FastQCFastQC Report
Mon 15 Aug 2016
SRR2921395_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921395_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2808693
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37890.13490260416499775No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30020.10688245386733258No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26150.052.3516581
GTATCAA54700.045.535871
TCAACGC69600.034.8437354
ATCAACG71650.033.979283
CAACGCA71800.033.8381655
TATCAAC72700.033.619082
AACGCAG74750.032.435096
ACGCAGA87700.027.2661917
GTACATG68000.026.9828621
CGCAGAG89650.026.5140348
TACATGG72050.025.3594462
ACATGGG73000.024.5093353
CGTACGA1050.002208222222.5992342
GCAGAGT100800.022.30789
CATGGGG60850.021.7598864
TATAGCG1100.002893063221.5719952
AGAGTAC87300.021.04705810-11
GCGTACG1150.00370892620.6672081
ATGGGGG41950.018.814235
GAGTACT60350.018.47579212-13
Produced by FastQC (version 0.11.5)