FastQCFastQC Report
Mon 15 Aug 2016
SRR2921397_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921397_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1687031
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15350.056.2731321
GTATCAA27950.044.4895021
TCAACGC35200.034.3772544
ATCAACG36550.033.1084863
TATCAAC37050.032.9217532
CAACGCA37400.032.353145
AACGCAG38900.031.3384066
GTACATG43850.027.3835031
ACGCAGA45800.026.104697
TACATGG46250.025.8599012
CGCAGAG47050.025.1108768
ACATGGG48100.025.059663
CATGGGG39350.022.430654
GCAGAGT54700.020.558119
GAGTACT28050.019.3690212-13
GTATTAG4700.019.1864151
TAAGGTG7450.019.1078975
TATACCG1507.7765854E-418.9805135
GAGTACA33400.018.8991931
CAGAGTA48600.018.84298110-11
Produced by FastQC (version 0.11.5)