##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921394_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1557055
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.540655917742146	31.0	31.0	34.0	27.0	34.0
2	30.70610864741451	33.0	31.0	34.0	27.0	34.0
3	30.752207853929374	33.0	31.0	34.0	27.0	34.0
4	34.244505171622066	37.0	35.0	37.0	31.0	37.0
5	33.91689503582083	37.0	35.0	37.0	30.0	37.0
6	33.85581177286608	37.0	35.0	37.0	30.0	37.0
7	33.69720594327111	37.0	35.0	37.0	29.0	37.0
8	33.644322133771766	37.0	35.0	37.0	28.0	37.0
9	35.16425367119337	39.0	35.0	39.0	28.0	39.0
10-11	34.98991300885325	39.0	35.0	39.0	27.5	39.0
12-13	34.900713526497135	39.0	35.0	39.0	27.0	39.0
14-15	36.0204841832819	40.0	36.0	41.0	27.0	41.0
16-17	35.96176050300086	40.0	36.0	41.0	27.0	41.0
18-19	35.91516709429018	40.0	36.0	41.0	27.0	41.0
20-21	35.86564347437952	40.0	36.0	41.0	27.0	41.0
22-23	35.74255212564746	40.0	36.0	41.0	27.0	41.0
24-25	35.65650410550687	40.0	36.0	41.0	26.0	41.0
26-27	35.491936379896664	40.0	36.0	41.0	25.0	41.0
28-29	35.32135313139227	40.0	36.0	41.0	25.0	41.0
30-31	35.1508097016483	39.0	35.0	41.0	24.0	41.0
32-33	35.089251503639886	39.0	35.0	41.0	24.0	41.0
34-35	35.22027417143261	39.5	35.0	41.0	24.0	41.0
36-37	35.17859870075239	40.0	35.0	41.0	24.0	41.0
38-39	35.07626255976828	40.0	35.0	41.0	23.5	41.0
40-41	34.9297574587924	40.0	35.0	41.0	22.5	41.0
42-43	34.79475869510068	39.5	35.0	41.0	21.5	41.0
44-45	34.62590981050766	39.0	35.0	41.0	19.5	41.0
46-47	34.43758730423781	39.0	35.0	41.0	17.5	41.0
48-49	34.25481116595111	39.0	34.5	41.0	15.0	41.0
50-51	34.085151776912184	39.0	34.0	41.0	14.0	41.0
52-53	33.897593855066134	39.0	34.0	41.0	12.5	41.0
54-55	33.68040371085158	38.0	34.0	40.0	9.0	41.0
56-57	33.41106287189598	38.0	33.5	40.0	8.0	41.0
58-59	33.17040470632058	38.0	33.0	40.0	6.5	41.0
60-61	32.889427476871404	37.0	33.0	40.0	2.0	41.0
62-63	32.59508238308859	37.0	33.0	40.0	2.0	41.0
64-65	32.28649020105263	36.0	32.5	39.0	2.0	41.0
66-67	31.965460757648252	36.0	32.0	39.0	2.0	41.0
68-69	31.612061552096748	35.0	32.0	39.0	2.0	40.0
70-71	31.239043257945287	35.0	31.5	38.0	2.0	40.0
72-73	30.86217057200934	35.0	31.0	37.0	2.0	39.5
74-75	30.466809778716872	35.0	31.0	36.5	2.0	39.0
76-77	27.83841258015934	32.0	27.0	34.5	2.0	36.0
78-79	29.43847905179971	34.0	29.5	35.0	2.0	37.0
80-81	29.651919810154425	35.0	30.5	35.0	2.0	37.0
82-83	29.505578158767676	35.0	31.0	35.0	2.0	37.0
84-85	29.285718230891007	34.0	30.0	35.0	2.0	36.0
86-87	29.04374476174573	34.0	30.0	35.0	2.0	36.0
88-89	28.768054757217953	34.0	30.0	35.0	2.0	35.5
90-91	28.57517203952333	34.0	30.0	35.0	2.0	35.0
92-93	28.422375574401677	34.0	29.0	35.0	2.0	35.0
94-95	28.315096126983313	34.0	29.0	35.0	2.0	35.0
96-97	28.110471049513343	34.0	29.0	35.0	2.0	35.0
98-99	27.811815896034503	34.0	29.0	35.0	2.0	35.0
100-101	26.725597682805038	33.0	26.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	57393.0
3	15891.0
4	8177.0
5	5455.0
6	4793.0
7	4703.0
8	4621.0
9	4989.0
10	5567.0
11	5632.0
12	5944.0
13	6420.0
14	6946.0
15	7861.0
16	8516.0
17	9111.0
18	8259.0
19	7031.0
20	6613.0
21	6483.0
22	6828.0
23	7811.0
24	8947.0
25	10945.0
26	12913.0
27	15346.0
28	18493.0
29	22214.0
30	26775.0
31	32942.0
32	42278.0
33	55507.0
34	80603.0
35	120273.0
36	206838.0
37	360488.0
38	309082.0
39	28367.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.448142385000196	21.248505034657477	14.741322771054898	26.56202980928743
2	15.41701882788757	22.343890891248332	34.49839825516425	27.740692025699847
3	17.7964806272648	25.031952842100935	28.239119013578833	28.932447517055433
4	14.228323699421965	18.478998073217724	32.176043673731535	35.11663455362877
5	15.520751228718643	36.31572624964428	32.72448244784008	15.439040073796992
6	34.41354466581162	33.97649772870464	16.360134383102306	15.249823222381428
7	29.16263662018145	31.353633207326144	20.11002829702759	19.373701875464818
8	27.254335649918787	33.911612663315424	19.07133631721357	19.762715369552218
9	26.66951392211579	15.902007315091632	19.087058581745666	38.341420181046914
10-11	26.173310317764614	25.39931486795669	26.538566236340667	21.88880857793803
12-13	26.707639327974714	23.000543654701875	26.405947897135263	23.88586912018815
14-15	24.28472519715939	24.717644119515295	23.856623264518888	27.141007418806428
16-17	24.278268911502803	27.35882804396762	24.915818644813445	23.447084399716132
18-19	24.076372456052408	26.444145946173332	26.54863818102589	22.930843416748363
20-21	24.67781982315253	25.312899794996685	26.63023233708916	23.379048044761625
22-23	24.551299507017106	25.17642313808402	26.272789861867167	23.999487493031705
24-25	24.24911433834202	25.711997472107118	26.19288156667703	23.846006622873833
26-27	23.894989213376057	26.058608072723487	26.342547724147586	23.703854989752866
28-29	23.962629884018018	26.17057790600437	26.255936224683136	23.610855985294478
30-31	24.477614049421685	25.569500406912177	26.24018288245409	23.712702661212052
32-33	24.20995092974626	25.62184077523229	26.044360742640826	24.123847552380624
34-35	24.44566481042578	25.96046014220475	26.023758598762935	23.570116448606534
36-37	24.268130555412835	26.00556183527719	26.1894684786518	23.536839130658173
38-39	24.20546141216267	25.973482572670203	26.27803255062533	23.543023464541797
40-41	24.35444475344462	25.698959468668132	26.229790371568782	23.71680540631846
42-43	24.25128104996641	25.733625104854053	25.952293172572105	24.06280067260743
44-45	24.292678540461036	25.773926894593846	25.90154984488705	24.031844720058064
46-47	24.270209304401835	25.91840037921048	25.69973132834785	24.111658988039842
48-49	23.942432540843537	26.207241182963564	25.89864450619875	23.951681769994153
50-51	24.178814975858597	25.96503791813921	26.017607889670497	23.838539216331693
52-53	24.43919015255929	25.780747241229157	25.83543455056216	23.944628055649396
54-55	23.92526215565728	25.859987765266524	25.98895020527699	24.225799873799208
56-57	24.04405575788794	25.981843714906056	25.718738469275326	24.255362057930682
58-59	23.985594654067448	26.02612719198521	25.972046126909376	24.01623202703796
60-61	24.06249036574212	25.991140336453977	26.169213536260777	23.777155761543124
62-63	24.045598155415675	25.78647439720679	26.15830112729951	24.009626320078034
64-65	23.878847061922396	25.698967906485198	26.095422875663733	24.326762155928673
66-67	23.889831161822528	26.077499807262356	25.75071312928841	24.281955901626706
68-69	23.862488789986664	25.980506782539877	25.87193989120184	24.28506453627162
70-71	23.757107520318673	25.790356259436535	26.06511612965402	24.387420090590766
72-73	23.783939522029772	25.928105322529042	25.93411174908426	24.35384340635692
74-75	23.812076561829	25.96416146835903	25.785993428949432	24.437768540862535
76-77	23.695389901916712	25.737346341595273	25.903205221240107	24.664058535247907
78-79	23.6271696498197	25.84815592015065	26.017322818959933	24.507351611069716
80-81	23.72120683273875	25.91873400022618	25.868357202339954	24.491701964695118
82-83	23.657984770601892	25.849250285535152	26.055644988263833	24.437119955599123
84-85	23.604131680284322	25.933148652347036	25.95107281578971	24.511646851578934
86-87	23.508821994441746	26.13379275061637	25.73861547518967	24.618769779752217
88-89	23.428344254036315	26.01593118910924	25.78283815952757	24.772886397326882
90-91	23.39372941024534	25.805063634399065	25.974274420990895	24.8269325343647
92-93	23.364903315924384	25.6997070452109	26.102564053139858	24.832825585724855
94-95	23.282742063522647	25.672002610167837	26.133823767427877	24.911431558881635
96-97	23.216700033591724	25.621109734471215	26.08092458818021	25.08126564375685
98-99	23.0146303242049	25.668062477521453	25.918634074911374	25.398673123362276
100-101	22.928581658624683	25.70048110610088	25.979183538991045	25.39175369628339
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	36.0
1	28.0
2	32.5
3	39.0
4	32.5
5	35.0
6	63.5
7	185.5
8	312.0
9	348.0
10	314.5
11	260.0
12	245.0
13	261.0
14	301.0
15	354.0
16	403.5
17	439.5
18	492.5
19	560.5
20	630.0
21	700.0
22	811.0
23	986.0
24	1245.0
25	1557.5
26	1957.5
27	2671.5
28	3743.5
29	5157.5
30	7064.5
31	9698.0
32	13267.0
33	16110.5
34	18472.5
35	21526.5
36	25403.5
37	29839.0
38	33595.0
39	37063.0
40	40395.5
41	43910.0
42	47661.5
43	51042.0
44	54630.0
45	58167.5
46	61429.5
47	64777.0
48	67312.0
49	68680.5
50	69244.5
51	69073.0
52	68227.0
53	67406.0
54	65955.5
55	62512.5
56	58142.5
57	52866.0
58	46916.0
59	40689.5
60	34006.5
61	28421.0
62	23467.5
63	18131.5
64	13620.0
65	10348.5
66	7992.5
67	6423.5
68	5060.5
69	3841.0
70	2880.0
71	2139.5
72	1542.5
73	1130.0
74	858.0
75	667.5
76	485.5
77	306.5
78	193.0
79	109.0
80	59.5
81	37.0
82	23.5
83	13.0
84	8.0
85	7.0
86	6.5
87	4.0
88	2.0
89	2.5
90	1.5
91	1.0
92	1.5
93	0.5
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11785068607081958
2	1.9267142136918734E-4
3	0.004688337919983559
4	0.0035323093917684345
5	0.02260678010731798
6	8.991332997228742E-4
7	6.422380712306245E-5
8	0.0014129237567073738
9	0.0
10-11	0.08326616593505046
12-13	3.2111903561531224E-5
14-15	0.004238771270122121
16-17	0.0
18-19	9.633571068459367E-5
20-21	4.495666498614371E-4
22-23	1.9267142136918734E-4
24-25	0.0017340427923226863
26-27	0.002183609442184123
28-29	0.0057159188339525584
30-31	0.01335855188159699
32-33	0.013197992363789334
34-35	0.009312452032844056
36-37	9.633571068459367E-4
38-39	2.2478332493071855E-4
40-41	0.003981876041629872
42-43	0.008284871118875055
44-45	0.008027975890382807
46-47	0.008734437768736492
48-49	0.010821711500236022
50-51	0.005298464087652653
52-53	0.0013486999495843114
54-55	8.027975890382806E-4
56-57	0.00472044982354509
58-59	0.008541766347367306
60-61	0.007128842590659932
62-63	0.01807900170514208
64-65	0.03275414163276185
66-67	0.03435973681083841
68-69	0.026395984727578668
70-71	0.03885540330945278
72-73	0.02498306097087129
74-75	0.024308710996079137
76-77	0.21351204678062113
78-79	0.05776931450719468
80-81	0.0502872409773579
82-83	0.021129632543487546
84-85	0.031919232140162034
86-87	0.07520607814110612
88-89	0.24485326465667556
90-91	0.29244310573486487
92-93	0.04312628648313643
94-95	0.004302995077245184
96-97	0.007963752083259744
98-99	9.633571068459367E-4
100-101	0.0011560285282151241
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1557055.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.58955597799903
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.87161242401449	62.45664273420051
2	9.383689213359508	13.8108304529333
3	2.5723587297472656	5.678962102146926
4	1.1058019398078787	3.255018950002872
5	0.6205440855760499	2.283278186115747
6	0.361645600352663	1.5968003490809612
7	0.253309353144416	1.3048645976080206
8	0.17540739819111217	1.0326522038512012
9	0.1306910830732511	0.8655748896279899
>10	0.505513451571339	5.894207969689156
>50	0.012676901235998477	0.6250299300436423
>100	0.00631155182379424	0.9365093965365701
>500	2.6296086141047476E-4	0.11223680357857603
>1k	1.7530724094031648E-4	0.14739143458450932
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.422380712306245E-5	0.0
5	0.0	0.0	0.0	6.422380712306245E-5	0.0
6	0.0	0.0	0.0	6.422380712306245E-5	0.0
7	0.0	0.0	0.0	6.422380712306245E-5	0.0
8	0.0	0.0	0.0	1.284476142461249E-4	0.0
9	0.0	0.0	0.0	1.284476142461249E-4	0.0
10-11	0.0	0.0	0.0	1.284476142461249E-4	0.0
12-13	0.0	0.0	0.0	1.284476142461249E-4	0.0
14-15	0.0	0.0	0.0	1.284476142461249E-4	9.633571068459367E-5
16-17	0.0	0.0	0.0	1.284476142461249E-4	1.9267142136918734E-4
18-19	0.0	0.0	0.0	1.284476142461249E-4	2.568952284922498E-4
20-21	0.0	0.0	0.0	1.6055951780765613E-4	2.568952284922498E-4
22-23	0.0	0.0	0.0	2.568952284922498E-4	3.2111903561531227E-4
24-25	0.0	0.0	0.0	5.137904569844996E-4	4.174547462999059E-4
26-27	0.0	0.0	0.0	8.027975890382807E-4	4.4956664986143716E-4
28-29	0.0	6.422380712306245E-5	0.0	0.001862490406568811	4.4956664986143716E-4
30-31	0.0	6.422380712306245E-5	0.0	0.008766549672298025	4.4956664986143716E-4
32-33	0.0	6.422380712306245E-5	0.0	0.024308710996079137	5.780142641075621E-4
34-35	0.0	6.422380712306245E-5	0.0	0.046947603006958646	7.064618783536869E-4
36-37	0.0	6.422380712306245E-5	0.0	0.07658688999425198	7.064618783536869E-4
38-39	0.0	6.422380712306245E-5	0.0	0.10898780068783698	7.385737819152181E-4
40-41	0.0	6.422380712306245E-5	0.0	0.1500589253430354	8.349094925998119E-4
42-43	0.0	6.422380712306245E-5	0.0	0.20419959474777705	8.349094925998119E-4
44-45	0.0	6.422380712306245E-5	0.0	0.2813644990061366	8.349094925998119E-4
46-47	0.0	6.422380712306245E-5	0.0	0.3700575766430858	8.349094925998119E-4
48-49	0.0	6.422380712306245E-5	0.0	0.44519943097706893	8.67021396161343E-4
50-51	0.0	6.422380712306245E-5	0.0	0.5161346259444913	9.95469010407468E-4
52-53	3.2111903561531224E-5	6.422380712306245E-5	0.0	0.6069149773129401	0.0010596928175305304
54-55	6.422380712306245E-5	1.284476142461249E-4	0.0	0.7305458060248353	0.0010918047210920615
56-57	6.422380712306245E-5	1.9267142136918734E-4	0.0	0.8865133216231925	0.0010918047210920615
58-59	6.422380712306245E-5	1.9267142136918734E-4	0.0	1.0312416709750138	0.0010918047210920615
60-61	6.422380712306245E-5	1.9267142136918734E-4	0.0	1.1606847542315462	0.0010918047210920615
62-63	6.422380712306245E-5	1.9267142136918734E-4	0.0	1.3075324892184286	0.0011560285282151241
64-65	6.422380712306245E-5	2.568952284922498E-4	0.0	1.497923965434747	0.0011560285282151241
66-67	6.422380712306245E-5	2.568952284922498E-4	0.0	1.7526034725812512	0.0011560285282151241
68-69	6.422380712306245E-5	2.568952284922498E-4	0.0	2.016755991278407	0.0011560285282151241
70-71	6.422380712306245E-5	2.8900713205378103E-4	0.0	2.247865361210747	0.0011560285282151241
72-73	6.422380712306245E-5	3.2111903561531227E-4	0.0	2.4839199642915633	0.0013165880460227803
74-75	6.422380712306245E-5	3.2111903561531227E-4	0.0	2.7862214244198182	0.0013486999495843114
76-77	6.422380712306245E-5	3.2111903561531227E-4	0.0	3.1685136363198474	0.0013486999495843114
78-79	6.422380712306245E-5	3.2111903561531227E-4	0.0	3.583527877949077	0.0013486999495843114
80-81	6.422380712306245E-5	3.2111903561531227E-4	0.0	3.958691247258446	0.0014129237567073738
82-83	6.422380712306245E-5	3.2111903561531227E-4	0.0	4.328556152480163	0.0015413713709534988
84-85	6.422380712306245E-5	3.2111903561531227E-4	0.0	4.758309757844135	0.0015413713709534988
86-87	6.422380712306245E-5	3.2111903561531227E-4	0.0	5.284495409603386	0.00157348327451503
88-89	6.422380712306245E-5	3.2111903561531227E-4	0.0	5.873106601886253	0.0016698189851996237
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1830	0.0	52.97339	1
GTATCAA	3170	0.0	39.875034	1
TATCAAC	4050	0.0	31.055536	2
ATCAACG	4095	0.0	30.598366	3
TCAACGC	4155	0.0	30.042284	4
CAACGCA	4240	0.0	29.259378	5
AACGCAG	4445	0.0	27.907265	6
GTGGTAT	855	0.0	26.12224	1
TGGTATC	960	0.0	22.742321	2
ACGCAGA	5380	0.0	22.349756	7
CGCAGAG	5675	0.0	21.02047	8
GTACATG	4935	0.0	19.836212	1
GTAAGTA	1025	0.0	18.984896	2
TACATGG	5155	0.0	18.96648	2
TAAGTAC	1255	0.0	18.909258	3
GCAGAGT	6160	0.0	17.822386	9
GTACACT	1510	0.0	17.626238	6
ACATGGG	5415	0.0	17.266964	3
CAGAGTA	5730	0.0	17.086266	10-11
AGTACAC	1515	0.0	16.942268	5
>>END_MODULE