##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921398_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3070597
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.317723231019897	31.0	31.0	34.0	26.0	34.0
2	30.377870817954946	31.0	31.0	34.0	26.0	34.0
3	30.430565782484646	33.0	31.0	34.0	26.0	34.0
4	33.88177641025507	37.0	35.0	37.0	30.0	37.0
5	33.87676402992643	37.0	35.0	37.0	30.0	37.0
6	33.92120066553833	37.0	35.0	37.0	30.0	37.0
7	33.83623380078858	37.0	35.0	37.0	29.0	37.0
8	33.79814739609268	37.0	35.0	37.0	29.0	37.0
9	35.35185340179776	39.0	37.0	39.0	29.0	39.0
10-11	35.24786694574377	39.0	35.0	39.0	29.0	39.0
12-13	35.13515221958466	39.0	35.0	39.0	28.0	39.0
14-15	36.26789481003206	40.0	37.0	41.0	27.0	41.0
16-17	36.22083620872423	40.0	37.0	41.0	27.0	41.0
18-19	36.173100703218296	40.0	36.5	41.0	27.0	41.0
20-21	36.115538932657074	40.0	36.5	41.0	27.0	41.0
22-23	36.00020110095854	40.0	36.0	41.0	27.0	41.0
24-25	35.909023880372445	40.0	36.0	41.0	27.0	41.0
26-27	35.76617152951039	40.0	36.0	41.0	27.0	41.0
28-29	35.62940936241389	40.0	36.0	41.0	26.0	41.0
30-31	35.49153633641927	40.0	36.0	41.0	25.0	41.0
32-33	35.32096983094819	39.5	35.0	41.0	25.0	41.0
34-35	35.16141421358778	39.0	35.0	41.0	24.5	41.0
36-37	34.998628116942726	39.0	35.0	41.0	24.0	41.0
38-39	35.23157239455389	39.5	35.0	41.0	24.5	41.0
40-41	35.35935943401235	40.0	35.0	41.0	24.5	41.0
42-43	35.20480903225008	40.0	35.0	41.0	24.0	41.0
44-45	35.096278834376506	40.0	35.0	41.0	23.0	41.0
46-47	34.957191875065334	40.0	35.0	41.0	23.0	41.0
48-49	34.828107856550375	40.0	35.0	41.0	22.0	41.0
50-51	33.1322930036081	37.0	32.5	39.5	18.0	40.0
52-53	33.287460223533074	37.5	33.5	39.5	17.0	40.0
54-55	34.156930394968796	39.0	34.0	40.5	18.5	41.0
56-57	34.183097781962275	39.0	34.0	41.0	18.0	41.0
58-59	34.01774426927402	38.5	34.0	41.0	18.0	41.0
60-61	33.78427289546626	38.0	34.0	40.5	15.0	41.0
62-63	33.51381864829543	37.5	34.0	40.0	13.0	41.0
64-65	33.23047684212549	37.0	33.5	40.0	12.0	41.0
66-67	32.895089945049776	36.5	33.0	39.5	8.0	41.0
68-69	32.53701218362423	36.0	33.0	39.0	7.0	41.0
70-71	32.16234904808414	35.5	32.5	39.0	3.5	41.0
72-73	31.805464051453185	35.0	32.0	38.5	2.0	40.0
74-75	31.407894132639353	35.0	32.0	37.0	2.0	39.5
76-77	31.026920009366258	35.0	32.0	37.0	2.0	39.0
78-79	30.67499968247217	35.0	31.5	36.0	2.0	39.0
80-81	30.32285936578457	35.0	31.0	36.0	2.0	37.0
82-83	30.017600974663885	35.0	31.0	35.5	2.0	37.0
84-85	29.77814705088294	35.0	31.0	35.0	2.0	36.5
86-87	29.55466949912346	35.0	31.0	35.0	2.0	36.0
88-89	29.354605472486295	35.0	31.0	35.0	2.0	36.0
90-91	29.10551921987809	35.0	30.5	35.0	2.0	36.0
92-93	28.921507283437066	34.0	30.0	35.0	2.0	35.0
94-95	28.7237967079366	34.0	30.0	35.0	2.0	35.0
96-97	28.48462188297585	34.0	30.0	35.0	2.0	35.0
98-99	28.268675765657296	34.0	30.0	35.0	2.0	35.0
100-101	27.2316984286769	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	111888.0
3	18737.0
4	12482.0
5	9512.0
6	8534.0
7	8605.0
8	8952.0
9	9859.0
10	10612.0
11	11480.0
12	11550.0
13	11629.0
14	12305.0
15	13066.0
16	13302.0
17	13977.0
18	12912.0
19	12250.0
20	12499.0
21	12880.0
22	14198.0
23	16121.0
24	18963.0
25	22629.0
26	26837.0
27	31854.0
28	37669.0
29	44539.0
30	54118.0
31	64796.0
32	80660.0
33	105376.0
34	146198.0
35	211597.0
36	351283.0
37	644225.0
38	723586.0
39	138898.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.5705790929618	18.37956280485222	13.23504208060317	24.814816021582804
2	15.710280084525538	19.518132748106403	39.91345419116652	24.85813297620153
3	19.26446448481626	23.60959331251967	29.343157590176787	27.782784612487283
4	13.33102850739494	15.623656898527594	36.80063005816698	34.24468453591048
5	14.329663254640382	37.10216920418201	34.48534835771798	14.082819183459621
6	33.49685789844496	35.23130838001237	17.205485591738846	14.066348129803826
7	28.96442822668142	31.67780561202205	21.038942934225883	18.31882322707065
8	26.465190607353286	35.19806018027503	19.80132743708746	18.535421775284235
9	26.60118756203202	15.947266226208775	19.459691523123713	37.99185468863549
10-11	25.56814415445045	25.362732271213396	27.928344934305493	21.140778640030657
12-13	26.094278973441842	22.7012099430821	27.761601226149494	23.442909857326562
14-15	23.24761593531674	24.401259207104836	24.935171099750328	27.415953757828092
16-17	23.52634861459431	27.491251985334706	25.760755175633815	23.22164422443717
18-19	23.524622298259246	26.958324829930586	26.95058829431215	22.566464577498017
20-21	23.988951620666228	26.151637019237423	26.940751127567637	22.918660232528705
22-23	23.83702858498275	25.890095804590434	26.669021270938924	23.60385433948789
24-25	23.640219983579424	26.067141907653813	26.68397234566614	23.60866576310062
26-27	23.520603509133135	26.342198304886523	26.619412590437925	23.51778559554242
28-29	23.582367060928174	26.508073886328777	26.504897948148493	23.404661104594556
30-31	23.87259588881251	26.218156015756062	26.41845348983212	23.49079460559931
32-33	23.630840875695114	26.26668440669459	26.341563826888816	23.760910890721483
34-35	23.87209835190981	26.307575756095293	26.420920430410117	23.399405461584784
36-37	23.928817138990908	26.17504887165704	26.454331767106304	23.441802222245745
38-39	23.812443420897715	26.32497489308697	26.387345236460952	23.475236449554362
40-41	23.930764210230116	26.144843254960815	26.442941522844755	23.481451011964317
42-43	23.70687418513158	26.168996571637436	26.341997522963172	23.782131720267806
44-45	23.67705231039082	26.198817854597944	26.450079218619898	23.674050616391337
46-47	23.82600467413604	26.125728549345578	26.273718385342953	23.774548391175426
48-49	23.640783620720317	26.26645336284696	26.288477645679208	23.804285370753515
50-51	23.698168047351178	26.294564047213488	26.400148523032733	23.607119382402598
52-53	23.957357341581602	26.134257744864183	26.32639573312381	23.58198918043041
54-55	23.528215050746144	26.03165332415373	26.605057810305194	23.835073814794935
56-57	23.602171222298036	26.15985777541085	26.422009307382826	23.815961694908285
58-59	23.693667617980417	26.075644779924122	26.53551894023692	23.69516866185854
60-61	23.692318536501713	26.097941070365376	26.604943539214332	23.60479685391858
62-63	23.691253880349645	26.08883924477864	26.57716042433314	23.642746450538574
64-65	23.503861555083983	25.931646869133306	26.56166430462476	24.00282727115795
66-67	23.52300975494122	26.104880505110607	26.44588661179876	23.926223128149417
68-69	23.607780259737886	25.984719304668324	26.55280212426202	23.854698311331767
70-71	23.528205660216702	25.819196819614724	26.56116601077419	24.091431509394383
72-73	23.514623547265867	26.017951807798887	26.425564825510406	24.04185981942484
74-75	23.45822444511871	26.13136078458241	26.492135579285303	23.918279191013582
76-77	23.531483458018275	25.817442059060873	26.55672663321616	24.094347849704686
78-79	23.403084670826814	25.826122910380594	26.657059296142627	24.113733122649965
80-81	23.542793753148416	25.99135879286151	26.39192477773214	24.07392267625793
82-83	23.617384924723485	25.994720151378726	26.52660501295159	23.861289910946205
84-85	23.57639762539035	25.991947134878963	26.49355421602212	23.938101023708565
86-87	23.39717106410924	26.124270354631275	26.49847090059948	23.980087680660006
88-89	23.371037109243357	25.891296993245046	26.582100408600372	24.155565488911225
90-91	23.434440824920674	25.860932496743	26.62157647785721	24.083050200479114
92-93	23.498652757400045	25.733688245940726	26.73293261502155	24.03472638163768
94-95	23.5224546737024	25.665655398537957	26.723120749397182	24.088769178362462
96-97	23.42637008464826	25.52569129574464	26.8279207207466	24.2200178988605
98-99	23.286490562219335	25.479078246550035	26.80225256579844	24.432178625432186
100-101	23.49891164688001	25.39166348931103	26.890336181636798	24.219088682172163
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	46.0
1	43.5
2	66.0
3	90.5
4	91.0
5	119.5
6	182.5
7	344.0
8	552.5
9	611.5
10	547.5
11	528.0
12	569.5
13	598.5
14	666.0
15	745.0
16	864.5
17	984.5
18	1092.5
19	1211.0
20	1349.0
21	1556.0
22	1710.5
23	1907.5
24	2212.0
25	2709.5
26	3547.5
27	4770.5
28	6615.5
29	9650.0
30	13902.5
31	19461.5
32	27170.0
33	34195.5
34	40649.0
35	48272.5
36	57367.0
37	67255.0
38	76492.5
39	85135.5
40	92129.0
41	98827.0
42	105500.0
43	111991.0
44	118272.0
45	124257.5
46	128279.0
47	131704.5
48	135285.5
49	135967.0
50	134330.0
51	132302.0
52	129560.0
53	125815.5
54	121089.5
55	114115.0
56	105003.0
57	94226.5
58	83662.0
59	72182.5
60	59454.0
61	48481.5
62	39382.5
63	30225.5
64	21677.0
65	15710.5
66	11672.0
67	8816.0
68	6649.5
69	4932.5
70	3720.5
71	2698.5
72	1819.5
73	1262.5
74	946.0
75	700.0
76	525.5
77	359.5
78	240.5
79	175.5
80	127.5
81	94.0
82	74.5
83	67.5
84	63.0
85	53.0
86	40.5
87	35.5
88	32.0
89	27.5
90	27.0
91	24.0
92	19.0
93	20.0
94	20.0
95	16.5
96	16.0
97	12.5
98	7.0
99	6.0
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04842706483462336
2	0.05542896055718155
3	0.04751519004284834
4	0.05451708576540653
5	0.0473197883017537
6	0.016869683647837863
7	0.014264327099909236
8	0.02022408020329597
9	0.019865843677955786
10-11	0.021738443696779488
12-13	0.02685145592208942
14-15	0.0444213291421831
16-17	0.029326544642621615
18-19	0.024343800244708114
20-21	0.026884022878938523
22-23	0.046668449164771544
24-25	0.04158800389631072
26-27	0.030938609006652452
28-29	0.020777718469730803
30-31	0.013466436657106094
32-33	0.010665678368082819
34-35	0.010291158364318079
36-37	0.0017748991482763776
38-39	0.007408982683172035
40-41	0.004331405260931343
42-43	0.2101220055904438
44-45	0.1843615427227995
46-47	0.1908260836573474
48-49	0.1888232158111273
50-51	0.18734141927449288
52-53	0.19124945409638583
54-55	0.18993049234399695
56-57	0.20022165070831505
58-59	0.19745345937614087
60-61	0.2023547863819316
62-63	0.1992446420028418
64-65	0.20054732027680613
66-67	0.19027244539091256
68-69	0.18937685407756213
70-71	0.17286540695506444
72-73	0.18099086268891684
74-75	0.17999757050501908
76-77	0.17819010439989358
78-79	0.2046181898829446
80-81	0.21355781953802472
82-83	0.21148981777810633
84-85	0.2066699081644384
86-87	0.2235884422475499
88-89	0.22293710311056775
90-91	0.22182982657769806
92-93	0.2257541448780156
94-95	0.23713629629677876
96-97	0.23687576064198593
98-99	0.22730107532834817
100-101	0.23620813802657917
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3070597.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.83311733678383
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.98796960857581	50.258934799467426
2	11.307550775776491	14.209773293720104
3	3.5434248450312613	6.67933287185773
4	1.647027240076757	4.139514233304883
5	0.897828822037269	2.820669186170706
6	0.5841916231082487	2.2023948481156066
7	0.3979950658555197	1.7505089470652613
8	0.2820947797002071	1.4179915514397836
9	0.20992521806693681	1.1871230272874795
>10	1.0872044995832397	11.756481884932866
>50	0.041710397153952014	1.7672103155615115
>100	0.011932069236084517	1.2227778303690344
>500	9.368638351063553E-4	0.42648471526365
>1k	2.0819196310391995E-4	0.16080249544394573
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.256695684910785E-5
2	0.0	0.0	0.0	0.0	6.51339136982157E-5
3	0.0	0.0	0.0	0.0	6.51339136982157E-5
4	0.0	0.0	0.0	0.0	6.51339136982157E-5
5	0.0	0.0	0.0	0.0	6.51339136982157E-5
6	0.0	0.0	0.0	0.0	9.770087054732354E-5
7	0.0	0.0	0.0	0.0	9.770087054732354E-5
8	0.0	0.0	0.0	0.0	9.770087054732354E-5
9	0.0	0.0	0.0	6.51339136982157E-5	1.302678273964314E-4
10-11	0.0	0.0	0.0	6.51339136982157E-5	1.302678273964314E-4
12-13	0.0	0.0	0.0	6.51339136982157E-5	2.768191332174167E-4
14-15	0.0	0.0	0.0	6.51339136982157E-5	3.2566956849107843E-4
16-17	0.0	0.0	0.0	8.141739212276961E-5	3.9080348218929414E-4
18-19	0.0	0.0	0.0	1.302678273964314E-4	4.7222087431206375E-4
20-21	0.0	0.0	0.0	1.7911826267009314E-4	5.210713095857254E-4
22-23	0.0	0.0	0.0	2.11685219519201E-4	6.024887017084951E-4
24-25	0.0	0.0	0.0	3.582365253401863E-4	6.839060938312648E-4
26-27	0.0	0.0	0.0	7.816069643785883E-4	7.490400075294804E-4
28-29	0.0	0.0	0.0	0.002523939155805858	7.816069643785883E-4
30-31	0.0	0.0	0.0	0.009900354882128783	7.816069643785883E-4
32-33	0.0	0.0	0.0	0.02686773940051397	7.816069643785883E-4
34-35	0.0	0.0	0.0	0.04950177441064392	8.141739212276961E-4
36-37	0.0	0.0	0.0	0.08561852955630453	8.141739212276961E-4
38-39	0.0	0.0	0.0	0.14560686407236118	8.141739212276961E-4
40-41	0.0	0.0	0.0	0.21500704911781	8.141739212276961E-4
42-43	0.0	0.0	0.0	0.28601929852728963	8.3045739965225E-4
44-45	0.0	0.0	0.0	0.37127959155825396	8.630243565013579E-4
46-47	0.0	0.0	0.0	0.46963180124255965	9.932921838977891E-4
48-49	0.0	0.0	0.0	0.5767445223192753	0.0010421426191714511
50-51	0.0	0.0	0.0	0.6886445860528099	0.0010421426191714511
52-53	0.0	0.0	0.0	0.807025474199317	0.001074709576020559
54-55	0.0	0.0	0.0	0.9419666599035953	0.0011398434897187744
56-57	0.0	0.0	0.0	1.0989231084378706	0.0011886939249924362
58-59	0.0	0.0	0.0	1.2649006040193487	0.0012049774034169902
60-61	0.0	0.0	0.0	1.4396060440363878	0.0012375443602660981
62-63	0.0	0.0	0.0	1.6239838702376117	0.001270111317115206
64-65	0.0	0.0	0.0	1.8272993818465921	0.001270111317115206
66-67	0.0	0.0	0.0	2.06117898245846	0.001270111317115206
68-69	0.0	0.0	0.0	2.318718477221205	0.001270111317115206
70-71	3.256695684910785E-5	0.0	0.0	2.5929485373691175	0.001270111317115206
72-73	3.256695684910785E-5	0.0	0.0	2.873317468883087	0.0014329461013607451
74-75	3.256695684910785E-5	0.0	0.0	3.172314699714746	0.0014329461013607451
76-77	3.256695684910785E-5	0.0	0.0	3.5136815414070943	0.0014329461013607451
78-79	3.256695684910785E-5	1.6283478424553924E-5	0.0	3.895187157415968	0.001449229579785299
80-81	3.256695684910785E-5	3.256695684910785E-5	0.0	4.287016498746009	0.001465513058209853
82-83	3.256695684910785E-5	3.256695684910785E-5	0.0	4.70276626988172	0.001465513058209853
84-85	3.256695684910785E-5	3.256695684910785E-5	0.0	5.1332525889916525	0.001481796536634407
86-87	3.256695684910785E-5	3.256695684910785E-5	0.0	5.602477303273599	0.0015143634934835148
88-89	3.256695684910785E-5	3.256695684910785E-5	0.0	6.115553424952868	0.0015306469719080687
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3990	0.0	42.336853	1
GGTATCA	2405	0.0	41.432934	1
TCAACGC	4845	0.0	33.696434	4
CAACGCA	5010	0.0	32.2988	5
ATCAACG	5120	0.0	31.790768	3
TATCAAC	5195	0.0	31.423662	2
AACGCAG	5445	0.0	30.060692	6
GTACATG	8080	0.0	29.362978	1
TACATGG	8330	0.0	28.313612	2
ACATGGG	8615	0.0	27.045998	3
ACGCAGA	6500	0.0	24.892082	7
CATGGGG	7375	0.0	24.131733	4
CGCAGAG	6780	0.0	23.376118	8
ATGGGGG	4680	0.0	20.786327	5
GCAGAGT	8040	0.0	18.532597	9
GAGTACA	5875	0.0	18.253326	1
GTATAGC	1025	0.0	18.054365	1
AGAGTAC	6590	0.0	17.497805	10-11
GTATATG	1195	0.0	16.677187	1
AGTACAT	6115	0.0	16.452175	2
>>END_MODULE