##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921397_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1687031
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.166969664457856	31.0	31.0	34.0	26.0	34.0
2	30.228742684633538	31.0	31.0	34.0	26.0	34.0
3	30.281249722145	33.0	31.0	34.0	26.0	34.0
4	33.72891013858074	37.0	35.0	37.0	28.0	37.0
5	33.71980420039703	37.0	35.0	37.0	28.0	37.0
6	33.760108142648235	37.0	35.0	37.0	29.0	37.0
7	33.67461712321825	37.0	35.0	37.0	28.0	37.0
8	33.63739966841155	37.0	35.0	37.0	28.0	37.0
9	35.170773981035325	39.0	35.0	39.0	28.0	39.0
10-11	35.06491759783904	39.0	35.0	39.0	28.0	39.0
12-13	34.94657626326961	39.0	35.0	39.0	27.0	39.0
14-15	36.05736142370828	40.0	36.0	41.0	27.0	41.0
16-17	36.01257564324544	40.0	36.0	41.0	27.0	41.0
18-19	35.96728098061031	40.0	36.0	41.0	27.0	41.0
20-21	35.91182971741479	40.0	36.0	41.0	27.0	41.0
22-23	35.79424740861312	40.0	36.0	41.0	27.0	41.0
24-25	35.699280866800905	40.0	36.0	41.0	26.5	41.0
26-27	35.551533729967026	40.0	36.0	41.0	25.5	41.0
28-29	35.407375738797924	40.0	36.0	41.0	25.0	41.0
30-31	35.256656516685226	39.5	35.0	41.0	24.5	41.0
32-33	35.06972841637172	39.0	35.0	41.0	24.0	41.0
34-35	34.905988390254834	39.0	35.0	41.0	24.0	41.0
36-37	34.73094033245388	39.0	35.0	41.0	23.0	41.0
38-39	34.94073375059498	39.0	35.0	41.0	23.5	41.0
40-41	35.048657078619186	40.0	35.0	41.0	23.0	41.0
42-43	34.87542107999201	40.0	35.0	41.0	21.5	41.0
44-45	34.74172525578961	40.0	35.0	41.0	20.5	41.0
46-47	34.57110835544812	39.5	35.0	41.0	18.5	41.0
48-49	34.41363377436455	39.0	35.0	41.0	15.0	41.0
50-51	32.72466510692453	37.0	32.0	39.5	12.5	40.0
52-53	32.8726484575565	37.5	32.5	39.5	11.0	40.0
54-55	33.724983417613544	39.0	34.0	40.5	10.0	41.0
56-57	33.74356369266481	39.0	34.0	41.0	8.0	41.0
58-59	33.57643783664912	38.0	34.0	41.0	7.5	41.0
60-61	33.3421202692778	38.0	33.5	40.0	6.0	41.0
62-63	33.06738435748957	37.0	33.0	40.0	2.0	41.0
64-65	32.7866414428662	37.0	33.0	40.0	2.0	41.0
66-67	32.44997335555779	36.0	33.0	39.5	2.0	41.0
68-69	32.09517608153021	36.0	32.0	39.0	2.0	41.0
70-71	31.72078847395217	35.0	32.0	39.0	2.0	41.0
72-73	31.37153525928095	35.0	32.0	37.5	2.0	40.0
74-75	30.980290818603805	35.0	31.5	37.0	2.0	39.5
76-77	30.6079846784084	35.0	31.0	37.0	2.0	39.0
78-79	30.26488132109013	35.0	31.0	36.0	2.0	39.0
80-81	29.91819118913642	35.0	31.0	36.0	2.0	37.0
82-83	29.61902270912627	35.0	31.0	35.0	2.0	37.0
84-85	29.37849778694049	35.0	30.5	35.0	2.0	36.5
86-87	29.15520639514034	35.0	30.0	35.0	2.0	36.0
88-89	28.956711820944605	35.0	30.0	35.0	2.0	36.0
90-91	28.709866623672003	34.0	30.0	35.0	2.0	36.0
92-93	28.527991779641276	34.0	30.0	35.0	2.0	35.0
94-95	28.321344124678205	34.0	29.5	35.0	2.0	35.0
96-97	28.080428279029846	34.0	29.0	35.0	2.0	35.0
98-99	27.863467831948554	34.0	29.0	35.0	2.0	35.0
100-101	26.841387917590133	33.0	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	66545.0
3	11021.0
4	7150.0
5	5529.0
6	4931.0
7	5006.0
8	5332.0
9	5588.0
10	6189.0
11	6619.0
12	6981.0
13	7065.0
14	7580.0
15	8414.0
16	8665.0
17	9745.0
18	8539.0
19	7554.0
20	7423.0
21	7862.0
22	8226.0
23	9276.0
24	10818.0
25	13013.0
26	15145.0
27	18065.0
28	21361.0
29	25143.0
30	29868.0
31	36062.0
32	44939.0
33	57975.0
34	80533.0
35	116602.0
36	192436.0
37	346957.0
38	381394.0
39	75464.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.56459189682709	18.849303662303942	13.175410202594149	24.41069423827482
2	15.637992614708661	19.501510627773488	39.16624259958459	25.694254157933255
3	19.13518123262739	23.92997758870077	29.369180341868685	27.56566083680316
4	13.33515212620841	15.553585196607555	36.720953680090155	34.39030899709389
5	14.131896115633715	37.33899648978396	34.35967171562921	14.169435678953116
6	33.55097273727053	35.40283447014498	17.013164486419026	14.033028306165463
7	28.9844461412961	31.844266960084987	20.88815165427227	18.28313524434664
8	26.68852801135478	34.747976956063674	19.8702666937809	18.693228338800647
9	26.758337172206204	15.56388744128303	19.40673258263359	38.27104280387717
10-11	25.580914831174223	25.322058518364805	27.87664897874485	21.220377671716122
12-13	26.11355560470236	22.71802850422488	27.818300834184672	23.350115056888086
14-15	23.210852024080157	24.39727697194631	25.05911665558214	27.33275434839139
16-17	23.363194545943447	27.5877699968189	25.83970763543607	23.20932782180158
18-19	23.3423720432613	26.81944428386826	27.327912478963285	22.510271193907155
20-21	23.699006923490597	26.031364117973858	27.444136852334637	22.825492106200905
22-23	23.546355179925936	25.78432846420325	27.14377677376618	23.525539582104635
24-25	23.361599082971672	26.043242772041904	27.211766000036175	23.383392144950253
26-27	23.182231082028178	26.317092376126432	27.148005535665792	23.352671006179595
28-29	23.25760843304742	26.540325102752565	27.00377512861672	23.198291335583292
30-31	23.59112763144634	26.205826382343	26.96432911827651	23.238716867934148
32-33	23.270031244470122	26.204094771434594	26.911622996238272	23.614250987857012
34-35	23.59361193882974	26.35053002151031	26.949658997709065	23.106199041950884
36-37	23.603870295429232	26.183118820772695	26.92769366740901	23.285317216389064
38-39	23.390685850104912	26.378670068554484	26.9368219281863	23.29382215315431
40-41	23.610884290027613	26.186369770300306	26.953013506219904	23.249732433452174
42-43	23.329024178399013	26.22475253053935	26.78676455735909	23.659458733702543
44-45	23.380491326518644	26.3308677817133	26.85885239149202	23.429788500276032
46-47	23.482410229102264	26.368247562664475	26.60105925011412	23.54828295811914
48-49	23.339519531666475	26.505350333944826	26.54140431750908	23.61372581687962
50-51	23.441680896874303	26.53355111738065	26.648362907417035	23.376405078328013
52-53	23.633061636034515	26.361637073984923	26.59747765513698	23.407823634843584
54-55	23.209467064790566	26.265099672539726	26.962222381936407	23.5632108807333
56-57	23.317189456601028	26.428860808219532	26.701938453276814	23.552011281902622
58-59	23.389396674573035	26.29820969162786	26.824390107713135	23.48800352608597
60-61	23.39228410426154	26.449670960007886	26.833065816315017	23.324979119415556
62-63	23.402864406981028	26.357058844495135	26.874933171757494	23.365143576766346
64-65	23.114484154799893	26.189964191772425	26.91085129487018	23.7847003585575
66-67	23.18982832083959	26.425113441533544	26.70555807829344	23.67950015933342
68-69	23.3556846064887	26.251727724310143	26.8589185024944	23.533669166706762
70-71	23.18064149457582	26.17529837889264	26.79022881505408	23.853831311477457
72-73	23.214570481595505	26.3353062634766	26.70173020162968	23.748393053298216
74-75	23.175081797410872	26.48834464846861	26.71367728731337	23.622896266807146
76-77	23.222589359396608	26.194143602800533	26.74725471173397	23.836012326068886
78-79	23.176204309315466	26.209626157675558	26.792726322230344	23.821443210778632
80-81	23.349269305855415	26.366420032390796	26.544652606889045	23.73965805486474
82-83	23.332577836699254	26.321255189831156	26.71306386362563	23.63310310984396
84-85	23.33756111470852	26.40309865681306	26.600952884495427	23.658387343982987
86-87	23.162296292225125	26.501893176987167	26.63281873183137	23.702991798956337
88-89	23.082437382466793	26.321882789127976	26.741957237965934	23.853722590439297
90-91	23.221705799432875	26.17898134490754	26.804481737794873	23.79483111786471
92-93	23.307538894423036	26.09930388586236	26.896102194582188	23.69705502513242
94-95	23.284474521157495	25.974769551844297	26.931236362080526	23.809519564917686
96-97	23.203111722262566	25.889976099329807	26.958494127043174	23.948418051364452
98-99	23.017632466317085	25.844858063845273	26.902924880048722	24.234584589788916
100-101	23.215571829941382	25.863408065566603	27.030148418595584	23.890871685896432
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	49.0
1	40.0
2	49.5
3	86.5
4	105.0
5	125.0
6	184.5
7	315.5
8	482.5
9	550.0
10	514.0
11	473.5
12	472.0
13	511.5
14	616.5
15	709.0
16	794.0
17	880.0
18	985.5
19	1115.5
20	1242.5
21	1412.0
22	1633.0
23	1934.0
24	2347.5
25	2904.0
26	3746.0
27	4967.0
28	6546.0
29	8603.0
30	11312.5
31	14737.5
32	19235.0
33	22828.5
34	25607.5
35	29268.0
36	34048.0
37	38620.5
38	42013.0
39	45900.0
40	49511.5
41	51924.5
42	54379.5
43	57632.5
44	61240.5
45	65116.5
46	67203.5
47	68361.0
48	70219.5
49	70987.0
50	70124.0
51	69476.0
52	68527.5
53	66799.5
54	64975.0
55	61752.5
56	56914.0
57	51056.0
58	45707.5
59	39609.5
60	32625.0
61	27134.0
62	22446.0
63	17161.0
64	12220.0
65	8850.5
66	6646.5
67	5028.5
68	3822.5
69	2888.5
70	2216.5
71	1678.5
72	1194.0
73	893.0
74	705.0
75	541.0
76	407.0
77	279.0
78	171.0
79	128.0
80	109.5
81	84.0
82	61.0
83	48.0
84	38.5
85	30.0
86	25.5
87	21.0
88	21.5
89	20.0
90	16.0
91	13.5
92	12.0
93	7.5
94	5.5
95	6.5
96	5.5
97	4.0
98	5.0
99	2.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05050292496107066
2	0.057912391651368585
3	0.049080307356533466
4	0.05518570790933895
5	0.048013344153130556
6	0.01754561712262549
7	0.014641106180028701
8	0.020153749397610356
9	0.02003519793056559
10-11	0.02258405447202808
12-13	0.028096697689609734
14-15	0.04424933507445921
16-17	0.03002315902908719
18-19	0.02350282834162502
20-21	0.02691118301916207
22-23	0.047153846017056
24-25	0.04235251160174294
26-27	0.030408451296982688
28-29	0.019768457129714867
30-31	0.013099937108446733
32-33	0.010225064032611138
34-35	0.009810133897954454
36-37	0.0016597205386267354
38-39	0.007379828823536733
40-41	0.00400111201276088
42-43	0.22483285725040025
44-45	0.19943320543605894
46-47	0.2063684662581778
48-49	0.20417526411784964
50-51	0.20189314837723787
52-53	0.20619063905761068
54-55	0.20556824385562564
56-57	0.21499308548568463
58-59	0.21208857454308783
60-61	0.21629715162317706
62-63	0.21404467374932645
64-65	0.21451887961750551
66-67	0.2047680214530735
68-69	0.20497548652040182
70-71	0.188615384068224
72-73	0.197121451828686
74-75	0.19549136915682047
76-77	0.19457259528722354
78-79	0.219379489766341
80-81	0.22744098952538513
82-83	0.22592945832056438
84-85	0.22056500443678864
86-87	0.23790315649208582
88-89	0.23686583115544407
90-91	0.23582850581880238
92-93	0.23979997996480207
94-95	0.2503214226650251
96-97	0.25091418000024895
98-99	0.24042237516678708
100-101	0.2503214226650251
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1687031.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.96477016830833
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.18665862893238	55.03610705987671
2	10.421645722394413	13.957662211513512
3	3.0777457655011657	6.183016134698092
4	1.4118193428180357	3.7816863124392746
5	0.7702415450293926	2.5789524018487993
6	0.495536535960892	1.9910094144372477
7	0.3293596726844068	1.5438846348814395
8	0.23398841862165026	1.2535184540035782
9	0.18332006893670336	1.1048387655226302
>10	0.8525244421129227	9.689435974680345
>50	0.02703550773417908	1.2156157476483722
>100	0.009147607045227659	1.1524903489464213
>500	7.991527326269343E-4	0.3455884883925761
>1k	1.775894961393187E-4	0.1661940511110203
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.927573352238341E-5	0.0
5	0.0	0.0	0.0	5.927573352238341E-5	0.0
6	0.0	0.0	0.0	5.927573352238341E-5	0.0
7	0.0	0.0	0.0	5.927573352238341E-5	0.0
8	0.0	0.0	0.0	5.927573352238341E-5	0.0
9	0.0	0.0	0.0	1.1855146704476682E-4	0.0
10-11	0.0	0.0	0.0	1.1855146704476682E-4	5.927573352238341E-5
12-13	0.0	0.0	0.0	1.1855146704476682E-4	1.778272005671502E-4
14-15	0.0	0.0	0.0	1.4818933380595853E-4	1.778272005671502E-4
16-17	0.0	0.0	0.0	1.778272005671502E-4	2.3710293408953363E-4
18-19	0.0	0.0	0.0	1.778272005671502E-4	2.3710293408953363E-4
20-21	0.0	0.0	0.0	1.778272005671502E-4	2.3710293408953363E-4
22-23	0.0	0.0	0.0	2.9637866761191706E-4	3.556544011343004E-4
24-25	0.0	0.0	0.0	5.927573352238341E-4	4.7420586817906727E-4
26-27	0.0	0.0	0.0	9.780496031193261E-4	4.7420586817906727E-4
28-29	0.0	0.0	0.0	0.003111976009925129	4.7420586817906727E-4
30-31	0.0	0.0	0.0	0.010462166966700671	4.7420586817906727E-4
32-33	0.0	0.0	0.0	0.02439196434446077	4.7420586817906727E-4
34-35	0.0	0.0	0.0	0.04395295640684729	5.334816017014506E-4
36-37	0.0	0.0	0.0	0.07611004184274028	5.334816017014506E-4
38-39	0.0	0.0	0.0	0.12951747774640776	5.334816017014506E-4
40-41	0.0	0.0	0.0	0.18879321126879114	5.334816017014506E-4
42-43	0.0	0.0	0.0	0.24993613039712964	5.334816017014506E-4
44-45	0.0	0.0	0.0	0.32788371997906385	5.334816017014506E-4
46-47	0.0	0.0	0.0	0.41679732026263894	5.334816017014506E-4
48-49	0.0	0.0	0.0	0.5079633984200645	6.520330687462175E-4
50-51	0.0	0.0	0.0	0.6080208366058477	7.113088022686009E-4
52-53	0.0	0.0	0.0	0.7098269089305413	7.705845357909842E-4
54-55	0.0	0.0	0.0	0.8253256756989054	8.594981360745594E-4
56-57	0.0	0.0	0.0	0.9562954089166115	8.891360028357511E-4
58-59	0.0	0.0	0.0	1.099713046174018	8.891360028357511E-4
60-61	0.0	0.0	0.0	1.2524369735944392	9.484117363581345E-4
62-63	0.0	0.0	0.0	1.4097251324960833	0.001007687469880518
64-65	0.0	0.0	0.0	1.5865742834601142	0.001007687469880518
66-67	0.0	0.0	0.0	1.7897418601080834	0.0010373253366417097
68-69	0.0	0.0	0.0	2.0133299269545137	0.0010669632034029013
70-71	0.0	0.0	0.0	2.2444756498250475	0.0011855146704476682
72-73	0.0	0.0	0.0	2.4841867161895665	0.001304066137492435
74-75	0.0	0.0	0.0	2.736612427394636	0.0013633418710148183
76-77	0.0	0.0	0.0	3.0195058656302107	0.0014226176045372017
78-79	0.0	0.0	0.0	3.328806643150007	0.0014226176045372017
80-81	0.0	0.0	0.0	3.6518001151134745	0.0014522554712983935
82-83	0.0	0.0	0.0	3.9931097887353584	0.001481893338059585
84-85	0.0	0.0	0.0	4.3607378880411805	0.001481893338059585
86-87	0.0	0.0	0.0	4.7585966114434175	0.0015708069383431602
88-89	0.0	2.9637866761191704E-5	0.0	5.177261117312011	0.001600444805104352
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1535	0.0	56.273132	1
GTATCAA	2795	0.0	44.489502	1
TCAACGC	3520	0.0	34.377254	4
ATCAACG	3655	0.0	33.108486	3
TATCAAC	3705	0.0	32.921753	2
CAACGCA	3740	0.0	32.35314	5
AACGCAG	3890	0.0	31.338406	6
GTACATG	4385	0.0	27.383503	1
ACGCAGA	4580	0.0	26.10469	7
TACATGG	4625	0.0	25.859901	2
CGCAGAG	4705	0.0	25.110876	8
ACATGGG	4810	0.0	25.05966	3
CATGGGG	3935	0.0	22.43065	4
GCAGAGT	5470	0.0	20.55811	9
GAGTACT	2805	0.0	19.36902	12-13
GTATTAG	470	0.0	19.186415	1
TAAGGTG	745	0.0	19.107897	5
TATACCG	150	7.7765854E-4	18.980513	5
GAGTACA	3340	0.0	18.899193	1
CAGAGTA	4860	0.0	18.842981	10-11
>>END_MODULE