FastQCFastQC Report
Mon 15 Aug 2016
SRR2921393_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921393_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2155713
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18050.048.8981
GTATCAA32150.042.2124371
TCAACGC39500.033.279624
CAACGCA40900.032.2534985
ATCAACG41150.031.943723
TATCAAC42750.031.5266552
AACGCAG43250.030.3821136
GTACATG55000.027.8673171
TACATGG57550.025.8103982
ACGCAGA51700.024.6857627
ACATGGG58700.024.3334643
CGCAGAG52550.023.9292188
CATGGGG48200.022.1529314
GTATACG1100.002895075421.5691341
TATACCG2005.44318E-721.3534415
GCAGAGT59750.019.5372149
AGAGTAC51350.018.80613110-11
ACTGCGT3951.8189894E-1118.0197838
ATGGGGG30450.017.609485
TAAGGTG10850.017.4938585
Produced by FastQC (version 0.11.5)