##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921395_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2808693
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.360660990717033	31.0	31.0	34.0	26.0	34.0
2	30.413513687683203	31.0	31.0	34.0	26.0	34.0
3	30.457029301529218	33.0	31.0	34.0	26.0	34.0
4	33.924054711568694	37.0	35.0	37.0	30.0	37.0
5	33.926663398242525	37.0	35.0	37.0	30.0	37.0
6	33.977952022524356	37.0	35.0	37.0	30.0	37.0
7	33.894304575117324	37.0	35.0	37.0	30.0	37.0
8	33.86507425339829	37.0	35.0	37.0	29.0	37.0
9	35.41974790409632	39.0	37.0	39.0	30.0	39.0
10-11	35.3150746628414	39.0	36.0	39.0	29.0	39.0
12-13	35.20197259009795	39.0	35.0	39.0	29.0	39.0
14-15	36.32320477887758	40.0	37.0	41.0	28.0	41.0
16-17	36.281443539753184	40.0	37.0	41.0	28.0	41.0
18-19	36.25364662496044	40.0	37.0	41.0	27.0	41.0
20-21	36.20424393125201	40.0	37.0	41.0	27.0	41.0
22-23	36.095399176770115	40.0	36.0	41.0	27.0	41.0
24-25	35.99975344403963	40.0	36.0	41.0	27.0	41.0
26-27	35.85472068325018	40.0	36.0	41.0	27.0	41.0
28-29	35.71542689072818	40.0	36.0	41.0	27.0	41.0
30-31	35.55415080964705	40.0	36.0	41.0	25.5	41.0
32-33	35.35085019971923	39.5	35.0	41.0	25.0	41.0
34-35	35.18214005589076	39.0	35.0	41.0	24.5	41.0
36-37	35.01010309777537	39.0	35.0	41.0	24.0	41.0
38-39	35.20699538895849	39.5	35.0	41.0	24.5	41.0
40-41	35.31308886375264	40.0	35.0	41.0	24.5	41.0
42-43	35.1365252450161	40.0	35.0	41.0	23.5	41.0
44-45	34.997669734641704	40.0	35.0	41.0	23.0	41.0
46-47	34.81896045598433	40.0	35.0	41.0	21.5	41.0
48-49	34.65811785054471	40.0	35.0	41.0	19.5	41.0
50-51	32.96367545331583	37.0	32.5	39.5	15.5	40.0
52-53	33.12156472779332	37.5	33.0	39.5	14.0	40.0
54-55	33.992911293615926	39.0	34.0	40.5	14.0	41.0
56-57	34.02518270953786	39.0	34.0	41.0	13.0	41.0
58-59	33.86613257483107	39.0	34.0	41.0	12.0	41.0
60-61	33.63961654050478	38.0	34.0	41.0	8.5	41.0
62-63	33.36768276205338	38.0	34.0	40.0	7.5	41.0
64-65	33.10138327684798	37.0	33.0	40.0	4.5	41.0
66-67	32.775067086363656	37.0	33.0	40.0	2.0	41.0
68-69	32.424114525866656	36.0	33.0	39.0	2.0	41.0
70-71	32.049706037648114	36.0	32.5	39.0	2.0	41.0
72-73	31.69423731963586	35.0	32.0	38.5	2.0	40.5
74-75	31.298994763756667	35.0	32.0	37.0	2.0	39.5
76-77	30.91754064969009	35.0	32.0	37.0	2.0	39.0
78-79	30.559039204355905	35.0	31.0	36.5	2.0	39.0
80-81	30.19473149966906	35.0	31.0	36.0	2.0	38.0
82-83	29.879180102631366	35.0	31.0	36.0	2.0	37.0
84-85	29.62439451374714	35.0	31.0	35.0	2.0	37.0
86-87	29.38775935995853	35.0	31.0	35.0	2.0	36.0
88-89	29.173272942254634	35.0	31.0	35.0	2.0	36.0
90-91	28.912539569116312	35.0	30.0	35.0	2.0	36.0
92-93	28.711945734190245	34.0	30.0	35.0	2.0	35.5
94-95	28.499554597102637	34.0	30.0	35.0	2.0	35.0
96-97	28.246082964567506	34.0	30.0	35.0	2.0	35.0
98-99	28.026564491028388	34.0	29.0	35.0	2.0	35.0
100-101	26.997260291530615	33.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	98725.0
3	16417.0
4	11128.0
5	8244.0
6	7575.0
7	8071.0
8	8407.0
9	9076.0
10	9765.0
11	11000.0
12	11024.0
13	11741.0
14	12716.0
15	14442.0
16	15375.0
17	17232.0
18	14991.0
19	12628.0
20	12512.0
21	12875.0
22	13888.0
23	15647.0
24	18024.0
25	20798.0
26	24913.0
27	29665.0
28	34683.0
29	40828.0
30	48642.0
31	58748.0
32	72927.0
33	94491.0
34	132300.0
35	189506.0
36	305697.0
37	564717.0
38	670143.0
39	149084.0
40	48.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.60400789667355	18.909270184642807	13.311423460187841	24.1752984584958
2	15.72325296397629	20.156178750569996	40.06636884404925	24.05419944140447
3	18.95866869519405	24.407480378770853	30.194055407864735	26.439795518170367
4	13.0770935248108	16.097843883546055	37.628015406735244	33.197047184907895
5	13.678181514428617	37.677585924996336	34.89975966256581	13.744472898009228
6	32.42321037877752	36.1978454464652	17.464332923104973	13.914611251652309
7	27.860482506899313	32.7390723760349	21.539642125879105	17.860802991186684
8	26.015449779314302	35.55739074508932	20.218435036783323	18.208724438813046
9	26.59866557078614	15.939684312337347	19.997314902788084	37.46433521408843
10-11	25.03796188142788	25.796113358593768	28.497603344634054	20.668321415344295
12-13	25.647584480373432	23.39598538488783	28.340792306606094	22.61563782813265
14-15	22.89505249912954	25.04401271270585	25.729943159707595	26.330991628457014
16-17	22.75740500771677	28.22504720254473	26.559589350771134	22.457958438967374
18-19	22.745155717013354	27.34805031220325	28.255805278190927	21.650988692592467
20-21	23.058338348890466	26.543824098179087	28.421524040841472	21.97631351208898
22-23	22.95078843059255	26.298111987689314	28.121364968515316	22.629734613202817
24-25	22.839874569828904	26.492769171832816	28.090495371525527	22.576860886812753
26-27	22.655853785707368	26.785866920433758	28.005601490691724	22.55267780316715
28-29	22.63151381664051	27.121105153185365	27.845629678957522	22.401751351216607
30-31	23.12585582491507	26.64230698082507	27.806095333932625	22.425741860327236
32-33	22.810028531986053	26.662719023852144	27.76313929470127	22.764113149460535
34-35	23.104808831389132	26.86640291379155	27.72474511623369	22.304043138585627
36-37	23.05957972084167	26.79004591874337	27.844233026674313	22.306141333740644
38-39	22.93622031880227	26.904656273499988	27.741202458810932	22.417920948886813
40-41	23.034598388734025	26.73941705455282	27.753089707715482	22.47289484899767
42-43	22.850640264045246	26.78798179488035	27.53230848503523	22.829069456039175
44-45	22.768088790378783	26.859831618354917	27.691733053071843	22.680346538194456
46-47	22.969720829980446	26.830996882373416	27.338322511040662	22.860959776605476
48-49	22.777860526346302	27.119490617650182	27.27798137889638	22.824667477107138
50-51	22.921002704903	27.046122484800527	27.394884444060736	22.637990366235734
52-53	23.154178883067196	26.859146477645147	27.317827869875977	22.66884676941168
54-55	22.579176673901845	26.78547984068339	27.701632410170347	22.93371107524442
56-57	22.729761422441822	26.93801115256558	27.33008322232326	23.002144202669346
58-59	22.80342867764996	26.898643003898105	27.49920926482947	22.79871905362246
60-61	22.89080471458681	26.918498790953183	27.64390904085854	22.546787453601468
62-63	22.8398299351767	26.812290737204464	27.69499439111484	22.652884936503995
64-65	22.53762645922727	26.70615146491825	27.664608400096764	23.09161367575772
66-67	22.604115103011363	26.901471387021076	27.479002164670124	23.01541134529744
68-69	22.622106868594972	26.84647561965412	27.553902549969226	22.97751496178168
70-71	22.579308679398196	26.696898351162783	27.524964236164557	23.198828733274464
72-73	22.553043484464865	26.912780467619196	27.3846982556807	23.149477792235242
74-75	22.526342750776067	26.9656554313262	27.458264335743166	23.049737482154566
76-77	22.54841982325079	26.648542750064745	27.444035481116142	23.35900194556832
78-79	22.456289587625903	26.6883598932335	27.564075688534555	23.29127483060604
80-81	22.696825758767943	26.856422438541514	27.245436758365067	23.201315044325472
82-83	22.7113949499228	26.85707791218371	27.394675869343587	23.036851268549903
84-85	22.62151095886745	26.882811036476035	27.372815585944814	23.122862418711698
86-87	22.495099942612583	27.04622899006712	27.279668723287013	23.17900234403328
88-89	22.457078945048703	26.76188936508565	27.407895539821908	23.373136150043734
90-91	22.6177016820338	26.568796652777003	27.461418001744413	23.352083663444777
92-93	22.685520514194543	26.491497335182746	27.541149999759096	23.281832150863615
94-95	22.665490930519848	26.398863669217665	27.562999504934417	23.372645895328073
96-97	22.524282275473723	26.212168478943703	27.662501713287114	23.601047532295464
98-99	22.34734439999886	26.20426006663983	27.63290134507382	23.81549418828749
100-101	22.555736957113005	26.139456789982447	27.731398230037957	23.57340802286659
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	150.0
1	132.0
2	161.0
3	238.0
4	230.0
5	228.5
6	364.0
7	776.0
8	1263.5
9	1449.5
10	1275.5
11	1087.0
12	1113.5
13	1208.0
14	1354.0
15	1565.5
16	1769.0
17	1998.0
18	2243.5
19	2446.5
20	2729.5
21	3120.5
22	3611.5
23	4184.5
24	5108.5
25	6661.0
26	8894.5
27	11841.5
28	15527.5
29	20297.0
30	26173.5
31	32797.5
32	41010.0
33	47194.0
34	51408.0
35	56832.5
36	64296.5
37	72571.0
38	78863.5
39	84265.0
40	88426.0
41	92520.0
42	96760.0
43	100880.0
44	105496.5
45	110878.0
46	113564.0
47	114388.5
48	115977.0
49	115532.5
50	113558.5
51	111469.0
52	108372.5
53	104047.0
54	98758.5
55	91708.5
56	83210.5
57	73606.0
58	64812.5
59	55071.5
60	44189.5
61	35756.0
62	29161.5
63	22020.5
64	15491.5
65	11215.0
66	8361.5
67	6384.5
68	4785.5
69	3531.5
70	2670.0
71	2010.0
72	1474.0
73	1085.5
74	790.0
75	590.0
76	448.0
77	315.5
78	202.0
79	140.5
80	111.0
81	90.5
82	66.0
83	55.0
84	54.5
85	46.0
86	44.0
87	40.5
88	31.0
89	24.5
90	21.5
91	18.0
92	14.5
93	11.5
94	10.0
95	7.5
96	6.5
97	4.5
98	3.0
99	4.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05109137951353174
2	0.05885299674973377
3	0.04991645580346446
4	0.057286431802977406
5	0.04948921081798544
6	0.01783747814374871
7	0.01577246071393349
8	0.022323550491278328
9	0.02139785302274047
10-11	0.021825098008219482
12-13	0.027681914684160928
14-15	0.0450565440936407
16-17	0.030138573350665238
18-19	0.02372989856848007
20-21	0.027165660326707117
22-23	0.048972956460531646
24-25	0.04395282788115326
26-27	0.030939657698438386
28-29	0.02048995742859757
30-31	0.01297756643392496
32-33	0.009470597178118078
34-35	0.009114559690218902
36-37	0.0012817349564370332
38-39	0.006444278530975084
40-41	0.003346752386252253
42-43	0.22410779675813625
44-45	0.19925638010277377
46-47	0.20505979115553033
48-49	0.20260313248902603
50-51	0.20062712443118558
52-53	0.20532681927145474
54-55	0.20368904682711853
56-57	0.21431676584090892
58-59	0.210827598459497
60-61	0.21618596265237958
62-63	0.21351568149313577
64-65	0.21401413397619465
66-67	0.20384926369667314
68-69	0.20358223558074878
70-71	0.18629661554324378
72-73	0.1940582327794458
74-75	0.19421844964900045
76-77	0.19222463971676507
78-79	0.21819757445900992
80-81	0.22622621981113633
82-83	0.22501569235227917
84-85	0.2196039225362117
86-87	0.23801106066059907
88-89	0.23605285447715363
90-91	0.23530517575256535
92-93	0.2392037862450613
94-95	0.25079280647617946
96-97	0.2513980702056081
98-99	0.24085936056379248
100-101	0.2510420327177089
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2808693.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.589039206602514
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.23024207685339	49.41303524826534
2	11.054752420412257	13.617031604801088
3	3.531813306401964	6.525629645951731
4	1.6275050762959276	4.0094589581173805
5	0.895509071627917	2.7576771661180004
6	0.5690328756136821	2.10277128516101
7	0.3945236297946885	1.7008831912349347
8	0.286595025958328	1.4120889832131782
9	0.2136601487231456	1.1843210948938505
>10	1.123560057625966	12.054240688881103
>50	0.05328563964452566	2.1956691840004905
>100	0.01783859680878533	1.947601535685002
>500	0.0012180537596158504	0.5422070241640461
>1k	4.64020479818051E-4	0.5373843895128082
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3984	0.14184533517903167	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3109	0.11069205498785378	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.560374878991759E-5
2	0.0	0.0	0.0	3.560374878991759E-5	3.560374878991759E-5
3	0.0	0.0	0.0	3.560374878991759E-5	3.560374878991759E-5
4	0.0	0.0	0.0	3.560374878991759E-5	3.560374878991759E-5
5	0.0	0.0	0.0	3.560374878991759E-5	3.560374878991759E-5
6	0.0	0.0	0.0	3.560374878991759E-5	3.560374878991759E-5
7	0.0	0.0	0.0	7.120749757983517E-5	3.560374878991759E-5
8	0.0	0.0	0.0	7.120749757983517E-5	3.560374878991759E-5
9	0.0	0.0	0.0	7.120749757983517E-5	7.120749757983517E-5
10-11	0.0	0.0	0.0	7.120749757983517E-5	7.120749757983517E-5
12-13	0.0	0.0	0.0	7.120749757983517E-5	1.0681124636975277E-4
14-15	0.0	1.7801874394958794E-5	0.0	1.4241499515967035E-4	1.2461312076471155E-4
16-17	0.0	3.560374878991759E-5	0.0	1.7801874394958794E-4	1.4241499515967035E-4
18-19	0.0	3.560374878991759E-5	0.0	1.7801874394958794E-4	1.4241499515967035E-4
20-21	0.0	3.560374878991759E-5	0.0	2.314243671344643E-4	1.4241499515967035E-4
22-23	0.0	3.560374878991759E-5	0.0	3.382356135042171E-4	1.9582061834454671E-4
24-25	0.0	3.560374878991759E-5	0.0	6.05263729428599E-4	2.492262415294231E-4
26-27	0.0	3.560374878991759E-5	0.0	0.0010859143380924864	2.492262415294231E-4
28-29	0.0	3.560374878991759E-5	0.0	0.0028839036519833247	2.492262415294231E-4
30-31	0.0	3.560374878991759E-5	0.0	0.01253251957405099	2.492262415294231E-4
32-33	0.0	3.560374878991759E-5	0.0	0.03327170324417799	2.492262415294231E-4
34-35	0.0	7.120749757983517E-5	0.0	0.0599211092134313	2.848299903193407E-4
36-37	0.0	7.120749757983517E-5	0.0	0.09993972285329866	2.848299903193407E-4
38-39	0.0	7.120749757983517E-5	0.0	0.16582445998904116	2.848299903193407E-4
40-41	0.0	7.120749757983517E-5	0.0	0.2395954274817504	2.848299903193407E-4
42-43	0.0	7.120749757983517E-5	0.0	0.3160722798824934	3.026318647142995E-4
44-45	0.0	7.120749757983517E-5	0.0	0.4131459009582037	3.560374878991759E-4
46-47	0.0	7.120749757983517E-5	0.0	0.5267574633468307	3.916412366890935E-4
48-49	0.0	7.120749757983517E-5	0.0	0.6444456549719033	3.916412366890935E-4
50-51	0.0	7.120749757983517E-5	0.0	0.7669403526836147	3.916412366890935E-4
52-53	0.0	7.120749757983517E-5	0.0	0.8974458938730576	4.272449854790111E-4
54-55	0.0	7.120749757983517E-5	0.0	1.0551883028867874	4.984524830588462E-4
56-57	0.0	7.120749757983517E-5	0.0	1.2357705167492496	5.340562318487638E-4
58-59	0.0	7.120749757983517E-5	0.0	1.4300423720214348	5.340562318487638E-4
60-61	0.0	7.120749757983517E-5	0.0	1.6335712019789987	5.340562318487638E-4
62-63	0.0	7.120749757983517E-5	0.0	1.849490136515454	6.05263729428599E-4
64-65	0.0	7.120749757983517E-5	0.0	2.0852759628766835	6.05263729428599E-4
66-67	0.0	7.120749757983517E-5	0.0	2.3660115220851834	6.05263729428599E-4
68-69	0.0	7.120749757983517E-5	0.0	2.6739305434947855	6.230656038235578E-4
70-71	0.0	7.120749757983517E-5	0.0	2.9927799157828927	6.764712270084342E-4
72-73	0.0	7.120749757983517E-5	0.0	3.3209930740027476	6.94273101403393E-4
74-75	0.0	7.120749757983517E-5	0.0	3.671921423950571	7.120749757983518E-4
76-77	0.0	7.120749757983517E-5	0.0	4.0788900744937235	7.120749757983518E-4
78-79	0.0	7.120749757983517E-5	0.0	4.529651336048476	7.120749757983518E-4
80-81	0.0	8.900937197479397E-5	0.0	4.999086763843539	7.298768501933106E-4
82-83	0.0	1.0681124636975277E-4	0.0	5.481268333705392	7.476787245882694E-4
84-85	0.0	1.0681124636975277E-4	0.0	5.98999249829013	7.476787245882694E-4
86-87	0.0	1.0681124636975277E-4	0.0	6.547992251200114	7.83282473378187E-4
88-89	0.0	1.0681124636975277E-4	0.0	7.157670845478663	8.366880965630634E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2640	0.0	53.030968	1
GTATCAA	5255	0.0	44.88437	1
TCAACGC	6350	0.0	36.32171	4
CAACGCA	6550	0.0	35.28259	5
ATCAACG	6600	0.0	34.874603	3
AACGCAG	6805	0.0	33.67073	6
TATCAAC	6890	0.0	33.477997	2
ACGCAGA	8175	0.0	27.336084	7
GTACATG	7555	0.0	27.011295	1
CGCAGAG	8240	0.0	26.662178	8
TACATGG	7860	0.0	25.602695	2
ACATGGG	7895	0.0	24.645815	3
CATGGGG	6710	0.0	23.19824	4
GCAGAGT	9595	0.0	21.957344	9
ATGGGGG	4750	0.0	20.380241	5
GAGTACA	5715	0.0	20.013004	1
AGAGTAC	8305	0.0	19.854765	10-11
GAGTACT	5345	0.0	18.82112	12-13
CAGAGTA	8895	0.0	18.377771	10-11
AGTACTT	6000	0.0	17.438723	12-13
>>END_MODULE