FastQCFastQC Report
Mon 15 Aug 2016
SRR2921394_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921394_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1557055
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20200.058.7435041
GTATCAA32700.036.8686451
TCAACGC39700.030.9666655
CAACGCA40900.029.8173776
ATCAACG41200.029.4955084
TATCAAC41000.029.1743972
GTGGTAT8500.028.4788531
AACGCAG43050.028.2198267
TGGTATC9000.024.2605442
GTACATG52600.023.7323761
TACATGG53100.023.0627142
ACGCAGA52400.022.2787768
CGCAGAG53800.021.3462039
TACTACG1353.815029E-421.0961232
ACATGGG55000.020.8838213
GAGTACT23500.018.8818812-13
CAGAGTA53250.018.58150710-11
CATGGGG47050.018.1604234
GCAGAGT58150.017.872369
TTGTACG1600.001196443517.8009994
Produced by FastQC (version 0.11.5)