##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921396_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1421380
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.362390775162165	33.0	31.0	34.0	29.0	34.0
2	31.49365124034389	34.0	31.0	34.0	28.0	34.0
3	31.567823523617893	34.0	31.0	34.0	28.0	34.0
4	35.05289999859291	37.0	35.0	37.0	32.0	37.0
5	34.79469388903741	37.0	35.0	37.0	32.0	37.0
6	34.756115183835426	37.0	35.0	37.0	32.0	37.0
7	34.62580027860248	37.0	35.0	37.0	32.0	37.0
8	34.58090095540953	37.0	35.0	37.0	32.0	37.0
9	36.20308854774937	39.0	37.0	39.0	32.0	39.0
10-11	36.05219716050598	39.0	37.0	39.0	32.0	39.0
12-13	35.98447529865342	39.0	37.0	39.0	31.0	39.0
14-15	37.23087668322334	40.0	38.0	41.0	31.0	41.0
16-17	37.215501132702016	40.0	38.0	41.0	31.0	41.0
18-19	37.1805453151163	40.0	38.0	41.0	31.0	41.0
20-21	37.14407829011243	40.0	38.0	41.0	31.0	41.0
22-23	37.03679487540278	40.0	38.0	41.0	31.0	41.0
24-25	36.977507422364184	40.0	37.5	41.0	30.5	41.0
26-27	36.836977444455385	40.0	37.0	41.0	30.0	41.0
28-29	36.68616203970789	40.0	37.0	41.0	30.0	41.0
30-31	36.54599719990432	40.0	37.0	41.0	30.0	41.0
32-33	36.49021619834245	40.0	37.0	41.0	30.0	41.0
34-35	36.616367544217596	40.0	37.0	41.0	30.0	41.0
36-37	36.59305885829264	40.0	37.0	41.0	30.0	41.0
38-39	36.50011151134812	40.0	37.0	41.0	30.0	41.0
40-41	36.374324248265765	40.0	37.0	41.0	29.5	41.0
42-43	36.26310733231085	40.0	36.5	41.0	29.0	41.0
44-45	36.1205680395109	40.0	36.0	41.0	28.5	41.0
46-47	35.9524511390339	40.0	36.0	41.0	28.0	41.0
48-49	35.78696970549747	40.0	35.5	41.0	27.0	41.0
50-51	35.63553201817952	40.0	35.0	41.0	27.0	41.0
52-53	35.46448627390283	39.0	35.0	41.0	26.5	41.0
54-55	35.25814841914196	39.0	35.0	41.0	26.0	41.0
56-57	34.998455374354506	39.0	35.0	41.0	26.0	41.0
58-59	34.775871688077785	38.5	35.0	40.5	25.5	41.0
60-61	34.503401272003266	38.0	34.5	40.0	24.5	41.0
62-63	34.209632188436586	37.5	34.0	40.0	24.0	41.0
64-65	33.89551562565957	37.0	34.0	40.0	23.0	41.0
66-67	33.56464034951948	36.5	34.0	39.5	22.5	41.0
68-69	33.207579605735276	36.0	34.0	39.0	22.0	41.0
70-71	32.82911431144381	35.5	33.0	39.0	21.5	40.0
72-73	32.43292961769548	35.0	33.0	37.5	20.0	40.0
74-75	32.028178601077826	35.0	33.0	37.0	19.0	39.0
76-77	29.2902541895904	33.0	29.0	34.5	11.5	36.5
78-79	30.932665437813956	34.5	31.5	36.0	14.5	38.0
80-81	31.130880552702305	35.0	32.0	36.0	17.0	37.0
82-83	30.96515393490833	35.0	32.0	35.0	14.0	37.0
84-85	30.726324768886574	35.0	32.0	35.0	9.5	36.5
86-87	30.480919599262688	35.0	32.0	35.0	7.0	36.0
88-89	30.202014239682562	35.0	32.0	35.0	2.0	36.0
90-91	30.013760922483783	34.5	31.0	35.0	2.0	35.5
92-93	29.87186290787826	34.0	31.0	35.0	2.0	35.0
94-95	29.775586753718216	34.0	31.0	35.0	2.0	35.0
96-97	29.58529809058802	34.0	31.0	35.0	2.0	35.0
98-99	29.322356794101506	34.0	31.0	35.0	2.0	35.0
100-101	28.24682597194276	33.0	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	28316.0
3	9364.0
4	4999.0
5	3434.0
6	3048.0
7	2984.0
8	3168.0
9	3362.0
10	3694.0
11	4123.0
12	4349.0
13	4793.0
14	5276.0
15	5793.0
16	6683.0
17	7319.0
18	6613.0
19	5637.0
20	5355.0
21	5159.0
22	5489.0
23	6095.0
24	7127.0
25	8585.0
26	10435.0
27	12341.0
28	14761.0
29	17491.0
30	21626.0
31	26634.0
32	34267.0
33	45121.0
34	65007.0
35	97415.0
36	171109.0
37	338527.0
38	364796.0
39	51082.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.98510581781313	20.241474720615184	13.443022830606244	25.330396630965442
2	15.58075348484454	21.129277478262154	37.23354034033386	26.056428696559443
3	18.34485932057067	24.37350530964672	28.823927147411382	28.457708222371224
4	13.076435989080574	16.506135141980693	35.27122674696761	35.14620212197113
5	14.076046146494642	37.60483281290796	34.18930816149444	14.129812879102968
6	34.216430590117504	35.69902368223133	16.192508447889033	13.892037279762143
7	29.49323896494956	31.712490678073422	20.36070579296177	18.433564564015253
8	27.132246528483776	35.510742229410994	19.017021105946235	18.339990136159
9	26.573892977247464	15.746316959574497	18.903178601077826	38.77661146210021
10-11	25.905117986299548	25.625918604348175	27.366046622669906	21.10291678668238
12-13	26.259859706615796	22.703558552489714	27.71914729257548	23.31743444831901
14-15	23.16222099643427	24.518190286693663	24.636074657991088	27.683514058880977
16-17	23.547395555580568	27.859865567076458	25.54411986114883	23.048619016194145
18-19	23.563177755053292	26.941070069851403	27.12912747484917	22.366624700246135
20-21	23.81321407753995	25.999519477856055	27.30716586562054	22.880100578983452
22-23	23.60266516483505	25.82072787943791	27.02575779588152	23.550849159845516
24-25	23.49684139874782	26.185821014706367	26.98238373610571	23.334953850440108
26-27	23.421298786457374	26.3780623069376	26.962055730019923	23.238583176585102
28-29	23.421547181732535	26.688881368286776	26.781051741351643	23.108519708629046
30-31	23.791098159209177	26.29377995581764	26.71945469725164	23.19566718772154
32-33	23.446492465635345	26.301142380669244	26.729561518623935	23.522803635071472
34-35	23.732187367521156	26.382731707145457	26.84613720278564	23.038943722547746
36-37	23.690594285794692	26.400831592055553	26.80734080499799	23.101233317151763
38-39	23.698593369591638	26.376316462468925	26.74198338159058	23.183106786348855
40-41	23.673392999177867	26.340801707164086	26.717427546313964	23.268377747344076
42-43	23.52548400022656	26.2536723718377	26.674251515122734	23.546592112813013
44-45	23.505545245864152	26.39561657329311	26.74016939165003	23.35866878919271
46-47	23.764792550725105	26.376211179119064	26.45353807487692	23.405458195278907
48-49	23.47942920127793	26.537686598276178	26.47541534398887	23.50746885645702
50-51	23.62048883005351	26.512963737558408	26.498188443398135	23.368358988989943
52-53	23.89408424068284	26.411644936243984	26.423746056582587	23.270524766490592
54-55	23.318504906185517	26.303373719280497	26.746573198092825	23.631548176441164
56-57	23.509262340699628	26.48623471726097	26.459639215840784	23.54486372619861
58-59	23.475049657236656	26.435803025942782	26.592074683921048	23.49707263289951
60-61	23.528304634801717	26.465820333800288	26.60010174006469	23.40577329133331
62-63	23.527166230039526	26.391419168388897	26.6453384335324	23.436076168039175
64-65	23.253438263278962	26.24192888303025	26.659344936199954	23.845287917490833
66-67	23.270209191533805	26.531740485103644	26.462239882999693	23.735810440362854
68-69	23.483453812547854	26.30401860108994	26.56295742692429	23.649570159437914
70-71	23.34712168945722	26.24302839880515	26.536356495519314	23.873493416218317
72-73	23.403055237411614	26.384964136234807	26.376097156760807	23.83588346959277
74-75	23.22172743662749	26.496718605079934	26.36480835893661	23.916745599355966
76-77	23.231648984655138	26.150088877276012	26.5931882029653	24.025073935103546
78-79	23.209793798828365	26.21642780354109	26.545910368070043	24.0278680295605
80-81	23.39649630134638	26.361754615793707	26.27309392718279	23.96865515567712
82-83	23.28878411779711	26.438203136682294	26.334937152005068	23.938075593515528
84-85	23.27084768736721	26.46851003544904	26.330359423835237	23.930282853348515
86-87	23.186941401213787	26.53210889019232	26.247568095097602	24.03338161349629
88-89	23.04220835448119	26.40638375393315	26.239517042378363	24.311890849207305
90-91	23.046758516378134	26.234407681582333	26.378566157800776	24.34026764423876
92-93	23.055055978094458	26.245332225855154	26.465865856705932	24.233745939344452
94-95	22.974360634076792	26.061089396018673	26.508634109154894	24.455915860749638
96-97	22.909117518339592	25.961482984600792	26.51986783390865	24.609531663150964
98-99	22.757127223261325	26.02810611078728	26.45618098396364	24.758585681987753
100-101	22.75032679922497	26.033304675374747	26.48635604441374	24.730012480986545
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	41.0
1	32.5
2	37.5
3	57.0
4	49.0
5	45.0
6	107.5
7	257.0
8	395.0
9	408.5
10	320.5
11	254.5
12	280.0
13	309.5
14	341.0
15	383.0
16	405.0
17	447.0
18	515.0
19	618.5
20	700.5
21	781.5
22	961.5
23	1209.5
24	1536.5
25	2017.0
26	2656.0
27	3572.5
28	4669.5
29	6203.0
30	8532.0
31	11442.0
32	15379.0
33	18191.5
34	20097.5
35	23134.5
36	27290.5
37	31401.5
38	34740.5
39	37994.0
40	40437.5
41	42740.0
42	45697.0
43	49281.0
44	52414.0
45	55922.5
46	58329.0
47	59632.0
48	61478.5
49	61790.0
50	61539.0
51	60699.5
52	59553.5
53	58464.5
54	57020.5
55	54007.5
56	48973.5
57	43848.0
58	39285.5
59	34314.5
60	28083.5
61	22420.0
62	18106.5
63	13589.0
64	9526.0
65	6847.5
66	5053.0
67	3890.0
68	2929.0
69	2210.5
70	1623.5
71	1137.5
72	790.0
73	584.5
74	446.0
75	322.5
76	237.0
77	145.0
78	78.0
79	50.0
80	31.0
81	17.0
82	11.0
83	7.0
84	6.0
85	5.5
86	3.5
87	2.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.5
93	1.5
94	1.5
95	0.5
96	0.5
97	0.5
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1478140961600698
2	7.73895791413978E-4
3	0.006965062122725802
4	0.004221249771348971
5	0.030041227539433505
6	0.0013367290942605074
7	0.0
8	0.001899562397107037
9	0.0
10-11	0.1003953903952497
12-13	1.0553124428372426E-4
14-15	0.005065499725618765
16-17	1.4070832571163236E-4
18-19	3.86947895706989E-4
20-21	6.683645471302537E-4
22-23	3.165937328511728E-4
24-25	0.001618145745683772
26-27	0.0032011144099396358
28-29	0.006965062122725802
30-31	0.015794509561130734
32-33	0.01582968664255864
34-35	0.011432551464070129
36-37	7.035416285581618E-4
38-39	3.165937328511728E-4
40-41	0.005311739295614121
42-43	0.00882944743840493
44-45	0.008266614135558402
46-47	0.009919936962670082
48-49	0.012558218069763188
50-51	0.006226343412739732
52-53	0.0016884999085395882
54-55	7.73895791413978E-4
56-57	0.006120812168456008
58-59	0.009638520311246816
60-61	0.007809312076995595
62-63	0.01822172817965639
64-65	0.03486048769505692
66-67	0.03721735215072676
68-69	0.027297415188056676
70-71	0.04333816431918276
72-73	0.026558696478070607
74-75	0.023779707045265868
76-77	0.2401539349083285
78-79	0.06831389213299752
80-81	0.05596673655180177
82-83	0.020578592635326232
84-85	0.03285539405366616
86-87	0.08421393293841196
88-89	0.26604426683926885
90-91	0.3191616597954101
92-93	0.051710309699024896
94-95	0.004889614318479224
96-97	0.009708874474102633
98-99	0.0011608436871209668
100-101	0.0012663749314046912
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1421380.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.56163611470409
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.68574401430169	53.55451024824516
2	10.530883258202339	13.808438722813824
3	3.2172691661929784	6.327882910710045
4	1.4500010556630856	3.8025776630928
5	0.8300041385973518	2.7208214654209
6	0.5365067448169978	2.110455598606585
7	0.3563088486349806	1.6352133755059044
8	0.2548486201863777	1.3366634000794984
9	0.1931432156239151	1.1396506698683058
>10	0.8954293515514048	9.855638519414782
>50	0.035753265697207604	1.5750433342201935
>100	0.013031382359988025	1.5968528243270976
>500	9.692443545330508E-4	0.44975912774407756
>1k	1.0769381717033898E-4	0.08649213995078414
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	7.035416285581618E-5	0.0
5	0.0	0.0	0.0	7.035416285581618E-5	0.0
6	0.0	0.0	0.0	7.035416285581618E-5	0.0
7	0.0	0.0	7.035416285581618E-5	7.035416285581618E-5	0.0
8	0.0	0.0	7.035416285581618E-5	7.035416285581618E-5	0.0
9	0.0	0.0	7.035416285581618E-5	7.035416285581618E-5	0.0
10-11	0.0	0.0	7.035416285581618E-5	7.035416285581618E-5	0.0
12-13	0.0	0.0	7.035416285581618E-5	7.035416285581618E-5	0.0
14-15	0.0	0.0	7.035416285581618E-5	7.035416285581618E-5	0.0
16-17	0.0	0.0	7.035416285581618E-5	1.4070832571163236E-4	0.0
18-19	0.0	0.0	7.035416285581618E-5	2.1106248856744855E-4	0.0
20-21	0.0	0.0	7.035416285581618E-5	3.165937328511728E-4	7.035416285581618E-5
22-23	0.0	0.0	7.035416285581618E-5	4.924791399907132E-4	7.035416285581618E-5
24-25	0.0	0.0	7.035416285581618E-5	8.090728728418861E-4	7.035416285581618E-5
26-27	0.0	0.0	7.035416285581618E-5	9.497811985535184E-4	7.035416285581618E-5
28-29	0.0	0.0	7.035416285581618E-5	0.0024623956999535665	7.035416285581618E-5
30-31	0.0	0.0	7.035416285581618E-5	0.00918121825268401	7.035416285581618E-5
32-33	0.0	0.0	7.035416285581618E-5	0.0217394363224472	7.035416285581618E-5
34-35	0.0	0.0	7.035416285581618E-5	0.0417551956549269	7.035416285581618E-5
36-37	0.0	0.0	7.035416285581618E-5	0.07446988138288144	7.035416285581618E-5
38-39	0.0	0.0	7.035416285581618E-5	0.1281852847232971	7.035416285581618E-5
40-41	0.0	0.0	7.035416285581618E-5	0.1856646357764989	7.035416285581618E-5
42-43	0.0	0.0	7.035416285581618E-5	0.24944068440529626	7.035416285581618E-5
44-45	0.0	0.0	7.035416285581618E-5	0.3221165346353544	1.0553124428372426E-4
46-47	0.0	0.0	7.035416285581618E-5	0.40833556121515713	2.814166514232647E-4
48-49	0.0	0.0	7.035416285581618E-5	0.5004643374748484	3.517708142790809E-4
50-51	0.0	0.0	7.035416285581618E-5	0.6017039778243679	3.517708142790809E-4
52-53	0.0	3.517708142790809E-5	7.035416285581618E-5	0.7049135347338502	4.5730205856280514E-4
54-55	0.0	7.035416285581618E-5	7.035416285581618E-5	0.817163601570305	5.628333028465294E-4
56-57	0.0	7.035416285581618E-5	7.035416285581618E-5	0.9492887194135277	5.628333028465294E-4
58-59	0.0	7.035416285581618E-5	7.035416285581618E-5	1.0893638576594578	5.628333028465294E-4
60-61	0.0	7.035416285581618E-5	7.035416285581618E-5	1.2411177869394532	5.628333028465294E-4
62-63	0.0	7.035416285581618E-5	7.035416285581618E-5	1.3994146533650396	5.628333028465294E-4
64-65	0.0	7.035416285581618E-5	7.035416285581618E-5	1.569179248336124	5.980103842744375E-4
66-67	0.0	7.035416285581618E-5	7.035416285581618E-5	1.7711308728137443	6.331874657023456E-4
68-69	0.0	7.035416285581618E-5	7.035416285581618E-5	1.9900026734581884	6.331874657023456E-4
70-71	0.0	7.035416285581618E-5	7.035416285581618E-5	2.222171410882382	6.331874657023456E-4
72-73	0.0	7.035416285581618E-5	7.035416285581618E-5	2.4691145225062963	6.331874657023456E-4
74-75	0.0	7.035416285581618E-5	7.035416285581618E-5	2.7257665086043144	6.331874657023456E-4
76-77	0.0	7.035416285581618E-5	7.035416285581618E-5	3.010489805681802	6.331874657023456E-4
78-79	0.0	7.035416285581618E-5	7.035416285581618E-5	3.3310937258157565	6.331874657023456E-4
80-81	0.0	7.035416285581618E-5	7.035416285581618E-5	3.6789950611377673	6.331874657023456E-4
82-83	0.0	7.035416285581618E-5	7.035416285581618E-5	4.033333802361086	7.035416285581618E-4
84-85	0.0	7.035416285581618E-5	7.035416285581618E-5	4.405999803008344	7.035416285581618E-4
86-87	0.0	7.035416285581618E-5	7.035416285581618E-5	4.808390437462185	7.035416285581618E-4
88-89	0.0	7.035416285581618E-5	7.035416285581618E-5	5.240822299455459	7.035416285581618E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1265	0.0	49.968807	1
GTATCAA	2210	0.0	41.29019	1
TCAACGC	2890	0.0	31.205261	4
CAACGCA	2915	0.0	30.500668	5
TATCAAC	2975	0.0	30.313684	2
ATCAACG	3020	0.0	29.861988	3
AACGCAG	3075	0.0	29.064157	6
GTACATG	3915	0.0	25.371868	1
TACATGG	4080	0.0	23.616087	2
ACATGGG	4080	0.0	23.03441	3
ACGCAGA	3900	0.0	22.306505	7
CGCAGAG	3935	0.0	21.98729	8
TATACTG	620	0.0	20.705149	5
CATGGGG	3545	0.0	20.485533	4
GTATTAC	260	1.4812031E-8	20.107462	1
GTGGTAT	695	0.0	19.831295	1
TACACTG	1465	0.0	19.796959	5
CTACACG	290	2.4629117E-9	19.640625	4
GAGTACA	2910	0.0	19.43533	1
CGTATAC	125	0.0060576787	18.985937	3
>>END_MODULE