##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921394_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1557055
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.8521837700017	33.0	31.0	34.0	28.0	34.0
2	30.917480114703718	33.0	31.0	34.0	27.0	34.0
3	30.954367700562923	34.0	31.0	34.0	27.0	34.0
4	34.40631255800213	37.0	35.0	37.0	32.0	37.0
5	34.40247325881231	37.0	35.0	37.0	32.0	37.0
6	34.4473708378959	37.0	35.0	37.0	31.0	37.0
7	34.388981121411895	37.0	35.0	37.0	31.0	37.0
8	34.35866491549753	37.0	35.0	37.0	31.0	37.0
9	35.97704063119158	39.0	37.0	39.0	31.0	39.0
10-11	35.88928361554345	39.0	37.0	39.0	31.0	39.0
12-13	35.7881985543221	39.0	37.0	39.0	30.0	39.0
14-15	36.986292070607654	40.0	38.0	41.0	31.0	41.0
16-17	36.92313309420669	40.0	38.0	41.0	31.0	41.0
18-19	36.87659202789882	40.0	38.0	41.0	30.0	41.0
20-21	36.824490785489274	40.0	38.0	41.0	30.0	41.0
22-23	36.71722321947523	40.0	37.0	41.0	30.0	41.0
24-25	36.61525732873919	40.0	37.0	41.0	30.0	41.0
26-27	36.47243963764928	40.0	37.0	41.0	30.0	41.0
28-29	36.33514776292424	40.0	37.0	41.0	29.0	41.0
30-31	36.1853881205224	40.0	36.5	41.0	29.0	41.0
32-33	36.00668537720247	40.0	36.0	41.0	28.0	41.0
34-35	35.837994804294	40.0	36.0	41.0	27.0	41.0
36-37	35.669148809772295	40.0	35.5	41.0	27.0	41.0
38-39	35.90827170523842	40.0	36.0	41.0	27.5	41.0
40-41	36.02042092283189	40.0	36.0	41.0	28.0	41.0
42-43	35.85653910748175	40.0	36.0	41.0	27.0	41.0
44-45	35.72241025525752	40.0	36.0	41.0	26.5	41.0
46-47	35.554498396010416	40.0	35.0	41.0	26.0	41.0
48-49	35.397372925169634	40.0	35.0	41.0	25.0	41.0
50-51	33.69044124966684	38.0	33.0	39.5	22.0	40.0
52-53	33.8581832369441	37.5	33.5	39.5	22.5	40.0
54-55	34.701855746906816	39.0	35.0	40.5	23.0	41.0
56-57	34.701376637305685	39.0	35.0	41.0	23.0	41.0
58-59	34.52172049156902	39.0	35.0	41.0	23.0	41.0
60-61	34.27523369437817	38.0	34.5	41.0	22.0	41.0
62-63	33.98712794345737	37.5	34.0	40.0	22.0	41.0
64-65	33.69103628323983	37.0	34.0	40.0	21.5	41.0
66-67	33.34620164348723	36.5	34.0	39.5	20.0	41.0
68-69	32.973336523115755	36.0	34.0	39.0	18.5	41.0
70-71	32.59969750586845	35.5	33.0	39.0	17.5	41.0
72-73	32.24178786234269	35.0	33.0	37.5	14.5	40.0
74-75	31.844746653136852	35.0	33.0	37.0	10.5	39.0
76-77	31.473980687901197	35.0	33.0	37.0	7.5	39.0
78-79	31.139948813625722	35.0	32.0	36.0	4.5	38.5
80-81	30.800606914977315	35.0	32.0	36.0	2.0	37.0
82-83	30.516315415961543	35.0	32.0	35.0	2.0	37.0
84-85	30.293834835635224	35.0	32.0	35.0	2.0	36.5
86-87	30.083371492978735	35.0	32.0	35.0	2.0	36.0
88-89	29.893370818628757	35.0	32.0	35.0	2.0	36.0
90-91	29.653938364412305	35.0	31.0	35.0	2.0	35.5
92-93	29.47534929723099	35.0	31.0	35.0	2.0	35.0
94-95	29.29440032625694	35.0	31.0	35.0	2.0	35.0
96-97	29.06472764288994	35.0	31.0	35.0	2.0	35.0
98-99	28.849579173503827	35.0	31.0	35.0	2.0	35.0
100-101	27.82853656421899	34.0	28.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	45100.0
3	7715.0
4	5315.0
5	4181.0
6	3759.0
7	3997.0
8	4079.0
9	4233.0
10	4761.0
11	4978.0
12	5081.0
13	5122.0
14	5788.0
15	6511.0
16	6630.0
17	7393.0
18	6651.0
19	5780.0
20	5726.0
21	5940.0
22	6611.0
23	7428.0
24	8646.0
25	10299.0
26	12353.0
27	14398.0
28	17063.0
29	19894.0
30	24033.0
31	29552.0
32	37131.0
33	49426.0
34	72314.0
35	109685.0
36	187720.0
37	352166.0
38	381371.0
39	68216.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.17720225830142	17.792765442616925	13.264019131439337	24.76601316764232
2	17.41383010577747	19.238044935185435	37.55974158991342	25.788383369123668
3	20.216370834784332	22.832661484629295	28.45839314007564	28.49257454051074
4	14.817520640128304	15.977184538071713	34.24446110775917	34.96083371404081
5	16.022600670515686	35.447637967790065	32.791082578285724	15.738678783408528
6	34.49455978201873	32.658522344429166	17.046294684522596	15.800623189029508
7	29.605416973904287	30.731897816407646	20.13342034473988	19.529264864948193
8	27.197153152921675	32.76019552803489	19.704716696321324	20.337934622722102
9	26.915509649570758	15.771082441281983	19.418488506909988	37.89491940223728
10-11	26.121807018873643	24.675482761145805	26.837582076781857	22.365128143198692
12-13	26.756458051691396	22.524995671988435	26.497291258958345	24.22125501736183
14-15	24.395639131655486	24.260237369087537	24.166146796712322	27.177976702544655
16-17	24.268801389629875	26.763704784593557	25.201848685674932	23.765645140101633
18-19	24.107047649391745	25.988953882007728	26.64602960277399	23.25796886582654
20-21	24.64911553797145	24.973806922167213	26.798650229426308	23.57842731043503
22-23	24.59179490737885	24.84824420732334	26.34337928411357	24.216581601184235
24-25	24.26028070962169	25.33733114169145	26.315378638044688	24.087009510642172
26-27	23.936169700201237	25.685624852445432	26.421285760401897	23.956919686951437
28-29	23.940315822403054	25.854450362001618	26.379932436642488	23.825301378952837
30-31	24.444811993371275	25.177536836966713	26.399612039618205	23.978039130043808
32-33	24.156860075142745	25.270898292668747	26.148537786702903	24.423703845485605
34-35	24.412971848083703	25.638155063555374	26.11168917920753	23.837183909153385
36-37	24.253639200315213	25.628653369842812	26.22690447281811	23.890802957023862
38-39	24.074772849962635	25.702982519410476	26.34134373140401	23.880900899222873
40-41	24.294679755466504	25.38022505379718	26.33368989383788	23.99140529689844
42-43	24.19171635321385	25.3693960893055	26.1158925644692	24.322994993011445
44-45	24.144255635146553	25.605870515850643	26.08886240262896	24.161011446373845
46-47	24.219607091407966	25.57562771555505	25.871430037138516	24.33333515589847
48-49	23.870131724694232	25.843364813978592	26.13359912415107	24.152904337176107
50-51	23.999816032982842	25.702733100201236	26.308859394845	23.98859147197092
52-53	24.27247575348026	25.499773889986383	26.08309269609978	24.144657660433577
54-55	23.849981458187326	25.490891676253842	26.24597604069743	24.4131508248614
56-57	23.920366874385024	25.722250975197635	25.99016294413027	24.367219206287068
58-59	23.92642062368586	25.67873787097909	26.260120316413577	24.13472118892147
60-61	24.007241293126658	25.565862732591	26.488941041296147	23.937954932986194
62-63	23.941237320322994	25.434364176277747	26.501650738264704	24.122747765134555
64-65	23.797492214232914	25.36892731075327	26.38379505403557	24.449785420978245
66-67	23.849385034993826	25.606731165088515	26.09178803005352	24.45209576986414
68-69	23.80888362137588	25.575412364253886	26.310230428678715	24.30547358569152
70-71	23.66961885282805	25.420573971880266	26.428915988114078	24.480891187177605
72-73	23.641311077042516	25.57087141527999	26.32429336340975	24.463524144267744
74-75	23.737022084847418	25.646576190970848	26.23665465262459	24.379747071557144
76-77	23.755838226729182	25.40708797628401	26.207805013277213	24.6292687837096
78-79	23.647047243334633	25.48308712552545	26.2967566447801	24.573108986359816
80-81	23.70806549057127	25.667127127809813	26.181912820357745	24.44289456126117
82-83	23.64916922061374	25.574899463968677	26.434614826607717	24.341316488809866
84-85	23.659941120915715	25.591396396744155	26.30673352324926	24.441928959090873
86-87	23.546201876798428	25.848670901388292	26.163636901789804	24.441490320023473
88-89	23.52438919636087	25.592224488896427	26.200526779231115	24.682859535511593
90-91	23.574856411927662	25.459702204144875	26.336624553504066	24.628816830423396
92-93	23.543157065697883	25.2958920645461	26.518067529151658	24.642883340604357
94-95	23.506458739424467	25.28011037742102	26.54299616265997	24.670434720494537
96-97	23.522980330429228	25.230919814910212	26.482119581183035	24.763980273477532
98-99	23.379638864473154	25.189209432095016	26.36793175205167	25.063219951380155
100-101	23.51805351056929	25.144954489760195	26.50118795111253	24.835804048557986
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	32.0
1	26.0
2	39.5
3	53.0
4	45.5
5	49.0
6	85.0
7	215.0
8	356.5
9	417.0
10	382.5
11	315.0
12	328.0
13	356.5
14	394.0
15	453.5
16	533.5
17	601.0
18	639.0
19	694.5
20	774.0
21	859.5
22	936.5
23	1040.5
24	1237.0
25	1519.5
26	1910.0
27	2481.0
28	3357.5
29	4646.0
30	6387.5
31	8960.5
32	12586.5
33	15533.5
34	18094.5
35	21332.5
36	25067.5
37	29229.5
38	33116.0
39	36884.5
40	40174.5
41	43266.5
42	46767.0
43	50156.5
44	53705.5
45	57750.0
46	60783.0
47	63509.0
48	66365.0
49	67981.5
50	68560.5
51	68672.0
52	68303.5
53	67637.0
54	66618.0
55	63550.0
56	58955.5
57	53462.0
58	47740.0
59	41907.0
60	35047.0
61	29283.5
62	24432.0
63	18925.0
64	14074.0
65	10657.5
66	8251.0
67	6539.0
68	5192.0
69	4140.0
70	3198.5
71	2355.0
72	1720.5
73	1308.0
74	1058.0
75	840.5
76	612.0
77	425.5
78	302.5
79	197.0
80	137.5
81	92.5
82	70.5
83	55.0
84	41.5
85	37.0
86	40.0
87	35.5
88	29.5
89	27.0
90	19.5
91	16.5
92	13.5
93	12.0
94	11.0
95	7.5
96	7.5
97	7.5
98	6.5
99	6.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04264460792971347
2	0.04938810767763502
3	0.04193814605135978
4	0.04765406488531234
5	0.04116746036588303
6	0.014386132795565987
7	0.01098227101804368
8	0.0157348327451503
9	0.016055951780765614
10-11	0.01804688980158055
12-13	0.02154708728978745
14-15	0.03699291290288397
16-17	0.024501382417448325
18-19	0.019845156401026295
20-21	0.02222143726457961
22-23	0.0389838509236989
24-25	0.034391848714399945
26-27	0.0274556775451092
28-29	0.01888179929418036
30-31	0.012523642388997176
32-33	0.009729906779143961
34-35	0.009312452032844056
36-37	0.0010596928175305304
38-39	0.006390268808744714
40-41	0.003179078452591591
42-43	0.17517043392815282
44-45	0.15224253478521954
46-47	0.15860069169040272
48-49	0.15728410364437992
50-51	0.15596751559835714
52-53	0.16068796542190222
54-55	0.15802267742629517
56-57	0.16868382940872353
58-59	0.165087296209832
60-61	0.16932606747995413
62-63	0.1669176747128393
64-65	0.16800947943393135
66-67	0.1602383987720408
68-69	0.15750888696931065
70-71	0.1461091612049671
72-73	0.15082961102851217
74-75	0.15070116341426604
76-77	0.1492240158504356
78-79	0.17192713166843818
80-81	0.18085424085854385
82-83	0.17735404337033694
84-85	0.17231247451117654
86-87	0.1874692929922193
88-89	0.18583158591058122
90-91	0.1842902145396277
92-93	0.18888221674892666
94-95	0.19999293538121646
96-97	0.1995112568277935
98-99	0.18990979766289565
100-101	0.20031405441683175
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1557055.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.33706270766665
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.18986609601589	60.9004762313756
2	9.693859361600367	14.024506250387741
3	2.762248345394838	5.994387953249245
4	1.187817152918727	3.4369281550369606
5	0.6136246560825133	2.219390261300557
6	0.3820833254923084	1.658327128541455
7	0.26969667084766513	1.3656345493809536
8	0.19383965210446308	1.1217432855610265
9	0.12794014113624805	0.8329332610980446
>10	0.557282144111681	6.432338301896694
>50	0.014518039040823045	0.7087245732856639
>100	0.006510957854508442	0.9469568190544784
>500	5.350930499073361E-4	0.20566350950633402
>1k	1.7836434996911204E-4	0.15198972032525815
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.284476142461249E-4
2	0.0	0.0	0.0	0.0	1.284476142461249E-4
3	0.0	0.0	0.0	0.0	1.284476142461249E-4
4	0.0	0.0	0.0	0.0	1.284476142461249E-4
5	0.0	6.422380712306245E-5	0.0	0.0	1.284476142461249E-4
6	0.0	6.422380712306245E-5	0.0	0.0	1.284476142461249E-4
7	0.0	6.422380712306245E-5	0.0	0.0	1.284476142461249E-4
8	0.0	6.422380712306245E-5	0.0	0.0	1.284476142461249E-4
9	0.0	6.422380712306245E-5	0.0	0.0	1.284476142461249E-4
10-11	0.0	6.422380712306245E-5	0.0	0.0	1.6055951780765613E-4
12-13	0.0	1.284476142461249E-4	0.0	0.0	1.9267142136918734E-4
14-15	0.0	1.284476142461249E-4	0.0	0.0	2.8900713205378103E-4
16-17	0.0	1.284476142461249E-4	0.0	0.0	3.853428427383747E-4
18-19	0.0	1.284476142461249E-4	0.0	0.0	3.853428427383747E-4
20-21	0.0	1.9267142136918734E-4	0.0	3.2111903561531224E-5	4.495666498614371E-4
22-23	0.0	1.9267142136918734E-4	0.0	6.422380712306245E-5	5.780142641075621E-4
24-25	0.0	1.9267142136918734E-4	0.0	3.853428427383747E-4	5.780142641075621E-4
26-27	0.0	1.9267142136918734E-4	0.0	6.743499747921557E-4	5.780142641075621E-4
28-29	0.0	1.9267142136918734E-4	0.0	0.001862490406568811	5.780142641075621E-4
30-31	0.0	1.9267142136918734E-4	0.0	0.009537235357774773	5.780142641075621E-4
32-33	0.0	1.9267142136918734E-4	0.0	0.026331760920455603	6.422380712306245E-4
34-35	0.0	1.9267142136918734E-4	0.0	0.050672583820096276	6.422380712306245E-4
36-37	0.0	1.9267142136918734E-4	0.0	0.08339461354929659	6.422380712306245E-4
38-39	6.422380712306245E-5	1.9267142136918734E-4	0.0	0.11772223845657348	7.064618783536869E-4
40-41	6.422380712306245E-5	1.9267142136918734E-4	0.0	0.1610411963610791	7.064618783536869E-4
42-43	6.422380712306245E-5	1.9267142136918734E-4	0.0	0.21794348947211242	7.706856854767494E-4
44-45	6.422380712306245E-5	1.9267142136918734E-4	0.0	0.30047108162524766	8.349094925998119E-4
46-47	6.422380712306245E-5	1.9267142136918734E-4	0.0	0.39581132329943386	8.991332997228743E-4
48-49	6.422380712306245E-5	1.9267142136918734E-4	0.0	0.47557729174627744	9.312452032844055E-4
50-51	6.422380712306245E-5	1.9267142136918734E-4	0.0	0.5501090199125914	9.633571068459367E-4
52-53	6.422380712306245E-5	1.9267142136918734E-4	0.0	0.6461878353686928	9.633571068459367E-4
54-55	6.422380712306245E-5	2.8900713205378103E-4	0.0	0.7756951424323483	9.633571068459367E-4
56-57	6.422380712306245E-5	3.2111903561531227E-4	0.0	0.9385667172964346	9.633571068459367E-4
58-59	6.422380712306245E-5	3.2111903561531227E-4	0.0	1.0897495592641235	9.633571068459367E-4
60-61	6.422380712306245E-5	3.2111903561531227E-4	0.0	1.226032477979262	9.633571068459367E-4
62-63	6.422380712306245E-5	3.2111903561531227E-4	0.0	1.380587069820912	0.0010275809139689992
64-65	6.422380712306245E-5	3.853428427383747E-4	0.0	1.5806442290092515	0.0010596928175305304
66-67	6.422380712306245E-5	3.853428427383747E-4	0.0	1.8478152666411911	0.0010918047210920615
68-69	6.422380712306245E-5	3.853428427383747E-4	0.0	2.1238170777525522	0.0010918047210920615
70-71	6.422380712306245E-5	4.174547462999059E-4	0.0	2.3650416973067747	0.0010918047210920615
72-73	6.422380712306245E-5	4.4956664986143716E-4	0.0	2.610665647648927	0.0012523642388997179
74-75	6.422380712306245E-5	4.816785534229684E-4	0.0	2.92256856694208	0.001284476142461249
76-77	6.422380712306245E-5	5.137904569844996E-4	0.0	3.318572561662883	0.0013165880460227803
78-79	6.422380712306245E-5	5.137904569844996E-4	0.0	3.746688459945217	0.0014129237567073738
80-81	1.284476142461249E-4	5.137904569844996E-4	0.0	4.132705652658384	0.001445035660268905
82-83	1.284476142461249E-4	5.137904569844996E-4	0.0	4.511658226588014	0.0016055951780765611
84-85	1.284476142461249E-4	5.137904569844996E-4	0.0	4.953678579112491	0.0016377070816380925
86-87	1.284476142461249E-4	5.137904569844996E-4	0.0	5.497750561155515	0.0017019308887611549
88-89	1.284476142461249E-4	5.137904569844996E-4	0.0	6.102610376640516	0.0017982665994457487
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2020	0.0	58.743504	1
GTATCAA	3270	0.0	36.868645	1
TCAACGC	3970	0.0	30.966665	5
CAACGCA	4090	0.0	29.817377	6
ATCAACG	4120	0.0	29.495508	4
TATCAAC	4100	0.0	29.174397	2
GTGGTAT	850	0.0	28.478853	1
AACGCAG	4305	0.0	28.219826	7
TGGTATC	900	0.0	24.260544	2
GTACATG	5260	0.0	23.732376	1
TACATGG	5310	0.0	23.062714	2
ACGCAGA	5240	0.0	22.278776	8
CGCAGAG	5380	0.0	21.346203	9
TACTACG	135	3.815029E-4	21.096123	2
ACATGGG	5500	0.0	20.883821	3
GAGTACT	2350	0.0	18.88188	12-13
CAGAGTA	5325	0.0	18.581507	10-11
CATGGGG	4705	0.0	18.160423	4
GCAGAGT	5815	0.0	17.87236	9
TTGTACG	160	0.0011964435	17.800999	4
>>END_MODULE