FastQCFastQC Report
Mon 15 Aug 2016
SRR2921396_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921396_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1421380
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11650.054.1784861
GTATCAA21000.040.4515231
TCAACGC25850.031.02724
TATCAAC26750.030.5166172
CAACGCA26450.030.3212345
ATCAACG26700.029.8627473
AACGCAG27400.029.2617136
GTACATG36050.025.5386281
TACATGG36950.024.6614442
ACATGGG38850.023.4553493
ACGCAGA33900.023.0945227
CGCAGAG34300.022.5501168
CGTTATA1503.0534444E-522.1482032
GGACCGT1605.0374765E-520.7559056
AATTCGG1150.003745540720.6313428
GAGTACA28650.020.374251
TGGACCG3503.6379788E-1220.338045
CATGGGG34450.020.1131484
GTCTTAG5250.019.8867821
CGTATAC1507.7656796E-418.9841733
Produced by FastQC (version 0.11.5)