FastQCFastQC Report
Mon 15 Aug 2016
SRR2921398_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921398_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3070597
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22650.049.0981031
GTATCAA42150.040.7030261
TCAACGC50050.033.091424
CAACGCA52150.031.997695
ATCAACG52300.031.6677913
TATCAAC53750.030.9900822
AACGCAG55750.029.9285436
GTACATG78400.026.126321
TACATGG80700.024.2280082
ACATGGG81700.023.5248583
ACGCAGA69550.023.4437
CGCAGAG71850.022.6264068
CATGGGG68000.021.564714
GTATAGG7900.019.8519841
GCAGAGT84800.018.1620719
ATGGGGG41400.017.6842355
AGAGTAC70050.017.43978910-11
GTATTAA16750.017.307411
CAGAGTA78900.015.5137410-11
ATTATAC10150.015.4291483
Produced by FastQC (version 0.11.5)