##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921395_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2808693
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.381729865100958	33.0	31.0	34.0	29.0	34.0
2	31.508255263213176	34.0	31.0	34.0	28.0	34.0
3	31.578987806784152	34.0	31.0	34.0	28.0	34.0
4	35.07362962061001	37.0	35.0	37.0	32.0	37.0
5	34.81963283278023	37.0	35.0	37.0	32.0	37.0
6	34.77418037500004	37.0	35.0	37.0	32.0	37.0
7	34.64341919889429	37.0	35.0	37.0	32.0	37.0
8	34.60858164277833	37.0	35.0	37.0	32.0	37.0
9	36.223505381328614	39.0	37.0	39.0	32.0	39.0
10-11	36.07587675121489	39.0	37.0	39.0	32.0	39.0
12-13	36.006831469299065	39.0	37.0	39.0	31.0	39.0
14-15	37.241716876853395	40.0	38.0	41.0	31.0	41.0
16-17	37.230878917703	40.0	38.0	41.0	31.0	41.0
18-19	37.21282710499154	40.0	38.0	41.0	31.0	41.0
20-21	37.18510923052111	40.0	38.0	41.0	31.0	41.0
22-23	37.07868517491944	40.0	38.0	41.0	31.0	41.0
24-25	37.01262455526467	40.0	37.5	41.0	31.0	41.0
26-27	36.87480262171765	40.0	37.0	41.0	30.0	41.0
28-29	36.71739061549269	40.0	37.0	41.0	30.0	41.0
30-31	36.56089220146167	40.0	37.0	41.0	30.0	41.0
32-33	36.47762268784805	40.0	36.5	41.0	30.0	41.0
34-35	36.58075677904277	40.0	37.0	41.0	30.0	41.0
36-37	36.5444840358131	40.0	37.0	41.0	30.0	41.0
38-39	36.450427298391105	40.0	37.0	41.0	30.0	41.0
40-41	36.31160774780298	40.0	36.5	41.0	29.0	41.0
42-43	36.18953032602709	40.0	36.0	41.0	29.0	41.0
44-45	36.033530542497886	40.0	36.0	41.0	28.0	41.0
46-47	35.845676618982566	40.0	36.0	41.0	27.0	41.0
48-49	35.66641922061258	40.0	35.5	41.0	26.5	41.0
50-51	35.51644323534113	40.0	35.0	41.0	26.0	41.0
52-53	35.35179512321211	39.5	35.0	41.0	26.0	41.0
54-55	35.15511787867168	39.0	35.0	41.0	25.0	41.0
56-57	34.90776902281595	39.0	35.0	41.0	24.0	41.0
58-59	34.70293495942775	39.0	35.0	41.0	24.0	41.0
60-61	34.44810913830739	38.0	34.5	40.0	23.0	41.0
62-63	34.164447128967105	38.0	34.0	40.0	22.5	41.0
64-65	33.86326326871609	37.0	34.0	40.0	22.0	41.0
66-67	33.540255200550575	37.0	34.0	40.0	21.5	41.0
68-69	33.18620938635871	36.0	34.0	39.0	20.5	41.0
70-71	32.80833077876436	36.0	33.0	39.0	19.5	40.5
72-73	32.414207070690885	35.0	33.0	38.5	18.0	40.0
74-75	31.997139594822215	35.0	33.0	37.0	14.5	39.5
76-77	29.22355736280184	33.0	29.0	34.5	7.5	37.0
78-79	30.864112418124726	34.5	31.5	36.0	8.0	39.0
80-81	31.040372158865353	35.0	32.0	36.0	7.0	37.5
82-83	30.85335136307172	35.0	32.0	35.5	6.0	37.0
84-85	30.59856773239368	35.0	32.0	35.0	2.0	37.0
86-87	30.332607194876765	35.0	32.0	35.0	2.0	36.0
88-89	30.034234428611455	35.0	31.5	35.0	2.0	36.0
90-91	29.83435124451124	34.5	31.0	35.0	2.0	36.0
92-93	29.675841574711086	34.0	31.0	35.0	2.0	35.0
94-95	29.56186952436596	34.0	31.0	35.0	2.0	35.0
96-97	29.359313388825335	34.0	31.0	35.0	2.0	35.0
98-99	29.094966947259813	34.0	31.0	35.0	2.0	35.0
100-101	28.033366409215958	33.0	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	54636.0
3	17849.0
4	9687.0
5	6574.0
6	5917.0
7	6060.0
8	6318.0
9	6810.0
10	7600.0
11	8461.0
12	9434.0
13	10383.0
14	11619.0
15	13092.0
16	15095.0
17	17235.0
18	15402.0
19	12562.0
20	11636.0
21	11068.0
22	11456.0
23	12681.0
24	14617.0
25	17442.0
26	20410.0
27	24625.0
28	29893.0
29	35269.0
30	42818.0
31	53218.0
32	66822.0
33	87826.0
34	126232.0
35	185790.0
36	316222.0
37	629697.0
38	753446.0
39	122780.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.93156121868957	20.62153339671314	14.130475869196058	25.31642951540122
2	14.60181622813295	21.451088376809302	38.7339168106846	25.213178584373146
3	17.43790323729224	25.491575634141828	30.06829219660177	27.00222893196417
4	12.58308492714447	17.306624149211768	36.58553172217056	33.5247592014732
5	13.346004571332518	38.06157837043178	34.96057955778163	13.631837500454068
6	32.27178246692905	36.93165917673138	17.174394319279884	13.622164037059692
7	27.51394957360875	32.906716587958506	21.66488410247062	17.91444973596213
8	25.922894272203916	36.08141600356329	20.004792360137674	17.990897364095108
9	26.216162618102167	15.94041494774612	19.93191846308476	37.91150397106695
10-11	24.86783028528271	26.17910212629911	28.42793867995308	20.5251289084651
12-13	25.447758709730024	23.559382892376448	28.45516413650737	22.53769426138616
14-15	22.618336139956217	25.312818727055436	25.73449756999689	26.33434756299146
16-17	22.525343243062817	28.481755268252517	26.558739624824764	22.4341618638599
18-19	22.47765013206356	27.632303438743854	28.330441411115626	21.55960501807696
20-21	22.835032267011456	26.715102342796342	28.47390414015727	21.975961250034935
22-23	22.702403437907776	26.41957001230113	28.239443013367467	22.638583536423628
24-25	22.571655675353792	26.703166904473047	28.16422080655972	22.560956613613442
26-27	22.36736837420446	26.98621211446882	28.151337398193064	22.495082113133652
28-29	22.40598620485351	27.312424604525365	27.926733045542782	22.35485614507834
30-31	22.908186892632152	26.82471858619284	27.90871123577441	22.358383285400595
32-33	22.64878742720201	26.88430839462519	27.744708959382685	22.722195218790116
34-35	22.889989648774037	27.02629610203524	27.855248769478873	22.228465479711843
36-37	22.83009453028855	26.96164901867481	27.945279564948116	22.26297688608852
38-39	22.797490714972486	27.053996909945692	27.777234819738823	22.371277555343
40-41	22.891452156401414	26.85375607783041	27.777661071407206	22.47713069436097
42-43	22.66663580735959	26.957462225537622	27.50871033614687	22.867191630955922
44-45	22.61948337633427	26.994950268224716	27.65883581998144	22.726730535459573
46-47	22.78146577285974	26.98937647335092	27.321233718476456	22.907924035312888
48-49	22.594402986071113	27.279269398913424	27.31108497893353	22.81524263608193
50-51	22.762480354519475	27.185677652469227	27.34848501032923	22.70335698268207
52-53	23.01272175665702	27.070930442457648	27.210000665799583	22.70634713508575
54-55	22.428640932855835	26.989347788122153	27.5525868067949	23.029424472227113
56-57	22.57514577494339	27.125061575927145	27.221161859189763	23.078630789939698
58-59	22.636059893004713	27.084541372857508	27.342691667246765	22.936707066891014
60-61	22.688638776386924	27.156089820452028	27.437809098514332	22.717462304646713
62-63	22.675052133187474	26.984319448242854	27.497852710925745	22.842775707643924
64-65	22.311202952996805	26.867348784994537	27.46744338118704	23.35400488082162
66-67	22.406748885360415	27.10595469352487	27.2618332731403	23.225463147974416
68-69	22.419811782722114	27.088063249552608	27.306373923804944	23.185751043920334
70-71	22.402021841904958	26.883117206920364	27.292123101406606	23.422737849768072
72-73	22.37492984190568	27.177233591553108	27.076163199835896	23.37167336670532
74-75	22.338066379936944	27.240938081266048	27.04736524777358	23.373630291023428
76-77	22.277108283232774	26.882255212153332	27.19990078037307	23.64073572424082
78-79	22.208093478208976	26.93697895308026	27.22252486791564	23.632402700795126
80-81	22.400961397681574	27.143258157791333	26.90590108233829	23.5498793621888
82-83	22.420891969793335	27.132118903665862	26.996369815841337	23.450619310699462
84-85	22.3137439124374	27.142782859304205	26.92868086961415	23.614792358644248
86-87	22.121531306433752	27.30509822590462	26.875702860759098	23.697667606902538
88-89	22.08302915962382	27.05483694206807	26.9517599832935	23.91037391501461
90-91	22.113092846287238	26.936299328329287	26.991858158004238	23.958749667379234
92-93	22.19874490662676	26.827678739841083	27.074793170351324	23.89878318318083
94-95	22.127936767404837	26.77476272021361	27.0338464507416	24.06345406163996
96-97	21.923913900763438	26.602129567597043	27.121498085838947	24.352458445800572
98-99	21.666344447921794	26.654824682739154	27.040650475975838	24.638180393363218
100-101	21.63309724184904	26.70933118568768	27.061225347203305	24.596346225259975
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	134.0
1	115.5
2	151.5
3	205.0
4	191.5
5	199.5
6	321.5
7	712.0
8	1096.0
9	1180.5
10	1040.5
11	859.5
12	861.0
13	990.0
14	1110.0
15	1225.0
16	1393.5
17	1556.0
18	1826.5
19	2110.5
20	2417.5
21	2881.0
22	3334.0
23	4094.5
24	5210.5
25	6878.5
26	9304.0
27	12455.0
28	16383.0
29	21277.0
30	27200.5
31	33728.0
32	42195.0
33	48411.0
34	52048.0
35	57045.0
36	64731.0
37	72764.5
38	78822.5
39	84287.5
40	88553.5
41	92910.5
42	97189.0
43	101573.5
44	105820.0
45	110845.5
46	114152.0
47	115437.5
48	117056.5
49	116617.5
50	114149.0
51	111522.0
52	108502.0
53	104363.0
54	99211.0
55	91556.0
56	82602.0
57	72915.0
58	63721.5
59	54437.0
60	43581.5
61	34788.5
62	28387.5
63	21350.5
64	14776.0
65	10531.5
66	7701.0
67	5831.0
68	4429.0
69	3263.5
70	2383.0
71	1729.0
72	1227.0
73	890.5
74	704.0
75	514.0
76	320.0
77	188.5
78	109.5
79	62.5
80	34.0
81	18.5
82	11.5
83	10.0
84	9.0
85	5.5
86	4.0
87	6.0
88	5.0
89	1.5
90	0.5
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1579026258832845
2	5.340562318487639E-4
3	0.006159448540655743
4	0.004308053603580028
5	0.027023245331547447
6	0.0012461312076471156
7	1.7801874394958794E-4
8	0.001993809932235385
9	7.120749757983517E-5
10-11	0.10752332134555112
12-13	3.382356135042171E-4
14-15	0.00560759043441202
16-17	1.9582061834454671E-4
18-19	2.6702811592438196E-4
20-21	6.94273101403393E-4
22-23	2.848299903193407E-4
24-25	0.0012639330820420744
26-27	0.002421054917714396
28-29	0.0060170335454960725
30-31	0.014864565119790594
32-33	0.014526329506286375
34-35	0.011749237100672804
36-37	5.874618550336402E-4
38-39	1.6021686955462915E-4
40-41	0.004450468598739698
42-43	0.008829729699899562
44-45	0.008082050975311292
46-47	0.009132361564613861
48-49	0.011411001487168587
50-51	0.005660996057596897
52-53	0.0014241499515967036
54-55	9.791030917227336E-4
56-57	0.005999231671101114
58-59	0.008954342820664273
60-61	0.00795743785454658
62-63	0.01783747814374871
64-65	0.034500032577430145
66-67	0.03770436996852273
68-69	0.027343679070656705
70-71	0.0410867261035649
72-73	0.026667207843648275
74-75	0.025136246645681818
76-77	0.2431023967375573
78-79	0.06992576262339814
80-81	0.05447373564857391
82-83	0.020329740559042943
84-85	0.032719845137934266
86-87	0.08206664096076004
88-89	0.26304049605991114
90-91	0.31893838166008176
92-93	0.05310299132016208
94-95	0.005037930453773339
96-97	0.010058059033151719
98-99	0.0014953574491765387
100-101	0.0012817349564370332
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2808693.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.926967311140345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.56806522007011	48.47840908673894
2	11.500839723520894	14.014225717712439
3	3.6194723089620155	6.615704131551192
4	1.6581615238979923	4.041070118524946
5	0.9228964239692732	2.8114640127372112
6	0.5820527757491972	2.1277626264857905
7	0.4013642045346749	1.711773263868219
8	0.2952729141880058	1.4392066552478222
9	0.22744528747415926	1.2471796435509919
>10	1.1504324955606164	12.245244023431866
>50	0.05350329447316289	2.1895576345428034
>100	0.018793769556448913	2.008178062278775
>500	0.0012896992054945208	0.5714367290297115
>1k	4.103588380834276E-4	0.4987882942992433
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3789	0.13490260416499775	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3002	0.10688245386733258	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.560374878991759E-5
2	0.0	0.0	0.0	0.0	3.560374878991759E-5
3	0.0	0.0	0.0	0.0	3.560374878991759E-5
4	0.0	0.0	0.0	0.0	3.560374878991759E-5
5	0.0	0.0	0.0	0.0	3.560374878991759E-5
6	0.0	0.0	0.0	0.0	3.560374878991759E-5
7	0.0	0.0	0.0	0.0	3.560374878991759E-5
8	0.0	0.0	0.0	0.0	3.560374878991759E-5
9	0.0	0.0	0.0	0.0	3.560374878991759E-5
10-11	0.0	0.0	0.0	3.560374878991759E-5	3.560374878991759E-5
12-13	0.0	0.0	0.0	3.560374878991759E-5	3.560374878991759E-5
14-15	0.0	0.0	0.0	7.120749757983517E-5	5.340562318487638E-5
16-17	0.0	0.0	0.0	1.0681124636975277E-4	7.120749757983517E-5
18-19	0.0	0.0	0.0	1.0681124636975277E-4	7.120749757983517E-5
20-21	0.0	0.0	0.0	1.4241499515967035E-4	7.120749757983517E-5
22-23	0.0	0.0	0.0	2.314243671344643E-4	7.120749757983517E-5
24-25	0.0	0.0	0.0	4.984524830588462E-4	1.0681124636975277E-4
26-27	0.0	0.0	0.0	9.791030917227336E-4	1.0681124636975277E-4
28-29	0.0	0.0	0.0	0.002759290531218613	1.0681124636975277E-4
30-31	0.0	0.0	0.0	0.012301095206916527	1.0681124636975277E-4
32-33	0.0	0.0	0.0	0.03353873136010237	1.0681124636975277E-4
34-35	0.0	0.0	0.0	0.06045516544528007	1.0681124636975277E-4
36-37	0.0	0.0	0.0	0.10099003344260124	1.0681124636975277E-4
38-39	3.560374878991759E-5	1.7801874394958794E-5	0.0	0.16733761931261265	1.0681124636975277E-4
40-41	3.560374878991759E-5	3.560374878991759E-5	0.0	0.2421945011434144	1.0681124636975277E-4
42-43	3.560374878991759E-5	3.560374878991759E-5	0.0	0.3189917872832666	1.0681124636975277E-4
44-45	3.560374878991759E-5	3.560374878991759E-5	0.0	0.41620782335413664	1.0681124636975277E-4
46-47	3.560374878991759E-5	3.560374878991759E-5	0.0	0.5300330082355031	1.0681124636975277E-4
48-49	3.560374878991759E-5	3.560374878991759E-5	0.0	0.6483086617156093	1.2461312076471155E-4
50-51	3.560374878991759E-5	3.560374878991759E-5	0.0	0.7706787463065561	1.7801874394958794E-4
52-53	3.560374878991759E-5	3.560374878991759E-5	0.0	0.9008638537568898	2.848299903193407E-4
54-55	3.560374878991759E-5	3.560374878991759E-5	0.0	1.0589623002585187	3.738393622941347E-4
56-57	3.560374878991759E-5	3.560374878991759E-5	0.0	1.239918353483275	3.916412366890935E-4
58-59	3.560374878991759E-5	3.560374878991759E-5	0.0	1.434314821876225	3.916412366890935E-4
60-61	3.560374878991759E-5	3.560374878991759E-5	0.0	1.6383243024424528	3.916412366890935E-4
62-63	3.560374878991759E-5	3.560374878991759E-5	0.0	1.854261038853303	4.6284873426892867E-4
64-65	3.560374878991759E-5	3.560374878991759E-5	0.0	2.0902248839584816	4.6284873426892867E-4
66-67	3.560374878991759E-5	7.120749757983517E-5	0.0	2.3712274712829062	4.6284873426892867E-4
68-69	3.560374878991759E-5	7.120749757983517E-5	0.0	2.679360115185248	4.6284873426892867E-4
70-71	3.560374878991759E-5	7.120749757983517E-5	0.0	2.9987613455795987	4.6284873426892867E-4
72-73	3.560374878991759E-5	7.120749757983517E-5	0.0	3.330000822446597	5.340562318487638E-4
74-75	3.560374878991759E-5	7.120749757983517E-5	0.0	3.6848455847613106	5.696599806386814E-4
76-77	3.560374878991759E-5	7.120749757983517E-5	0.0	4.0957484495457495	5.696599806386814E-4
78-79	3.560374878991759E-5	7.120749757983517E-5	0.0	4.551707858423829	6.05263729428599E-4
80-81	3.560374878991759E-5	7.120749757983517E-5	0.0	5.028424252846431	6.408674782185166E-4
82-83	3.560374878991759E-5	7.120749757983517E-5	0.0	5.515269913799764	6.764712270084342E-4
84-85	3.560374878991759E-5	8.900937197479397E-5	0.0	6.028569160104006	6.764712270084342E-4
86-87	3.560374878991759E-5	1.0681124636975277E-4	0.0	6.593066597168149	6.94273101403393E-4
88-89	5.340562318487638E-5	1.0681124636975277E-4	0.0	7.209474299967992	7.298768501933106E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2615	0.0	52.351658	1
GTATCAA	5470	0.0	45.53587	1
TCAACGC	6960	0.0	34.843735	4
ATCAACG	7165	0.0	33.97928	3
CAACGCA	7180	0.0	33.838165	5
TATCAAC	7270	0.0	33.61908	2
AACGCAG	7475	0.0	32.43509	6
ACGCAGA	8770	0.0	27.266191	7
GTACATG	6800	0.0	26.982862	1
CGCAGAG	8965	0.0	26.514034	8
TACATGG	7205	0.0	25.359446	2
ACATGGG	7300	0.0	24.509335	3
CGTACGA	105	0.0022082222	22.599234	2
GCAGAGT	10080	0.0	22.3078	9
CATGGGG	6085	0.0	21.759886	4
TATAGCG	110	0.0028930632	21.571995	2
AGAGTAC	8730	0.0	21.047058	10-11
GCGTACG	115	0.003708926	20.667208	1
ATGGGGG	4195	0.0	18.81423	5
GAGTACT	6035	0.0	18.475792	12-13
>>END_MODULE