FastQCFastQC Report
Mon 15 Aug 2016
SRR2921398_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921398_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3070597
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39900.042.3368531
GGTATCA24050.041.4329341
TCAACGC48450.033.6964344
CAACGCA50100.032.29885
ATCAACG51200.031.7907683
TATCAAC51950.031.4236622
AACGCAG54450.030.0606926
GTACATG80800.029.3629781
TACATGG83300.028.3136122
ACATGGG86150.027.0459983
ACGCAGA65000.024.8920827
CATGGGG73750.024.1317334
CGCAGAG67800.023.3761188
ATGGGGG46800.020.7863275
GCAGAGT80400.018.5325979
GAGTACA58750.018.2533261
GTATAGC10250.018.0543651
AGAGTAC65900.017.49780510-11
GTATATG11950.016.6771871
AGTACAT61150.016.4521752
Produced by FastQC (version 0.11.5)