FastQCFastQC Report
Mon 15 Aug 2016
SRR2921395_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921395_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2808693
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39840.14184533517903167No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31090.11069205498785378No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26400.053.0309681
GTATCAA52550.044.884371
TCAACGC63500.036.321714
CAACGCA65500.035.282595
ATCAACG66000.034.8746033
AACGCAG68050.033.670736
TATCAAC68900.033.4779972
ACGCAGA81750.027.3360847
GTACATG75550.027.0112951
CGCAGAG82400.026.6621788
TACATGG78600.025.6026952
ACATGGG78950.024.6458153
CATGGGG67100.023.198244
GCAGAGT95950.021.9573449
ATGGGGG47500.020.3802415
GAGTACA57150.020.0130041
AGAGTAC83050.019.85476510-11
GAGTACT53450.018.8211212-13
CAGAGTA88950.018.37777110-11
AGTACTT60000.017.43872312-13
Produced by FastQC (version 0.11.5)