FastQCFastQC Report
Mon 15 Aug 2016
SRR2921397_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921397_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1687031
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18000.10669632034029013No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15650.051.6187741
GTATCAA28550.043.1088831
TCAACGC37050.032.9203154
ATCAACG38100.031.7639373
CAACGCA38400.031.6901075
TATCAAC38650.031.1891352
AACGCAG40650.030.0520866
ACGCAGA47100.025.633157
GTACATG43700.025.2278161
CGCAGAG48400.024.6500348
TACATGG44850.023.5972562
ACATGGG45900.022.747263
GTACCGT2257.1031536E-821.126116
GCAGAGT55000.020.1364279
CATGGGG38500.019.4767044
CGTATAC1507.768018E-418.9836243
GAGTACT29700.018.6904512-13
AGAGTAC46750.018.45373210-11
GTACTTT32050.017.6903614-15
ATACCGT2152.3809196E-517.6869776
Produced by FastQC (version 0.11.5)