FastQCFastQC Report
Mon 15 Aug 2016
SRR2921396_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921396_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1421380
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12650.049.9688071
GTATCAA22100.041.290191
TCAACGC28900.031.2052614
CAACGCA29150.030.5006685
TATCAAC29750.030.3136842
ATCAACG30200.029.8619883
AACGCAG30750.029.0641576
GTACATG39150.025.3718681
TACATGG40800.023.6160872
ACATGGG40800.023.034413
ACGCAGA39000.022.3065057
CGCAGAG39350.021.987298
TATACTG6200.020.7051495
CATGGGG35450.020.4855334
GTATTAC2601.4812031E-820.1074621
GTGGTAT6950.019.8312951
TACACTG14650.019.7969595
CTACACG2902.4629117E-919.6406254
GAGTACA29100.019.435331
CGTATAC1250.006057678718.9859373
Produced by FastQC (version 0.11.5)