##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921398_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3070597
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.424774726217734	33.0	31.0	34.0	29.0	34.0
2	31.55877798356476	34.0	31.0	34.0	29.0	34.0
3	31.63700935029898	34.0	31.0	34.0	28.0	34.0
4	35.12056775929892	37.0	35.0	37.0	33.0	37.0
5	34.86158424566949	37.0	35.0	37.0	32.0	37.0
6	34.81755762804432	37.0	35.0	37.0	32.0	37.0
7	34.68504658866012	37.0	35.0	37.0	32.0	37.0
8	34.649129143290374	37.0	35.0	37.0	32.0	37.0
9	36.271603860747604	39.0	37.0	39.0	32.0	39.0
10-11	36.123548612859324	39.0	37.0	39.0	32.0	39.0
12-13	36.05910055927235	39.0	37.0	39.0	32.0	39.0
14-15	37.313233713183465	40.0	38.0	41.0	32.0	41.0
16-17	37.29533914740358	40.0	38.0	41.0	31.5	41.0
18-19	37.25957216137448	40.0	38.0	41.0	31.0	41.0
20-21	37.22367653586583	40.0	38.0	41.0	31.0	41.0
22-23	37.115648031962515	40.0	38.0	41.0	31.0	41.0
24-25	37.056653478134706	40.0	38.0	41.0	31.0	41.0
26-27	36.92491867216701	40.0	37.0	41.0	30.0	41.0
28-29	36.77667323325073	40.0	37.0	41.0	30.0	41.0
30-31	36.650187080883626	40.0	37.0	41.0	30.0	41.0
32-33	36.60417860109939	40.0	37.0	41.0	30.0	41.0
34-35	36.734636619523826	40.0	37.0	41.0	30.0	41.0
36-37	36.718241273602494	40.0	37.0	41.0	30.0	41.0
38-39	36.636987204768324	40.0	37.0	41.0	30.0	41.0
40-41	36.52580556810288	40.0	37.0	41.0	30.0	41.0
42-43	36.428728680448785	40.0	37.0	41.0	30.0	41.0
44-45	36.300436201820034	40.0	36.5	41.0	29.0	41.0
46-47	36.15920861643518	40.0	36.0	41.0	28.5	41.0
48-49	36.01533447730197	40.0	36.0	41.0	28.0	41.0
50-51	35.87065235196934	40.0	35.5	41.0	28.0	41.0
52-53	35.70694379627154	39.5	35.0	41.0	28.0	41.0
54-55	35.50717857146346	39.0	35.0	41.0	27.0	41.0
56-57	35.25523375421783	39.0	35.0	41.0	26.0	41.0
58-59	35.04135531298962	39.0	35.0	41.0	26.0	41.0
60-61	34.77567489318852	38.0	35.0	40.0	26.0	41.0
62-63	34.48832621148266	38.0	34.0	40.0	26.0	41.0
64-65	34.18430210802655	37.0	34.0	40.0	26.0	41.0
66-67	33.851540270507655	37.0	34.0	39.5	25.0	41.0
68-69	33.490753101107046	36.0	34.0	39.0	24.0	41.0
70-71	33.110935267636876	36.0	34.0	39.0	23.5	40.5
72-73	32.712905340557555	35.0	33.0	38.0	22.0	40.0
74-75	32.30239038206577	35.0	33.0	37.0	21.5	39.0
76-77	29.538102199669964	33.0	29.0	34.5	15.0	36.5
78-79	31.191056494877053	34.5	31.5	36.0	19.0	38.0
80-81	31.376025737014658	35.0	32.0	36.0	20.0	37.0
82-83	31.206621383398733	35.0	32.5	35.5	19.5	37.0
84-85	30.968547484414266	35.0	32.0	35.0	18.0	36.5
86-87	30.722052096058192	35.0	32.0	35.0	16.0	36.0
88-89	30.440599987559423	35.0	32.0	35.0	10.0	36.0
90-91	30.259547898991627	35.0	32.0	35.0	4.5	35.5
92-93	30.117071045142033	34.0	32.0	35.0	3.5	35.0
94-95	30.02509984214796	34.0	32.0	35.0	2.0	35.0
96-97	29.842206906344273	34.0	31.5	35.0	2.0	35.0
98-99	29.58832924020964	34.0	31.0	35.0	2.0	35.0
100-101	28.51299144759146	33.5	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	57222.0
3	19528.0
4	10495.0
5	7245.0
6	6358.0
7	6234.0
8	6552.0
9	6991.0
10	7858.0
11	8617.0
12	8981.0
13	9709.0
14	10532.0
15	11686.0
16	12331.0
17	13498.0
18	12588.0
19	11078.0
20	10669.0
21	10392.0
22	11230.0
23	12484.0
24	14961.0
25	17815.0
26	21564.0
27	26160.0
28	31415.0
29	38368.0
30	46164.0
31	57592.0
32	73185.0
33	96525.0
34	138830.0
35	207147.0
36	365661.0
37	727556.0
38	822194.0
39	113180.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.521930622769545	19.898645761784273	13.77685107815261	25.80257253729357
2	14.859188070025745	20.835410841907976	38.320580605975735	25.984820482090548
3	17.801427887015777	24.69105927512636	29.22452401323471	28.28298882462315
4	12.999338210772052	16.879338106553277	35.72860649074537	34.3927171919293
5	14.038904751278992	37.46837339831032	34.48375385407216	14.008967996338528
6	33.397056883853246	35.93786790987297	16.898378306201973	13.766696900071812
7	28.86079148358555	31.873830028645976	20.943936470799727	18.321442016968735
8	26.37066593975704	35.826939492264046	19.487398441899177	18.314996126079738
9	26.28781959990191	15.997117172979717	19.31888163767502	38.39618158944336
10-11	25.485870893646457	25.684629961601065	27.82375935320011	21.005739791552365
12-13	25.986094829827998	22.84447798130942	27.832860486334116	23.33656670252846
14-15	23.08731632344986	24.571091622587335	24.92145188380318	27.42014017015963
16-17	23.382799899367956	27.74464537381629	25.708198010644523	23.164356716171227
18-19	23.424197111593212	27.16100886948108	26.93893395901205	22.475860059913654
20-21	23.851309861170886	26.27040192980236	26.93801337111781	22.940274837908945
22-23	23.670816901903837	26.028621870266583	26.71573759952895	23.58482362830063
24-25	23.521137700495792	26.249155483660957	26.683459562302385	23.546247253540862
26-27	23.36732138280164	26.486154820189995	26.667412475724568	23.479111321283792
28-29	23.496784025276387	26.621434414129475	26.53077775014815	23.351003810445984
30-31	23.772136429032326	26.406424006444073	26.36346136244674	23.457978202076866
32-33	23.587651706771926	26.389751371979198	26.340583728555522	23.68201319269335
34-35	23.790050325203545	26.43499788778108	26.447163096196995	23.327788690818377
36-37	23.75683626476231	26.348958321459868	26.527491774336582	23.36671363944124
38-39	23.724989142971314	26.465210367448748	26.407127095870614	23.40267339370932
40-41	23.867350305315245	26.309220881875618	26.413489563198418	23.409939249610723
42-43	23.642716847828833	26.24450158298064	26.342569680542603	23.77021188864793
44-45	23.63943409669211	26.32647328244275	26.394625954198474	23.639466666666667
46-47	23.72855437808979	26.316416877924905	26.22653950622652	23.728489237758787
48-49	23.575961400817956	26.42028479713428	26.29246010180702	23.71129370024075
50-51	23.670359716096318	26.402782041146004	26.35287009949005	23.573988143267627
52-53	23.844673524745982	26.26341824676384	26.251270542403145	23.640637686087036
54-55	23.42278366738662	26.186816250283336	26.52862597408688	23.861774108243168
56-57	23.50070226720351	26.29571759494856	26.30263848912809	23.90094164871984
58-59	23.545716024963106	26.239858046028864	26.435604963829164	23.77882096517887
60-61	23.625161749029346	26.262049549512874	26.465870425551802	23.646918275905982
62-63	23.585593690716923	26.27013996018262	26.41557894160812	23.728687407492338
64-65	23.347998102615158	26.125806649187094	26.43672169430211	24.089473553895633
66-67	23.37082403803315	26.33745217960027	26.284282655316016	24.007441127050566
68-69	23.403611764401198	26.189290646683077	26.36544686426459	24.041650724651138
70-71	23.327486127003407	26.01962289428894	26.409389588149896	24.24350139055776
72-73	23.378545769345724	26.203890034017235	26.22690480630862	24.19065939032842
74-75	23.278233751291406	26.28498776225399	26.249057147105898	24.1877213393487
76-77	23.286559525816784	25.959912035709337	26.4005496054322	24.35297883304168
78-79	23.20504278279902	26.03359106810589	26.411630312164274	24.349735836930815
80-81	23.33894830893325	26.151981026281117	26.185071311928	24.323999352857633
82-83	23.327707244917587	26.11839476834039	26.281721260571718	24.2721767261703
84-85	23.27403110251849	26.138351818347815	26.23826846759884	24.34934861153485
86-87	23.101239211493834	26.18640974159735	26.238673698208654	24.473677348700164
88-89	22.99723853872756	26.03740919618522	26.303400251231913	24.661952013855306
90-91	23.02419026815675	25.937914814109376	26.34765273408543	24.690242183648447
92-93	23.00753092403501	25.834270795573495	26.41918206589253	24.739016214498964
94-95	22.963165008386394	25.790291325375758	26.411812600758843	24.834731065479
96-97	22.848004101249934	25.662972165273988	26.49480088727963	24.99422284619645
98-99	22.665494454818088	25.63802525835368	26.445079763436404	25.25140052339183
100-101	22.622497438572974	25.641398913681375	26.475352656230765	25.260750991514886
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	39.0
1	45.5
2	60.5
3	73.5
4	68.5
5	71.0
6	135.0
7	323.0
8	471.5
9	475.5
10	421.5
11	350.5
12	343.5
13	389.0
14	421.0
15	463.5
16	527.0
17	577.5
18	639.0
19	758.0
20	887.0
21	1002.0
22	1204.5
23	1532.0
24	1974.5
25	2541.0
26	3434.0
27	4888.5
28	6999.5
29	10146.5
30	14443.0
31	19993.0
32	27861.0
33	35042.5
34	41050.0
35	48477.0
36	57659.0
37	67346.5
38	76714.0
39	85189.5
40	92602.5
41	99647.0
42	105905.0
43	112056.5
44	118508.5
45	125019.0
46	128914.0
47	131811.0
48	135661.0
49	136968.5
50	135212.5
51	133145.0
52	130450.0
53	126751.0
54	122750.5
55	115616.5
56	105414.0
57	94069.0
58	83267.5
59	71819.0
60	58914.5
61	47984.5
62	38776.0
63	29271.0
64	20675.5
65	14666.0
66	10793.5
67	8140.0
68	6092.0
69	4486.0
70	3228.0
71	2339.0
72	1575.5
73	1017.0
74	706.0
75	490.0
76	336.5
77	211.5
78	117.0
79	63.5
80	33.0
81	21.0
82	16.5
83	8.5
84	5.5
85	4.5
86	5.5
87	4.0
88	1.5
89	2.0
90	1.0
91	1.5
92	1.5
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14717007800111837
2	3.908034821892941E-4
3	0.006057453973934059
4	0.004331405260931343
5	0.025793029824493413
6	0.0013678121876625295
7	2.931026116419706E-4
8	0.0020842852383429022
9	0.0
10-11	0.09978515578566644
12-13	4.070869606138481E-4
14-15	0.006057453973934059
16-17	1.465513058209853E-4
18-19	3.7452000376474024E-4
20-21	7.653234859540343E-4
22-23	2.605356547928628E-4
24-25	0.001709765234578162
26-27	0.0034520974260054313
28-29	0.007213580942077388
30-31	0.01589267494236463
32-33	0.015908958420789182
34-35	0.011919506206773471
36-37	7.816069643785882E-4
38-39	1.7911826267009314E-4
40-41	0.0053409809232536866
42-43	0.009167598353023857
44-45	0.009265299223571182
46-47	0.009851504446855123
48-49	0.012261459253689103
50-51	0.006171438322905937
52-53	0.001888883497248255
54-55	9.444417486241275E-4
56-57	0.006008603538660397
58-59	0.009965488795827
60-61	0.008272007039673393
62-63	0.018921401929331658
64-65	0.036116755145660595
66-67	0.038087056035031625
68-69	0.028610071591941243
70-71	0.043151217825067895
72-73	0.02701429070633496
74-75	0.02577674634606886
76-77	0.2380156041317047
78-79	0.06944903548072248
80-81	0.056031449258890045
82-83	0.021901278481025025
84-85	0.0338207846877985
86-87	0.08197103038920445
88-89	0.26340154699558427
90-91	0.3171370257966122
92-93	0.051602343127411375
94-95	0.004787342656818854
96-97	0.01020974097219531
98-99	0.0015794974071817304
100-101	0.0014003791445116372
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3070597.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.748907036706754
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.27769711679731	48.953111493493125
2	11.564086214106911	14.281393691986995
3	3.7093047052399344	6.8713653424403915
4	1.7324546897513087	4.279087343310408
5	0.9945174722640813	3.070518347060767
6	0.5860622674349749	2.1713222681738302
7	0.42549290959485564	1.8391605483545428
8	0.29452725588075984	1.4549388914525905
9	0.21820843200195061	1.21267189640826
>10	1.1394980698983583	12.178781732377885
>50	0.04395700217203582	1.817244342034902
>100	0.01297760751723557	1.279314291363739
>500	0.0010576150786881444	0.4687705964186945
>1k	1.5864226157231775E-4	0.12231921512386561
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.256695684910785E-5	0.0
3	0.0	0.0	0.0	3.256695684910785E-5	0.0
4	0.0	0.0	0.0	3.256695684910785E-5	0.0
5	0.0	0.0	0.0	3.256695684910785E-5	0.0
6	0.0	0.0	0.0	3.256695684910785E-5	1.302678273964314E-4
7	0.0	0.0	0.0	3.256695684910785E-5	1.302678273964314E-4
8	0.0	0.0	0.0	3.256695684910785E-5	1.302678273964314E-4
9	0.0	0.0	0.0	1.302678273964314E-4	1.9540174109464707E-4
10-11	0.0	0.0	0.0	1.6283478424553922E-4	1.9540174109464707E-4
12-13	0.0	0.0	0.0	1.6283478424553922E-4	2.442521763683089E-4
14-15	0.0	0.0	0.0	1.6283478424553922E-4	2.768191332174167E-4
16-17	0.0	0.0	0.0	1.6283478424553922E-4	2.931026116419706E-4
18-19	0.0	0.0	0.0	1.6283478424553922E-4	3.582365253401863E-4
20-21	0.0	0.0	0.0	2.442521763683089E-4	3.9080348218929414E-4
22-23	0.0	0.0	0.0	3.093860900665245E-4	4.885043527366176E-4
24-25	0.0	0.0	0.0	4.5593739588750985E-4	5.536382664348334E-4
26-27	0.0	0.0	0.0	8.630243565013579E-4	6.187721801330491E-4
28-29	0.0	0.0	0.0	0.002654206983202289	6.676226154067108E-4
30-31	0.0	0.0	0.0	0.010209740972195309	6.839060938312648E-4
32-33	0.0	0.0	0.0	0.027633062886468003	8.141739212276961E-4
34-35	1.6283478424553924E-5	0.0	0.0	0.05093472051200467	9.118747917750197E-4
36-37	3.256695684910785E-5	0.0	0.0	0.08723059392033536	9.118747917750197E-4
38-39	3.256695684910785E-5	0.0	0.0	0.14728406235009023	9.281582701995735E-4
40-41	3.256695684910785E-5	0.0	0.0	0.21728673609724752	9.444417486241275E-4
42-43	3.256695684910785E-5	3.256695684910785E-5	0.0	0.28916200986322854	9.444417486241275E-4
44-45	3.256695684910785E-5	3.256695684910785E-5	0.0	0.3745362872431648	9.444417486241275E-4
46-47	3.256695684910785E-5	3.256695684910785E-5	0.0	0.47264424475110217	9.770087054732353E-4
48-49	3.256695684910785E-5	3.256695684910785E-5	0.0	0.5799035171336389	0.0011072765328696667
50-51	3.256695684910785E-5	3.256695684910785E-5	0.0	0.6915756121692296	0.0011398434897187746
52-53	3.256695684910785E-5	3.256695684910785E-5	0.0	0.8108032411938134	0.0012049774034169902
54-55	3.256695684910785E-5	3.256695684910785E-5	0.0	0.9460375295097339	0.001270111317115206
56-57	3.256695684910785E-5	3.256695684910785E-5	0.0	1.1029776945655845	0.0012863947955397599
58-59	3.256695684910785E-5	3.256695684910785E-5	0.0	1.2700787501583568	0.0013026782739643137
60-61	3.256695684910785E-5	3.256695684910785E-5	0.0	1.4439862997325927	0.0013026782739643137
62-63	3.256695684910785E-5	3.256695684910785E-5	0.0	1.6285269607180624	0.0014003791445116372
64-65	3.256695684910785E-5	3.256695684910785E-5	0.0	1.8329497488599122	0.0014003791445116372
66-67	3.256695684910785E-5	3.256695684910785E-5	0.0	2.0673341373029412	0.0014003791445116372
68-69	3.256695684910785E-5	3.256695684910785E-5	0.0	2.324841065108837	0.0014003791445116372
70-71	6.51339136982157E-5	4.885043527366177E-5	0.0	2.6000807009190723	0.001481796536634407
72-73	6.51339136982157E-5	6.51339136982157E-5	0.0	2.882012846361799	0.0016609147993045
74-75	6.51339136982157E-5	8.141739212276961E-5	0.0	3.183224630259197	0.0016609147993045
76-77	6.51339136982157E-5	9.770087054732354E-5	0.0	3.5272782458915968	0.0016609147993045
78-79	6.51339136982157E-5	9.770087054732354E-5	0.0	3.911340368013126	0.0016609147993045
80-81	6.51339136982157E-5	9.770087054732354E-5	0.0	4.307387130255126	0.0017260487130027159
82-83	6.51339136982157E-5	9.770087054732354E-5	0.0	4.7273217553459475	0.0018237495835500394
84-85	6.51339136982157E-5	9.770087054732354E-5	0.0	5.160152895349015	0.0018237495835500394
86-87	6.51339136982157E-5	9.770087054732354E-5	0.0	5.633660164456619	0.001888883497248255
88-89	6.51339136982157E-5	9.770087054732354E-5	0.0	6.152077267059142	0.0019540174109464706
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2265	0.0	49.098103	1
GTATCAA	4215	0.0	40.703026	1
TCAACGC	5005	0.0	33.09142	4
CAACGCA	5215	0.0	31.99769	5
ATCAACG	5230	0.0	31.667791	3
TATCAAC	5375	0.0	30.990082	2
AACGCAG	5575	0.0	29.928543	6
GTACATG	7840	0.0	26.12632	1
TACATGG	8070	0.0	24.228008	2
ACATGGG	8170	0.0	23.524858	3
ACGCAGA	6955	0.0	23.443	7
CGCAGAG	7185	0.0	22.626406	8
CATGGGG	6800	0.0	21.56471	4
GTATAGG	790	0.0	19.851984	1
GCAGAGT	8480	0.0	18.162071	9
ATGGGGG	4140	0.0	17.684235	5
AGAGTAC	7005	0.0	17.439789	10-11
GTATTAA	1675	0.0	17.30741	1
CAGAGTA	7890	0.0	15.51374	10-11
ATTATAC	1015	0.0	15.429148	3
>>END_MODULE