.0	32.222805	5
ATCAACG	5135	0.0	30.408083	3
TATCAAC	5255	0.0	30.346357	2
AACGCAG	5275	0.0	29.653519	6
GTACATG	8290	0.0	24.658066	1
TACATGG	8865	0.0	22.646528	2
ACGCAGA	6680	0.0	22.63359	7
CGCAGAG	6850	0.0	21.933065	8
ACATGGG	9040	0.0	21.210287	3
CATGGGG	7380	0.0	19.614492	4
GAGTACA	6470	0.0	17.92795	1
GCAGAGT	8505	0.0	16.93835	9
AGAGTAC	6880	0.0	16.689045	10-11
ATGGGGG	4505	0.0	15.304111	5
TATACAG	2035	0.0	15.187414	5
AGTACAT	6795	0.0	15.017201	2
CAGAGTA	7630	0.0	14.799325	10-11
>>END_MODULE