##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921396_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1421380
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.227775119953847	31.0	31.0	34.0	26.0	34.0
2	30.289323052245003	31.0	31.0	34.0	26.0	34.0
3	30.340163080949498	33.0	31.0	34.0	26.0	34.0
4	33.7772270610252	37.0	35.0	37.0	28.0	37.0
5	33.773093050415795	37.0	35.0	37.0	28.0	37.0
6	33.82268007147983	37.0	35.0	37.0	29.0	37.0
7	33.73099241582124	37.0	35.0	37.0	29.0	37.0
8	33.69040087801995	37.0	35.0	37.0	28.0	37.0
9	35.23789064148926	39.0	35.0	39.0	29.0	39.0
10-11	35.13392618441233	39.0	35.0	39.0	28.0	39.0
12-13	35.01668273086719	39.0	35.0	39.0	27.0	39.0
14-15	36.147121811197565	40.0	36.0	41.0	27.0	41.0
16-17	36.09953003419213	40.0	36.0	41.0	27.0	41.0
18-19	36.04608619792033	40.0	36.5	41.0	27.0	41.0
20-21	35.98745409390874	40.0	36.0	41.0	27.0	41.0
22-23	35.87737586007964	40.0	36.0	41.0	27.0	41.0
24-25	35.783008062587065	40.0	36.0	41.0	27.0	41.0
26-27	35.63518587569827	40.0	36.0	41.0	26.0	41.0
28-29	35.49660259747569	40.0	36.0	41.0	25.0	41.0
30-31	35.34603835708959	40.0	35.5	41.0	25.0	41.0
32-33	35.166929673978814	39.0	35.0	41.0	24.0	41.0
34-35	35.00252536267571	39.0	35.0	41.0	24.0	41.0
36-37	34.83561644317494	39.0	35.0	41.0	23.5	41.0
38-39	35.055542500949784	39.0	35.0	41.0	24.0	41.0
40-41	35.17386624266558	40.0	35.0	41.0	24.0	41.0
42-43	35.008925832641516	40.0	35.0	41.0	22.5	41.0
44-45	34.88603047742335	40.0	35.0	41.0	22.0	41.0
46-47	34.723792370794584	40.0	35.0	41.0	20.5	41.0
48-49	34.576742320843124	39.0	35.0	41.0	18.5	41.0
50-51	32.87519241863541	37.0	32.5	39.5	15.0	40.0
52-53	33.026930869999575	37.5	32.5	39.5	13.5	40.0
54-55	33.88512361226414	39.0	34.0	40.5	13.5	41.0
56-57	33.90351981876768	39.0	34.0	41.0	13.0	41.0
58-59	33.73168575609619	38.0	34.0	41.0	11.0	41.0
60-61	33.494050851988916	38.0	34.0	40.0	8.0	41.0
62-63	33.212853705553755	37.0	33.5	40.0	7.0	41.0
64-65	32.93089391999325	37.0	33.0	40.0	4.0	41.0
66-67	32.59432980624464	36.0	33.0	39.5	2.0	41.0
68-69	32.23497903445947	36.0	33.0	39.0	2.0	41.0
70-71	31.85569130000422	35.0	32.0	39.0	2.0	40.5
72-73	31.50917770054454	35.0	32.0	37.5	2.0	40.0
74-75	31.125122064472556	35.0	32.0	37.0	2.0	39.0
76-77	30.75182322813041	35.0	31.5	37.0	2.0	39.0
78-79	30.41437792849203	35.0	31.0	36.0	2.0	39.0
80-81	30.07146013029591	35.0	31.0	36.0	2.0	37.0
82-83	29.771082328441373	35.0	31.0	35.0	2.0	37.0
84-85	29.541112158606424	35.0	31.0	35.0	2.0	36.5
86-87	29.317649748835642	35.0	31.0	35.0	2.0	36.0
88-89	29.124384752845828	35.0	30.0	35.0	2.0	36.0
90-91	28.881709676511562	34.5	30.0	35.0	2.0	36.0
92-93	28.69854226174563	34.0	30.0	35.0	2.0	35.0
94-95	28.500517454867804	34.0	30.0	35.0	2.0	35.0
96-97	28.261517328230312	34.0	30.0	35.0	2.0	35.0
98-99	28.051981524996833	34.0	29.0	35.0	2.0	35.0
100-101	27.034457358341893	33.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	55377.0
3	8983.0
4	6058.0
5	4481.0
6	4123.0
7	4159.0
8	4313.0
9	4705.0
10	5034.0
11	5496.0
12	5520.0
13	5592.0
14	6136.0
15	6552.0
16	6783.0
17	7202.0
18	6663.0
19	6027.0
20	5936.0
21	6229.0
22	6659.0
23	7707.0
24	8781.0
25	10377.0
26	12597.0
27	14769.0
28	17614.0
29	20848.0
30	24628.0
31	30470.0
32	37832.0
33	48444.0
34	68061.0
35	98513.0
36	164464.0
37	298051.0
38	323187.0
39	62994.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.8997396227451	18.88984542784157	13.09516686599124	25.115248083422088
2	16.03557547927568	19.498581162369792	39.2082104624404	25.257632895914128
3	19.765753298339693	23.013517156716098	29.009480944842426	28.211248600101786
4	13.336008362987444	15.05358858747875	36.265447416308525	35.344955633225275
5	14.352292764304039	36.970503250238444	34.30604681610372	14.371157169353788
6	34.28241709912921	34.85421760928842	16.616624153399595	14.246741138182777
7	29.603823915798422	31.349087119615582	20.343015323387007	18.704073641198992
8	27.022822014743674	34.81067973462512	19.358250462323127	18.80824778830808
9	26.779069440144116	15.58237396909393	18.85636276634672	38.78219382441523
10-11	25.977422960770784	25.077522148305597	27.59728664564224	21.34776824528138
12-13	26.307363063707847	22.304325704043222	27.670550630519553	23.71776060172938
14-15	23.190221055995313	24.125939836393506	24.703531856248144	27.980307251363033
16-17	23.672469760281505	27.442269628326372	25.53996921046844	23.34529140092369
18-19	23.63492997313209	26.68431119486906	27.015022948207847	22.665735883791008
20-21	23.87867852102281	25.834738771478747	27.15802637426516	23.128556333233284
22-23	23.644938260307985	25.69800078483405	26.856493269960037	23.800567684897928
24-25	23.54177884293914	25.8992225964364	26.86715865860053	23.691839902023922
26-27	23.412743934065578	26.179951538678626	26.778750773262804	23.628553753992993
28-29	23.489303100630092	26.403418278953968	26.653125189118594	23.45415343129735
30-31	23.80902455886052	26.083124732835977	26.592772542486777	23.515078165816725
32-33	23.47727151992632	26.13230783194902	26.623785773314328	23.76663487481033
34-35	23.73456531696818	26.205134844370832	26.69752489215335	23.36277494650764
36-37	23.809252438694063	26.15880620956763	26.599231015474672	23.432710336263636
38-39	23.70024126088893	26.17828398707365	26.64356781865399	23.47790693338343
40-41	23.734084370688436	26.03651668199276	26.66241241434917	23.566986532969636
42-43	23.585555827779668	26.068075220809988	26.52790287357031	23.818466077840036
44-45	23.548038870509522	26.2046062251601	26.69434673247291	23.553008171857474
46-47	23.760596520858915	26.12496175877222	26.38154806129311	23.732893659075753
48-49	23.549615199716918	26.311626903735785	26.37016814482419	23.768589751723106
50-51	23.61635268891179	26.333600718415312	26.48659526686112	23.563451325811776
52-53	23.81115350386381	26.192318198239477	26.454193798517917	23.542334499378796
54-55	23.319583036099463	26.089250519513108	26.766415063483517	23.824751380903912
56-57	23.501863780963877	26.27323117052494	26.472351712651683	23.752553335859496
58-59	23.518997166453833	26.246871344173815	26.585881756636486	23.648249732735867
60-61	23.538306984613758	26.23669786214068	26.639560090942737	23.585435062302825
62-63	23.587142264971366	26.164498191057085	26.706335107027947	23.542024436943606
64-65	23.292724365662593	26.016822137374447	26.780346397008646	23.910107099954317
66-67	23.301826343358755	26.246997977622023	26.641463894314715	23.809711784704508
68-69	23.508244122745122	26.069416845769243	26.66660557559421	23.755733455891423
70-71	23.397106323477487	25.932614788915537	26.69726341843995	23.97301546916703
72-73	23.369499522298998	26.16941592902889	26.522008959140443	23.93907558953167
74-75	23.31679440023372	26.22194451757099	26.62401715284364	23.837243929351654
76-77	23.344952278451526	25.925401227160773	26.681989436874098	24.047657057513604
78-79	23.31001624347159	25.92712575154257	26.729640669591998	24.033217335393847
80-81	23.44505417412573	26.138883463759704	26.47153593358796	23.944526428526604
82-83	23.402739440140053	26.279666280998843	26.552665514598623	23.764928764262482
84-85	23.391195254107515	26.3127152284731	26.518967953804466	23.77712156361492
86-87	23.36769432327959	26.385263344939773	26.469843700861244	23.77719863091939
88-89	23.259582257434456	26.20279736058678	26.560435314209478	23.97718506776929
90-91	23.351005994931594	26.030920929235553	26.621282782799017	23.996790293033836
92-93	23.412591801261506	26.000162183697718	26.72202066784426	23.865225347196514
94-95	23.367487841756653	25.826323302077338	26.769257134152536	24.03693172201347
96-97	23.32769973049681	25.702416713358687	26.83032441583253	24.139559140311974
98-99	23.20721774014415	25.718928692061375	26.813196654436833	24.260656913357643
100-101	23.344500639466677	25.65659493426734	26.9037224471316	24.09518197913438
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	28.0
1	28.5
2	48.5
3	84.0
4	80.0
5	91.0
6	153.5
7	275.0
8	412.5
9	443.5
10	374.0
11	326.5
12	345.5
13	375.0
14	438.5
15	525.5
16	587.5
17	609.5
18	658.5
19	760.0
20	865.0
21	978.0
22	1108.0
23	1318.5
24	1610.0
25	1993.0
26	2591.5
27	3412.5
28	4384.0
29	5840.5
30	7980.5
31	10867.0
32	14853.5
33	17612.0
34	19618.5
35	22669.0
36	26641.0
37	30903.5
38	34597.0
39	37607.5
40	39876.0
41	42316.5
42	44930.0
43	48374.5
44	51808.0
45	55311.0
46	57413.0
47	58708.5
48	60697.0
49	61160.5
50	60656.5
51	59809.5
52	58886.0
53	57910.0
54	56904.0
55	54254.5
56	49516.0
57	44655.0
58	40404.5
59	35505.5
60	29346.5
61	23729.0
62	19268.5
63	14651.0
64	10329.0
65	7579.5
66	5686.5
67	4300.5
68	3378.5
69	2570.0
70	1918.5
71	1371.5
72	989.5
73	766.5
74	578.5
75	436.5
76	319.0
77	225.5
78	144.5
79	102.5
80	80.5
81	68.0
82	53.5
83	41.0
84	45.5
85	39.0
86	21.0
87	18.0
88	21.5
89	19.0
90	19.5
91	20.5
92	15.0
93	9.0
94	6.0
95	5.5
96	7.0
97	5.5
98	6.5
99	4.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05269526797900631
2	0.059871392590299576
3	0.05213243467615979
4	0.05874572598460651
5	0.050303226441908565
6	0.017940311528233124
7	0.015407561665423742
8	0.02082483220532159
9	0.021106248856744853
10-11	0.023533467475270513
12-13	0.028774852608028815
14-15	0.04601162250770378
16-17	0.03113171706369866
18-19	0.024905373650958927
20-21	0.028950738015168356
22-23	0.04907202859193179
24-25	0.04397135178488511
26-27	0.03187043577368473
28-29	0.02209120713672628
30-31	0.014246717978302776
32-33	0.011010426486935232
34-35	0.010482770265516611
36-37	0.0017588540713954044
38-39	0.006824353797014169
40-41	0.004010187282781522
42-43	0.2126454572317044
44-45	0.1878104377436013
46-47	0.19375536450491776
48-49	0.19150403129353163
50-51	0.1900969480364153
52-53	0.19470514570347128
54-55	0.1930518228763596
56-57	0.2034994160604483
58-59	0.19977064542909007
60-61	0.204378843096146
62-63	0.20205715572190405
64-65	0.20346423897902038
66-67	0.19368501034206195
68-69	0.19227792708494562
70-71	0.17539292799954972
72-73	0.18330777132082907
74-75	0.18337812548368487
76-77	0.1812675005980104
78-79	0.20852973870463917
80-81	0.21690188408448127
82-83	0.21535409250165333
84-85	0.20926845741462524
86-87	0.2252740294643234
88-89	0.22414836285863035
90-91	0.22386694620720707
92-93	0.22759571683856533
94-95	0.2400484036640448
96-97	0.23923933079120294
98-99	0.22956563339852817
100-101	0.23930968495405872
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1421380.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.37357919668433
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.22996943313574	54.57897388511167
2	10.23882745627039	13.591752500998973
3	3.1310558593937285	6.234581521581365
4	1.4486400238330384	3.8460569339747543
5	0.7837741990682687	2.601094943708779
6	0.4992194616692516	1.988098948537811
7	0.328845246465895	1.5278645206830084
8	0.25234529015992735	1.3399248081072181
9	0.18249360428008363	1.0901478326915253
>10	0.8581483280445732	9.651315870955795
>50	0.032749443956714336	1.4429134074923071
>100	0.012655517120083937	1.5308793912469925
>500	0.001169791885520017	0.4956464295784266
>1k	1.0634471686545609E-4	0.0807490053314896
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	7.035416285581618E-5	0.0	0.0	0.0	0.0
7	7.035416285581618E-5	0.0	0.0	0.0	0.0
8	7.035416285581618E-5	0.0	0.0	0.0	0.0
9	7.035416285581618E-5	0.0	0.0	0.0	0.0
10-11	7.035416285581618E-5	0.0	0.0	0.0	0.0
12-13	7.035416285581618E-5	0.0	0.0	0.0	0.0
14-15	7.035416285581618E-5	0.0	0.0	0.0	0.0
16-17	7.035416285581618E-5	0.0	0.0	3.517708142790809E-5	0.0
18-19	7.035416285581618E-5	0.0	0.0	7.035416285581618E-5	0.0
20-21	7.035416285581618E-5	0.0	0.0	1.4070832571163236E-4	0.0
22-23	7.035416285581618E-5	0.0	0.0	2.814166514232647E-4	3.517708142790809E-5
24-25	7.035416285581618E-5	0.0	0.0	5.276562214186213E-4	7.035416285581618E-5
26-27	7.035416285581618E-5	3.517708142790809E-5	0.0	8.090728728418861E-4	7.035416285581618E-5
28-29	7.035416285581618E-5	7.035416285581618E-5	0.0	0.0023216873742419337	7.035416285581618E-5
30-31	7.035416285581618E-5	1.4070832571163236E-4	0.0	0.00904050992697238	7.035416285581618E-5
32-33	7.035416285581618E-5	1.4070832571163236E-4	0.0	0.02138766550816812	1.4070832571163236E-4
34-35	7.035416285581618E-5	1.4070832571163236E-4	0.0	0.041157185270652465	1.4070832571163236E-4
36-37	7.035416285581618E-5	1.4070832571163236E-4	0.0	0.07411811056860235	1.4070832571163236E-4
38-39	7.035416285581618E-5	1.4070832571163236E-4	0.0	0.12727068060617147	1.4070832571163236E-4
40-41	7.035416285581618E-5	1.4070832571163236E-4	0.0	0.18489073998508493	1.4070832571163236E-4
42-43	7.035416285581618E-5	1.4070832571163236E-4	0.0	0.24852608028817066	1.7588540713954045E-4
44-45	7.035416285581618E-5	1.4070832571163236E-4	0.0	0.32116675343680084	2.1106248856744855E-4
46-47	7.035416285581618E-5	1.4070832571163236E-4	0.0	0.40830038413372916	2.462395699953566E-4
48-49	7.035416285581618E-5	1.4070832571163236E-4	0.0	0.5008512853705553	2.814166514232647E-4
50-51	7.035416285581618E-5	1.4070832571163236E-4	0.0	0.602055748638647	2.814166514232647E-4
52-53	7.035416285581618E-5	1.4070832571163236E-4	0.0	0.7040692847795804	2.814166514232647E-4
54-55	7.035416285581618E-5	1.4070832571163236E-4	0.0	0.8157916953946165	2.814166514232647E-4
56-57	7.035416285581618E-5	1.4070832571163236E-4	0.0	0.9474595111792765	2.814166514232647E-4
58-59	7.035416285581618E-5	1.4070832571163236E-4	0.0	1.08848443062376	2.814166514232647E-4
60-61	7.035416285581618E-5	1.4070832571163236E-4	0.0	1.2403087140666114	2.814166514232647E-4
62-63	7.035416285581618E-5	1.4070832571163236E-4	0.0	1.3983593409222024	3.517708142790809E-4
64-65	7.035416285581618E-5	1.4070832571163236E-4	0.0	1.5672796859390172	3.517708142790809E-4
66-67	7.035416285581618E-5	1.4070832571163236E-4	0.0	1.76750763342667	3.517708142790809E-4
68-69	7.035416285581618E-5	1.4070832571163236E-4	0.0	1.986731204885393	3.517708142790809E-4
70-71	7.035416285581618E-5	1.4070832571163236E-4	0.0	2.2200607859967074	4.221249771348971E-4
72-73	7.035416285581618E-5	1.4070832571163236E-4	0.0	2.4661244705849246	4.221249771348971E-4
74-75	7.035416285581618E-5	1.4070832571163236E-4	0.0	2.72129901926297	4.221249771348971E-4
76-77	7.035416285581618E-5	1.4070832571163236E-4	0.0	3.005178066386188	4.221249771348971E-4
78-79	7.035416285581618E-5	1.4070832571163236E-4	0.0	3.323178882494477	4.221249771348971E-4
80-81	7.035416285581618E-5	1.4070832571163236E-4	0.0	3.668301228383683	4.5730205856280514E-4
82-83	7.035416285581618E-5	1.4070832571163236E-4	0.0	4.019438855197063	5.628333028465294E-4
84-85	7.035416285581618E-5	1.4070832571163236E-4	0.0	4.387637366502976	5.628333028465294E-4
86-87	7.035416285581618E-5	1.4070832571163236E-4	0.0	4.786369584488314	6.331874657023456E-4
88-89	7.035416285581618E-5	1.4070832571163236E-4	0.0	5.214931967524518	6.331874657023456E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1165	0.0	54.178486	1
GTATCAA	2100	0.0	40.451523	1
TCAACGC	2585	0.0	31.0272	4
TATCAAC	2675	0.0	30.516617	2
CAACGCA	2645	0.0	30.321234	5
ATCAACG	2670	0.0	29.862747	3
AACGCAG	2740	0.0	29.261713	6
GTACATG	3605	0.0	25.538628	1
TACATGG	3695	0.0	24.661444	2
ACATGGG	3885	0.0	23.455349	3
ACGCAGA	3390	0.0	23.094522	7
CGCAGAG	3430	0.0	22.550116	8
CGTTATA	150	3.0534444E-5	22.148203	2
GGACCGT	160	5.0374765E-5	20.755905	6
AATTCGG	115	0.0037455407	20.631342	8
GAGTACA	2865	0.0	20.37425	1
TGGACCG	350	3.6379788E-12	20.33804	5
CATGGGG	3445	0.0	20.113148	4
GTCTTAG	525	0.0	19.886782	1
CGTATAC	150	7.7656796E-4	18.984173	3
>>END_MODULE